Resumo
Purpose: To evaluate the morphometric, macroscopic and microscopic aspects of experimentally induced partial-thickness burns in rats treated with different silver-based dressings. Methods: Wistar rats were used, divided into six treatments: saline (NaCl 0.9%); silver sulfadiazine 1%; Silvercel; Mepilex Ag; Aquacel Ag and Acticoat. The animals were monitored daily and euthanized at 7, 14 and 30 days after injury induction (DAI). Results: At 7 DAI, necrosis/crust was greater in control, silver sulfadiazine and Mepilex Ag treatments, granulation tissue was induced by Aquacel Ag, polymorphonuclear infiltrate (PMN) infiltration was intensified by Mepilex Ag; mononuclear infiltrate (MN) infiltration and angiogenesis were increased by Silvercel. At 14 DAI, hemorrhage was decreased by Silvercel and Mepilex Ag, PMN infiltration increased by Acticoat. At 30 DAI, angiogenesis was greater in the Acticoat treatment and fibroblasts were increased by Acticoat and Mepilex Ag. Collagen was induced at 14 DAI by silver sulfadiazine and Aquacel Ag and, at 30 DAI, by silver sulfadiazine and Silvercel treatments. Conclusions: Silvercel and Acticoat presented better results than the other products. However, all the dressings were better than the control at some point during the process, and may contribute to the healing of partial thickness burns. Silvercel and Aquacel Ag treatments induced better cosmetic outcomes regarding wound closure and scarring.
Assuntos
Animais , Ratos , Sulfadiazina de Prata/uso terapêutico , Queimaduras Químicas/terapia , Compostos de Prata/uso terapêutico , Curativos Hidrocoloides/veterinária , Ratos WistarResumo
Background: Thermal injury is one of the most common type of skin damage found in clinical care, which results in achronic wound. The treatment of chronic wounds should be specific, because it has a greater vulnerability of the organismto the external agents input. So, the treatment can be performed with covers to keep the lesion clean and free of contamination and also promote healing. Considering the amount of products available for the treatment, as well as the scarcityof studies in the literature proving the effectiveness of hydrocolloid in dogs, the aim of this case report was to describe athird degree burn wound in the sternal region of a dog treated with this material.Case: A 2-year-old non-castraded German Spitz dog, was attended in a private veterinary clinic located in Ribeirão Pretocity, São Paulo state. The animal was submitted to general anesthesia for microneedling session to treat alopecia X,and after the procedure, it showed a burn focus due to the resting time on the thermal mattress, affecting the entire sternalregion. The choice treatment was a surgical debridement followed by second intention healing. The superficial crust of thewound was removed and the debridement of almost all the tissue that was not fit for healing was continued, resulting in awide wound, with healthy tissue and only a small portion of devitalized musculature. On the same day, the daily dressingphase with crystal sugar was started, in order to promote the debridement of the remaining devitalized tissue and enablethe appearance of granulation tissue, together with the asepsis provided by the chlorhexidine ointment (0.7 g/100mL).After five days, the wound presented with ascending granulation, with no signs of contamination and minimal residue ofdevitalized tissue. Then, the treatment with the hydrocolloid plate was chosen, which was cut and molded to the wound...
Assuntos
Animais , Cães , Cicatrização , Curativos Hidrocoloides/veterinária , Queimaduras/terapia , Queimaduras/veterinária , Bandagens/veterináriaResumo
Background: Thermal injury is one of the most common type of skin damage found in clinical care, which results in achronic wound. The treatment of chronic wounds should be specific, because it has a greater vulnerability of the organismto the external agents input. So, the treatment can be performed with covers to keep the lesion clean and free of contamination and also promote healing. Considering the amount of products available for the treatment, as well as the scarcityof studies in the literature proving the effectiveness of hydrocolloid in dogs, the aim of this case report was to describe athird degree burn wound in the sternal region of a dog treated with this material.Case: A 2-year-old non-castraded German Spitz dog, was attended in a private veterinary clinic located in Ribeirão Pretocity, São Paulo state. The animal was submitted to general anesthesia for microneedling session to treat alopecia X,and after the procedure, it showed a burn focus due to the resting time on the thermal mattress, affecting the entire sternalregion. The choice treatment was a surgical debridement followed by second intention healing. The superficial crust of thewound was removed and the debridement of almost all the tissue that was not fit for healing was continued, resulting in awide wound, with healthy tissue and only a small portion of devitalized musculature. On the same day, the daily dressingphase with crystal sugar was started, in order to promote the debridement of the remaining devitalized tissue and enablethe appearance of granulation tissue, together with the asepsis provided by the chlorhexidine ointment (0.7 g/100mL).After five days, the wound presented with ascending granulation, with no signs of contamination and minimal residue ofdevitalized tissue. Then, the treatment with the hydrocolloid plate was chosen, which was cut and molded to the wound...(AU)
Assuntos
Animais , Cães , Curativos Hidrocoloides/veterinária , Queimaduras/terapia , Queimaduras/veterinária , Cicatrização , Bandagens/veterináriaResumo
Background: Fibrosarcomas are malignant neoplasms originating from fibroblasts that are normally located in the skin, subcutaneous tissue and adjacent to bones. The main treatment for fibrosarcoma is surgery. The removal of large neoplasms from dogs limbs usually leads to the formation of large skin defects, often preventing primary occlusion and thus requiring reconstructive surgery to close the wound. This paper reports on the surgical procedure and postoperative complications pursuant to the excision of a fibrosarcoma in the right forelimb and reconstruction with a full-thickness autologous graft in a dog. Case: A 12-year-old Brazilian mastiff presenting a nodule located in the distal segment of the right forelimb, which had grown over a 2-year period, was referred for clinical evaluation. Blood and imaging tests were performed, which showed no changes and excluded the possibility of metastasis. The cytological examination was suggestive of a mesenchymal neoplasm. The patient underwent tumor excision surgery followed by reconstructive surgery with a full-thickness autologous graft to close the defect. The neoplasm was excised by means of a circular geometric figure incision using a 3 cm margin. The defect resulting from removal of the tumor was kept constantly moistened and wrapped in surgical compresses until grafting was performed. The graft was harvested from the region of the right flank based on a surgical field mold taken from the recipient bed. After harvesting the graft, all the subcutaneous tissue was removed until the hair follicles were visible. Fenestrations were then made throughout the entire graft and it was placed in the recipient bed and...
Assuntos
Animais , Cães , Curativos Hidrocoloides/veterinária , Extremidade Superior/cirurgia , Fibrossarcoma/cirurgia , Fibrossarcoma/veterinária , Transplante Autólogo/veterinária , Procedimentos de Cirurgia Plástica/veterináriaResumo
Background: Fibrosarcomas are malignant neoplasms originating from fibroblasts that are normally located in the skin, subcutaneous tissue and adjacent to bones. The main treatment for fibrosarcoma is surgery. The removal of large neoplasms from dogs limbs usually leads to the formation of large skin defects, often preventing primary occlusion and thus requiring reconstructive surgery to close the wound. This paper reports on the surgical procedure and postoperative complications pursuant to the excision of a fibrosarcoma in the right forelimb and reconstruction with a full-thickness autologous graft in a dog. Case: A 12-year-old Brazilian mastiff presenting a nodule located in the distal segment of the right forelimb, which had grown over a 2-year period, was referred for clinical evaluation. Blood and imaging tests were performed, which showed no changes and excluded the possibility of metastasis. The cytological examination was suggestive of a mesenchymal neoplasm. The patient underwent tumor excision surgery followed by reconstructive surgery with a full-thickness autologous graft to close the defect. The neoplasm was excised by means of a circular geometric figure incision using a 3 cm margin. The defect resulting from removal of the tumor was kept constantly moistened and wrapped in surgical compresses until grafting was performed. The graft was harvested from the region of the right flank based on a surgical field mold taken from the recipient bed. After harvesting the graft, all the subcutaneous tissue was removed until the hair follicles were visible. Fenestrations were then made throughout the entire graft and it was placed in the recipient bed and...(AU)
Assuntos
Animais , Cães , Fibrossarcoma/cirurgia , Fibrossarcoma/veterinária , Extremidade Superior/cirurgia , Transplante Autólogo/veterinária , Curativos Hidrocoloides/veterinária , Procedimentos de Cirurgia Plástica/veterináriaResumo
This study aimed to macroscopically and microscopically evaluate the healing of skin wounds induced in rats by topical application of cassava polyamide biopolymer hydrogel. In total, 32 rats were used and divided into four groups (n= 8): negative control - saline solution; positive control - use of commercial ointment; experimental group - I - ointment + cassava hydrogel; experimental group - II - cassava hydrogel. A 1cm2 wound induced on the animals dorsum was treated and evaluated. At day 21 post-operation, the animals were sacrificed by anesthetic overdose, and then 1cm2 of cicatricial skin from the wound region was collected. The material was cut to evaluate healing. In the macroscopic evaluation, complete healing was observed at the end of 21 days. Re-epithelialization was observed histologically; the connective tissue in the negative control, positive, and experimental - I groups was characterized by an abundance of collagen fibers, fibroblasts, and blood vessels. In experimental group - II additional healing was observed, as evidenced by the arrangement of collagen fibers and fibroblasts, and the reduction of neoformed vessels. Thus, we concluded that the hydrogel can assist in healing skin wounds, especially in the remodeling phase.(AU)
O objetivo deste estudo foi avaliar macro e microscopicamente a cicatrização de feridas cutâneas induzidas em ratos, a partir da aplicação tópica do hidrogel de biopolímero de poliamido de mandioca. Trinta e dois ratos foram divididos em quatro grupos (n= 8): controle negativo, tratado com solução salina; controle positivo, com pomada comercial; grupo experimental - I, com pomada + hidrogel de mandioca; grupo experimental - II, com hidrogel de mandioca. Feridas induzidas de 1cm 2 no dorso dos animais foram tratadas e avaliadas em intervalos de três a quatro dias. No 21º dia do pós-operatório, os animais foram mortos por aprofundamento anestésico, em seguida foi coletado 1cm 2 de pele da região cicatricial. O material foi cortado, corado pelas técnicas de hematoxilina-eosina e azocarmine-G, para avaliação da cicatrização. Na avaliação macroscópica, foi observada cicatrização completa no final do período de 21 dias. Histologicamente, observou-se reepitelização, o tecido conjuntivo no grupos controle negativo, positivo e experimental - I se caracterizou pela abundância de fibras colágenas, fibroblastos e vasos sanguíneos. No grupo experimental - II, a cicatrização sugere avanço de etapas, evidenciado pelo arranjo das fibras colágenas, pela redução de fibroblastos e dos vasos neoformados. Assim, foi possível concluir que o hidrogel de biopolímero de amido de mandioca pode auxiliar na cicatrização de feridas cutâneas, principalmente na fase de remodelação.(AU)
Assuntos
Animais , Masculino , Ratos , Cicatrização , Ferimentos e Lesões/veterinária , Curativos Hidrocoloides/veterinária , Amidos e FéculasResumo
This study aimed to macroscopically and microscopically evaluate the healing of skin wounds induced in rats by topical application of cassava polyamide biopolymer hydrogel. In total, 32 rats were used and divided into four groups (n= 8): negative control - saline solution; positive control - use of commercial ointment; experimental group - I - ointment + cassava hydrogel; experimental group - II - cassava hydrogel. A 1cm2 wound induced on the animals dorsum was treated and evaluated. At day 21 post-operation, the animals were sacrificed by anesthetic overdose, and then 1cm2 of cicatricial skin from the wound region was collected. The material was cut to evaluate healing. In the macroscopic evaluation, complete healing was observed at the end of 21 days. Re-epithelialization was observed histologically; the connective tissue in the negative control, positive, and experimental - I groups was characterized by an abundance of collagen fibers, fibroblasts, and blood vessels. In experimental group - II additional healing was observed, as evidenced by the arrangement of collagen fibers and fibroblasts, and the reduction of neoformed vessels. Thus, we concluded that the hydrogel can assist in healing skin wounds, especially in the remodeling phase.(AU)
O objetivo deste estudo foi avaliar macro e microscopicamente a cicatrização de feridas cutâneas induzidas em ratos, a partir da aplicação tópica do hidrogel de biopolímero de poliamido de mandioca. Trinta e dois ratos foram divididos em quatro grupos (n= 8): controle negativo, tratado com solução salina; controle positivo, com pomada comercial; grupo experimental - I, com pomada + hidrogel de mandioca; grupo experimental - II, com hidrogel de mandioca. Feridas induzidas de 1cm 2 no dorso dos animais foram tratadas e avaliadas em intervalos de três a quatro dias. No 21º dia do pós-operatório, os animais foram mortos por aprofundamento anestésico, em seguida foi coletado 1cm 2 de pele da região cicatricial. O material foi cortado, corado pelas técnicas de hematoxilina-eosina e azocarmine-G, para avaliação da cicatrização. Na avaliação macroscópica, foi observada cicatrização completa no final do período de 21 dias. Histologicamente, observou-se reepitelização, o tecido conjuntivo no grupos controle negativo, positivo e experimental - I se caracterizou pela abundância de fibras colágenas, fibroblastos e vasos sanguíneos. No grupo experimental - II, a cicatrização sugere avanço de etapas, evidenciado pelo arranjo das fibras colágenas, pela redução de fibroblastos e dos vasos neoformados. Assim, foi possível concluir que o hidrogel de biopolímero de amido de mandioca pode auxiliar na cicatrização de feridas cutâneas, principalmente na fase de remodelação.(AU)
Assuntos
Animais , Masculino , Ratos , Cicatrização , Ferimentos e Lesões/veterinária , Curativos Hidrocoloides/veterinária , Amidos e FéculasResumo
Infecções cutâneas obtidas por cortes nas mãos de manipuladores podem gerar contaminação nos alimentos. O objetivo deste trabalho foi incorporar o bacteriófago UFV-AREG1 em hidrogel de álcool poli(vinílico) PVA, aplicando-o futuramente como curativo adesivo. Foi adicionado 1,0 mL de solução do fago no PVA e seco em estufa por 48 h. Após, os hidrogeis foram submetidos a testes de Intumescimento, Espectroscopia no infravermelho (FTIR) e efeito antimicrobiano sobre E. coli O157:H7. O intumescimento do PVA-fago foi maior que o PVA-controle (p<0,05). A área de inibição do PVA-fago foi de 3,715 cm2 contra 2,916 cm2 do controle. As análises do FTIR mostraram um pico para o PVA-fago não encontrado no PVA-controle. Foi possível incorporar o bacteriófago em hidrogel de PVA e avaliar sua liberação para posterior aplicação como curativo adesivo.(AU)
Assuntos
Humanos , Álcool de Polivinil , Curativos Hidrocoloides , Hidrogéis , Bacteriófagos , Escherichia coli/virologia , Anti-Infecciosos/análise , Infecção dos Ferimentos/prevenção & controleResumo
Background: Shearing wounds on the limbs of dogs usually affect the regions distally from the radiocarpal joint at thethoracic limb and from the tibiotarsal joint at the pelvic limb. The tissue coverage and re-epithelialization of the injuredregion are important factors that should be considered in the definitive surgical treatment. The hydrocolloid membranepromotes selective autolytic debridement and accelerates the formation of granulation tissue and epithelialization. Thepresent study aims to describe the treatment with hydrocolloid membrane dressing of three cases of shearing wounds withconcomitant orthopedic injuries in the pelvic limbs of dogs.Case: Three dogs with pelvic limb injuries after vehicular trauma were selected for treatment. Two patients had shearinglesions on the medial aspect of the pelvic limb with exposure of the tibia and fibula, the talus and the tibiotarsal joint,associated with bone loss on the medial surface of the limb and rupture of the medial collateral ligament of the tarsus.Another patient had a shearing wound on the dorsal surface of the distal region of the pelvic limb, with injury of the digitalextensor tendons and bone exposure of the second and third metatarsals. Initially, the surgical debridement of the lesionwas performed and during the first five days after trauma the wound was cleansed with chlorhexidine solution and topicalapplication of crystallized sugar daily. In this initial period a dry adherent dressing was used on the lesions, without bandagesfor immobilization of the pelvic limb. In all dogs, the hydrocolloid membrane was applied from the sixth day after initialwound management. Immediately after the application of the hydrocolloid membrane, temporary immobilization of theaffected pelvic limb with a padded Robert Jones bandage was performed. The first changes of the hydrocolloid membraneswere performed after five days of their use. Subsequently, the membranes changes...
Assuntos
Animais , Cães , Cicatrização , Curativos Hidrocoloides/veterinária , Ferimentos e Lesões/cirurgia , Ferimentos e Lesões/veterinária , Pelve/cirurgia , Pelve/lesões , Ligamento Colateral UlnarResumo
Background: Shearing wounds on the limbs of dogs usually affect the regions distally from the radiocarpal joint at thethoracic limb and from the tibiotarsal joint at the pelvic limb. The tissue coverage and re-epithelialization of the injuredregion are important factors that should be considered in the definitive surgical treatment. The hydrocolloid membranepromotes selective autolytic debridement and accelerates the formation of granulation tissue and epithelialization. Thepresent study aims to describe the treatment with hydrocolloid membrane dressing of three cases of shearing wounds withconcomitant orthopedic injuries in the pelvic limbs of dogs.Case: Three dogs with pelvic limb injuries after vehicular trauma were selected for treatment. Two patients had shearinglesions on the medial aspect of the pelvic limb with exposure of the tibia and fibula, the talus and the tibiotarsal joint,associated with bone loss on the medial surface of the limb and rupture of the medial collateral ligament of the tarsus.Another patient had a shearing wound on the dorsal surface of the distal region of the pelvic limb, with injury of the digitalextensor tendons and bone exposure of the second and third metatarsals. Initially, the surgical debridement of the lesionwas performed and during the first five days after trauma the wound was cleansed with chlorhexidine solution and topicalapplication of crystallized sugar daily. In this initial period a dry adherent dressing was used on the lesions, without bandagesfor immobilization of the pelvic limb. In all dogs, the hydrocolloid membrane was applied from the sixth day after initialwound management. Immediately after the application of the hydrocolloid membrane, temporary immobilization of theaffected pelvic limb with a padded Robert Jones bandage was performed. The first changes of the hydrocolloid membraneswere performed after five days of their use. Subsequently, the membranes changes...(AU)
Assuntos
Animais , Cães , Ferimentos e Lesões/cirurgia , Ferimentos e Lesões/veterinária , Curativos Hidrocoloides/veterinária , Pelve/lesões , Pelve/cirurgia , Cicatrização , Ligamento Colateral UlnarResumo
Infecções cutâneas obtidas por cortes nas mãos de manipuladores podem gerar contaminação nos alimentos. O objetivo deste trabalho foi incorporar o bacteriófago UFV-AREG1 em hidrogel de álcool poli(vinílico) PVA, aplicando-o futuramente como curativo adesivo. Foi adicionado 1,0 mL de solução do fago no PVA e seco em estufa por 48 h. Após, os hidrogeis foram submetidos a testes de Intumescimento, Espectroscopia no infravermelho (FTIR) e efeito antimicrobiano sobre E. coli O157:H7. O intumescimento do PVA-fago foi maior que o PVA-controle (p<0,05). A área de inibição do PVA-fago foi de 3,715 cm2 contra 2,916 cm2 do controle. As análises do FTIR mostraram um pico para o PVA-fago não encontrado no PVA-controle. Foi possível incorporar o bacteriófago em hidrogel de PVA e avaliar sua liberação para posterior aplicação como curativo adesivo.
Assuntos
Humanos , Bacteriófagos , Curativos Hidrocoloides , Escherichia coli/virologia , Hidrogéis , Álcool de Polivinil , Anti-Infecciosos/análise , Infecção dos Ferimentos/prevenção & controleResumo
To evaluate wound healing in rats by using low-level laser therapy (LLLT) associated with hydrocolloid occlusive dressing . METHODS: Forty male, adult, Wistar rats were used, distributed into four groups: LG (received 2 J/cm² of laser therapy); HG (treated with hydrocolloid); LHG (treated with 2 J/cm² of laser therapy and hydrocolloid); and the CG (treated with 1 mL of 0.9% saline). The wound was evaluated at pre-determined periods 3rd and 7th days, considering the macroscopic and histological parameters (inflammatory cells, capillary neoformation, fibroblasts, collagen formation and reepithelialization). RESULTS: The LG group at seven days showed increased collagen formation, the LHG group at 3 days showed mild collagen formation. The HG group and the CG at 7 days showed complete reepithelialization. CONCLUSION: Low-level laser therapy as well as the hydrocolloid dressing have favored the wound-healing process in rats.(AU)