Hepatoprotective effect of Zinc and Magnesium against subchronicCadmium toxicity on biochemical, histopathological andneurobehavioral parameters in Wistar rats

The aim of this study was carried out to investigate the possible hepato protective effect of Zinc and Magnesium against subchronic cadmium toxicity on biochemical, histopathological and neurobehavioral parameters in Wistar rats during 90 days. Fourty Wistar rats, were randomly divided in four experimental group: 1, control only water; 2, water and oral administration (Cd); 3, (Cd+Mg) in water oral administration; 4, (Cd+Zn) in water oral administration. Zinc and Magnesium was used to reduce hepatoxicity and neurobehavioral disorders induced by Cadmium in Wistar rats. The Cd-Zn and Cd-Mg groups received 10 mg/kg of ZnCl2, 10 mg/kg of MgCl2 added to drinking water. The Cd group received 15mg / kg of CdCl2. Blood glucose level and body weight were measured every day. According to the results, which have been obtained. There was a reduction in body weight gain due to Cd group and an increase liver weight. The results indicated also changes in biochemical parameters, which are characterized by an increase in serum glucose, serum urea, serum creatinine, of animals contaminated with cadmium compared to the control animals. However, the supplementation of (Zn) and Mg combination improved some but not all the previous parameters. Moreover, the findings showed variation in hematological parameters of Cd treated animals. In other word, there was a rise in number of white cells, and reduction of red cells counts, hemoglobin concentration and hematocrit percentage, but all these parameters returned approximately to their normal values after combined Zn and Mg addition. The neurobehavioral test shows an increase in the number of cells crossed, the number of straightening in the test. Open field in the group exposed to Cd resulting in locomotors hyperactivity compared to the control and it is minimal in other groups treated with Zn and Mg...(AU)

Hepatoprotective effect of Zinc and Magnesium against subchronicCadmium toxicity on biochemical, histopathological andneurobehavioral parameters in Wistar rats

The aim of this study was carried out to investigate the possible hepato protective effect of Zinc and Magnesium against subchronic cadmium toxicity on biochemical, histopathological and neurobehavioral parameters in Wistar rats during 90 days. Fourty Wistar rats, were randomly divided in four experimental group: 1, control only water; 2, water and oral administration (Cd); 3, (Cd+Mg) in water oral administration; 4, (Cd+Zn) in water oral administration. Zinc and Magnesium was used to reduce hepatoxicity and neurobehavioral disorders induced by Cadmium in Wistar rats. The Cd-Zn and Cd-Mg groups received 10 mg/kg of ZnCl2, 10 mg/kg of MgCl2 added to drinking water. The Cd group received 15mg / kg of CdCl2. Blood glucose level and body weight were measured every day. According to the results, which have been obtained. There was a reduction in body weight gain due to Cd group and an increase liver weight. The results indicated also changes in biochemical parameters, which are characterized by an increase in serum glucose, serum urea, serum creatinine, of animals contaminated with cadmium compared to the control animals. However, the supplementation of (Zn) and Mg combination improved some but not all the previous parameters. Moreover, the findings showed variation in hematological parameters of Cd treated animals. In other word, there was a rise in number of white cells, and reduction of red cells counts, hemoglobin concentration and hematocrit percentage, but all these parameters returned approximately to their normal values after combined Zn and Mg addition. The neurobehavioral test shows an increase in the number of cells crossed, the number of straightening in the test. Open field in the group exposed to Cd resulting in locomotors hyperactivity compared to the control and it is minimal in other groups treated with Zn and Mg...

Estudo histopatológico dos órgãos reprodutivos de ratos tratados com extrato etanólico de Simarouba versicolor em ensaio de toxicidade sub-crônica / Histopathological study of the reproductive organs of rats treated with ethanol extract of Simarouba versicolor in test sub-chronic toxicity

The aim of this study was to evaluate the reproductive organs on the effect of Simarouba versicolorethanol extract (EEOH-Sv) at a dose of 10mg / kg administered for 60 days / VO. The testes, epididymis,prostate and seminal vesicles were sectioned and placed in vials Boan solution for 24 hours and then preservedin alcohol at 70%. Histopathology of organs were dehydrated in xylol, impregnated and embedded in paraffin.Subsequently cut in the microtome at a thickness of 3 m and subjected to hematoxylin-eosin. The results of thehistopathology of the testis was observed that the EEOH-Sv caused concretions in 100% of animals evaluatedand 20% of the animals showed sharp associated with degeneration of the seminiferous tubules. In theepididymis was observed in 40% epithelial thickening, and there was 100% of benign prostatic hypertrophyassociated in 20% of animals inflammatory infiltrate. Thus, it is concluded that-Sv EEOH was toxic to thisorgans except the seminal vesicle.(AU)

Estudo histopatológico dos órgãos reprodutivos de ratos tratados com extrato etanólico de Simarouba versicolor em ensaio de toxicidade sub-crônica / Histopathological study of the reproductive organs of rats treated with ethanol extract of Simarouba versicolor in test sub-chronic toxicity

The aim of this study was to evaluate the reproductive organs on the effect of Simarouba versicolorethanol extract (EEOH-Sv) at a dose of 10mg / kg administered for 60 days / VO. The testes, epididymis,prostate and seminal vesicles were sectioned and placed in vials Boan solution for 24 hours and then preservedin alcohol at 70%. Histopathology of organs were dehydrated in xylol, impregnated and embedded in paraffin.Subsequently cut in the microtome at a thickness of 3 m and subjected to hematoxylin-eosin. The results of thehistopathology of the testis was observed that the EEOH-Sv caused concretions in 100% of animals evaluatedand 20% of the animals showed sharp associated with degeneration of the seminiferous tubules. In theepididymis was observed in 40% epithelial thickening, and there was 100% of benign prostatic hypertrophyassociated in 20% of animals inflammatory infiltrate. Thus, it is concluded that-Sv EEOH was toxic to thisorgans except the seminal vesicle.

EFEITO NEFROPROTETOR DA AÇÃO DE PROBIÓTICOS EM RATOS INTOXICADOS POR DICROMATO DE POTÁSSIO

Objetivou-se avaliar o efeito de probiótico na função renal de ratos Wistar intoxicados por dicromato de potássio (K2Cr2O7). Utilizou-se 80 ratos Wistar, machos com 21 a 25 dias, divididos aleatoriamente em dois tratamentos (n=40 ratos/tratamento). No tratamento DK os animais consumiram 0, 12, 24 e 36 mg de K2Cr2O7 adicionado na dieta e o tratamento DK+P os ratos consumiram 0, 12, 24 e 36 mg de K2Cr2O7 na dieta com 0,2% de probiótico. Esses animais consumiram suas respectivas dietas durante 90 dias, foram eutanasiados por exsanguinação e colhidas amostras de sangue para realização de dosagem sérica de creatinina e uréia e realizou-se nos rins análise histopatológico. A creatinina sérica do tratamento DK+P foi significativamente (p<0,05) menor em relação ao tratamento DK em todas as doses de dicromato estudadas. A uréia sérica não diferiu significativamente (p>0,05) entre os tratamentos DK e DK+P. Não houve diferença significativa (p>0,05) na concentração sérica de creatinina e uréia dos ratos que consumiram 0 e 12 mg de dicromato e probiótico, mas dos demais animais a creatinina e uréia sérica aumentou significativamente (p<0,05) juntamente com as doses crescentes de dicromato estudadas em ambos os tratamentos experimentais. Os ratos dos tratamentos DK e DK+P apresentaram degeneração hidrópica nos túbulos renais em todas as doses estudadas de K2Cr2O7. Os ratos do tratamento DK apresentaram nefrite intersticial. Conclui-se que a suplementação com probiótico foi benéfica para a filtração glomerular nos ratos intoxicados com baixa dose de dicromato de potássio, mas não evitou a degeneração hidrópica tubular nos ratos Wistar intoxicados pelo dicromato de potássio.

The objective was to evaluate the effect of probiotic on renal function in intoxicated Wistar rats by potassium dichromate (K2Cr2O7). We used 80 male Wistar rats, that were 21 to 25 days old, randomly divided into two treatments (n = 40 rats/treatment). In the DK treatment the animals consumed 0, 12, 24 and 36 mg of K2Cr2O7 added to the diet and in the DK+P treatment the rats consumed 0, 12, 24 and 36 mg of K2Cr2O7 in the diet with 0.2% probiotic. Those animals consumed their respective diets for 90 days, then, they were euthanized by exsanguination and blood samples were taken to evaluate serum creatinine and urea levels and histopathological analysis of the kidneys were realized. Serum creatinine levels in the DK+P treatment was significantly (p<0.05) lower in relation to the DK treatment in all dichromate doses analyzed. Serum urea levels did not differ significantly (p>0.05) between the DK and DK+P treatments. There was no significant difference (p>0.05) in serum creatinine and urea concentrations in the rats that consumed 0 and 12 mg of dichromate and probiotic, but in the other animals serum creatinine and urea increased significantly (p<0.05) along with increasing doses of the dichromate in both experimental treatments. The rats in DK and DK+P treatments showed hydropic degeneration in renal tubules at all doses of K2Cr2O7. The DK treatment rats showed interstitial nephritis. It was concluded that supplementation with the probiotic was beneficial for glomerular filtration in rats intoxicated with low doses of potassium dichromate, but did not prevent the tubular hydropic degeneration in Wistar rats intoxicated by potassium dichromate.