The first report of abnormal age rings in otoliths Lapillus of ariids catfish / Primeiro registro de aneis etários anormais em otólitos Lapillus de bagres arídeos

The present work aimed to record the first presence of abnormal age rings in Cathorops spixii lapillusotoliths from Cananeia-Iguape Estuarine-Lagoon Complex (CIELC), Southern region of Brazilian coast. In August 2018, 59 specimens of C. spixii (Siluriformes, Ariidae) were collected during one station sampling in the northern (n = 25) and another in the southern sector (n = 33) of CIELC. In general, among the otoliths that presented age ring alterations, this divergent zone was observed in opaque and translucent layers, on the right side, between the fifth and seventh age rings.

O presente trabalho teve por objetivo apresentar o primeiro relato de anéis etários anormais nos otólitos lapillus de Cathorops spixii do Complexo estuarino-lagunar de Cananéia-Iguape (CELCI), região sul do litoral brasileiro. Em agosto de 2018, 59 espécimes de C. spixii (Siluriformes, Ariidae) foram coletados durante uma estação de amostragem no setor norte (n = 25) e outra no setor sul (n = 33) do (CELCI). Em geral, entre os otólitos que apresentavam alterações nos anéis de idade, essa zona divergente foi observada em camadas opacas e translúcidas, do lado direito, entre o quinto e o sétimo anéis de idade.

The first report of abnormal age rings in otoliths Lapillus of ariids catfish / Primeiro registro de aneis etários anormais em otólitos Lapillus de bagres arídeos

The present work aimed to record the first presence of abnormal age rings in Cathorops spixii lapillusotoliths from Cananeia-Iguape Estuarine-Lagoon Complex (CIELC), Southern region of Brazilian coast. In August 2018, 59 specimens of C. spixii (Siluriformes, Ariidae) were collected during one station sampling in the northern (n = 25) and another in the southern sector (n = 33) of CIELC. In general, among the otoliths that presented age ring alterations, this divergent zone was observed in opaque and translucent layers, on the right side, between the fifth and seventh age rings.(AU)

O presente trabalho teve por objetivo apresentar o primeiro relato de anéis etários anormais nos otólitos lapillus de Cathorops spixii do Complexo estuarino-lagunar de Cananéia-Iguape (CELCI), região sul do litoral brasileiro. Em agosto de 2018, 59 espécimes de C. spixii (Siluriformes, Ariidae) foram coletados durante uma estação de amostragem no setor norte (n = 25) e outra no setor sul (n = 33) do (CELCI). Em geral, entre os otólitos que apresentavam alterações nos anéis de idade, essa zona divergente foi observada em camadas opacas e translúcidas, do lado direito, entre o quinto e o sétimo anéis de idade.(AU)

The spatial distribution of the subtidal benthic macrofauna and its relationship with environmental factors using geostatistical tools: a case study in Trapandé Bay, southern Brazil

Modeling the distribution patterns of the estuarine macrobenthic community has revealed itself as a difficult task due to spatio-temporal heterogeneity. This study uses ordinary kriging and Poisson modeling to generate distribution maps of the subtidal benthic macrofauna in the Trapandé Bay (southeastern Brazil). Samples were taken in duplicate from 36 locations distributed along nine transects perpendicular to the main estuarine axis in October 2006 and March 2007. One-hundred and seventy taxa belonging to 12 phyla, were identified, with dominance of Annelida Polychaeta. Distribution maps were prepared to illustrate the total density, the number of species and the six most numerous taxa, as well as abiotic parameters. The general distribution pattern has revealed that the greatest number of species and the highest densities are at the estuary mouth, decreasing towards its inner areas. However the temporal and spatial changes observed at the estuary mouth have clearly shown the impact of environmental variations such as nutrients and freshwater input, attributed to increased rainfall in March. The increased flow in the Cananeia Sea, coming from the drainage basin, produces major changes in sediment and faunal composition. Ordinary kriging associated with Poisson modeling has proved to be a powerful and promising tool for modeling the macrofauna, despite the fact that it is not frequently used due to the scarcity of appropriate software.

The spatial distribution of the subtidal benthic macrofauna and its relationship with environmental factors using geostatistical tools: a case study in Trapandé Bay, southern Brazil

Modeling the distribution patterns of the estuarine macrobenthic community has revealed itself as a difficult task due to spatio-temporal heterogeneity. This study uses ordinary kriging and Poisson modeling to generate distribution maps of the subtidal benthic macrofauna in the Trapandé Bay (southeastern Brazil). Samples were taken in duplicate from 36 locations distributed along nine transects perpendicular to the main estuarine axis in October 2006 and March 2007. One-hundred and seventy taxa belonging to 12 phyla, were identified, with dominance of Annelida Polychaeta. Distribution maps were prepared to illustrate the total density, the number of species and the six most numerous taxa, as well as abiotic parameters. The general distribution pattern has revealed that the greatest number of species and the highest densities are at the estuary mouth, decreasing towards its inner areas. However the temporal and spatial changes observed at the estuary mouth have clearly shown the impact of environmental variations such as nutrients and freshwater input, attributed to increased rainfall in March. The increased flow in the Cananeia Sea, coming from the drainage basin, produces major changes in sediment and faunal composition. Ordinary kriging associated with Poisson modeling has proved to be a powerful and promising tool for modeling the macrofauna, despite the fact that it is not frequently used due to the scarcity of appropriate software.

The spatial distribution of the subtidal benthic macrofauna and its relationship with environmental factors using geostatistical tools: a case study in Trapandé Bay, southern Brazil

Modeling the distribution patterns of the estuarine macrobenthic community has revealed itself as a difficult task due to spatio-temporal heterogeneity. This study uses ordinary kriging and Poisson modeling to generate distribution maps of the subtidal benthic macrofauna in the Trapandé Bay (southeastern Brazil). Samples were taken in duplicate from 36 locations distributed along nine transects perpendicular to the main estuarine axis in October 2006 and March 2007. One-hundred and seventy taxa belonging to 12 phyla, were identified, with dominance of Annelida Polychaeta. Distribution maps were prepared to illustrate the total density, the number of species and the six most numerous taxa, as well as abiotic parameters. The general distribution pattern has revealed that the greatest number of species and the highest densities are at the estuary mouth, decreasing towards its inner areas. However the temporal and spatial changes observed at the estuary mouth have clearly shown the impact of environmental variations such as nutrients and freshwater input, attributed to increased rainfall in March. The increased flow in the Cananeia Sea, coming from the drainage basin, produces major changes in sediment and faunal composition. Ordinary kriging associated with Poisson modeling has proved to be a powerful and promising tool for modeling the macrofauna, despite the fact that it is not frequently used due to the scarcity of appropriate software.(AU)

INQUÉRITO SOROLÓGICO DE ANTICORPOS ANTI-Rickettsia, EM CÃES NOS MUNICÍPIOS DE CANANÉIA E ITAPEVA, ESTADO DE SÃO PAULO, BRASIL

A febre maculosa brasileira (FMB) é uma doença infecciosa aguda, causada por bactérias pertencentes ao gênero Rickettsia. Os carrapatos são vetores para a maioria das espécies de Rickettsia. Na América do Sul, ela é transmitida principalmente por carrapatos do complexo Amblyomma cajennense. No Brasil foram relatadas nove espécies de Rickettsia, sendo Amblyomma sculptum o principal vetor de R. rickettsii para humanos. O objetivo deste estudo foi investigar a soroprevalência de R. rickettsii e R. parkeri em cães nos municípios de Cananéia e Itapeva, SP, e sua relação com a fragmentação da vegetação nas proximidades as áreas urbanas. O sangue utilizado nesta pesquisa foi coletado de cães atendidos durante as campanhas de controle populacional de cães e gatos realizadas em ambos os municípios, mediante autorização prévia dos proprietários. Os soros obtidos dessas amostras foram testados para R. rickettsii e R. parkeri através da Reação de Imunofluorescência indireta (RIFI). Foram analisadas um total de 186 amostras de soro provenientes dos dois municípios, sendo 81 amostras de Cananéia e 105 amostras de Itapeva, entre machos e fêmeas. No município de Cananéia, cinco amostras apresentaram reação positiva ao antígeno de R. parkeri, sendo uma delas positiva também para R. rickettsii, porém com um título pelo menos quatro vezes maior para a primeira, o que indica a circulação nessa área de alguma Rickettsia do grupo da febre maculosa com provável reação homóloga a R. parkeri. Já em Itapeva nenhuma das amostras apresentou reação para as riquétsias testadas, sendo consideradas todas negativas. Uma provável explicação para a ocorrência de soropositividade para o antígeno de R. parkeri nos cães de Cananéia, é o estreito contato entre as áreas florestadas e as habitações humanas, o que permite que os animais tenham livre acesso as áreas de mata e se infestem com carrapatos infectados com Rickettsia.

Brazilian Spotted Fever (FMB) is an acute infectious disease caused by bacteria belonging to the genus Rickettsia. Ticks are vectors for most species of Rickettsia. In South America, it is mainly transmitted by ticks from the Amblyomma cajennense complex. In Brazil nine species of Rickettsia were reported, with Amblyomma sculptum being the main vector of R. rickettsii for humans. The objective of this study was to investigate the seroprevalence of R. rickettsii and R. parkeri in dogs in the municipalities of Cananéia and Itapeva, state of São Paulo, and its relation with the fragmentation of vegetation in the proximities of urban areas. The blood used in this research was collected from dogs with previous authorization of the owners, during the campaigns of population control of dogs and cats carried out in both municipalities. The sera obtained from these samples were tested for R. rickettsii and R. parkeri by the indirect immunofluorescence reaction. A total of 186 serum samples from both municipalities were analyzed, being 81 from Cananéia and 105 from Itapeva. In the municipality of Cananéia, five samples showed a positive reaction to the R. parkeri antigen, indicating circulation in this area of some Rickettsia of the spotted fever group with probable reaction homologous to R. parkeri. In Itapeva, none of the samples presented a reaction to the tested Rickettsia, all of which were considered negative. A probable explanation for the occurrence of seropositivity for the R. parkeri antigen in Cananéia dogs is the close contact between forested areas and human habitations, which allows animals to have free access to these areas and to become infested with ticks infected with Rickettsia.

Mercury and methylmercury content, fatty acids profile, and proximate composition of consumed fish in Cananéia, São Paulo, Brazil / Avaliação da concentração de mercúrio total e metilmercúrio e composição de ácidos graxos em pescado consumido em Cananéia, São Paulo, Brasil

Fifty three individuals of four most commonly consumed fish species by the Cananéia city population, in São Paulo, Brazil, were analyzed to determine total mercury (Total Hg) and methylmercury (MeHg) levels, as well as, proximate composition and fatty acid profile. The muscle of three carnivorous species Centropomus parallelus (Fat snook), Macrodon ancylodon (King weakfish) and Micropogonias furnieri (Whitemouth croaker), and one planktivorous species Mugil platanus (Mullet) were analyzed. MeHg and Total Hg determinations were performed by Cold Vapour Atomic Absorption Spectrometry (CV AAS). Fatty acid profile was determined by gas chromatography (GC)whilst AOAC methods were used for proximate analysis. The total Hg results (interval) in wet weight basis were: Whitemouth croaker (114442 g kg-1), Fat snook (15-178 g kg-1), King weakfish (12-100 g kg-1) and Mullet ( 1025 g kg-1), and none of the fish species exceeded the Brazilian legislation limits. MeHg values were below FAO/WHO (2007) recommendations. In nutritional terms, the results for proximate composition for all four fish species proved to be an excellent protein source with very low lipid content as was expected. Fatty acids of all fish species were adequate. Total polyunsaturated fatty acid contents varied from 21.9% (Micropogonias furnieri) to 26.4% (Mugil platanus). For the n-3 family, Macrodon ancylodon pre

Cinquenta e três amostras de músculo de pescados mais consumidos pela população de Cananéia, foram avaliados em relação aos teores de mercúrio total (HgT) e metilmercúrio (MeHg), composição centesimal e de ácidos graxos. Os músculos de três espécies carnívoras Centropomus parallelus (Robalo Peba), Macrodon ancylodon (Pescada) e Micropogonias furnieri (Corvina), e uma espécie planctívora, Mugil platanus (Tainha) foram analisadas. As determinações de Hg total e MeHg foram feitas por espectrometria de absorção atômica com geração de vapor frio (CV AAS). A composição de ácidos graxos foi determinada por cromatografia a gás (GC) e a composição centesimal, de acordo com as metodologias preconizadas pela AOAC. Os intervalos de concentração obtidos para Hg total (peso úmido)foram: Corvina (114-442 g kg-1), Robalo (15-178 g kg-1), Pescada (12-100 g kg-1) e Tainha ( 10-25 g kg-1), não excedendo os limites da legislação brasileira. Os valores de MeHg se encontraram abaixo das recomendações da FAO/WHO(2007). Em termos nutricionais, os resultados de composição centesimal para todas as espécies analisadas provaram ser uma excelente fonte de proteína com conteúdo muito baixo de lipídeos. Os teores de ácidos graxos poliinsaturados totais variaram de 21,9% (Pescada) a 26,4% (Tainha). Para a família n-3,Macrodon ancylodon apresentou o maior valor (20,9%) e Micropogonias furnieri, o menor