Leiomiossarcoma metastático em ganso africano (Anser cygnoides) / Metastatic leiomyosarcoma in African goose (Anser cygnoides)

Background: In birds, neoplasms are more frequently observed in Psittaciformes and Galliformes and rarely seen in Columbiformes and Anseriformes, with few reports of the occurrence of mesenchymal neoplasms such as leiomyosarcoma affecting birds. Therefore, the objective of this study was to describe a case of metastatic leiomyosarcoma in an African goose (Anser cygnoides), analyzing the epidemiological, clinical and pathological aspects. Case: A 10-month-old male African goose, was referred to the Veterinary Hospital, Universidade Federal de Campina Grande, Patos, Paraiba, Brazil, whit ataxia, tremors of intention in the head, and nystagmus about one month ago, progressing to lateral decubitus. Due to the unfavorable prognosis, animal was euthanized. Samples of the organs of the coelomic cavity and central nervous system were collected for histologic examination. The samples were fixed in 10% buffered formalin. After fixation, the organs were embedded in paraffin, cut into 4-5 µm sections, and then stained with hematoxylin and eosin. Paraffin blocks with liver, kidney and encephalon fragments were selected and sent for immunohistochemical analysis. The primary antibodies used were: alpha-smooth muscle actin (monoclonal 1A4), anti-vimentin (monoclonal, V9), CD57 (monoclonal, NK1) and cytokeratin (monoclonal, AE1/AE3) and incubated for 18 h at 4Cº. As an amplification and detection system polymer and labeled by addition of the liquid diaminobenzidine+substratechromogen system and counterstained with Harris hematoxylin. Macroscopically were observed in the liver nodular multifocal areas yellowish, sometimes coalescing, firm, and elevated to the surface that at the cut deepened to the parenchyma. In the left kidney there was a similar tumor mass. In the left frontal lobe, there was nodular focal area, well circumscribed, yellowish and protruding. To cutting surface it compressed the parietal and temporal lobe and showed surface yellowish and smooth. Microscopically, the liver was diffusely infiltrated by mesenchymal neoplasia, expansive, infiltrative, poorly circumscribed and not encapsulated, constituted by spindle cells arranged in interlaced bundles. The cells were elongated with sparse cytoplasm, slightly eosinophilic and indistinct borders with rounded to elongated nuclei, with coarse chromatin and evident nucleoli. In fragments of kidney and brain, neoplastic infiltration similar to that described in the liver was observed. In immunohistochemistry, neoplastic cells were positive with antibodies anti-vimentin and alpha-smooth muscle actin. Discussion: The diagnosis of metastatic leiomyosarcoma in an African goose was based on epidemiological, clinical and pathological findings and confirmed by immunohistochemistry. Reports of neoplasms in birds are relatively rare, however the occurrence of metastatic leiomyosarcoma affecting goose in the most varied locations has been described, from skin to organs of the coelomic cavity like kidney, ovary and intestinal wall. In this case, there is the unusual occurrence of dissemination in the liver, kidney and cerebral cortex, progressing to a neurological clinic condition. There are rare cases of metastatic leiomyosarcoma in geese African goose (Anser cygnoides). The main differential diagnoses include fibrosarcomas, neurofibrosarcomas and histiocytic sarcomas, which are similar macroscopically and histologically.

Melanose talâmica em caprinos / Thalamic melanosis in goats

Background: Melanosis is a blackened pigmentation resulting from the accumulation of melanocytes in tissues that are not normally pigmented. This change in the color of the organs occurs due to the agglomeration of melanocytes originating from abnormal migration during embryogenesis and does not cause dysfunction to the affected organ. Although melanosis frequently occurs in several species and affects several organs such as the brain and spinal cord leptomeninges, involvement in the thalamus region is unusual. The objective of this work was to report 2 cases of thalamic melanosis in goats, determining the pathological and histochemical aspects that assist in the diagnosis of this condition. Cases: Two cases of thalamic melanosis in goats were diagnosed. In both cases, the animals had no nervous history disease and clinical signs. The cause of death in cases 1 and 2 was established based on anatomopathological findings and clinical signs being diagnosed with mycoplasmosis and asphyxia, respectively. After fixing and making cross-sections of the brain, a focal lengthy blackened area was observed on the thalamus surface in both cases. Microscopically, lesions in the brain were similar in both cases and exclusively affected the thalamus. These cells had abundant cytoplasm, well delimited with brownish granular pigment. The nuclei were difficult to visualize and in some cells, it was rounded, well-defined, morphologically compatible with melanocytes. Melanocytes were mainly distributed around neurons and often distended the perivascular space of multiple blood vessels. In Fontana Masson staining, the granules in the cytoplasm of these cells stained strongly black. The Prussian Blue, Periodic Acid- Schiff's, Von Kossa, and Giemsa stains were negative, and the pigment remained brown. In the unstained slides, assembled after the deparaffinization and clarification process, it was observed the permanence of cells with blackish-brown pigment in the cytoplasm. In immunohistochemistry, strong immunostaining of pigmented cells with the Anti-MelanA antibodies was observed in both cases. Discussion: The diagnosis of thalamic melanosis in goats was carried out based on the characteristic pathological findings, in which melanin pigments were demonstrated and identified through HE, Fontana-Masson staining, and unstained slides and confirmed by the IHC. The use of complementary histochemical techniques was fundamental for the classification of the pigment as melanin, demonstrating to be an accessible and reliable tool for the diagnosis of pathological processes that lead to the accumulation of pigments and or material in the tissues. The occurrence of melanin in the thalamus may be associated with a failure in the migration of melanoblasts, which would go to the optical pathways or to the thalamus. This erratic migration of melanoblasts can be explained by the fact that the forebrain is the embryogenic origin of the optic and diencephalon pathways. Macroscopically, thalamic melanosis must be differentiated mainly from neoplastic processes such as melanoma and hemangiosarcoma, pigmented fungus infections, Phalaris angusta poisoning, listeriosis, neurocutaneous melanosis, and neuromelanin. It was concluded that thalamic melanosis is an uncommon alteration in goats and although it has been diagnosed as an incidental necropsy finding, should be included in the differential diagnosis of diseases that affect the central nervous system, especially those that have a color change associated with the deposition of pigments in the tissues.

Primary gallbladder adenocarcinoma in a cat

Background: Biliary tumors have low incidence in cats and are more common in elderly animals. Hepatobiliary tumors have different classifications and their etiology is difficult to interpret. In most cases, the diagnosis is only possible in advanced stages, which clouds the precursor factors. The late diagnosis is explained by the absence or specificity of clinical manifestations and laboratory changes. The majority of hepatobiliary neoplasms in cats are incidental findings in surgeries or necropsies. This study aimed to report clinical, laboratory, pathological and immunohistochemical results in a feline case of gallbladder adenocarcinoma. Case: 4-year-old male castrated mix breed cat was admitted at a veterinary clinic with a history of polyuria / polydipsia, anorexia, apathy, jaundice and emesis for 60 days. The animal had been treated in another clinic with silymarin, famotidine and cyanocobalamin, and fed by esophageal tube based on the presumptive clinical diagnosis of cholangitis. The clinical examination revealed jaundice, abdominal pain, weight loss, enlargement of the liver and gallbladder and the presence of a structure in the epigastric region. Based on clinical signs, blood tests (complete blood count and liver enzymes), abdominal ultrasound and thoracic radiography were requested. In the exam results, eosinophilia, bilirubinemia and increased alkaline phosphatase, glutamic pyruvic transaminase and gamma glutamyl transferase were observed, in addition to the presence of icteric and hemolyzed serum. Ultrasound exam revealed thickened and dilated cystic and common ducts, large and thick gallbladder, bile with bile mud, hypoechogenic liver, thickening in the duodenal papilla and enlarged pancreatic and duodenal lymph nodes. There were no alterations in thoracic radiography. Based on the findings, the diagnosis of...

Primary gallbladder adenocarcinoma in a cat

Background: Biliary tumors have low incidence in cats and are more common in elderly animals. Hepatobiliary tumors have different classifications and their etiology is difficult to interpret. In most cases, the diagnosis is only possible in advanced stages, which clouds the precursor factors. The late diagnosis is explained by the absence or specificity of clinical manifestations and laboratory changes. The majority of hepatobiliary neoplasms in cats are incidental findings in surgeries or necropsies. This study aimed to report clinical, laboratory, pathological and immunohistochemical results in a feline case of gallbladder adenocarcinoma. Case: 4-year-old male castrated mix breed cat was admitted at a veterinary clinic with a history of polyuria / polydipsia, anorexia, apathy, jaundice and emesis for 60 days. The animal had been treated in another clinic with silymarin, famotidine and cyanocobalamin, and fed by esophageal tube based on the presumptive clinical diagnosis of cholangitis. The clinical examination revealed jaundice, abdominal pain, weight loss, enlargement of the liver and gallbladder and the presence of a structure in the epigastric region. Based on clinical signs, blood tests (complete blood count and liver enzymes), abdominal ultrasound and thoracic radiography were requested. In the exam results, eosinophilia, bilirubinemia and increased alkaline phosphatase, glutamic pyruvic transaminase and gamma glutamyl transferase were observed, in addition to the presence of icteric and hemolyzed serum. Ultrasound exam revealed thickened and dilated cystic and common ducts, large and thick gallbladder, bile with bile mud, hypoechogenic liver, thickening in the duodenal papilla and enlarged pancreatic and duodenal lymph nodes. There were no alterations in thoracic radiography. Based on the findings, the diagnosis of...(AU)

Infecção por Gurltia paralysans em gatos domésticos no Estado de Pernambuco, Brasil / Gurltia paralysans infection in domestic cats in the State of Pernambuco, Brazil

Background: The Gurltia paralysans nematode was initially described in Chile and for many years it was believed that thedisease caused by this parasite was restricted to this country. However, in Argentina, Uruguay and more recently in Brazil,among other countries, cases of Gurltiosis have been described in both domestic and wild cats. This disease is chronic anddebilitating due to the progressive paralysis developed. This study aimed to describe the clinical, epidemiological and pathological aspects of G. paralysans infection in domestic cats of the Agreste region of the state of Pernambuco, Northeast Brazil.Case: Clinical, epidemiological and pathological aspects of G. paralysans infection in domestic cats in the rural area oftwo Agreste municipalities in the state of Pernambuco, Brazil, are described. Seven farms were visited, in which 11 maleand female affected felines were evaluated. Among these, euthanasia was performed in four cases, at the owners requestand due to the advanced stage of the disease. Clinical signs began with ataxia of the pelvic limbs and evolved to jumpingdifficulty, lateral falls, muscle atrophy, pelvic limb scarring, and paralysis at the most severe stage of the disease, whichdeveloped in a one-year period, approximately. According to the owners, the affected cats died between six months andone year after the initial clinical signs. At necropsy, there were segments of the spinal cord with extensive reddish areasin the dura, between T7 and S2, corresponding to varices. These were characterized by numerous congestive, dilatedand tortuous blood vessels observed in the dorsal plane, but more pronounced in the ventral plane of the meninges. Inthe bladder, multifocal areas of hemorrhage were observed. Histologically, vascular lesions in veins and venules of theleptomeninges were characterized by venous varices with thrombosis, fibrosis and intravascular parasites associated withmoderate...(AU)

Abdome agudo em cutias no nordeste do Brasil / Acute abdomen in agouti in Northeastern of Brazil

Este trabalho apresenta pela primeira vez relatos de abdome agudo em cutia (Dasyprocta aguti Linnaeus, 1758) criada em cativeiro no Centro de Multiplicação de Animais Silvestres (CEMAS), Mossoró, Rio Grande do Norte. Sabendo que no cativeiro o estresse, a concentração de organismos patogênicos e erros no manejo alimentar podem acarretar distúrbios gastrintestinais em animais silvestres, torna-se necessário a identificação das causas do óbito em animais criados nesta condição. Dessa forma, foi realizado em cinco cutias adultas, que vieram a óbito após apresentar quadro clínico de distensão e dor abdominal, inquietação como também prostração, o exame anatomopatológico, que consiste no exame externo do animal, abertura das cavidades torácica e abdominal e retirada dos órgãos, seguida de estudo macroscópico. Sendo possível diagnosticar como causa do abdome agudo e consequente óbito, vólvulo intestinal, isquemia intestinal por trombo na artéria mesentérica e obstrução do intestino por impactação alimentar.

This case presents for the first time reports of acute abdomen in red-rumped agouti (Dasyprocta aguti Linnaeus, 1758) raised in captivity at the Wild Animals Multiplication Center (CEMAS), Mossoró, Rio Grande do Norte. Knowing that in captivity stress, concentration of pathogenic organisms and errors in food management can lead to gastrointestinal disorders in wild animals, it is necessary to identify the causes of death in animals raised in this condition. Thus, it was performed in five adult women, who died after clinical presentation of distension and abdominal pain, restlessness as well as prostration, anatomopathological examination, which consists of the external examination of the animal, opening of the thoracic and abdominal cavities and withdrawal of the followed by macroscopic study. It is possible to diagnose as the cause of the acute abdomen and consequent death, intestinal volvulus, intestinal ischemia due to thrombus in the mesenteric artery and intestinal obstruction due to food impaction.

Tríade felina / Feline triad

A tríade felina é uma síndrome que envolve o desenvolvimento concomitante de colangite, pancreatite e doença inflamatória intestinal (DII). Os gatos têm condições anatômicas que os predispõem ao desenvolvimento dessas doenças. O objetivo deste trabalho foi realizar uma revisão da literatura sobre o assunto e relatar um caso de tríade felina. Gata de 14 anos, castrada e sem raça definida, foi atendida em uma clínica particular na cidade de Fortaleza - Ceará. Na ananese, o responsável se queixou dos sinis clinicos de vômito, anorexia, apatia, letargia, ausência de defecação e icterícia. Foi realizada biópsia do fígado, duodeno e pâncreas por laparotomia exploradora. A análise histopatológica desses órgãos revelou a presença de colangite esclerosante crônica acentuada, pancreatite crônica leve e DII crônica. O paciente não resistiu às complicações do quadro clínico e morreu dois dias após a avaliação clínica. Métodos invasivos, como laparotomia exploradora para biópsia incisional, ainda são, apesar do custo e do risco anestésico envolvidos, os meios mais adequados para obter amostras para exame histopatológico e para fazer um diagnóstico definitivo se houver suspeita de tríade felina.

The feline triad is a syndrome that involves the concomitant development of cholangitis, pancreatitis, and inflammatory bowel disease (IBD). Cats have anatomical conditions that pre-dispose them to the development of these diseases. The objective of this work was to perform a literature review on the subject and report a feline triad case. A 14-year-old female cat, spayed and with no defined breed was attended in a private clinic in the city of Fortaleza - Ceará. In the anamnesis, the guardian complained of the symptoms of vomiting, anorexia, apathy, lethargy, absence of defecation and jaundice. It was performed a biopsy of liver, duodenum and pancreas by exploratory laparotomy. The histopathological analysis of these organs revealed the presence of marked chronic sclerosing cholangitis, mild chronic pancreatitis and chronic IBD. The patient did not withstand the complications of the clinical condition and came to death two days after clinic evaluation. Invasive methods such as exploratory laparotomy for incisional biopsy are still, despite the cost and anesthetic risk involved, the most appropriate means to obtain samples for histopathological examination and to make a definitive diagnosis if feline triad is suspected.

Infecção por Gurltia paralysans em gatos domésticos no Estado de Pernambuco, Brasil / Gurltia paralysans infection in domestic cats in the State of Pernambuco, Brazil

Background: The Gurltia paralysans nematode was initially described in Chile and for many years it was believed that thedisease caused by this parasite was restricted to this country. However, in Argentina, Uruguay and more recently in Brazil,among other countries, cases of Gurltiosis have been described in both domestic and wild cats. This disease is chronic anddebilitating due to the progressive paralysis developed. This study aimed to describe the clinical, epidemiological and pathological aspects of G. paralysans infection in domestic cats of the Agreste region of the state of Pernambuco, Northeast Brazil.Case: Clinical, epidemiological and pathological aspects of G. paralysans infection in domestic cats in the rural area oftwo Agreste municipalities in the state of Pernambuco, Brazil, are described. Seven farms were visited, in which 11 maleand female affected felines were evaluated. Among these, euthanasia was performed in four cases, at the owners’ requestand due to the advanced stage of the disease. Clinical signs began with ataxia of the pelvic limbs and evolved to jumpingdifficulty, lateral falls, muscle atrophy, pelvic limb scarring, and paralysis at the most severe stage of the disease, whichdeveloped in a one-year period, approximately. According to the owners, the affected cats died between six months andone year after the initial clinical signs. At necropsy, there were segments of the spinal cord with extensive reddish areasin the dura, between T7 and S2, corresponding to varices. These were characterized by numerous congestive, dilatedand tortuous blood vessels observed in the dorsal plane, but more pronounced in the ventral plane of the meninges. Inthe bladder, multifocal areas of hemorrhage were observed. Histologically, vascular lesions in veins and venules of theleptomeninges were characterized by venous varices with thrombosis, fibrosis and intravascular parasites associated withmoderate...

Tríade felina / Feline triad

A tríade felina é uma síndrome que envolve o desenvolvimento concomitante de colangite, pancreatite e doença inflamatória intestinal (DII). Os gatos têm condições anatômicas que os predispõem ao desenvolvimento dessas doenças. O objetivo deste trabalho foi realizar uma revisão da literatura sobre o assunto e relatar um caso de tríade felina. Gata de 14 anos, castrada e sem raça definida, foi atendida em uma clínica particular na cidade de Fortaleza -Ceará. Na ananese, o responsável se queixou dos sinais clínicos de vômito, anorexia, apatia, letargia, ausência de defecação e icterícia. Foi realizada biópsia do fígado, duodeno e pâncreas por laparotomia exploradora. A análise histopatológica desses órgãos revelou a presença de colangite esclerosante crônica acentuada, pancreatite crônica leve e DII crônica. O paciente não resistiu às complicações do quadro clínico e morreu dois dias após a avaliação clínica. Métodos invasivos, como laparotomia exploradora para biópsia incisional, ainda são, apesar do custo e do risco anestésico envolvidos, os meios mais adequados para obter amostras para exame histopatológico e para fazer um diagnóstico definitivo se houver suspeita de tríade felina.(AU)

The feline triad is a syndrome that involves the concomitant development of cholangitis, pancreatitis, and inflammatory bowel disease (IBD). Cats have anatomical conditions that pre-dispose them to the development of these diseases. The objective of this work was toper form a literature review on the subject and report a feline triad case. A 14-year-old female cat, spayed and with no defined breed was attended in a private clinic in the city of Fortaleza- Ceará. In the anamnesis, the guardian complained of the symptoms of vomiting, anorexia, apathy, lethargy, absence of defecation and jaundice. It was performed a biopsy of liver, duodenum and pancreas by exploratory laparotomy. The histopathological analysis of these organs revealed the presence of marked chronic sclerosing cholangitis, mild chronic pancreatitis and chronic IBD. The patient did not withstand the complications of the clinical condition and came to death two days after clinic evaluation. Invasive methods such as exploratory laparotomy for incisional biopsy are still, despite the cost and anesthetic risk involved, the most appropriate means to obtain samples for histopathological examination and to make a definitive diagnosis if feline triad is suspected.(AU)

Abdome agudo em cutias no nordeste do Brasil / Acute abdomen in agouti in Northeastern of Brazil

Este trabalho apresenta pela primeira vez relatos de abdome agudo em cutia (Dasyprocta aguti Linnaeus, 1758) criada em cativeiro no Centro de Multiplicação de Animais Silvestres (CEMAS), Mossoró, Rio Grande do Norte. Sabendo que no cativeiro o estresse, a concentração de organismos patogênicos e erros no manejo alimentar podem acarretar distúrbios gastrintestinais em animais silvestres, torna-se necessário a identificação das causas do óbito em animais criados nesta condição. Dessa forma, foi realizado em cinco cutias adultas, que vieram a óbito após apresentar quadro clínico de distensão e dor abdominal, inquietação como também prostração, o exame anatomopatológico, que consiste no exame externo do animal, abertura das cavidades torácica e abdominal e retirada dos órgãos, seguida de estudo macroscópico. Sendo possível diagnosticar como causa do abdome agudo e consequente óbito, vólvulo intestinal, isquemia intestinal por trombo na artéria mesentérica e obstrução do intestino por impactação alimentar. (AU)

This case presents for the first time reports of acute abdomen in red-rumped agouti (Dasyprocta aguti Linnaeus, 1758) raised in captivity at the Wild Animals Multiplication Center (CEMAS), Mossoró, Rio Grande do Norte. Knowing that in captivity stress, concentration of pathogenic organisms and errors in food management can lead to gastrointestinal disorders in wild animals, it is necessary to identify the causes of death in animals raised in this condition. Thus, it was performed in five adult women, who died after clinical presentation of distension and abdominal pain, restlessness as well as prostration, anatomopathological examination, which consists of the external examination of the animal, opening of the thoracic and abdominal cavities and withdrawal of the followed by macroscopic study. It is possible to diagnose as the cause of the acute abdomen and consequent death, intestinal volvulus, intestinal ischemia due to thrombus in the mesenteric artery and intestinal obstruction due to food impaction.(AU)

Reproductive toxicity of neem seeds (Azadirachta indica) in male quail (Coturnix coturnix japonica) / Toxicidade reprodutiva da semente de neem (Azadirachta indica ) em codorna macho (Coturnix coturnix japonica)

Considering that neem (Azadirachta indica A. Juss.) causes deleterious effects on fertility, the objective was to evaluate the effect of feeding quail (Coturnix coturnix japonica Linnaeus, 1758) of 90 days old with neem seeds on seminal quality, biometric parameters, and testicular morphometrics, as an experimental model. After the 30 day adaptation period to the experimental conditions, birds were randomly distributed in five treatments of neem seeds incorporated into feed, with 10 replicates: T1 (0%), T2 (5%), T3 (10%), T4 (20%) and T5 (40%). After 60 days of receiving experimental rations, their semen was collected to evaluate the sperm quality, the birds were euthanized, and testes were submitted to biometric, histological, and histomorphometric evaluation. The data were evaluated by analysis of variance and later by Tukey test with a significance level of 5%. The mean values of testicle weight, length, and width significantly decreased in T4 and T5, due degeneration of germinal epithelial cells. In these treatments, it occurred a significant increase (P>0.05) in the average values of the tunica albuginea thickness and a significant reduction in the values of the seminiferous tubule diameter and the height of the germinal epithelium. In addition, the mean values of seminal volume, sperm concentration, sperm motility, vigor, and sperm viability were significantly reduced. Thus, the use of neem in bird feeding, as well as the use for therapeutic purposes, is questionable, considering its negative impact on reproduction.

Considerando que o neem (Azadirachta indica A. Juss.) causa efeitos deletérios na fertilidade, objetivou-se avaliar o efeito da alimentação com sementes de neem sobre a qualidade seminal, parâmetros biométricos e morfometria testicular com codornas (Coturnix coturnix japonica Linnaeus, 1758) com 90 dias de idade, como modelo experimental. Após o período de adaptação de 30 dias às condições experimentais, as aves foram distribuídas aleatoriamente em cinco tratamentos de sementes de neem incorporadas na ração, com 10 repetições: T1 (0%), T2 (5%), T3 (10%), T4 (20%) e T5 (40%). Após 60 dias de receber rações experimentais, seu sêmen foi coletado para avaliar a qualidade espermática, as aves foram sacrificadas e os testículos foram submetidos à avaliação biométrica, histológica e histomorfométrica. Os dados foram avaliados por análise de variância e mais tarde pelo teste de Tukey com um nível de significância de 5%. Os valores médios do peso, comprimento e largura do testículo diminuíram significativamente em T4 e T5, devido à degeneração de células epiteliais germinativas. Nestes tratamentos, ocorreu um aumento significativo (P>0,05) nos valores médios da espessura da túnica albugínea e uma redução significativa nos valores do diâmetro do túbulo seminífero e a altura do epitélio germinal. Além disso, os valores médios do volume seminal, concentração de esperma, motilidade espermática, vigor e viabilidade espermática foram significativamente reduzidos. Assim, o uso de neem na alimentação de aves, bem como o uso para fins terapêuticos, são questionáveis, considerando seu impacto negativo na reprodução.

Reproductive toxicity of neem seeds (Azadirachta indica) in male quail (Coturnix coturnix japonica) / Toxicidade reprodutiva da semente de neem (Azadirachta indica ) em codorna macho (Coturnix coturnix japonica)

Considering that neem (Azadirachta indica A. Juss.) causes deleterious effects on fertility, the objective was to evaluate the effect of feeding quail (Coturnix coturnix japonica Linnaeus, 1758) of 90 days old with neem seeds on seminal quality, biometric parameters, and testicular morphometrics, as an experimental model. After the 30 day adaptation period to the experimental conditions, birds were randomly distributed in five treatments of neem seeds incorporated into feed, with 10 replicates: T1 (0%), T2 (5%), T3 (10%), T4 (20%) and T5 (40%). After 60 days of receiving experimental rations, their semen was collected to evaluate the sperm quality, the birds were euthanized, and testes were submitted to biometric, histological, and histomorphometric evaluation. The data were evaluated by analysis of variance and later by Tukey test with a significance level of 5%. The mean values of testicle weight, length, and width significantly decreased in T4 and T5, due degeneration of germinal epithelial cells. In these treatments, it occurred a significant increase (P>0.05) in the average values of the tunica albuginea thickness and a significant reduction in the values of the seminiferous tubule diameter and the height of the germinal epithelium. In addition, the mean values of seminal volume, sperm concentration, sperm motility, vigor, and sperm viability were significantly reduced. Thus, the use of neem in bird feeding, as well as the use for therapeutic purposes, is questionable, considering its negative impact on reproduction.(AU)

Considerando que o neem (Azadirachta indica A. Juss.) causa efeitos deletérios na fertilidade, objetivou-se avaliar o efeito da alimentação com sementes de neem sobre a qualidade seminal, parâmetros biométricos e morfometria testicular com codornas (Coturnix coturnix japonica Linnaeus, 1758) com 90 dias de idade, como modelo experimental. Após o período de adaptação de 30 dias às condições experimentais, as aves foram distribuídas aleatoriamente em cinco tratamentos de sementes de neem incorporadas na ração, com 10 repetições: T1 (0%), T2 (5%), T3 (10%), T4 (20%) e T5 (40%). Após 60 dias de receber rações experimentais, seu sêmen foi coletado para avaliar a qualidade espermática, as aves foram sacrificadas e os testículos foram submetidos à avaliação biométrica, histológica e histomorfométrica. Os dados foram avaliados por análise de variância e mais tarde pelo teste de Tukey com um nível de significância de 5%. Os valores médios do peso, comprimento e largura do testículo diminuíram significativamente em T4 e T5, devido à degeneração de células epiteliais germinativas. Nestes tratamentos, ocorreu um aumento significativo (P>0,05) nos valores médios da espessura da túnica albugínea e uma redução significativa nos valores do diâmetro do túbulo seminífero e a altura do epitélio germinal. Além disso, os valores médios do volume seminal, concentração de esperma, motilidade espermática, vigor e viabilidade espermática foram significativamente reduzidos. Assim, o uso de neem na alimentação de aves, bem como o uso para fins terapêuticos, são questionáveis, considerando seu impacto negativo na reprodução.(AU)

Sarna psorótica em equino / Psoroptic scabies in equine

This study was carried out on a horse with 3-year-old, male of the Paint horse breed, suffering from psoroptic scabies. The animal presented dermatological symptomatology characterized by areas of alopecia, with edema, intense pruritus and bleeding. The dorsal and face regions was the most affected area of the body. The diagnosis was made based on the animal's anamnesis, epidemiological information, clinical history and skin scrapings, through the microscopic visualization of the adult forms of Psoroptes spp. The therapeutic course was performed through topical and environmental treatment, with proved effective. Psoroptic mange is caused by Psoroptes spp., a mite that can parasitize horses, cattle, sheep and goats. Information available in the literature related to the occurrence of psoroptic scabies in horses is scarce. This case report aims to describe the occurrence of this disease in an equine in the state of Ceará.

Sarna psorótica em equino / Psoroptic scabies in equine

This study was carried out on a horse with 3-year-old, male of the Paint horse breed, suffering from psoroptic scabies. The animal presented dermatological symptomatology characterized by areas of alopecia, with edema, intense pruritus and bleeding. The dorsal and face regions was the most affected area of the body. The diagnosis was made based on the animal's anamnesis, epidemiological information, clinical history and skin scrapings, through the microscopic visualization of the adult forms of Psoroptes spp. The therapeutic course was performed through topical and environmental treatment, with proved effective. Psoroptic mange is caused by Psoroptes spp., a mite that can parasitize horses, cattle, sheep and goats. Information available in the literature related to the occurrence of psoroptic scabies in horses is scarce. This case report aims to describe the occurrence of this disease in an equine in the state of Ceará.(AU)

Achados anatomopatológicos em serpente Boa constrictor vítima de atropelamento / Anatomopathological findings in snake Boa constrictor victim of run over

Acidentes automobilísticos em animais silvestres são cada vez mais comuns. Isso acontece devido ao aparecimento desses animais em áreas urbanas, como consequência da perda de seu habitat natural, que sofre influências do homem, como o desmatamento e o uso econômico dessas áreas. O presente relato descreve o caso de uma jiboia (Boa constrictor) de vida livre, vítima de atropelamento, e seus achados anatomopatológicos decorrentes do acidente. Na necropsia foi observado na região lateral uma laceração na pele de aproximadamente 6 cm de comprimento. Além disso, havia evisceração de segmento de alças intestinais. No subcutâneo e na musculatura evidenciou-se áreas multifocais de hemorragia, e as costelas estavam fraturadas. Ressalta-se a importância da preservação dessas espécies, através de medidas que minimizem as chances de ocorrência de acidentes causados pelo homem, que repercutem na queda da biodiversidade.

Automobile accidents in wild animals are becoming more common. This is due to the appearance of these animals in urban areas as a consequence of the loss of their natural habitat, which is influenced by human, such as deforestation and the economic use of these areas. The present report describes the case of a jiboia (Boa constrictor) victim of trampling and his anatomopathological findings resulting from the accident. At the necropsy a laceration in the skin of approximately 6 cm in length was observed in the lateral region. In addition, there was evisceration of segment of intestinal loops. In the subcutaneous and in the musculature multifocal areas of hemorrhage were evidenced, and the ribs were fractured. The importance of the preservation of these species is emphasized through measures that minimize the chances of human-induced accidents, which affect the decline of biodiversity.

Achados anatomopatológicos em serpente Boa constrictor vítima de atropelamento / Anatomopathological findings in snake Boa constrictor victim of run over

Acidentes automobilísticos em animais silvestres são cada vez mais comuns. Isso acontece devido ao aparecimento desses animais em áreas urbanas, como consequência da perda de seu habitat natural, que sofre influências do homem, como o desmatamento e o uso econômico dessas áreas. O presente relato descreve o caso de uma jiboia (Boa constrictor) de vida livre, vítima de atropelamento, e seus achados anatomopatológicos decorrentes do acidente. Na necropsia foi observado na região lateral uma laceração na pele de aproximadamente 6 cm de comprimento. Além disso, havia evisceração de segmento de alças intestinais. No subcutâneo e na musculatura evidenciou-se áreas multifocais de hemorragia, e as costelas estavam fraturadas. Ressalta-se a importância da preservação dessas espécies, através de medidas que minimizem as chances de ocorrência de acidentes causados pelo homem, que repercutem na queda da biodiversidade.(AU)

Automobile accidents in wild animals are becoming more common. This is due to the appearance of these animals in urban areas as a consequence of the loss of their natural habitat, which is influenced by human, such as deforestation and the economic use of these areas. The present report describes the case of a jiboia (Boa constrictor) victim of trampling and his anatomopathological findings resulting from the accident. At the necropsy a laceration in the skin of approximately 6 cm in length was observed in the lateral region. In addition, there was evisceration of segment of intestinal loops. In the subcutaneous and in the musculature multifocal areas of hemorrhage were evidenced, and the ribs were fractured. The importance of the preservation of these species is emphasized through measures that minimize the chances of human-induced accidents, which affect the decline of biodiversity.(AU)

Colite necrohemorrágica causada por Entamoeba histolytica em um cão / Necro-hemorrhagic colitis caused by Entamoeba histolytica in a dog

Background: Intestinal amebiasis with morphological lesions and clinical manifestations is uncommon in dogs. The disease is caused by the protozoan Entamoeba histolytica, which is commonly observed in its natural hosts, humans and some nonhuman primates. It is occasionally found in the company of animals, usually associated with contact with infected humans. Thus, the objective here is to describe a case of necro-hemorrhagic colitis caused by E. histolytica in a dog infected with the canine distemper virus, in order to characterize the epidemiological and clinicopathological aspects of the disease. Case: An adult, mixed-breed bitch displaying anorexia and ataxia was referred to the veterinary hospital for treatment. Clinical evaluation showed a cachectic animal with 12% dehydration, ocular discharge, and bilateral purulent nasal discharge. A clinical diagnosis of distemper was made, and treatment was instituted. The dog’s signs progressed to walking in circles, aimlessly, with lethargy and blindness. After three days of the onset of neurological signs, the dog developed diarrhea with hematochezia. With no improvement noted, we elected to euthanize the dog. At necropsy, edema was present in subcutaneous tissues, and the lungs had yellow areas in the cranio-ventral portions, which the court was flowing purulent discharge. In the large intestine, segmental distention of the distal portion of the descending colon was observed. The segment was approximately 15 cm in length and consisted of irregular reddish areas. There was also slight thickening of the wall with edematous mucosa containing blood clots, fibrin, and multiple areas of ulceration. Microscopically showed necro-hemorrhagic colitis associated with rounded structures, approximately 15 µm in size, containing abundant eosinophilic cytoplasm that was slightly granular or vacuolated. They also contained nuclei and nucleoli that were central or slightly eccentric […]

Colite necrohemorrágica causada por Entamoeba histolytica em um cão / Necro-hemorrhagic colitis caused by Entamoeba histolytica in a dog

Background: Intestinal amebiasis with morphological lesions and clinical manifestations is uncommon in dogs. The disease is caused by the protozoan Entamoeba histolytica, which is commonly observed in its natural hosts, humans and some nonhuman primates. It is occasionally found in the company of animals, usually associated with contact with infected humans. Thus, the objective here is to describe a case of necro-hemorrhagic colitis caused by E. histolytica in a dog infected with the canine distemper virus, in order to characterize the epidemiological and clinicopathological aspects of the disease. Case: An adult, mixed-breed bitch displaying anorexia and ataxia was referred to the veterinary hospital for treatment. Clinical evaluation showed a cachectic animal with 12% dehydration, ocular discharge, and bilateral purulent nasal discharge. A clinical diagnosis of distemper was made, and treatment was instituted. The dogs signs progressed to walking in circles, aimlessly, with lethargy and blindness. After three days of the onset of neurological signs, the dog developed diarrhea with hematochezia. With no improvement noted, we elected to euthanize the dog. At necropsy, edema was present in subcutaneous tissues, and the lungs had yellow areas in the cranio-ventral portions, which the court was flowing purulent discharge. In the large intestine, segmental distention of the distal portion of the descending colon was observed. The segment was approximately 15 cm in length and consisted of irregular reddish areas. There was also slight thickening of the wall with edematous mucosa containing blood clots, fibrin, and multiple areas of ulceration. Microscopically showed necro-hemorrhagic colitis associated with rounded structures, approximately 15 µm in size, containing abundant eosinophilic cytoplasm that was slightly granular or vacuolated. They also contained nuclei and nucleoli that were central or slightly eccentric […](AU)

Perfuração torácica e fratura de esterno em potro – relato de caso / Thoracic drilling and sternal fracture in foal - case report

The aim of this paper is to describe an attempt to correct a thoracic trauma with drilling and sternum fracture in a filly. Cleaning the thoracic cavity was made, muscle graft placement, reduction and synthesis subcutaneous space of the skin. Recommended antibiotic, anti-inflammatory, analgesic, anti-tetanus serum and rest. Despite the efforts the animal came to death days later because of postsurgical complications.

Typhocolitis by Cyathostomins larvae on a donkey / Tiflocolite por larvas de ciatostomíneos em um asinino

Background: Cyathostomosis is the infection by nematode larvae of small strongyles belonging to the subfamily Cyathostominae (cyathostome). It is primarily a disease of young horses with global distribution. The parasites cause severe injuries to the intestine and the clinically affected animals have severe diarrhea, colic, intussusception, and less frequently intestinal infarction. The aim of this study is to describe the pathological findings of enteritis by cyathostome on a donkey in Brazil. Case: A donkey (Equus asinus), crossbred female mongrel, approximately four-months-old, was referred to the Veterinary Hospital of the Federal University of Campina Grande, Patos, PB, Brazil with a history of trauma. On clinical examination, complete fracture of the left femur was identified. After performing euthanasia the donkey was necropsied. The animal was skinny with pale mucous membranes. The perineal region, base of the tail and hind limbs were dirty with yellowish fecal content. At necropsy, the large intestine had a pasty content and the mucosa was thickened with the multifocal whitish or dark red nodules. The cut surface of these nodules showed caseous material, sometimes associated with larvae in the submucosa. Microscopically, it was observed granulomatous and eosinophilic chronic multifocal associated with intralesional larvae of cyathostome-like nematodes. In the mucosa an [...]