Lipossarcoma pleomórfico esplênico primário em uma cadela / Primary splenic pleomorphic liposarcoma in a bitch

Background: Liposarcoma is a malignant neoplasm of lipoblasts with low incidence in dogs, representing 1.7% of tumorsdiagnosed in the spleen. In veterinary medicine, this neoplasm is classified morphologically into the myxoid, well-differentiated, undifferentiated and pleomorphic subtypes, the latter being one of the most aggressive forms, mainly in cavityorgans. This report refers to a primary splenic pleomorphic liposarcoma in a female dog, addressing anatomopathologicaland immunohistochemical aspects.Case: A 14-year-old, 35 kg bitch mongrel with history of absence of defecation, progressive weight loss, difficulty walking, sensitivity to abdominal palpation, prostration, pale mucous membranes, tachypnea and abdominal distention waspresented to diagnosis. The condition evolved to death and, on necroscopy, there was an increase in splenic volume withneoformation of whitish and reddish color, measuring 32 × 27 cm in its largest axes and weighing 8.9 kg. The neoformationexhibited areas of firm and soft consistency, and sectioning revealed focal areas of extensive necrosis and cavity collectionsof different diameters that allowed the flow of liquid serous contents with a brownish red color. Microscopy showed cellsof neoplastic morphology infiltrating the splenic parenchyma, mostly with slightly acidophilic cytoplasm and few intracytoplasmic lipid vacuoles, which varied in size and distribution. The nucleus of the cells was large, eccentric and irregular,with round to oval morphology, grossly lacy chromatin and single or multiple evident nucleoli. These cells exhibited markedanisocytosis, anisokaryosis and pleomorphism, with more than one mitotic figure per high magnification field visible. Mildinflammatory infiltrate, predominantly lymphocytic, permeated the neoplastic cells, and marked depletion of lymphoidfollicles and atrophy of the red pulp were found in the remaining splenic parenchyma. Immunohistochemical tests...

Lipossarcoma pleomórfico esplênico primário em uma cadela / Primary splenic pleomorphic liposarcoma in a bitch

Background: Liposarcoma is a malignant neoplasm of lipoblasts with low incidence in dogs, representing 1.7% of tumorsdiagnosed in the spleen. In veterinary medicine, this neoplasm is classified morphologically into the myxoid, well-differentiated, undifferentiated and pleomorphic subtypes, the latter being one of the most aggressive forms, mainly in cavityorgans. This report refers to a primary splenic pleomorphic liposarcoma in a female dog, addressing anatomopathologicaland immunohistochemical aspects.Case: A 14-year-old, 35 kg bitch mongrel with history of absence of defecation, progressive weight loss, difficulty walking, sensitivity to abdominal palpation, prostration, pale mucous membranes, tachypnea and abdominal distention waspresented to diagnosis. The condition evolved to death and, on necroscopy, there was an increase in splenic volume withneoformation of whitish and reddish color, measuring 32 × 27 cm in its largest axes and weighing 8.9 kg. The neoformationexhibited areas of firm and soft consistency, and sectioning revealed focal areas of extensive necrosis and cavity collectionsof different diameters that allowed the flow of liquid serous contents with a brownish red color. Microscopy showed cellsof neoplastic morphology infiltrating the splenic parenchyma, mostly with slightly acidophilic cytoplasm and few intracytoplasmic lipid vacuoles, which varied in size and distribution. The nucleus of the cells was large, eccentric and irregular,with round to oval morphology, grossly lacy chromatin and single or multiple evident nucleoli. These cells exhibited markedanisocytosis, anisokaryosis and pleomorphism, with more than one mitotic figure per high magnification field visible. Mildinflammatory infiltrate, predominantly lymphocytic, permeated the neoplastic cells, and marked depletion of lymphoidfollicles and atrophy of the red pulp were found in the remaining splenic parenchyma. Immunohistochemical tests...(AU)

Imunomarcação de TGF-β em próstatas caninas normais e com lesões proliferativas / Immunoexpression of TGF-β in normal canine prostates and with proliferative lesions

O fator de crescimento transformador-β (TGF-β), um mediador do crescimento prostático, induz a angiogênese e inibe a proliferação celular. Neste estudo, esse marcador foi utilizado com o objetivo de avaliar sua imunomarcação no tecido normal e com lesões proliferativas benignas, pré-neoplásicas e neoplásicas da próstata canina. Para isso, foram selecionadas 54 glândulas com histomorfologia normal, hiperplasia prostática benigna (HPB) epitelial, HPB estromal, atrofia inflamatória proliferativa (PIA), neoplasia intraepitelial prostática (PIN) e carcinoma, utilizadas para a confecção de um bloco de microarranjo tecidual (Tissue Microarray - TMA). As lâminas de TMA foram submetidas à técnica de imunoistoquímica com o anticorpo anti-TGF-β, sendo avaliada a intensidade de imunomarcação nas células epiteliais e estromais. Houve imunomarcação de TGF-β no tecido normal e naqueles com lesões proliferativas. Maior imunomarcação de TGF-β foi constatada nas células do tecido prostático normal e com HPB, enquanto as células prostáticas com PIA, PIN e carcinoma exibiram menor imunomarcação dessa citocina, o que sugere a ação do TGF-β na manutenção da homeostase do tecido normal e com lesão proliferativa benigna e na progressão das lesões proliferativas pré-malignas e malignas da próstata canina.(AU)

The transforming growth factor-β (TGF-β), a mediator of prostatic growth induces angiogenesis and inhibits cell proliferation. It was used in this study in order to evaluate its expression in normal prostatic tissue and those with benign proliferative lesions, pre-malignant and malignant prostatic diseases. A total of 54 glands with normal histomorphology, epithelial benign prostatic hyperplasia (BPH), stromal BPH, prostatic inflammatory atrophy (PIA), prostatic intraepithelial neoplasia (PIN), and adenocarcinoma were selected and used in the tissue microarray block (TMA). The TMA slides were subjected to the immunohistochemistry with anti-TGF-β and its staining intensity in epithelial and stromal cells was evaluated. There was TGF-β immunostaining by both normal tissues and those with proliferative lesions. Higher TGF-β immunostaining was observed in cells from normal prostatic tissues and with HPB, whereas prostatic cells with PIA, PIN and carcinoma exhibited lower immunostaining of this cytokine, suggesting the action of TGF-β in the maintenance of normal tissue homeostasis and with benign proliferative lesion and in the progression of pre-malignant and malignant proliferative lesions of the canine prostate.(AU)

Imunomarcação de TGF-β em próstatas caninas normais e com lesões proliferativas / Immunoexpression of TGF-β in normal canine prostates and with proliferative lesions

O fator de crescimento transformador-β (TGF-β), um mediador do crescimento prostático, induz a angiogênese e inibe a proliferação celular. Neste estudo, esse marcador foi utilizado com o objetivo de avaliar sua imunomarcação no tecido normal e com lesões proliferativas benignas, pré-neoplásicas e neoplásicas da próstata canina. Para isso, foram selecionadas 54 glândulas com histomorfologia normal, hiperplasia prostática benigna (HPB) epitelial, HPB estromal, atrofia inflamatória proliferativa (PIA), neoplasia intraepitelial prostática (PIN) e carcinoma, utilizadas para a confecção de um bloco de microarranjo tecidual (Tissue Microarray - TMA). As lâminas de TMA foram submetidas à técnica de imunoistoquímica com o anticorpo anti-TGF-β, sendo avaliada a intensidade de imunomarcação nas células epiteliais e estromais. Houve imunomarcação de TGF-β no tecido normal e naqueles com lesões proliferativas. Maior imunomarcação de TGF-β foi constatada nas células do tecido prostático normal e com HPB, enquanto as células prostáticas com PIA, PIN e carcinoma exibiram menor imunomarcação dessa citocina, o que sugere a ação do TGF-β na manutenção da homeostase do tecido normal e com lesão proliferativa benigna e na progressão das lesões proliferativas pré-malignas e malignas da próstata canina.

The transforming growth factor-β (TGF-β), a mediator of prostatic growth induces angiogenesis and inhibits cell proliferation. It was used in this study in order to evaluate its expression in normal prostatic tissue and those with benign proliferative lesions, pre-malignant and malignant prostatic diseases. A total of 54 glands with normal histomorphology, epithelial benign prostatic hyperplasia (BPH), stromal BPH, prostatic inflammatory atrophy (PIA), prostatic intraepithelial neoplasia (PIN), and adenocarcinoma were selected and used in the tissue microarray block (TMA). The TMA slides were subjected to the immunohistochemistry with anti-TGF-β and its staining intensity in epithelial and stromal cells was evaluated. There was TGF-β immunostaining by both normal tissues and those with proliferative lesions. Higher TGF-β immunostaining was observed in cells from normal prostatic tissues and with HPB, whereas prostatic cells with PIA, PIN and carcinoma exhibited lower immunostaining of this cytokine, suggesting the action of TGF-β in the maintenance of normal tissue homeostasis and with benign proliferative lesion and in the progression of pre-malignant and malignant proliferative lesions of the canine prostate.

Correlation between clinical findings, mast cell count and interleukin 31 immunostaining in the skin of dogs with atopic dermatitis / Correlação entre achados clínicos, contagem de mastócitos e imunomarcação de interleucina 31 na pele de cães com dermatite atópica

In this study the correlation between the clinical score, mast cell count and interleukin 31 (IL-31) immunostaining in the skin of dogs with atopic dermatitis was determined. A total of 31 dogs of different breeds, from one to eight years of age, were chosen for the study. The 20 females and 11 males were categorized based on the CADESI-4 system, as having discrete, moderate or marked atopic dermatitis. Skin samples were collected from the axillary and interdigital regions and stained with hematoxylin and eosin for cytohistomorphological analyses and toluidine blue to evaluate the mast cell counts, and immunohistochemistry for the IL-31 immunostaining. Animals revealing higher atopic dermatitis scores had greater numbers of mast cells and IL-31 immunolabeled cells. More numbers of cells immunolabeled for IL-31 were evident in the axillary skin compared with the interdigital skin in dogs having this condition. A correlation was identified between the clinical scores and mast cell numbers in the interdigital region, as well as between the clinical scores and number of cells immunolabeled for IL-31 in the axillary area. A correlation was also reported between the mast cell numbers and IL-31 immunolabeled cells only in the axillary skin, and none in the interdigital regions. It was thus concluded that the mast cells and IL-31 are involved in the pathogenesis of the canine atopic dermatitis (CAD), as well as lymphocytes and plasma cells. It was also observed that the higher the degree of clinical severity of the disease, the more the numbers of mast cells and IL-31 in the skin of those animals suffering from CAD, which implies the influence of these immunological constituents on the genesis of pruritus and disease progression.(AU)

Este estudo avaliou a correlação entre o escore clínico, a contagem de mastócitos e a imunomarcação de interleucina 31 (IL-31) na pele de cães com dermatite atópica. Foram selecionados 31 cães de diferentes raças, com idade entre um e oito anos, sendo 20 fêmeas e 11 machos, divididos em discretamente, moderadamente e acentuadamente acometidos por dermatite atópica segundo o sistema CADESI-4. Amostras da pele das regiões axilar e interdigital foram colhidas e submetidas às colorações de hematoxilina e eosina para a avaliação cito-histomorfológica e azul de toluidina para a contagem de mastócitos, bem como a técnica de imunoistoquímica para a imunomarcação de IL-31. Os animais com maior escore de dermatite atópica apresentaram maior número de mastócitos e de células imunomarcadas para IL-31. Houve maior número de células imunomarcadas para IL-31 na pele da axila em relação à interdigital nos cães com a doença. Foi constatada correlação entre o escore clínico e a quantidade de mastócitos no interdígito, bem como entre o escore clínico e a quantidade de células imunomarcadas para IL-31 na axila. Também foi verificada correlação entre a quantidade de mastócitos e células imunomarcadas para IL-31 na pele da região axilar, mas não da interdigital. Conclui-se que mastócitos e a IL-31 estão envolvidos na patogenia da DAC, assim como linfócitos e plasmócitos. Também, quanto maior o grau de severidade clínica da doença, maior a quantidade de mastócitos e IL-31 na pele dos animais com DAC, o que remete à influência desses componentes imunológicos na gênese do prurido e progressão da doença.(AU)

Acaricidal activity of ethanolic extracts of Stryphnodendron adstringens and Lafoensia pacari and oil resins from Copaifera sp. and Pterodon emarginatus against Sarcoptes scabiei var. suis / Atividade acaricida dos extratos etanólicos de Stryphnodendron adstringens Mart. e Lafoensia pacari A. St Hil., bem como das óleo-resinas de Copaifera sp. e Pterodon emarginatus Vogel, 1837 contra Sarcoptes scabiei var. suis

In vitro bioassay using Sarcoptes scabiei as a test microorganism is a viable method of study for diverse drugs with acaricidal properties. A great amount of assays proves the diverse and efficient biological activity of extracts and compounds from Brazilian savanna plants. This study had as main object, test and compare the acaricidal activity of four Brazilian Cerrado bioproducts: Stryphnodendron adstringens Mart., Copaifera sp., Lafoensia pacari A. St Hil. and Pterodon emarginatus Vogel, 1837. To perform this test S. scabiei mites were collected from crusts removed on ears of highly infected sows. The mites were selected and separated in Petri dishes with three different concentrations (25, 50 and 75%) of each bioproduct, to evaluate their potential acaricidal activity. The mortality of the mites was counted in each Petri dish every hour, during five hours. The statistical analyses demonstrated differences between the bioproducts tested. The oleoresin of Copaifera sp. and P. emarginatus presented the best results with 100% of mites mortality after treatment. The ethanolic extracts of S. adstringens and L. pacari demonstrated lower acaricidal activity when compared to the oleoresins, with little or no difference among the control groups tested. This bioassay demonstrated to be efficient, reliable, low cost and easy accomplishment. Oil resins from Copaifera sp. and P. emarginatus have in vitro acaricidal activity against adult females of S. scabiei var. suis.(AU)

O uso de Sarcoptes scabiei como microrganismo teste para bioensaio in vitro é um método de estudo viável para diversas drogas acaricidas. Muitos ensaios comprovam a diversidade e eficiência de atividade biocida de extratos e componentes presentes em plantas do cerrado brasileiro. Este trabalho objetivou testar e comparar a atividade acaricida de quatro bioprodutos do cerrado brasileiro: Stryphnodendron adstringens Mart., Lafoensia pacari A. St Hil., Copaifera sp. e Pterodon emarginatus Vogel, 1837. Para este estudo, ácaros S. scabiei foram colhidos em crostas removidas de orelhas de fêmeas suínas altamente infestadas. Os ácaros foram selecionados, separados e colocados em placas de Petri com três diferentes concentrações (25, 50 e 75%) de bioprodutos, para avaliar o potencial de atividade acaricida de cada um. Foi realizada a contagem da mortalidade dos ácaros em cada placa de Petri a cada hora, durante cinco horas. As análises estatísticas demonstraram diferenças entre os bioprodutos testados. As óleo-resinas de Copaifera sp. e P. emarginatus apresentaram os melhores resultados, com 100% de mortalidade dos ácaros após tratamento. Os extratos etanólicos de S. adstringens Mart. e L. pacari demonstraram menor atividade acaricida quando comparados as óleo-resinas, com pequena ou nenhuma diferença entre os resultados dos grupos controle. Este ensaio demonstrou ser uma ferramenta eficiente, confiável, de baixo custo e de fácil realização. As óleo-resinas Copaifera sp. e P. emarginatus possuem atividade acaricida in vitro sobre fêmeas adultas de S. scabiei var. suis.(AU)

Acaricidal activity of ethanolic extracts of Stryphnodendron adstringens and Lafoensia pacari and oil resins from Copaifera sp. and Pterodon emarginatus against Sarcoptes scabiei var. suis / Atividade acaricida dos extratos etanólicos de Stryphnodendron adstringens Mart. e Lafoensia pacari A. St Hil., bem como das óleo-resinas de Copaifera sp. e Pterodon emarginatus Vogel, 1837 contra Sarcoptes scabiei var. suis

In vitro bioassay using Sarcoptes scabiei as a test microorganism is a viable method of study for diverse drugs with acaricidal properties. A great amount of assays proves the diverse and efficient biological activity of extracts and compounds from Brazilian savanna plants. This study had as main object, test and compare the acaricidal activity of four Brazilian Cerrado bioproducts: Stryphnodendron adstringens Mart., Copaifera sp., Lafoensia pacari A. St Hil. and Pterodon emarginatus Vogel, 1837. To perform this test S. scabiei mites were collected from crusts removed on ears of highly infected sows. The mites were selected and separated in Petri dishes with three different concentrations (25, 50 and 75%) of each bioproduct, to evaluate their potential acaricidal activity. The mortality of the mites was counted in each Petri dish every hour, during five hours. The statistical analyses demonstrated differences between the bioproducts tested. The oleoresin of Copaifera sp. and P. emarginatus presented the best results with 100% of mites mortality after treatment. The ethanolic extracts of S. adstringens and L. pacari demonstrated lower acaricidal activity when compared to the oleoresins, with little or no difference among the control groups tested. This bioassay demonstrated to be efficient, reliable, low cost and easy accomplishment. Oil resins from Copaifera sp. and P. emarginatus have in vitro acaricidal activity against adult females of S. scabiei var. suis.(AU)

O uso de Sarcoptes scabiei como microrganismo teste para bioensaio in vitro é um método de estudo viável para diversas drogas acaricidas. Muitos ensaios comprovam a diversidade e eficiência de atividade biocida de extratos e componentes presentes em plantas do cerrado brasileiro. Este trabalho objetivou testar e comparar a atividade acaricida de quatro bioprodutos do cerrado brasileiro: Stryphnodendron adstringens Mart., Lafoensia pacari A. St Hil., Copaifera sp. e Pterodon emarginatus Vogel, 1837. Para este estudo, ácaros S. scabiei foram colhidos em crostas removidas de orelhas de fêmeas suínas altamente infestadas. Os ácaros foram selecionados, separados e colocados em placas de Petri com três diferentes concentrações (25, 50 e 75%) de bioprodutos, para avaliar o potencial de atividade acaricida de cada um. Foi realizada a contagem da mortalidade dos ácaros em cada placa de Petri a cada hora, durante cinco horas. As análises estatísticas demonstraram diferenças entre os bioprodutos testados. As óleo-resinas de Copaifera sp. e P. emarginatus apresentaram os melhores resultados, com 100% de mortalidade dos ácaros após tratamento. Os extratos etanólicos de S. adstringens Mart. e L. pacari demonstraram menor atividade acaricida quando comparados as óleo-resinas, com pequena ou nenhuma diferença entre os resultados dos grupos controle. Este ensaio demonstrou ser uma ferramenta eficiente, confiável, de baixo custo e de fácil realização. As óleo-resinas Copaifera sp. e P. emarginatus possuem atividade acaricida in vitro sobre fêmeas adultas de S. scabiei var. suis.(AU)

Mortality of Cebus apella by Molineus torulosus parasitism in Midwestern Brazil

Background: Wild and captivity monkeys are infested by several parasites species, mainly, Strongyloides sp. and Acanthocephala sp, which has been identifi ed as the major causes of illness and death. However, Molineus torulosus is a pathogenic nematode found in Neotropical New World Primates and causes severe illness in small primates due to severe gastrointestinal injury. In South America, the parasite was described in Cebus apella and C. olivecaeus from French Guyana and were verified the occurrence in tropical forests. Thus, it is the first report about M. torulosus infestation in capitivity capuchin monkeys in Brazil. Case: Two capuchin monkeys were presented for necropsy. According to veterinarian the monkeys had about fi ve years old and weighed 0.9 kg and lived in a particular park in urban with 20 to 30 capuchin monkeys group. The veterinarian described that six capuchin monkeys were found dead in two months period. One of the monkeys were found dead and another one had respiratory disturbance, apathy, prostration, cyanotic mucosa, feces with green coloration and death after four days in observation and isolation of the group. Necropsy confirmed emaciation and moderate splenomegaly, great amount of fibrin on the intestine and presence of multifocal 1 to 2 cm nodules in small intestine with numerous parasites mainly initial portion. In the small intestine, were found several free helminthes in the feces. No parasite was attached in the gut wall. The parasites in the nodules were submitted to a standard nematode key identification, confirming the infestation by M. torulosus. The parasites were small, slender, pale red, of 4 to 6 mm length. There were no significant macroscopic findings in the other organ systems. The histological examination of nodules sections revealed an intense granulomatous inflammatory response surrounded by proliferating fibrous connective tissue and the central portion contained a mass of nematode parasites and their eggs surrounded by eosinophilic debris. The small intestine revealed the presence of eggs and larvae at the villi, epithelium and crypts necrosis areas, villi atrophy and congestion, mononuclear inflammatory reaction. Discussion: There are no reports of Molineus torulosus parasitism in captivity capuchin monkeys in Brazil. The clinical signs described in the present case were unspecific and the parasite identification was a necropsy found. However, the infestation with M. torulosus was associated with enteritis and diarrhea. The parasite causes hemorrhagic or ulcerative enteritis, sometimes associated with diverticula of the intestinal wall. Furthermore, the capuchin monkeys death in the present case was assigned with a fibrinous peritonitis and septicemia because a gut wall necrosis caused by M. torulosus infestation. Thus, the high density of M. torulosus in the small intestine is believed to be responsible for the death of these capuchin monkeys. The present report identifies the nematode M. torulosus and associated with capuchin monkey mortality. Thus, the present case also highlights the importance of performing a complete necropsy on monkeys, including thorough examination of the gastrointestinal system, and stresses the value of saving fresh tissues for diagnostic procedures including parasite recovery and identification.

Avaliações histológica e histomorfométrica de testículos de bovinos com dermatite digital / Testis histologic and histomorphometric evaluations of cattle with digital dermatitis

A infecção da região digital é a causa mais comum de claudicação em bovinos, podendo levar à inutilização de animais de alta produção e destinados à reprodução. Este estudo teve como objetivo avaliar aspectos histológicos e histomorfométricos dos testículos de bovinos com dermatite digital. Foram analisadas diferentes porções dos testículos de 20 bovinos da raça Nelore, entre 25 e 30 meses, sendo 10 com de dermatite digital e 10 saudáveis. Todas as amostras testiculares avaliadas apresentaram algum grau de degeneração do epitélio tubular seminífero. Infiltrado inflamatório intersticial mononuclear foi observado em testículos de bovinos saudáveis e com dermatite digital. A altura do epitélio tubular seminífero de todas as regiões testiculares foi maior nos animais com dermatite digital, assim como foram observados maior área da luz tubular e maior diâmetro tubular nos testículos desses bovinos. Concluiu-se que bovinos jovens da raça Nelore apresentam diferentes graus de degeneração testicular, bem como orquite intersticial crônica inespecífica, sem relação com a dermatite digital. Ainda, as alterações histomorfométricas dos testículos desses bovinos não possuem relação com a enfermidade podal.

The infection of the digital area is the most common cause of lameness in cattle, leading to the rejection of high-production animals destined to reproduction. This study aimed at histological and histomorphometric evaluations of the testis of bovines with digital dermatitis. Different portions of testis from 20 Nellore animals, ten healthy and ten with digital dermatitis, between 25 and 30 months of age, were analyzed. All analyzed testis showed some degree of degeneration of the seminiferous epithelium. Mononuclear interstitial infiltrate was observed in testis of both healthy bovines and the ones with digital dermatitis. Bovines with digital dermatitis presented higher height of seminiferous tubular epithelium in all regions of testis, larger area of the tubular lumen, and larger tubular diameter. We concluded that young Nellore bovines show different degrees of testicular degeneration, as well as chronic nonspecific interstitial orchitis, unrelated to digital dermatitis. The testis histomorphometric changes of these animals are not related with foot disease.

Avaliações histológica e histomorfométrica de testículos de bovinos com dermatite digital / Testis histologic and histomorphometric evaluations of cattle with digital dermatitis

A infecção da região digital é a causa mais comum de claudicação em bovinos, podendo levar à inutilização de animais de alta produção e destinados à reprodução. Este estudo teve como objetivo avaliar aspectos histológicos e histomorfométricos dos testículos de bovinos com dermatite digital. Foram analisadas diferentes porções dos testículos de 20 bovinos da raça Nelore, entre 25 e 30 meses, sendo 10 com de dermatite digital e 10 saudáveis. Todas as amostras testiculares avaliadas apresentaram algum grau de degeneração do epitélio tubular seminífero. Infiltrado inflamatório intersticial mononuclear foi observado em testículos de bovinos saudáveis e com dermatite digital. A altura do epitélio tubular seminífero de todas as regiões testiculares foi maior nos animais com dermatite digital, assim como foram observados maior área da luz tubular e maior diâmetro tubular nos testículos desses bovinos. Concluiu-se que bovinos jovens da raça Nelore apresentam diferentes graus de degeneração testicular, bem como orquite intersticial crônica inespecífica, sem relação com a dermatite digital. Ainda, as alterações histomorfométricas dos testículos desses bovinos não possuem relação com a enfermidade podal.(AU)

The infection of the digital area is the most common cause of lameness in cattle, leading to the rejection of high-production animals destined to reproduction. This study aimed at histological and histomorphometric evaluations of the testis of bovines with digital dermatitis. Different portions of testis from 20 Nellore animals, ten healthy and ten with digital dermatitis, between 25 and 30 months of age, were analyzed. All analyzed testis showed some degree of degeneration of the seminiferous epithelium. Mononuclear interstitial infiltrate was observed in testis of both healthy bovines and the ones with digital dermatitis. Bovines with digital dermatitis presented higher height of seminiferous tubular epithelium in all regions of testis, larger area of the tubular lumen, and larger tubular diameter. We concluded that young Nellore bovines show different degrees of testicular degeneration, as well as chronic nonspecific interstitial orchitis, unrelated to digital dermatitis. The testis histomorphometric changes of these animals are not related with foot disease.(AU)

Dois casos de leucemia linfone canina diagnosticados ao exame post mortem / Two canine lymphoid leukemias diagnosed in post mortem exam

Leucemias são neoplasias mesenquimais malignas com origem nas células da medula óssea e/ou sangue e classificadas em mieloide ou linfoide a depender do tipo celular envolvido. Dois casos de leucemia linfoide canina foram diagnosticados ao exame post mortem. O primeiro, um cão, Bull Terrier, de dois anos e histórico de aumento de volume abdominal, diarréia escura e icterícia. O segundo, uma cadela, Dálmata, de nove anos, que apresentava emagrecimento progressivo e lesões cutâneas havia seis meses. Ao exame macroscópico, os dois animais apresentaram linfadenomegalia generalizada e hepatoesplenomegalia. A partir dos exames histopatológico e imunoistoquímico, utilizando os anticorpos anti-CD3 e anti-CD79a, foram confirmados os diagnósticos de leucemia linfóide aguda e crônica, respectivamente, ambas de imunofenótipo T.(AU)

Leukemias are malignant mesenchymal neoplasias from the bone marrow cells and/or blood cells and it may be myeloid or limphoyd depending cell type involved. Two cases of canine lymphoid leukemia were diagnosed in post mortem exam. The first dog, a male two years old Bull Terrier, with enlarged abdominal volume, diarrhea and jaundice. The second one, a nine years old female Dalmatian, with progressive weight loss and skin lesions for six months. At necropsy, both animals showed generalized lymphadenomegaly and hepatosplenomegaly. Histopathologic exam and immunohistochemical stain, with anti-CD3 and anti-CD79a antibodies, confirmed acute and chronic lymphoid leukemia, respectively, both with T immunophenotype.(AU)

Dois casos de leucemia linfone canina diagnosticados ao exame post mortem / Two canine lymphoid leukemias diagnosed in post mortem exam

Leucemias são neoplasias mesenquimais malignas com origem nas células da medula óssea e/ou sangue e classificadas em mieloide ou linfoide a depender do tipo celular envolvido. Dois casos de leucemia linfoide canina foram diagnosticados ao exame post mortem. O primeiro, um cão, Bull Terrier, de dois anos e histórico de aumento de volume abdominal, diarréia escura e icterícia. O segundo, uma cadela, Dálmata, de nove anos, que apresentava emagrecimento progressivo e lesões cutâneas havia seis meses. Ao exame macroscópico, os dois animais apresentaram linfadenomegalia generalizada e hepatoesplenomegalia. A partir dos exames histopatológico e imunoistoquímico, utilizando os anticorpos anti-CD3 e anti-CD79a, foram confirmados os diagnósticos de leucemia linfóide aguda e crônica, respectivamente, ambas de imunofenótipo T.

Leukemias are malignant mesenchymal neoplasias from the bone marrow cells and/or blood cells and it may be myeloid or limphoyd depending cell type involved. Two cases of canine lymphoid leukemia were diagnosed in post mortem exam. The first dog, a male two years old Bull Terrier, with enlarged abdominal volume, diarrhea and jaundice. The second one, a nine years old female Dalmatian, with progressive weight loss and skin lesions for six months. At necropsy, both animals showed generalized lymphadenomegaly and hepatosplenomegaly. Histopathologic exam and immunohistochemical stain, with anti-CD3 and anti-CD79a antibodies, confirmed acute and chronic lymphoid leukemia, respectively, both with T immunophenotype.