A clinical trial and oral wound treated by essential oil of Lippia sidoides mouthrinse in horses

Background: Frequently the equine oral cavity is target of dental alterations, irregular wear of the dentition, tartar accumulation, gums inflammation, and other oral mucosa lesions. Therapeutically, active plants have been proposed to actpreventively against oral infections. The objective of this study was to evaluate a mouthrinse prepared with Lippia sidoidesCham. essential oil in equine dental diseases and oral wound healing treatment.Materials, Methods & Results: The experimental protocol was approved by the Ethics Committee of the Use of Animals(CEUA) of the State University of Ceara (UECE), n° 1158255-0/75. Mouthrinse was prepared with essential oil extractedfrom leaves of L. sidoides (EOLS). Thymol was the main constituent of the oil, up to 70%. Horses were chosen basedon clinical and oral examination. Dental calculus and gingivitis grade from incisor and canine teeth were scored duringevaluation. Two groups were used; OELS group (n = 10), and control group (n = 6), treated with mouthwash without EO(MR). A surgical scar (0.5 x 2 cm) was made near tooth 106 with the animals sedated. The lesions were observed on days 0(D0), 3 (D3), 7 (D7), and 14 (D14) after treatment that was applied once in 48 h for 14 days. Recovery of oral mucosa was,blindly, evaluated in tissue samples. EOLS treatment reduced teeth calculus scores from D0 to D14 (P ≤ 0.05). In relationto histological parameters, EOLS treatment induced discreet proliferation of fibroblasts and a moderated inflammation onD3; moderated proliferation of fibroblasts and moderated inflammation, intense angiogenesis and re-epithelization on D7,and intense fibroblast proliferation, intense angiogenesis, and reduced inflammation with complete...(AU)

A clinical trial and oral wound treated by essential oil of Lippia sidoides mouthrinse in horses

Background: Frequently the equine oral cavity is target of dental alterations, irregular wear of the dentition, tartar accumulation, gums inflammation, and other oral mucosa lesions. Therapeutically, active plants have been proposed to actpreventively against oral infections. The objective of this study was to evaluate a mouthrinse prepared with Lippia sidoidesCham. essential oil in equine dental diseases and oral wound healing treatment.Materials, Methods & Results: The experimental protocol was approved by the Ethics Committee of the Use of Animals(CEUA) of the State University of Ceara (UECE), n° 1158255-0/75. Mouthrinse was prepared with essential oil extractedfrom leaves of L. sidoides (EOLS). Thymol was the main constituent of the oil, up to 70%. Horses were chosen basedon clinical and oral examination. Dental calculus and gingivitis grade from incisor and canine teeth were scored duringevaluation. Two groups were used; OELS group (n = 10), and control group (n = 6), treated with mouthwash without EO(MR). A surgical scar (0.5 x 2 cm) was made near tooth 106 with the animals sedated. The lesions were observed on days 0(D0), 3 (D3), 7 (D7), and 14 (D14) after treatment that was applied once in 48 h for 14 days. Recovery of oral mucosa was,blindly, evaluated in tissue samples. EOLS treatment reduced teeth calculus scores from D0 to D14 (P ≤ 0.05). In relationto histological parameters, EOLS treatment induced discreet proliferation of fibroblasts and a moderated inflammation onD3; moderated proliferation of fibroblasts and moderated inflammation, intense angiogenesis and re-epithelization on D7,and intense fibroblast proliferation, intense angiogenesis, and reduced inflammation with complete...

Antimicrobial efficacy of Curcuma zedoaria extract as assessed by linear regression compared with commercial mouthrinses

The antimicrobial activity of Curcuma zedoaria (Christm) Roscoe extract against some oral microorganisms was compared with the antimicrobial activity of five commercial mouthrinses in order to evaluate the potential of the plant extract to be incorporated into formulas for improving or creating antiseptic activity. The in vitro antimicrobial efficacy of plant extracts and commercial products were evaluated against Streptococcus mutans, Enterococcus faecalis, Staphylococcus aureus and Candida albicans using a linear regression method to evaluate the microbial reduction obtained in function of the exposure time, considering as effectiveness a 99.999% reduction in count of standardized microbial populations within 60 seconds. The results showed that the antimicrobial efficacy of Curcuma zedoaria (Christm) Roscoe extract was similar to that of commercial products, and its incorporation into a mouthrinse could be an alternative for improving the antimicrobial efficacy of the oral product.

A atividade antimicrobiana do extrato de Curcuma zedoaria (Christm) Roscoe contra algumas bactérias da microbiota bucal foi comparada com a atividade antimicrobiana de cinco anti-sépticos comerciais, a fim de avaliar o potencial do extrato vegetal de ser incorporado em formulações com a finalidade de melhorar ou conferir atividade anti-séptica. A eficácia antimicrobiana in vitro do extrato vegetal e produtos comerciais foi avaliada frente a Streptococcus mutans,Enterococcus faecalis,Staphylococcus aureus e Candida albicans, utilizando o método de regressão linear para avaliar a redução microbiana obtida em função do tempo de exposição, considerando como eficácia a redução de 99,999% na contagem de população microbiana padronizada em 60 segundos. Os resultados demonstraram que a eficácia antimicrobiana do extrato de Curcuma zedoaria (Christm) Roscoe foi similar a de produtos comerciais e que sua incorporação em anti-sépticos bucais pode ser uma alternativa para aumentar a eficácia antimicrobiana de produtos para uso oral.