Testicular and kinetics parameters of spermatozoa from ovines fed with growing levels of hay and alfafa (Medicago sativa) / Parâmetros testiculares e de cinética de espermatozoides de ovinos alimentados com níveis crescentes de feno de alfafa (Medicago sativa)

The objective was to evaluate the inclusion of alfalfa hay in diets for growing sheep on testicular parameters and sperm kinetics sheep. Forty non-castrated male sheep were used, with body weight around 26 kg, ± 1.85 and eight months of age, approximately, evaluated for 100 days (it being 44 days for adaptation at management more diets and 56 for fattening) in feedlot. Treatments were four levels of alfalfa hay 0%, 20%, 40% and 60 %, so that was maintained proportion 60% of roughage for all rations. The type of diet offered to sheep did not affect testicular weight and serum testosterone concentration. The kinetics of sperm ejaculated and epididymal semen recovered also not influenced by the experimental diets. The inclusion of alfalfa hay in sheep diet did not change testicular parameters as well as the kinetics of ejaculated sperm and recovered from epididymal sperm tail may be recommended for nutritional management of breeding.(AU)

Objetivou-se avaliar a inclusão de feno de alfafa em rações para ovinos em crescimento sobre parâmetros testiculares e de cinética espermática de ovinos. Utilizaram-se 40 ovinos em crescimento, com peso corporal médio inicial de 26 ± 1,85 kg e oito meses de idade, aproximadamente, avaliados durante 100 dias (sendo 44 dias para adaptação ao manejo e 56 dias para a terminação) em confinamento. Os tratamentos foram compostos por quatro níveis de feno alfafa: 0%, 20%, 40% e 60%, de modo que foi mantida proporção de 60% de volumoso para todas as rações. O tipo de dieta oferecida aos ovinos não influenciou o peso testicular, bem como a concentração sérica de testosterona.  A cinética espermática do ejaculado e do sêmen recuperado do epidídimo também não sofreu influência das dietas experimentais. A inclusão de feno de alfafa na dieta de ovinos não altera parâmetros testiculares, bem como a cinética espermática do ejaculado e também dos espermatozoides recuperados da cauda do epidídimo, podendo ser recomendado em manejo nutricional de reprodutores.(AU)

Testicular and kinetics parameters of spermatozoa from ovines fed with growing levels of hay and alfafa (Medicago sativa) / Parâmetros testiculares e de cinética de espermatozoides de ovinos alimentados com níveis crescentes de feno de alfafa (Medicago sativa)

The objective was to evaluate the inclusion of alfalfa hay in diets for growing sheep on testicular parameters and sperm kinetics sheep. Forty non-castrated male sheep were used, with body weight around 26 kg, ± 1.85 and eight months of age, approximately, evaluated for 100 days (it being 44 days for adaptation at management more diets and 56 for fattening) in feedlot. Treatments were four levels of alfalfa hay 0%, 20%, 40% and 60 %, so that was maintained proportion 60% of roughage for all rations. The type of diet offered to sheep did not affect testicular weight and serum testosterone concentration. The kinetics of sperm ejaculated and epididymal semen recovered also not influenced by the experimental diets. The inclusion of alfalfa hay in sheep diet did not change testicular parameters as well as the kinetics of ejaculated sperm and recovered from epididymal sperm tail may be recommended for nutritional management of breeding.

Objetivou-se avaliar a inclusão de feno de alfafa em rações para ovinos em crescimento sobre parâmetros testiculares e de cinética espermática de ovinos. Utilizaram-se 40 ovinos em crescimento, com peso corporal médio inicial de 26 ± 1,85 kg e oito meses de idade, aproximadamente, avaliados durante 100 dias (sendo 44 dias para adaptação ao manejo e 56 dias para a terminação) em confinamento. Os tratamentos foram compostos por quatro níveis de feno alfafa: 0%, 20%, 40% e 60%, de modo que foi mantida proporção de 60% de volumoso para todas as rações. O tipo de dieta oferecida aos ovinos não influenciou o peso testicular, bem como a concentração sérica de testosterona.  A cinética espermática do ejaculado e do sêmen recuperado do epidídimo também não sofreu influência das dietas experimentais. A inclusão de feno de alfafa na dieta de ovinos não altera parâmetros testiculares, bem como a cinética espermática do ejaculado e também dos espermatozoides recuperados da cauda do epidídimo, podendo ser recomendado em manejo nutricional de reprodutores.

Effect of dietary selenium and vitamin E supplementation on testicular morphology and serum testosterone concentration in goats following scrotal insulation

Background: Heat directly applied to the testis has been providing information regarding the damage triggering mechanisms on spermatogenesis and possible treatments to prevent testicular changes. Testis submitted to heat-shock have inhibition of the local antioxidant defense mechanisms against lipid peroxidation and free radicals. Vitamin E and Selenium protect biological membranes against free radicals to prevent membrane lipid peroxidation. The current assay evaluated the effect of dietary supplementation with Selenium and Vitamin E on testicular parenchyma and testosterone levels of goats submitted to heat shock by scrotal insulation.Materials, Methods & Results: The effect of dietary selenium and vitamin E supplementation on testicular parameters and serum testosterone concentration was evaluated in goats subjected to scrotal insulation. The animals were randomly allocated into two groups (n = 6) to receive either a control diet (CO) or a diet supplemented with selenium and vitamin E (SE). The animals received supplementation for 120 days: 60 days prior to scrotal insulation, 18 days during scrotal insulation and 42 days after scrotal insulation. Orchiectomy was performed on three animals from each group, immediately after the end of scrotal insulation. The remaining animals were neutered at the end of the experimental period (120 days). Testicles were routinely process [...](AU)

Effect of dietary selenium and vitamin E supplementation on testicular morphology and serum testosterone concentration in goats following scrotal insulation

Background: Heat directly applied to the testis has been providing information regarding the damage triggering mechanisms on spermatogenesis and possible treatments to prevent testicular changes. Testis submitted to heat-shock have inhibition of the local antioxidant defense mechanisms against lipid peroxidation and free radicals. Vitamin E and Selenium protect biological membranes against free radicals to prevent membrane lipid peroxidation. The current assay evaluated the effect of dietary supplementation with Selenium and Vitamin E on testicular parenchyma and testosterone levels of goats submitted to heat shock by scrotal insulation.Materials, Methods & Results: The effect of dietary selenium and vitamin E supplementation on testicular parameters and serum testosterone concentration was evaluated in goats subjected to scrotal insulation. The animals were randomly allocated into two groups (n = 6) to receive either a control diet (CO) or a diet supplemented with selenium and vitamin E (SE). The animals received supplementation for 120 days: 60 days prior to scrotal insulation, 18 days during scrotal insulation and 42 days after scrotal insulation. Orchiectomy was performed on three animals from each group, immediately after the end of scrotal insulation. The remaining animals were neutered at the end of the experimental period (120 days). Testicles were routinely process [...]

Efeito da adição de Trolox e Glutationa reduzida na viabilidade in vitro de espermatozoides de cães / Effect of Trolox and Glutathione reduced (GSH) addition on the in vitro viability of dog cryopreserved sperm

Com o objetivo de avaliar o efeito da adição dos antioxidantes Trolox e Glutationa reduzida (GSH) na viabilidade in vitro de espermatozoides criopreservados de cães, amostras de sêmen foram colhidas de animais das raças Buldogue Francês (n=1), Basset Hound (n=2) e Rottweiler (n=1), por meio de manipulação digital. A seguir, procedeu-se à avaliação macroscópica e microscópica e divisão do volume de sêmen em alíquotas, de acordo com os experimentos e grupos experimentais, utilizando-se diferentes concentrações de Trolox (200 e 300 µM/L) ou GSH (2 e 5 µM/L). As amostras foram envasadas em palhetas (0,25 mL), criopreservadas em máquina de congelação e armazenadas em nitrogênio líquido. Após descongelação a 37 ºC (60 seg) e incubação durante 60 minutos, constatou-se que os resultados de motilidade, vigor e espermatozoides com acrossomas íntegros evidenciaram diferença significativa (P<0,05) apenas entre os tempos de incubação, nos três experimentos. No Exp. 2, a adição de 2 e 5 µM/L de GSH (G2 e G3) determinou maior (P<0,05) porcentual de células sem estresse oxidativo. Concluiu-se que GSH, nas concentrações de 2 e 5 µM/L, pode ser adicionada ao diluente de criopreservação do sêmen de cães.

In order to evaluate the effect of adding Trolox and Glutathione reduced antioxidants on the in vitro viability of dog cryopreserved sperm, semen samples were harvested from animals of French Bulldog (n=1), Basset Hound (n=2) and Rottweiler (n=1) breeds, through digital manipulation. After macroscopic and microscopic evaluation, the volume of semen was divided in aliquots, according to the experiments and experimental groups, using different concentrations of Trolox (200 and 300 µM/L) or GSH (2 and 5 µM/L). The samples were packaged in straws (0.25 mL), cryopreserved in freezing machine and stored in liquid nitrogen. After thawing at 37ºC (60 sec) and incubation during 60 minutes, we verified that motility, vigor and sperm with intact acrosomes showed significant difference (P<0.05) only among incubation times, on the three experiments. In Exp. 2, the addition of 2 and 5 µM/L of GSH (G2 and G3) determined higher (P<0.05) percentage of sperm without oxidative stress. Therefore, we could conclude that GSH, on the concentration of 2 and 5 µM/L, can be added to diluent of dog semen cryopreservation.

Efeito da adição de Trolox e Glutationa reduzida na viabilidade in vitro de espermatozoides de cães / Effect of Trolox and Glutathione reduced (GSH) addition on the in vitro viability of dog cryopreserved sperm

Com o objetivo de avaliar o efeito da adição dos antioxidantes Trolox e Glutationa reduzida (GSH) na viabilidade in vitro de espermatozoides criopreservados de cães, amostras de sêmen foram colhidas de animais das raças Buldogue Francês (n=1), Basset Hound (n=2) e Rottweiler (n=1), por meio de manipulação digital. A seguir, procedeu-se à avaliação macroscópica e microscópica e divisão do volume de sêmen em alíquotas, de acordo com os experimentos e grupos experimentais, utilizando-se diferentes concentrações de Trolox (200 e 300 µM/L) ou GSH (2 e 5 µM/L). As amostras foram envasadas em palhetas (0,25 mL), criopreservadas em máquina de congelação e armazenadas em nitrogênio líquido. Após descongelação a 37 ºC (60 seg) e incubação durante 60 minutos, constatou-se que os resultados de motilidade, vigor e espermatozoides com acrossomas íntegros evidenciaram diferença significativa (P<0,05) apenas entre os tempos de incubação, nos três experimentos. No Exp. 2, a adição de 2 e 5 µM/L de GSH (G2 e G3) determinou maior (P<0,05) porcentual de células sem estresse oxidativo. Concluiu-se que GSH, nas concentrações de 2 e 5 µM/L, pode ser adicionada ao diluente de criopreservação do sêmen de cães.(AU)

In order to evaluate the effect of adding Trolox and Glutathione reduced antioxidants on the in vitro viability of dog cryopreserved sperm, semen samples were harvested from animals of French Bulldog (n=1), Basset Hound (n=2) and Rottweiler (n=1) breeds, through digital manipulation. After macroscopic and microscopic evaluation, the volume of semen was divided in aliquots, according to the experiments and experimental groups, using different concentrations of Trolox (200 and 300 µM/L) or GSH (2 and 5 µM/L). The samples were packaged in straws (0.25 mL), cryopreserved in freezing machine and stored in liquid nitrogen. After thawing at 37ºC (60 sec) and incubation during 60 minutes, we verified that motility, vigor and sperm with intact acrosomes showed significant difference (P<0.05) only among incubation times, on the three experiments. In Exp. 2, the addition of 2 and 5 µM/L of GSH (G2 and G3) determined higher (P<0.05) percentage of sperm without oxidative stress. Therefore, we could conclude that GSH, on the concentration of 2 and 5 µM/L, can be added to diluent of dog semen cryopreservation.(AU)