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1.
Ciênc. rural (Online) ; 53(5): e20210525, 2023. tab
Artigo em Inglês | LILACS-Express | VETINDEX | ID: biblio-1394271

Resumo

ABSTRACT: This study was conducted to evaluate the effects of dietary supplementation with biocomplexed selenium and chromium on the fatty acid profile of the breast and thigh of males and females broilers of the Label Rouge lineage. The experimental design was completely randomized arranged in a 3 x 2 factorial scheme corresponding to three diets (control; 0.40 ppm of chromium; 0.50 ppm of selenium) and two sexes. Each treatment consisted of three plots of five broilers. The fatty acid profile was evaluated in the breast and thigh samples. The addition of biocomplexed selenium in the broilers diets increased the content of long chain fatty acid of the omega 3 and 6 series (docosahexaenoic - DHA and arachidonic acids, respectively), the total of omega 6 and the stearic acid content on the breast (pectoralis major muscle). In addition, selenium supplementation caused a reduction in oleic acid content and in total of monounsaturated in the same muscle. Males had higher contents of linoleic and linolenic acids, and total of polyunsaturated; and lower content of oleic acid, total of monounsaturated and saturated in the thigh. The broilers supplemented with biocomplexed selenium and males presented better lipid quality in the muscle by breast and thigh, respectively.


RESUMO: Este estudo foi conduzido com o objetivo de avaliar os efeitos da suplementação dietética com selênio e cromo biocomplexados sobre o perfil de ácidos graxos do peito e da coxa de frangos de corte machos e fêmeas da linhagem Label Rouge. O delineamento experimental foi inteiramente casualizado em esquema fatorial 3 x 2 correspondendo a três dietas (controle; 0,40 ppm de cromo; 0,50 ppm de selênio) e dois sexos. Cada tratamento foi composto por três parcelas de cinco frangos de corte. O perfil de ácidos graxos foi avaliado nas amostras de peito e coxa. A adição de selênio biocomplexado na dieta de frangos de corte aumentou o teor de ácidos graxos de cadeia longa das séries ômega 3 e 6 (ácido docosahexaenóico - DHA e araquidônico, respectivamente), o total de ômega 6 e o conteúdo de ácido esteárico no peito (músculo pectoralis major). Além disso, a suplementação com selênio causou redução no teor de ácido oleico e no total de monoinsaturados no mesmo músculo. Os machos apresentaram maiores teores dos ácidos linoléico e linolênico e total de poliinsaturados; e menor conteúdo de ácido oleico, total de monoinsaturados e saturados na coxa. Os frangos suplementados com selênio biocomplexado e os machos apresentaram melhor qualidade lipídica no músculo do peito e coxa, respectivamente.

2.
Rev. bras. zootec ; 52: e20220071, 2023. tab
Artigo em Inglês | VETINDEX | ID: biblio-1436806

Resumo

In this study, we evaluated how guanidinoacetic acid (GAA) addition in diets with various metabolizable energy (ME) contents affects the performance of broiler chickens. We also estimated the equivalence of GAA in ME. We distributed 1,280 one-day-old broilers in a completely randomized design with eight treatments, eight replicates, and twenty birds per experimental unit. Treatments were based on ME levels (2,775-2,875-2,975 kcal/kg; 2,850-2,950-3,050 kcal/kg; 2,925-3,025-3,125 kcal/kg; or 3,000-3,100-3,200 kcal/kg, from 1 to 7, 8 to 21, and 22 to 42 days of age) and the inclusion of GAA (0 or 600 mg/kg). Supplementation of GAA increased weight gain in broilers at an energy level of 2,908 kcal/kg and improved feed conversion ratio (FCR) at energy levels of 2,908 and 2,983 kcal/kg. There was a linear reduction in feed intake and an improvement in FCR of broilers with increasing levels of energy in diets, with and without GAA addition. Solving the equivalence equation, by applying each of the weighted average energy levels studied. indicates the GAA equivalence of 133, 103, 74, and 44 kcal/kg of diet. In conclusion, GAA supplementation improves broilers' efficiency of energy use; the average ME equivalence of 600 mg/kg of GAA is 88.5 kcal/kg.


Assuntos
Animais , Glycine max , Galinhas , Zea mays , Dieta/veterinária
3.
Ciênc. rural (Online) ; 53(4): e20210831, 2023. tab
Artigo em Inglês | LILACS-Express | VETINDEX | ID: biblio-1384592

Resumo

ABSTRACT: This study evaluated the effect of including different doses of phytase in broiler diets with different levels of phytate and reduced levels of calcium (Ca) and available phosphorus (aP), on broilers performance, digestibility (CAID), and bone characteristics. One thousand four hundred and four Cobb®500 broilers were used, distributed in a complete randomized design, and a 2x3 factorial arrangement, with two phytate levels (4.25 kg/ton - MP and 6.0 kg/ton - HP) and three phytase levels (0, 750, and 1500 FTU/kg) totalizing six treatments with nine replicates each. The animals that received phytase in their diet showed higher average feed intake and weight gain, and higher bone weight, % mineral residue, % Ca, and % P, when compared to the treatment without phytase (P < 0.01). Animals that received a diet containing HP with the inclusion of 1500FTU/kg phytase had the best CAID of nutrients (P < 0.0001). Regarding phytate, animals that received the MP diet showed a higher CAID of dry matter, and higher %P in the tibia when compared to animals that received the HP diet. The addition of phytase to diets with reduced Ca and aP levels can improve the CAIDs and bone characteristics in the presence of high phytate levels. However, reducing Ca and aP levels in the diet without adding phytase impairs the performance of broilers and has no effect on their carcass and cuts yield.


RESUMO: Este estudo teve como objetivo avaliar o efeito da inclusão de diferentes doses de fitase em rações para frangos de corte com diferentes níveis de fitato e níveis reduzidos de cálcio (Ca) e fósforo disponível (Pd) sobre seu desempenho. Foram utilizados 1.404 frangos machos Cobb®500, distribuídos em delineamento inteiramente casualizado, em esquema fatorial 2x3, com dois níveis de fitato (4,25 kg / ton - MP e 6,0 kg / ton - HP) e três níveis de fitase (0, 750 e 1500 FTU / kg), com nove repetições por tratamento. Foram avaliados os parâmetros de desempenho, e aos 21 dias de idade, quatro frangos por repetição foram abatidos para coleta do conteúdo ileal para análise do coeficiente de digestibilidade ileal aparente (CDIA), e foram removidas a tíbia direita de um frango por repetição para quantificar seu peso e porcentagem de resíduo mineral (% RM), Ca e P. Aos 42 dias de idade, uma ave por repetição foi abatida para medir o rendimento de carcaça e cortes. Os animais que receberam fitase na dieta apresentaram maior consumo médio de ração e ganho de peso médio, e maior peso ósseo, % RM, % Ca e % P, quando comparados ao tratamento sem fitase (P < 0,01). Os animais que receberam dieta contendo HP com inclusão de 1500FTU / kg de fitase apresentaram os melhores CDIAs de nutrientes (P < 0,0001). Em relação ao fitato, os animais que receberam a dieta MP apresentaram maior CDIA de matéria seca e maior %P na tíbia quando comparados aos animais que receberam a dieta HP. A adição de fitase a dietas com níveis reduzidos de Ca e Pd pode melhorar os CDIAs e as características ósseas na presença de níveis elevados de fitato. No entanto, a redução dos níveis de Ca e Pd na dieta sem adição de fitase prejudica o desempenho dos frangos de corte e não afeta o rendimento de carcaças e cortes.

4.
Rev. bras. ciênc. avic ; 25(2): eRBCA-2022-1736, 2023. tab
Artigo em Inglês | VETINDEX | ID: biblio-1427964

Resumo

The objective of the current study was to examine the effect of replacing maize with new or stored wheat on the nutrient intake, digestibility, digesta viscosity, and growth performance of growing broilers from 1-21 days of age. For this purpose, 560 one-day-old male broiler chicks were randomly divided into seven experimental treatments, each with 8 replicates of 10 birds. The control diet was a corn soybean-based diet, while six other experimental treatments replaced the corn of the control diet with either 50% or 100% of new, 1.5 year, and 2.5-year-old wheat. The results showed that the replacement of corn with new wheat at 50% and 100% in the diet of broilers significantly reduced feed intake and body weight gain, and increased FCR (p<0.05) at week 3. Similarly, in the overall period of the starter phase (1-21 days), replacement of corn with new wheat at 50% and 100% in the diet of broilers significantly reduced feed intake, nutrient digestibility, and body weight gain, and increased digesta viscosity and FCR (p<0.05). Based on the findings of the current study, it is concluded that stored wheat successfully replaces corn and new wheat in the diet of broilers. Furthermore, stored wheat has a positive impact on feed intake, performance parameter, digesta viscosity, and nutrient digestibility as compared to new wheat.(AU)


Assuntos
Animais , Masculino , Galinhas/fisiologia , Ingestão de Alimentos/fisiologia , Triticum/fisiologia
5.
Braz. j. biol ; 832023.
Artigo em Inglês | LILACS-Express | LILACS, VETINDEX | ID: biblio-1469182

Resumo

Abstract The current study aimed to determine the effects of different levels of Zingiber officinale as a herbal feed additive on growth performance, carcass characteristic, serum biochemistry, total bacterial count (TBC), gut morphology, and immunological parameters of broilers. A total of 1500, day-old broiler chicks (Hubbard) were equally accredited to five treatment groups, each with six replicates (50 birds/replicate). Five experimental diets were prepared using basal diet i.e. with antibiotics positive control (PC), 3 g/kg ginger (group A), 6 g/kg ginger (group B), 9 g/kg ginger (group C) and without antibiotics negative control (NC). Group A and C showed significantly (p 0.05) higher feed intake (FI) as compared to other groups. Group C showed significantly (p 0.05) lower Total bacterial count (TBC) followed by group B as compared to NC. Carcass characteristics showed non-significant effects among different treatments. Mean villi length and width were significantly (p 0.05) higher in all ginger supplemented groups as compared to the control groups. Blood serum parameters including cholesterol, triglycerides, and low-density lipoproteins (LDL) were significantly (p 0.05) lower in groups B and C in comparison with the control groups. Whereas high-density lipoproteins (HDL) was significantly higher in group B as compared to the others. In conclusion, ginger supplementation @0.6% in the basal diet significantly improved growth performance and gut morphometry of broilers. It also showed a positive impact on cholesterol, triglycerides and gut microbes. Therefore, ginger could be a better substitute for antibiotic growth promoters.


Resumo O presente estudo teve como objetivo determinar os efeitos de diferentes níveis de Zingiber officinale como aditivo à base de plantas medicinais sobre o desempenho de crescimento, características da carcaça, bioquímica sérica, contagem bacteriana total (CBT), morfologia intestinal e parâmetros imunológicos de frangos de corte. Um total de 1.500 pintos de corte de um dia de idade (Hubbard) foram igualmente credenciados em cinco grupos de tratamento, cada um com seis repetições (50 aves/repetição). Cinco dietas experimentais foram preparadas usando dieta basal, ou seja, com controle positivo de antibióticos (PC), 3 g/kg de gengibre (grupo A), 6 g/kg de gengibre (grupo B), 9 g/kg de gengibre (grupo C) e sem controle negativo de antibióticos (NC). Os grupos A e C apresentaram consumo de ração (FI) significativamente (p 0,05) maior do que os outros grupos. O grupo C apresentou contagem bacteriana total (CBT) significativamente menor (p 0,05) seguido pelo grupo B em comparação com o NC. As características da carcaça apresentaram efeitos não significativos entre os diferentes tratamentos. O comprimento e largura médios das vilosidades foram significativamente (p 0,05) maiores em todos os grupos suplementados com gengibre em comparação com os grupos de controle. Os parâmetros séricos do sangue, incluindo colesterol, triglicerídeos e lipoproteínas de baixa densidade (LDL), foram significativamente (p 0,05) menores nos grupos B e C em comparação com os grupos controle. Enquanto as lipoproteínas de alta densidade (HDL) foram significativamente maiores no grupo B em comparação com os outros. Em conclusão, a suplementação de gengibre a 0,6% na dieta basal melhorou significativamente o desempenho de crescimento e a morfometria intestinal de frangos de corte. Ele também mostrou um impacto positivo sobre o colesterol, triglicerídeos e micróbios intestinais. Portanto, o gengibre pode ser um substituto melhor para os promotores de crescimento com antibióticos.

6.
Braz. j. biol ; 832023.
Artigo em Inglês | LILACS-Express | LILACS, VETINDEX | ID: biblio-1469190

Resumo

Abstract The supplementation of Selenium-enriched probiotics is effective in reducing oxidative stress and maintaining meat quality stability in broiler chicken especially under heat stress. An experimental study was conducted to perform Comparative analysis of Selenium yeast with inorganic Se in broilers under heat stress. A total of 120 broilers chicks of one day were assigned to 4 groups each consisting 30 chicks fed on same basal diet but different selenium sources. The basal diet of group D1 was not supplemented with Se source (Negative control), group D2 basal diet was supplemented with inorganic selenium (Sodium selenite 0.22mg/Kg starter phase and 0.15mg/Kg finisher phase), group D3 basal diet was supplemented with commercially available organic selenium (Seleno-methionine 0.22mg/Kg starter phase and 0.15mg/Kg finisher phase) and group D4 basal diet was supplemented with self-developed organic selenium (Se-enriched yeast 0.22mg/Kg starter phase and 0.15mg/Kg finisher phase). The performance parameters i.e. feed intake (FI), live body weight (BW) and FCR were not significantly (p>0.05) effected by selenium supplementation in the starter phase but were significantly (p 0.05) effected in the finisher phase. Selenium supplementation significantly (p 0.05) effected serum Se level in different supplemented groups. Higher serum Se value (58.20±0.06) was recorded in D4 group. Similarly significantly lower selenium value was recorded for D4 and higher was recorded for D1 (11.36±0.08). However lower serum Paraoxonase (PON) value was recorded for D4 (13.24±0.01) and higher for D1 (13.33±0.03). Comparatively self-developed Se enriched yeast increased the Se accumulation and improved antioxidant system. Glutathione peroxidase (GPx) was found higher in D4 (12.333±0.03) followed by D3, D2 and D1 respectively. Whereas superoxide dismutase (SOD) was significantly lower (p 0.05) in D4 (0.1437±0.003) followed by D3 (0.1457±0.002). Selenium supplementation increased the birds survival rate. Birds fed on Se enriched yeast showed higher Se deposition and better antioxidant capacity as compared to other sources of selenium. Se-enriched yeast displayed an improved result on Se deposition in tissues, and oxidative capacity, meat tenderness and immune response level as compared to other sources of selenium.


Resumo A suplementação de probióticos enriquecidos com selênio é eficaz na redução do estresse oxidativo e na manutenção da estabilidade da qualidade da carne em frangos de corte, especialmente sob estresse por calor. Um estudo experimental foi conduzido para realizar uma análise comparativa da levedura selênio com o Se inorgânico em frangos de corte sob estresse térmico. Um total de 120 pintos de um dia foi dividido em 4 grupos, cada um consistindo de 30 pintos alimentados com a mesma dieta basal, mas com diferentes fontes de selênio. A dieta basal do grupo D1 não foi suplementada com fonte de Se (controle negativo), a dieta basal do grupo D2 foi suplementada com selênio inorgânico (selenito de sódio 0,22 mg / kg fase inicial e 0,15 mg / kg fase finalizadora), a dieta basal do grupo D3 foi suplementada com selênio orgânico disponível comercialmente (fase inicial de seleno-metionina 0,22 mg / kg e fase finalizadora de 0,15 mg / kg) e a dieta basal do grupo D4 foi suplementada com selênio orgânico autodesenvolvido (fermento enriquecido com Se 0,22 mg / kg fase inicial e 0,15 mg / kg fase finalizadora). Os parâmetros de desempenho, ou seja, consumo de ração (FI), peso corporal vivo (PC) e FCR não foram significativamente (p > 0,05) afetados pela suplementação de selênio na fase inicial, mas foram significativamente (p 0,05) afetados na fase final. A suplementação de selênio afetou significativamente (p 0,05) o nível de Se sérico em diferentes grupos suplementados. O maior valor de Se sérico (58,20 ± 0,06) foi registrado no grupo D4. Da mesma forma, valor de selênio significativamente menor foi registrado para D4 e maior foi registrado para D1 (11,36 ± 0,08). No entanto, um valor mais baixo de Paraoxonase (PON) sérica foi registrado para D4 (13,24 ± 0,01) e mais alto para D1 (13,33 ± 0,03). A levedura enriquecida com Se comparativamente autodesenvolvida aumentou o acúmulo de Se e melhorou o sistema antioxidante. A glutationa peroxidase (GPx) foi encontrada maior em D4 (12,333 ± 0,03) seguido por D3, D2 e D1 respectivamente. Enquanto a superóxido dismutase (SOD) foi significativamente menor (p 0,05) em D4 (0,1437 ± 0,003) seguido por D3 (0,1457 ± 0,002). A suplementação de selênio aumentou a taxa de sobrevivência da ave. Aves alimentadas com levedura enriquecida com Se apresentaram maior deposição de Se e melhor capacidade antioxidante em comparação com outras fontes de selênio. A levedura enriquecida com Se apresentou um resultado melhorado na deposição de Se nos tecidos, capacidade oxidativa, maciez da carne e nível de resposta imune em comparação com outras fontes de selênio.

7.
Rev. bras. ciênc. avic ; 25(3): eRBCA-2022-1741, 2023. tab, graf, ilus
Artigo em Inglês | VETINDEX | ID: biblio-1451864

Resumo

To effectively develop and utilize high-quality Tianfu broilers, this study evaluated the morphological and structural characteristics of the immune organs of such broilers with different strains (HS1 and HS2) at different developmental stages and analyzed the distribution of mast cells by toluidine blue staining. Moreover, the localization and expression of immunoglobulin, complement C3, C4 and CD3 in immune organs were also detected. The results showed that although there was no significant difference in the development of immune organs in the HS1 and HS2, the number of lymphatic follicles and capsule thickness in the spleen and bursa of Fabricius in HS1 were greater than those in HS2. Additionally, the number of mast cells in the spleen of HS1 was greater at Day 1 and Day 21 and was significantly higher than that of HS2 (p<0.05); the number of mast cells in the bursa of Fabricius reached 9.17 on Day 7, which was significantly higher than that of HS2 (p<0.05). Moreover, the serum IgA and IgM levels in HS1 were higher than those in HS2 on Day 14 and 21 (p<0.05). In addition, the complement C3 content in HS1 was significantly or extremely significantly higher than that in HS2 on Days 1, 14 and 21 (p<0.01, p<0.05), respectively, but significantly lower than in HS2 on Day 7 (p<0.05). These results indicated that the disease resistance of the HS1 line was stronger than that of the HS2 line, which lays a foundation for future disease- resistance breeding of Tianfu broilers.(AU)


Assuntos
Galinhas/imunologia , Sistema Imunitário , Mastócitos/imunologia , Imunoglobulinas/análise
8.
Ciênc. rural (Online) ; 53(4): 1-9, 2023. tab
Artigo em Inglês | VETINDEX | ID: biblio-1412801

Resumo

This study evaluated the effect of including different doses of phytase in broiler diets with different levels of phytate and reduced levels of calcium (Ca) and available phosphorus (aP), on broilers performance, digestibility (CAID), and bone characteristics. One thousand four hundred and four Cobb®500 broilers were used, distributed in a complete randomized design, and a 2x3 factorial arrangement, with two phytate levels (4.25 kg/ton - MP and 6.0 kg/ton - HP) and three phytase levels (0, 750, and 1500 FTU/kg) totalizing six treatments with nine replicates each. The animals that received phytase in their diet showed higher average feed intake and weight gain, and higher bone weight, % mineral residue, % Ca, and % P, when compared to the treatment without phytase (P < 0.01). Animals that received a diet containing HP with the inclusion of 1500FTU/kg phytase had the best CAID of nutrients (P < 0.0001). Regarding phytate, animals that received the MP diet showed a higher CAID of dry matter, and higher %P in the tibia when compared to animals that received the HP diet. The addition of phytase to diets with reduced Ca and aP levels can improve the CAIDs and bone characteristics in the presence of high phytate levels. However, reducing Ca and aP levels in the diet without adding phytase impairs the performance of broilers and has no effect on their carcass and cuts yield.


Este estudo teve como objetivo avaliar o efeito da inclusão de diferentes doses de fitase em rações para frangos de corte com diferentes níveis de fitato e níveis reduzidos de cálcio (Ca) e fósforo disponível (Pd) sobre seu desempenho. Foram utilizados 1.404 frangos machos Cobb®500, distribuídos em delineamento inteiramente casualizado, em esquema fatorial 2x3, com dois níveis de fitato (4,25 kg / ton - MP e 6,0 kg / ton - HP) e três níveis de fitase (0, 750 e 1500 FTU / kg), com nove repetições por tratamento. Foram avaliados os parâmetros de desempenho, e aos 21 dias de idade, quatro frangos por repetição foram abatidos para coleta do conteúdo ileal para análise do coeficiente de digestibilidade ileal aparente (CDIA), e foram removidas a tíbia direita de um frango por repetição para quantificar seu peso e porcentagem de resíduo mineral (% RM), Ca e P. Aos 42 dias de idade, uma ave por repetição foi abatida para medir o rendimento de carcaça e cortes. Os animais que receberam fitase na dieta apresentaram maior consumo médio de ração e ganho de peso médio, e maior peso ósseo, % RM, % Ca e % P, quando comparados ao tratamento sem fitase (P < 0,01). Os animais que receberam dieta contendo HP com inclusão de 1500FTU / kg de fitase apresentaram os melhores CDIAs de nutrientes (P < 0,0001). Em relação ao fitato, os animais que receberam a dieta MP apresentaram maior CDIA de matéria seca e maior %P na tíbia quando comparados aos animais que receberam a dieta HP. A adição de fitase a dietas com níveis reduzidos de Ca e Pd pode melhorar os CDIAs e as características ósseas na presença de níveis elevados de fitato. No entanto, a redução dos níveis de Ca e Pd na dieta sem adição de fitase prejudica o desempenho dos frangos de corte e não afeta o rendimento de carcaças e cortes.


Assuntos
Animais , Aves Domésticas , Dieta , Ração Animal
9.
Acta sci., Anim. sci ; 45: e58738, 2023. tab
Artigo em Inglês | VETINDEX | ID: biblio-1418493

Resumo

An experiment was conducted to evaluate the effect of diets with reduced energy level content, supplemented with carbohydrase, on broiler performance and the coefficient of metabolizability of nutrients. A total of 720 one-day-old male Cobb-500 chicks were distributed in a completely randomized design, with six treatments, eight repetitions of 15 birds each. The treatments were: (1) a positive control, basal diet to meet the requirements of broiler chickens (PC); (2) a negative control, basal diet with a reduction of 80 kcal kg-1 (NC); (3) NC + alphagalactosydase; (4) NC + xylanase; (5) NC + xylanase and alphagalactosydase, and (6) NC + enzymatic blend (alphagalactosydase, xylanase, pectinase and amylase). The nutrient digestibility was not improved by use of enzymes. At 7 days of age, the broilers which were fed diets supplemented with enzymes showed a lower feed intake (FI) and better feed conversion ratio (FCR) than the broilers fed on PC. Both the NC and enzymatic blend resulted in a worse performance of the broilers at 21, 35 and 42 days old. The use of alphagalactosydase and xylanase, isolated or in combination, in a corn-soybean meal-based diet is effective in improving the growth performance of broilers fed energy-reduced diets.(AU)


Assuntos
Animais , Polissacarídeos/análise , Galinhas/fisiologia , Ração Animal/análise , Glycine max , Carboidratos , Zea mays
10.
Rev. bras. ciênc. avic ; 25(3): eRBCA-2023-1801, 2023. tab
Artigo em Inglês | VETINDEX | ID: biblio-1451872

Resumo

This experiment was conducted for 42 days, and aimed to investigate the effect of conditioning temperature and time on feed quality, performance, jejunum morphology, ileal microbial population, and apparent metabolizable energy in broilers. According to the completely randomized design (CRD) in a factorial arrangement 2*3 (conditioning temperatures: 65 and 75 °C; conditioning times: 30, 60, and 90 second), 540 one-day-old male Ross 308 broilers were randomly distributed among six treatments with six replications, each replicate including 15 birds. Treatments included: 1) 65-30, 2) 65-60, 3) 65-90, 4) 75-30, 5) 75-60, 6) 75-90. The results showed that 60 seconds of conditioning at 75 °C increased the pellet durability index (PDI) in the starter diets (p<0.05). In the grower and finisher diets, groups (65-60) and (65-90) showed the highest PDI (p<0.05). Broilers fed diets conditioned at 75 °C for 60 s showed more body weight gain (p<0.05). On days 25 and 42, the highest villus height (VH) was observed in treatment (75-60), and 60 s steam conditioning increased crypt depth (CD) (p<0.05). At 75 oC, the number of goblet cells decreased, while their highest number was observed at 30 and 60 s on 25 d (p<0.05). Conditioning at 75 °C for 60 s enhanced the apparent metabolizable energy (AME) in broilers (p<0.05). In conclusion, 60 s conditioning at 75 °C improved the PDI of starter diets, performance, villus height, and AME, while the suitable temperature and pelleting time for grower and finisher diets were (65-60) and (65-90).(AU)


Assuntos
Animais , Temperatura , Galinhas/fisiologia , Microbioma Gastrointestinal/fisiologia , Condicionamento Físico Animal
11.
Rev. bras. zootec ; 52: e20220079, 2023. tab
Artigo em Inglês | VETINDEX | ID: biblio-1436798

Resumo

The objective of this study was to evaluate the effects of different levels of microencapsulated carvacrol and cinnamaldehyde on metabolizable energy, feed digestibility, intestinal morphometric analysis, and antioxidant parameters in broilers. A completely randomized design with five treatments and eight replications of eight broilers (Cobb male) was used, and collections were carried out at 20 and 42 days of age. Carvacrol and cinnamaldehyde concentrations (mg/kg) were: 0 (control), 50, 100, 150, and 200. Carvacrol and cinnamaldehyde improved apparent metabolizable energy but did not change protein and ether extract digestibility. Supplementation increased intestinal villus height and villi:crypt ratio; in which 100 mg/kg produced the greatest villus height. Serum uric acid levels were lower in birds receiving supplementation. Improvement in the activity of glutathione peroxidase and glutathione-S-transferase was observed, while lower uric acid, thiobarbituric acid reactive substances, and reactive oxygen species levels were observed. Microencapsulated carvacrol and cinnamaldehyde improve apparent metabolizable energy and can be administered in broiler feed without risk to the bird's health. These supplements may serve as alternative products to aid the performance of commercial poultry.


Assuntos
Animais , Galinhas , Suplementos Nutricionais , Dieta/veterinária , Ração Animal , Antioxidantes
12.
Rev. bras. ciênc. avic ; 25(2): eRBCA-2022-1722, 2023. tab
Artigo em Inglês | VETINDEX | ID: biblio-1427942

Resumo

The knowledge of biological trace minerals and phytase requirements for modern broiler genotypes is not established and the pressure to reduce their usage in animal feeding due to environmental issues is increasing. Here, the alkaline phosphatase (ALP) and tartarate-resistant acid phosphatase (TRAP) of the tibia and serum of broilers fed with diets containing various levels of phytase and reduced levels of zinc, manganese, and copper was evaluated. The experiment was performed using 1,200 male Cobb broilers raised according to standard commercial husbandry techniques. Data were analyzed as a 4×3 factorial arrangement with four concentrations of zinc (0.34, 0.49, 0.64, and 0.79 ppm), manganese (0.18, 0.43, 0.68, and 0.93 ppm), or copper (0.09, 0.12, 0.15, and 0.18 ppm) and three concentrations of phytase (0, 500, and 1,000 FTU/kg) for age periods of 1-21 and 36-42 days. While the dietary supplementation with copper did not induce a significant effect in bone tissue biochemical markers, serum TRAP activity of 42-day old broilers increased with higher copper levels. Increasing dietary zinc levels linearly increased ALP activity in tibia growth, suggesting that zinc is essential for longitudinal bone growth. Phytase significantly promoted the increase of TRAP and ALP activities, suggesting that manganese increased growth plate activity, accelerated calcification, and remodeled the newly formed tissue into trabecular bone. Although not every enzymatic activity was affected by the treatments, the phytase use, along with trace minerals, improved the animal response to the rapid growth required nowadays and provided the nutrients for adequate bone metabolism.(AU)


Assuntos
Animais , Galinhas/fisiologia , Monoéster Fosfórico Hidrolases/síntese química , Zinco/efeitos adversos , Cobre/efeitos adversos , Manganês/efeitos adversos
13.
Rev. bras. ciênc. avic ; 25(2): eRBCA-2022-1743, 2023. tab
Artigo em Inglês | VETINDEX | ID: biblio-1419079

Resumo

The purpose of this study was to investigate the effect of sunflower kernel peptides produced by enzymatic digestion, fermentation, or both on the growth performance, nutrient digestibility, and health status of broilers. Four diets contained 20% of sunflower kernel meal as its raw form (CON) or degraded by protease (ESM), Bacillus pumilus (FSM), or both (DSM). A total of 480 yellow broilers at one day old were randomly distributed to 4 groups with 6 replicates of 20 chicks each. The feeding trial lasted for 63 d. Results showed that peptide content was increased (p<0.001) from 3.97% (CON) to 32.5% (ESM), 24.2% (FSM), and 39.1% (DSM). The three sunflower peptide groups improved (p≤0.001) feed intake and body weight gain. The peptide groups increased (p≤0.015) ileal apparent digestibility of dry matter, energy, crude protein, and amino acids (methionine, lysine, tryptophan, and threonine). Furthermore, the peptide groups improved (p≤0.029) the health status by increasing serum immunoglobulins (IgA, IgG) and glutathione peroxidase. Additionally, among the peptide groups, DSM showed more pronounced effects (p<0.05) on these parameters than ESM or FSM. It is concluded that dual-degradation by enzymolysis and fermentation has a better improvement in the nutrition and application of sunflower kernel meal in broilers.(AU)


Assuntos
Animais , Peptídeos/efeitos adversos , Galinhas/fisiologia , Fenômenos Fisiológicos da Nutrição Animal , Helianthus/química , Valor Nutritivo
14.
Rev. bras. ciênc. avic ; 25(2): eRBCA-2022-1721, 2023. tab, graf
Artigo em Inglês | VETINDEX | ID: biblio-1427958

Resumo

In this study, we investigated the effects of Ganoderma lucidum extract (GLE) supplementation on the cecal microbiota of broilers challenged with dextran sulfate sodium (DSS) during the starter phase. A total of 32 one-day-old, unsexed broiler chicks were randomly divided into four dietary treatments with eight birds per treatment and reared individually for 14 days (n = 8). The diet treatments were: non-DSS challenge, DSS challenge only, DSS challenge plus 0.5 mL/L GLE, and DSS challenge plus 1 mL/L GLE. The results showed that DSS challenge plus 0.5 mL GLE alleviated inflammatory gene expression in the duodenum of broilers (p≤0.01). The alpha diversity of bacterial species in the cecal digesta increased in the group treated with DSS plus 1 mL/L GLE compared with the DSS challenge-only group (p≤0.01). Principal component analysis and principal coordinate analysis indicated distinct clusters between groups treated with DSS-only and DSS plus GLE (0.5 and 1 mL/L). The abundance of the genera Ruminiclostridium 9, Enterococcus, and Sellimonas increased in the group treated with DSS plus GLE (0.5 and 1 mL/L) compared with the other groups (p≤0.01). Comparative microbial function analysis demonstrated that the immune system was promoted in the group treated with DSS plus GLE (0.5 and 1 mL/L) compared to the DSS challenge-only group (p≤0.001). These results demonstrated that GLE supplementation can modulate the cecal microbial community of broilers under DSS challenge during the starter phase.(AU)


Assuntos
Animais , Galinhas/microbiologia , Reishi/química , Dextranos/efeitos adversos , Microbiota/fisiologia
15.
Braz. j. biol ; 832023.
Artigo em Inglês | LILACS-Express | LILACS, VETINDEX | ID: biblio-1469070

Resumo

Abstract The poultry sector in Pakistan is contributing mainly in bridging gap between demand and supply for protein. Mycoplasma gallisepticum is an emerging bacterium causing serious problems in poultry industry of Pakistan. A cross-sectional study was conducted to evaluate the M. gallisepticum load in poultry populated regions of Pakistan. Total 600 serum and 600 swab samples were collected, 200 from each broiler, layers and breeders poultry in Rawalpindi and Abbottabad districts. Serum samples were analyzed through ELISA for seroprevalence. Swabs were cultured on Freys medium followed by PCR and partial mgc2 gene sequencing. Results of seroprevalence of M. gallisepticum showed that layers (75%, n=150) are more positive as compared to breeders (70%, n=140) and broilers (50%, n=100). Typical colonies of the M. gallisepticum were observed in breeder (26.5%), followed by layer (21%) and broilers (9%). A total of 37.1% (n=42) samples were identified positive through PCR out of total 113 cultured based positive samples. A total of six M. gallisepticum isolates of current study showed 98-99 percent similarity with previously reported isolates on the basis of mgc2 gene partial sequencing. The M. gallisepticum was found highly prevalent in different poultry breads. Results of this study would add into basic data and provide a direction for livestock sector to strengthen a control strategy for mycoplasmosis in poultry farms.


Resumo O setor avícola do Paquistão está contribuindo principalmente para preencher a lacuna entre a demanda e a oferta de proteína. Mycoplasma gallisepticum é uma bactéria emergente que causa sérios problemas na indústria avícola do Paquistão. Um estudo transversal foi conduzido para avaliar a carga de M. gallisepticum em regiões de avicultura do Paquistão. Um total de 600 amostras de soro e 600 amostras de esfregaço foi coletado, 200 de cada frango de corte, poedeiras e aves reprodutoras nos distritos de Rawalpindi e Abbottabad. Amostras de soro foram analisadas por ELISA para soroprevalência. As zaragatoas foram cultivadas em meio Frey, seguido de PCR e sequenciação parcial do gene mgc2. Os resultados da soroprevalência de M. gallisepticum mostraram que as poedeiras (75%, n = 150) são mais positivas em comparação com matrizes (70%, n = 140) e frangos de corte (50%, n = 100). Colônias típicas de M. gallisepticum foram observadas em reprodutoras (26,5%), seguidas de poedeiras (21%) e frangos de corte (9%). Um total de 37,1% (n = 42) das amostras foi identificado como positivas por PCR de um total de 113 amostras positivas baseadas em cultura. Um total de seis isolados de M. gallisepticum do estudo atual mostrou 98-99% de similaridade com isolados relatados anteriormente com base no sequenciamento parcial do gene mgc2. O M. gallisepticum foi encontrado com alta prevalência em diferentes pães de aves. Os resultados deste estudo acrescentariam dados básicos e forneceriam orientação para o setor pecuário fortalecer uma estratégia de controle da micoplasmose em granjas avícolas.

16.
Arq. bras. med. vet. zootec. (Online) ; 75(2): 280-292, Mar.-Apr. 2023. tab
Artigo em Inglês | VETINDEX | ID: biblio-1434119

Resumo

The study was carried out with the objective of evaluating the effects of using phytase levels at different energy densities in the diet of broilers, from 28 to 35 days of age. The experimental diets contained increasing levels of apparent metabolizable energy corrected for nitrogen balance and different levels of phytase. Growth performance variables, nutrient digestibility, as well as variables related to bone integrity were examined. Diets containing 500 FTU/kg in combination with 3150kcal.kg-1 of AMEn resulted in better growth performance. Supplementation with 500 and 1000 FTU/kg in the diets provide better coefficients of apparent and ileal digestibility of calcium and phosphorus, but negatively influence the deposition of calcium, phosphorus, dry matter, and ash in the tibia, in addition to adversely affecting the breakage and area of these bones in broilers. Bone length and the levels of magnesium and zinc present in the tibias were not influenced by the energy and phytase levels of the diets. There is no interaction between the different energetic densities and the phytase doses in the variables analyzed in the studied period. Increasing the energy density of diets resulted in improved apparent and ileal digestibility for most nutrients.


O estudo foi realizado com o objetivo de avaliar os efeitos da utilização de níveis de fitase em diferentes densidades energéticas na dieta de frangos de corte, no período de 28 a 35 dias de idade. As dietas experimentais contiveram níveis crescentes de energia metabolizável aparente corrigida para balanço de nitrogênio e diferentes níveis de fitase. Foram avaliadas variáveis de desempenho zootécnico, digestibilidade de nutrientes e variáveis relacionadas à integridade óssea. Dietas contendo 500 FTU/kg de fitase em combinação com 3150kcal.kg-1 de EMAn resultam em melhor desempenho zootécnico. A suplementação com 500 e 1000 FTU/kg nas dietas propicia melhores coeficientes de digestibilidade aparente e ileal do cálcio e do fósforo, mas influencia negativamente na deposição de cálcio, fósforo, matéria seca e cinzas das tíbias, além de afetar adversamente a quebra e a área desses ossos em frangos de corte. O comprimento do osso e os níveis de magnésio e zinco presentes nas tíbias não são influenciados pelos níveis energéticos e de fitase das dietas. Não há interação entre as diferentes densidades energéticas e as doses de fitase nas variáveis analisadas no período estudado. O aumento da densidade energética das dietas resulta em melhora na digestibilidade aparente e ileal para a maioria dos nutrientes.


Assuntos
Animais , Galinhas , Dieta/veterinária , Aditivos Alimentares
17.
Braz. j. biol ; 83: e250296, 2023. tab, graf
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1339385

Resumo

Abstract The current study aimed to determine the effects of different levels of Zingiber officinale as a herbal feed additive on growth performance, carcass characteristic, serum biochemistry, total bacterial count (TBC), gut morphology, and immunological parameters of broilers. A total of 1500, day-old broiler chicks (Hubbard) were equally accredited to five treatment groups, each with six replicates (50 birds/replicate). Five experimental diets were prepared using basal diet i.e. with antibiotics positive control (PC), 3 g/kg ginger (group A), 6 g/kg ginger (group B), 9 g/kg ginger (group C) and without antibiotics negative control (NC). Group A and C showed significantly (p<0.05) higher feed intake (FI) as compared to other groups. Group C showed significantly (p<0.05) lower Total bacterial count (TBC) followed by group B as compared to NC. Carcass characteristics showed non-significant effects among different treatments. Mean villi length and width were significantly (p <0.05) higher in all ginger supplemented groups as compared to the control groups. Blood serum parameters including cholesterol, triglycerides, and low-density lipoproteins (LDL) were significantly (p<0.05) lower in groups B and C in comparison with the control groups. Whereas high-density lipoproteins (HDL) was significantly higher in group B as compared to the others. In conclusion, ginger supplementation @0.6% in the basal diet significantly improved growth performance and gut morphometry of broilers. It also showed a positive impact on cholesterol, triglycerides and gut microbes. Therefore, ginger could be a better substitute for antibiotic growth promoters.


Resumo O presente estudo teve como objetivo determinar os efeitos de diferentes níveis de Zingiber officinale como aditivo à base de plantas medicinais sobre o desempenho de crescimento, características da carcaça, bioquímica sérica, contagem bacteriana total (CBT), morfologia intestinal e parâmetros imunológicos de frangos de corte. Um total de 1.500 pintos de corte de um dia de idade (Hubbard) foram igualmente credenciados em cinco grupos de tratamento, cada um com seis repetições (50 aves/repetição). Cinco dietas experimentais foram preparadas usando dieta basal, ou seja, com controle positivo de antibióticos (PC), 3 g/kg de gengibre (grupo A), 6 g/kg de gengibre (grupo B), 9 g/kg de gengibre (grupo C) e sem controle negativo de antibióticos (NC). Os grupos A e C apresentaram consumo de ração (FI) significativamente (p < 0,05) maior do que os outros grupos. O grupo C apresentou contagem bacteriana total (CBT) significativamente menor (p < 0,05) seguido pelo grupo B em comparação com o NC. As características da carcaça apresentaram efeitos não significativos entre os diferentes tratamentos. O comprimento e largura médios das vilosidades foram significativamente (p < 0,05) maiores em todos os grupos suplementados com gengibre em comparação com os grupos de controle. Os parâmetros séricos do sangue, incluindo colesterol, triglicerídeos e lipoproteínas de baixa densidade (LDL), foram significativamente (p < 0,05) menores nos grupos B e C em comparação com os grupos controle. Enquanto as lipoproteínas de alta densidade (HDL) foram significativamente maiores no grupo B em comparação com os outros. Em conclusão, a suplementação de gengibre a 0,6% na dieta basal melhorou significativamente o desempenho de crescimento e a morfometria intestinal de frangos de corte. Ele também mostrou um impacto positivo sobre o colesterol, triglicerídeos e micróbios intestinais. Portanto, o gengibre pode ser um substituto melhor para os promotores de crescimento com antibióticos.


Assuntos
Animais , Zingiber officinale , Galinhas , Suplementos Nutricionais , Dieta/veterinária , Ração Animal/análise
18.
Acta sci., Anim. sci ; 45: e58287, 2023. tab, graf
Artigo em Inglês | VETINDEX | ID: biblio-1413097

Resumo

The study aimed to evaluate performance and growth curves of broilers fed different nutritional relations. A total of 1,440 Cobb-500 male day-old chicks were assigned to eight treatments in a 2 x 2 x 2 factorial arrangement with six replicates of 30 birds each. The main factors were nutritional density (control and high), lysine source (HCl and sulfate), and calcium pidolate (presence and absence). Analyses were made for body weight gain (BWG), and feed conversion rate (FCR) at 21, and 42 days of age. The growth curves were adjusted by weighing a bird per plot every three days. Data for BWG were tested by ANOVA to evaluatethe effects of treatments and their interactions at 5% significance, and the Gompertz model was adjusted by NLS. Birds fed a high nutritional density had higher BWG and lower FCR. Calcium pidolate and different sources of lysine did not influence the FCR of broilers, however a triple interaction was evidenced for BWG at 1 to 42 days of age. The day with maximum gain adjusted by Gompertz of all treatments was at the 32ndday of age and the maximum weight (A) was around 5.85 kg.(AU)


Assuntos
Animais , Galinhas/fisiologia , Ingestão de Alimentos/fisiologia , Dinâmica não Linear , Valor Nutritivo
19.
Acta sci. vet. (Impr.) ; 51(supl.1): Pub. 852, 2023. ilus
Artigo em Inglês | VETINDEX | ID: biblio-1418139

Resumo

Background: A cutaneous or superficial myxoma is a benign neoplasm of dermal or subcutaneous fibroblast origin. Although rare, it has been previously described in several species, including poultry. It presents as a single node or soft mass with a gelatinous cut surface. Histopathological analysis is essential for diagnosis and to differentiate it from other mesenchymal neoplasms and inflammatory or degenerative processes. Microscopically, it consists of dermal or subcutaneous lobules of plump, stellate, or spindle-shaped, bland-looking cells embedded in a basophilic myxoid matrix. This report describes the pathological findings in a rare case of cutaneous myxoma in a 42-day-old broiler flock. Cases: During ante mortem inspection of a 42-day-old broiler flock at a slaughterhouse under the authority of the Federal Inspection Service (southern Brazil), nodular lesions or encrusted areas with yellow and black areas were observed in the head skin of less than 1% of animals. These lesions, approximately 0.5 cm in diameter, were observed on the comb, in the periocular skin region, and close to the animals' nostrils. During the breeding period, no health or epidemiological events were observed. Fragments of the lesions in the comb and periocular skin were collected and fixed in buffered 10% formalin. The samples were sent to the laboratory, routinely processed, and stained with hematoxylin and eosin and Alcian blue. Microscopically, the lesions consisted of irregular multifocal proliferation of connective tissue showing spindle cells with poorly demarcated borders and scarce cytoplasm in a slightly basophilic myxoid aspect matrix. The adjacent epidermis is compressed due to neoplastic proliferation. No areas of epithelial hyperplasia or inclusion bodies were observed. According to the pathologic description and considering its descriptive epidemiology, our main clinical suspicion was cutaneous fowl pox, a pathology characterized by the appearance of nodules in regions devoid of feathers. However, the microscopic changes observed were compatible with those described for cutaneous myxomas. In addition, the extracellular matrix was positive for Alcian Blue staining, which is an indicator of myxoma. In the present case, the SIF did not report the same macroscopic lesions in other flocks of the same origin. Discussion: Connective tissue tumors, including myxomas, occur considerably less frequently under field conditions. In addition, these neoplasms are more frequent in mature birds and are not usually described in broilers, as observed in this report. The cutaneous myxoma described in broilers is usually a sporadic neoplasm that does not cause zootechnical losses, as observed in the case report. Its etiology is unknown and has been associated with various factors, such as local trauma and foreign bodies. Some fragments of plant material from the breeding environment were microscopically detected in the encrusted areas, which may indicate previous trauma or a foreign body. Myxoma has been associated with avian leukosis virus (ALV) subgroup A, but SIF did not report the same macroscopic lesions in other flocks of the same breeder hen's origin in the present case. Furthermore, sporadic connective tissue tumors associated with the virus occur in mature chickens but not in broilers. Myxoma lesions should be considered in the differential diagnosis of other connective tissue tumors and infectious agents that cause lesions in the skin and subcutaneous tissue.


Assuntos
Animais , Galinhas/lesões , Mixoma/veterinária , Abate de Animais , Neoplasias de Tecido Conjuntivo/veterinária
20.
Rev. bras. ciênc. avic ; 25(2): eRBCA-2022-1668, 2023. tab
Artigo em Inglês | VETINDEX | ID: biblio-1419081

Resumo

This study investigated the effect of resveratrol on the immune and inflammatory responses and the mRNA levels of splenic toll-like receptor (TLR)-4 signaling pathway-related genes of broilers under heat stress (HS). One hundred and sixty-two birds were allocated to three groups, each with 6 replicates, for 21 continuous days. The three treatments were as follows: the control group (22 ± 1 °C), the HS (33 ± 1 °C for 10 h d-1 and 22 ± 1 °C for the remaining time) group and the HS + resveratrol (400 mg kg-1) group. At the end of the trial, one bird per replicate close to the average body weight (BW) was selected, exsanguinated, and slaughtered. Compared with the control group, the HS treatment decreased (p<0.05) final BW, average daily gain (ADG), average daily feed intake (ADFI), relative weight of bursa of Fabricius and spleen, serum immunoglobulin (Ig) Y, IgA and interleukin (IL)-10 contents, and splenic IL-10 mRNA level, while it increased (p<0.05) feed/gain, mRNA levels of splenic tumor necrosis factor-α (TNF-α), TLR-4, nuclear factor-kappa-B (NF-κB), IL-1ß, and IL-6. Compared to the HS group, the HS+resveratrol group exhibited increased (p<0.05) final BW, ADG, relative weight of bursa of Fabricius and spleen, serum IgY, IgA and IL-10 contents, and splenic IL-10 mRNA level, while it exhibited lower (p<0.05) TNF-α, IL-1ß and IL-6 contents in serum, and splenic TLR4, TNF-α, IL-1ß, and NF-κB mRNA levels. In conclusion, resveratrol prevented a HS-impairment of the immune function of broilers by blocking the abnormal activation of the TLR4 signaling pathway.(AU)


Assuntos
Animais , Galinhas/imunologia , Resposta ao Choque Térmico/imunologia , Resveratrol/efeitos adversos , Receptor 4 Toll-Like/análise
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