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1.
Acta cir. bras ; 37(2): e370207, 2022. tab, graf, ilus
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1374069

Resumo

Purpose: To analyze the effect of high-intensity interval training (HIIT) on bone mineral density (BMD) in a model of type 2 diabetes mellitus. Methods: Thirty-two male, adult, 12-week-old rats (Rattus norvegicus), of the Wistar lineage, were used. The animals induced to the experimental model received a high fat diet for 10 days and, after that period, intraperitoneal injection of streptozotocin (40 mg·kg­1), dissolved in 20 mmol·L­1 sodium citrate solution (pH = 4.5). The experimental group of diabetes was formed by the animals that, 48 h after the injection of streptozotocin, had fasting blood glucose > 250 mg·dL­1). The animals were randomly divided into four groups with eight animals each: HIIT experimental diabetes; HIIT control; sedentary experimental diabetes and sedentary control. The animals in the HIIT group performed an aerobic exercise protocol on a treadmill inclined at an angle of 15° to the horizontal, with interspersed intensity. Five weekly sessions, lasting 49 min each, were held for 6 weeks. The analysis of cortical bone density (CBD) and BMD were performed by X-ray images using the In-Vivo Xtreme II/Bruker system. Results: For CBD and BMD, when comparing diabetes and control groups, a significant difference was seen between groups in relation to HIIT (p = 0.007). Animals submitted and not submitted to HIIT in the same group showed a significant difference between groups in relation to diabetes (p < 0.001). Conclusions: The HIIT experimental diabetes group had increased CBD and BMD in comparison with the sedentary experimental diabetes group.


Assuntos
Animais , Masculino , Ratos , Osteoporose/etiologia , Densidade Óssea , Diabetes Mellitus Experimental/complicações , Diabetes Mellitus Tipo 2/complicações , Treinamento Intervalado de Alta Intensidade/veterinária , Ratos Wistar
2.
Acta amaz ; 52(1): 53-59, 2022. ilus, graf, tab
Artigo em Inglês | VETINDEX | ID: biblio-1437374

Resumo

Copaiba oleoresin, extracted from the Copaifera reticulata tree, has been used as a remedy in popular medicine in the Brazilian Amazon for various purposes, including reducing drug abuse. Yet no studies evaluated the effect of repeated administration of copaiba oil on alcohol consumption in animals. To evaluate this effect, we divided adult male Wistar rats into a) an alcohol group in which the animals had free access to choose between two bottles: one containing alcohol solution (20%) and another containing vehicle solution (0.2% saccharin); and b) a control group with access to two bottles containing vehicle solution. Rats were free to drink 24 h per day, for 35 days. Daily alcohol consumption and weekly body weight gain and food intake were monitored. From day 22, half of the rats in each group received 600 mg kg-1 copaiba oleoresin and the other received vehicle, subcutaneously, once a day, for three days. On day 35, rats were evaluated in an open-field test. The results showed that copaiba oil decreased voluntary alcohol intake and preference between days 2 and 6 after the last administration. Copaiba treatment also decreased the food intake and body weight gain in both alcohol and control groups without changing behaviors in the open-field test. Therefore, copaiba oil was able to reduce voluntary alcohol consumption in rats and could be tested in humans as an adjuvant to treat alcohol use disorder.(AU)


O óleo extraído da árvore copaíba, Copaifera reticulata, tem sido usado na medicina popular na Amazônia brasileira para diversos fins, incluindo abuso de drogas. Contudo, não há estudos avaliando o efeito da administração repetida do óleo de copaíba sobre o consumo de álcool em animais. Para avaliar esse efeito, dividimos ratos Wistar machos adultos em dois grupos: a) um grupo álcool, no qual os animais tinham livre acesso a duas garrafas: uma contendo solução alcoólica (20%) e outra contendo solução veículo (sacarina 0,2%); e b) um grupo controle com acesso a duas garrafas contendo solução veículo. Os ratos podiam beber livremente, 24 horas por dia, durante 35 dias. O consumo diário de álcool, bem como o ganho de peso corporal semanal e a ingestão de alimentos foram monitorados. A partir do dia 22, metade dos ratos de cada grupo recebeu 600 mg kg-1 de óleo de copaíba e a outra metade recebeu veículo, por via subcutânea, uma vez ao dia, durante três dias. No dia 35, os ratos foram testados em teste de campo aberto. Os resultados mostraram que o óleo de copaíba diminuiu a ingestão voluntária e a preferência por álcool entre os dias 2 e 6 após a última administração. O tratamento com óleo de copaíba também diminuiu a ingestão alimentar e o ganho de peso corporal em ambos os grupos álcool e controle, sem alterar o comportamento no teste de campo aberto. Portanto, o óleo de copaíba foi capaz de reduzir o consumo voluntário de álcool em ratos e poderia ser testado em humanos como um adjuvante para tratar transtorno de uso de álcool.(AU)


Assuntos
Animais , Ratos , Consumo de Bebidas Alcoólicas/efeitos adversos , Óleos Voláteis , Produtos Biológicos , Fabaceae/química
3.
Acta cir. bras ; 37(5): e370503, 2022. tab, ilus
Artigo em Inglês | VETINDEX | ID: biblio-1383307

Resumo

Purpose: To investigate the effect of givinostat treatment in acetic acid-induced ulcerative colitis model in rats. Methods: Thirty male Wistar albino rats were used. Rats were randomly divided into three equal groups, and colitis was induced on 20 rats by rectal administration of %4 solutions of acetic acid. Twenty rats with colitis were randomly divided into two groups. %0.9 NaCl (saline) solution was administered intraperitoneally to the first group of rats (saline group, n=10) at the dose of 1 mL/kg/day. Givinostat was administered intraperitoneally to the second group rats (Givinostat group, n=10) at the dose of 5 mg/kg/day. Samples were collected for biochemical analysis. Colon was removed for histopathological and biochemical examinations. Results: Plasma tumor necrosis factor-α (TNF-α), pentraxin-3 (PTX-3), and malondialdehyde levels were significantly decreased in the givinostat group compared to the saline group (p<0.05, p<0.001, and p<0.001 respectively; p<0.001, p<0.001, and p<0.001, respectively). Colon TNF-α and prostaglandin F2 alpha (PGF-2) levels were significantly decreased (p<0.05, and p<0.001, respectively). The givinostat group had a significantly lower histologic score than saline group (p<0.001, and p<0.001, respectively). Conclusions: Givinostat, a good protector and regenerator of tissue and an anti-inflammatory agent, may be involved in the treatment of colitis in the future.


Assuntos
Animais , Ratos , Regeneração , Terapêutica , Colite Ulcerativa , Anti-Inflamatórios
4.
Acta cir. bras ; 37(10): e371004, 2022. graf, tab
Artigo em Inglês | VETINDEX | ID: biblio-1415430

Resumo

Purpose: The present study explored the role and mechanism involved in aprepitant-induced cardioprotective effects in rat model of ischemia-reperfusion injury. Methods: The isolated hearts of Wistar male albino rats were subjected to ischemia-reperfusion injury on Langendorff apparatus. The extent of myocardial injury was assessed by measuring lactate dehydrogenase 1 and CK-MB release in the coronary effluent. The rats were treated with aprepitant (5, 10 and 20 mg/kg) before isolating hearts. After injury, the levels of HIF-1α, p-AkT, p-GSK-3ß/GSK-3ß were measured in heart homogenates. LY294002 was employed as PI3K inhibitor. Results: Ischemia-reperfusion led to significant myocardial injury and decreased the levels of HIF-1α, p-AkT and ratio of p-GSK-3ß/GSK-3ß. Aprepitant attenuated myocardial injury and restored the biochemical changes in a dose-dependent manner. Pre-treatment with LY294002 (10 and 20 mg/kg) abolished aprepitant-mediated cardioprotective effects and restored the biochemical parameters in the heart homogenate. Conclusions: Aprepitant may be effective in preventing ischemia-reperfusion-induced myocardial injury, which may be due to activation of PI3K-AkT-GSK-3ß and HIF-1α signaling pathway.


Assuntos
Animais , Ratos , Cardiotônicos , Traumatismo por Reperfusão , Aprepitanto/administração & dosagem , Isquemia
5.
Acta cir. bras ; 37(9): e370901, 2022. tab, graf, ilus
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1402981

Resumo

Purpose: To evaluate the effect of preoperative intravenous chemotherapy with 5-fluorouracil on liver regeneration in an experimental model of major hepatectomy in rats. Methods: Wistar rats were divided into two groups of 20 animals each and submitted to 70% hepatectomy 24 h after intravenous injection of 5-fluorouracil 20 mg/kg (fluorouracil group, FG) or 0.9% saline (control group, CG). After hepatectomy, each group was subdivided into two subgroups of 10 animals each according to the day of sacrifice (24 h or 7 days). Liver weight during regeneration, liver regeneration rate using Kwon formula, and the immunohistochemical markers proliferating cell nuclear antigen (PCNA) and Ki-67 were used to assess liver regeneration. Results: At early phase (24 h after hepatectomy) it was demonstrated the negative effect of 5-fluorouracil on liver regeneration when assessed by Kwon formula (p < 0.0001), PCNA analysis (p = 0.02). With regeneration process complete (7 days), it was possible to demonstrate the sustained impairment of chemotherapy with 5-fluorouracil on hepatocytes regeneration phenomenon when measured by Kwon formula (p = 0.009), PCNA analysis (p = 0.0001) and Ki-67 analysis (0.001). Conclusions: Preoperative chemotherapy with intravenous 5-fluorouracil negatively affected the mechanisms of liver regeneration after major hepatectomy in rats.


Assuntos
Animais , Ratos , Quimioprevenção/métodos , Fluoruracila/uso terapêutico , Hepatectomia/reabilitação , Regeneração Hepática/efeitos dos fármacos
6.
Acta cir. bras ; 36(9): e360908, 2021. tab
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1345028

Resumo

ABSTRACT Purpose: To describe a new anesthetic protocol medullary and nerve roots access and in Rattus norvegicus. Methods: Seventy female Wistar rats (n=70) were used. The animals were randomly divided into two laminectomy groups: cervical (n=40) and thoracic (n=30). In cervical group, a right posterior hemilaminectomy was performed to access the nerve roots. In thoracic group, a laminectomy of the eighth thoracic vertebra was accomplished. Thirty-five rats (20 cervical and 15 thoracic) were submitted to old anesthetic protocol (ketamine 70 mg/kg plus xylazine 10 mg/kg); and the 35 other animals (20 cervical and 15 thoracic) were submitted to a new anesthetic protocol (ketamine 60 mg/kg,xylazine 8 mg/kg and fentanyl 0.03 mg/kg). Results: The time to complete induction was 4.15 ±1.20 minin ketamine, xylazine and fentanyl group, and it was 4.09 ±1.47 min in the ketamine and xylazine group. There was no correlation in the time required to perform the cervical laminectomy in the old anesthetic protocol. In all groups, the animals submitted to the old anesthetic protocol had a higher level of pain on the first and third postoperative days than the animals submitted to the new anesthetic protocol. Conclusions: The new anesthetic protocol reduces the surgical time, allows better maintenance of the anesthetic plan, and brings more satisfactory postoperative recovery.


Assuntos
Animais , Ratos , Ketamina , Anestésicos , Xilazina , Ratos Wistar
7.
Acta cir. bras ; 36(3): e360304, 2021. tab, graf
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1284908

Resumo

ABSTRACT Purpose To evaluate the effect of fractional carbon dioxide (CO2) laser treatment using lower power associated with pulse stacking within collagen fibers, using second harmonic generation microscopy and computerized image analysis. Methods Twenty male Wistar rats aging eight weeks were used. Each treatment area received a single-pass CO2 fractional laser with different parameters. The 20 animals were divided into two groups and euthanized after 30 and 60 days. Second harmonic generation images were obtained and program ImageJ was utilized to evaluate the collagen organization within all areas. Collagen anisotropy, entropy and optical density were quantified. Results Increased anisotropy over time was observed in all four areas, but only reached statistical significance (p = 0.0305) when the mildest parameters were used (area four). Entropy decreased over time in all areas, but without significance(p = 0.1779) in area four. Density showed an overtime increase only in area four, but no statistical significance was reached (p = 0.6534). Conclusions When combined, the results obtained in this study regarding anisotropy, entropy and density tend to demonstrate that it is possible to achieve collagen remodeling with the use of lower power levels associated with stacked pulses.


Assuntos
Animais , Masculino , Ratos , Envelhecimento da Pele , Lasers de Gás , Microscopia de Geração do Segundo Harmônico , Pele , Dióxido de Carbono , Ratos Wistar
8.
Acta cir. bras ; 36(6): e360605, 2021. tab, graf
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1284912

Resumo

ABSTRACT Purpose To evaluate and compare two types of different scaffolds in critical bone defects in rats. Methods Seventy male Wistar rats (280 ± 20 grams) divided into three groups: control group (CG), untreated animals; biomaterial group 1 (BG1), animals that received the scaffold implanted hydroxyapatite (HA)/poly(lactic-co-glycolic) acid (PLGA); and biomaterial group 2 (BG2), animals that received the scaffolds HA/PLGA/Bleed. The critical bone defect was induced in the medial region of the skull calotte with the aid of an 8-mm-diameter trephine drill. The biomaterial was implanted in the form of 1.5 mm thick scaffolds, and samples were collected after 15, 30 and 60 days. Non-parametric Mann-Whitney test was used, with the significance level of 5% (p ≤ 0.05). Results Histology revealed morphological and structural differences of the neoformed tissue between the experimental groups. Collagen-1 (Col-1) findings are consistent with the histological ones, in which BG2 presented the highest amount of fibers in its tissue matrix in all evaluated periods. In contrast, the results of receptor activator of nuclear factor kappa-Β ligand (Rank-L) immunoexpression were higher in BG2 in the periods of 30 and 60 days, indicating an increase of the degradation of the biomaterial and the remodeling activity of the bone. Conclusions The properties of the HA/PLGA/Bleed scaffold were superior when compared to the scaffold composed only by HA/PLGA.


Assuntos
Animais , Masculino , Ratos , Materiais Biocompatíveis , Alicerces Teciduais , Osteogênese , Regeneração Óssea , Ratos Wistar , Copolímero de Ácido Poliláctico e Ácido Poliglicólico
9.
J. Anim. Behav. Biometeorol. ; 09(01): 1-5, Jan. 2021. graf
Artigo em Inglês | VETINDEX | ID: vti-765632

Resumo

In the present work, we sought to determine the effects of an individual application of prenatal stress and postnatal stress and their combination on the anxious and locomotor behavior of first-time mother rats. Indeed, three batches of rats were exposed to three types of daily repeated stress: one batch exposed to prenatal restraint stress (SC): 01h a day from day 11 to day 19 of gestation, one batch exposed to postnatal mother prenatal stress (SS): 03h a day from the third to the fourteenth postnatal day (JPN), and one batch exposed to combined restraint stress: 01h from day 11 to day 19 of gestation followed by a postnatal mother separation of 03h a day from the third to fourteenth JPN (CSS); a fourth control batch (T) of female rats that did not undergo any type of stress was also part of our study. At postnatal days 15 and 20, the behavior of the female rats in the four batches was assessed using the Open field Test along with the Plus Maze, and at JPN 30, the female rats were decapitated, the blood of which was collected for plasma adrenocorticotropic hormone (ACTH) level evaluation. Our findings show that all studied behaviors were very highly affected relative to the results of the control batch after individually applying prenatal restraint stress and mother separation. We noticed an intensified anxious behavior in CSS with a significant increase in plasma levels of ACTH.(AU)


Assuntos
Animais , Masculino , Feminino , Gravidez , Ratos , Comportamento Animal , Transtornos Relacionados a Trauma e Fatores de Estresse/sangue , Prenhez/psicologia , Ratos Wistar
10.
J. Anim. Behav. Biometeorol ; 9(1): 1-5, Jan. 2021. graf
Artigo em Inglês | VETINDEX | ID: biblio-1484337

Resumo

In the present work, we sought to determine the effects of an individual application of prenatal stress and postnatal stress and their combination on the anxious and locomotor behavior of first-time mother rats. Indeed, three batches of rats were exposed to three types of daily repeated stress: one batch exposed to prenatal restraint stress (SC): 01h a day from day 11 to day 19 of gestation, one batch exposed to postnatal mother prenatal stress (SS): 03h a day from the third to the fourteenth postnatal day (JPN), and one batch exposed to combined restraint stress: 01h from day 11 to day 19 of gestation followed by a postnatal mother separation of 03h a day from the third to fourteenth JPN (CSS); a fourth control batch (T) of female rats that did not undergo any type of stress was also part of our study. At postnatal days 15 and 20, the behavior of the female rats in the four batches was assessed using the Open field Test along with the Plus Maze, and at JPN 30, the female rats were decapitated, the blood of which was collected for plasma adrenocorticotropic hormone (ACTH) level evaluation. Our findings show that all studied behaviors were very highly affected relative to the results of the control batch after individually applying prenatal restraint stress and mother separation. We noticed an intensified anxious behavior in CSS with a significant increase in plasma levels of ACTH.


Assuntos
Masculino , Feminino , Animais , Gravidez , Ratos , Comportamento Animal , Prenhez/psicologia , Transtornos Relacionados a Trauma e Fatores de Estresse/sangue , Ratos Wistar
11.
Acta cir. bras. ; 36(3): e360301, 2021. tab, ilus
Artigo em Inglês | VETINDEX | ID: vti-30493

Resumo

Purpose To evaluate the effect of light-emitting diode (LED) in an experimental model of radiodermatitis. Methods Ten male Wistar rats weighing 200250 g were analyzed. Radiation was delivered in a single dose (20 Gy with Strontium-90 dermatological plaques), two areas per animal. After 15 days, they were divided into two groups: control group (n = 5) and LED group (n = 5), which was treated during 21 days later (LED 660 nm, 10 min in alternate days). The endpoints were radiodermatitis scale, histological analysis HE, Picrius Sirius and the gene expression of interleukin-10 (IL-10) and matrix metalloproteinase-9 (MMP-9). Results The LED group showed a higher number of dermal appendages (p = 0.04) and angiogenesis(p = 0.007), a tendency towards higher IL-10 (p = 0.06) and an increase in MMP-9 (p = 0.004) when compared to the control group. Conclusions This study suggested that the use of LED for radiodermatitis increased skin regeneration.(AU)


Assuntos
Animais , Ratos , Lasers Semicondutores , Radiodermite/veterinária , Cicatrização , Regeneração
12.
Acta cir. bras. ; 36(3): e360304, 2021. ilus, tab, graf
Artigo em Inglês | VETINDEX | ID: vti-30576

Resumo

Purpose: To evaluate the effect of fractional carbon dioxide (CO2 ) laser treatment using lower power associated with pulse stacking within collagen fibers, using second harmonic generation microscopy and computerized image analysis. Methods: Twenty male Wistar rats aging eight weeks were used. Each treatment area received a single-pass CO2 fractional laser with different parameters. The 20 animals were divided into two groups and euthanized after 30 and 60 days. Second harmonic generation images were obtained and program ImageJ was utilized to evaluate the collagen organization within all areas. Collagen anisotropy, entropy and optical density were quantified. Results: Increased anisotropy over time was observed in all four areas, but only reached statistical significance (p = 0.0305) when the mildest parameters were used (area four). Entropy decreased over time in all areas, but without significance (p = 0.1779) in area four. Density showed an overtime increase only in area four, but no statistical significance was reached (p = 0.6534). Conclusion: When combined, the results obtained in this study regarding anisotropy, entropy and density tend to demonstrate that it is possible to achieve collagen remodeling with the use of lower power levels associated with stacked pulses.(AU)


Assuntos
Animais , Ratos , Dióxido de Carbono/uso terapêutico , Lasers de Gás/uso terapêutico , Colágeno , Envelhecimento da Pele
13.
Acta cir. bras. ; 36(3): e360302, 2021. ilus, tab
Artigo em Inglês | VETINDEX | ID: vti-30531

Resumo

Purpose To investigate the efficacy of cordycepin, an adenosine analogue, on prevention of esophageal damage and stricture formation due to esophageal caustic burns in rat model comparing with prednisolone. Methods Caustic esophageal burn was introduced by 37.5% of NaOH to distal esophagus. Thirty-two Wistar albino rats were divided in four groups: sham rats undergone laparotomy, treated with 0.9% NaCl; control rats injured with NaOH without cordycepin treatment; cordycepin group injured with NaOH, treated with 20 mg/kg cordycepin; prednisolone group injured with NaOH, treated with 1 mg/kg prednisolone for 28 days. Efficacy was assessed by histopathological and immunohistochemical analysis of esophageal tissues. Results Cordycepin treatment significantly decreased inflammation, granulation tissue and fibrous tissue formation and prevented formation of esophageal strictures shown by histopathological damage score and stenosis indexes compared to control group (p 0.01). These effects are relatively more substantial than prednisolone, probably based on attenuation of elevation of proinflammatory cytokines hypoxia-inducible factor 1-alpha (HIF-1?), tumor necrosis factor alpha (TNF-?), proliferative and fibrotic factor fibroblast growth factor 2 (FGF2) and angiogenic factor vascular endothelial growth factor A (VEGFA) (p 0.05). Conclusions The findings suggest that cordycepin has a complex multifactorial healing process in alkali-burned tissue, more successful than prednisolone in preventing the formation of esophageal strictures and may be used as a therapeutic agent in the acute phase of esophageal alkali-burn.(AU)


Assuntos
Animais , Ratos , Estenose Esofágica/prevenção & controle , Estenose Esofágica/veterinária , Álcalis , Queimaduras/veterinária , Queimaduras/terapia
14.
Acta cir. bras. ; 35(6): e202000602, 2020. graf, tab
Artigo em Inglês | VETINDEX | ID: vti-30676

Resumo

Purpose To examine the effects of quercetin on healing of experimental colon anastomosis injury in early and late period. Methods Eighty male Wistar-Albino rats were divided into 8 groups. For all groups, left colons of the rats were resected and for the rest end-to-end anastomosis was performed. Two of the groups for which the experiment protocol was ended on the 3rd and 7th day following the anastomosis were not administered with either quercetin or dimethylsulfoxide DMSO, whereas two other groups were administered with DMSO only, and four other groups were administered with quercetin dissolved in DMSO in doses of 20 and 100 mg/kg during the protocol. At the end of the study, anastomosis line was resected, histopathological evaluation was performed and bursting pressure, malondialdehyde, superoxide dismutase, catalase, and hydroxyproline levels were measured. Results Quercetin significantly increased hydroxyproline, superoxide dismutase, catalase levels, histopathological healing score, bursting pressure values and decreased malondialdehyde level in early period. It also significantly increased superoxide dismutase, catalase, and hydroxyproline levels and decreased malondialdehyde level in late period. Conclusion It was seen that quercetin speeds up the injury healing process and reveals an antioxidant effect, specifically in early period.(AU)


Assuntos
Animais , Ratos , Ratos Wistar/metabolismo , Ratos Wistar/fisiologia , Quercetina/administração & dosagem , Quercetina/análise , Colo/lesões , Anastomose Cirúrgica
15.
Acta cir. bras. ; 35(4): e202000402, June 19, 2020. ilus, tab
Artigo em Inglês | VETINDEX | ID: vti-28735

Resumo

Purpose To investigate the effects of bradykinin on reperfusion injury in an experimental intestinal ischemia reperfusion model. Methods We used 32 Wistar-Albino rats. We composed 4 groups each containing 8 rats. Rats in sham group were sacrified at 100 minutes observation after laparotomy. Thirty minutes reperfusion was performed following 50 minutes ischaemia in control group after observing 20 minutes. Ischaemic preconditioning was performed in one group of the study. We performed the other study group pharmacologic preconditioning by infusional administration of 10 g/kg/minute bradykinin intravenously. We sacrified all of the rats by taking blood samples to evaluate the lactate and lactate dehydrogenase (LDH) after resection of jejunum for detecting tissue myeloperoxidase (MPO) activity. Results Lactate and LDH levels were significantly higher in control and study groups than the sham group (P 0.001). There is no difference between the study groups statistically. (P>0.05). The results were the same for MPO levels. Although definitive cell damage was determinated in the control group by hystopatological evaluation, the damage in the study groups observed was lower in different levels. However, there was no significant difference between the study groups statistically (P>0.05). Conclusion Either ischeamic preconditioning or pharmacologic preconditioning made by bradykinin reduced the ischemia reperfusion injury at jejunum.(AU)


Assuntos
Ratos , Bradicinina/uso terapêutico , Reperfusão/veterinária , Isquemia/veterinária , Ratos Wistar/lesões
16.
Ciênc. Anim. (Impr.) ; 30(04, Supl. 2): 175-179, 2020. tab
Artigo em Português | VETINDEX | ID: biblio-1472556

Resumo

The objective of this study was to evaluate the effect of the ethanol extract of Simarouba versicolor on reproductive parameters of Wistar rats (Rattus norvegicus): implantation index (%), resorption index (%), pre-implant loss, post-implant loss of the parents. pregnancy, the rats with approximately 90 days, weighing 160 to 250 g were divided into 4 groups (n=8), Control, Sv-EtOH 10, 20, 40mg/kg. The formulas used to evaluate reproductive parameters were following: IMPLEMENTATION INDEX = (number of implantations / number of corpus luteum)x100; REABSORPTION INDEX = (no resorption*/ no implants) x100* no resorptions = (no implants)-(nofetuses); PRE-IMPLANT LOSSES = (No. of corpus luteum - No. of implants / No. of corpus luteum) x100; POSTIMPLANT LOSSES = (number of implants - number of natives / number of implants) x100. In the analysis of the implantation index, resorption, pre-implant loss and post-implantation loss were not significant when compared to the control. These results indicate the absence of maternal toxicity for the investigated doses during the period of pre-implantation, implantation and full term pregnancy. However, further studies with different doses and concentrations of Simarouba versicolor extract will be necessary in the gestational period.


Assuntos
Feminino , Animais , Gravidez , Ratos , Prenhez/efeitos dos fármacos , Ratos Wistar/fisiologia , Simarouba/efeitos adversos , Simarouba/toxicidade
17.
Ci. Anim. ; 30(04, Supl. 2): 175-179, 2020. tab
Artigo em Português | VETINDEX | ID: vti-32339

Resumo

The objective of this study was to evaluate the effect of the ethanol extract of Simarouba versicolor on reproductive parameters of Wistar rats (Rattus norvegicus): implantation index (%), resorption index (%), pre-implant loss, post-implant loss of the parents. pregnancy, the rats with approximately 90 days, weighing 160 to 250 g were divided into 4 groups (n=8), Control, Sv-EtOH 10, 20, 40mg/kg. The formulas used to evaluate reproductive parameters were following: IMPLEMENTATION INDEX = (number of implantations / number of corpus luteum)x100; REABSORPTION INDEX = (no resorption*/ no implants) x100* no resorptions = (no implants)-(nofetuses); PRE-IMPLANT LOSSES = (No. of corpus luteum - No. of implants / No. of corpus luteum) x100; POSTIMPLANT LOSSES = (number of implants - number of natives / number of implants) x100. In the analysis of the implantation index, resorption, pre-implant loss and post-implantation loss were not significant when compared to the control. These results indicate the absence of maternal toxicity for the investigated doses during the period of pre-implantation, implantation and full term pregnancy. However, further studies with different doses and concentrations of Simarouba versicolor extract will be necessary in the gestational period.(AU)


Assuntos
Animais , Feminino , Gravidez , Ratos , Ratos Wistar/fisiologia , Prenhez/efeitos dos fármacos , Simarouba/efeitos adversos , Simarouba/toxicidade
18.
Acta cir. bras. ; 35(1): e202000103, Mar. 20, 2020. ilus, tab, graf
Artigo em Inglês | VETINDEX | ID: vti-25790

Resumo

Purpose To investigate the protective effect of Ganoderma lucidum on testicular torsion/detorsion (T/D)-induced ischemia-reperfusion (I/R) injury. Methods Thirty male Wistar albino rats were randomly categorized into 3 groups: Group 1: sham, Group 2 ( T/D): 2,5 hours of ischemia and 7 days of reperfusion, Group 3 (T/D+ G. lucidum ): 2,5 hours of ischemia and 7 days of reperfusion and 7 days of 20 mg/kg via gastric gavage G. lucidum polysaccharides per day. Biochemical assays of Malondialdehyde (MDA), superoxide dismutase (SOD), Catalase (CAT), Glutathione (GSH) levels , histopathology and expression levels of VEGF and Bcl-2 with immunohistochemical methods were examined in testicular tissue. Results G. lucidum treatment was found to have prevented the T/D-induced I/R injury by decreasing MDA levels of the testis. SOD, CAT and GSH activities were decreased in group 2, while they were increased in group 3 (p<0.001) and significant improvement in the tube diameter was observed in group 3. Bcl-2-positive germinal cells were lowered in group 3 compared to the group 2. VEGF expression showed an increase in group 2, whereas it decreased in group 3. Conclusion The antioxidant G. lucidum is thought to induce angiogenesis by reducing the apoptotic effect in testicular torsion-detorsion.(AU)


Assuntos
Animais , Masculino , Ratos , Reishi , Medicamentos de Ervas Chinesas/uso terapêutico , Traumatismo por Reperfusão/veterinária , Linfoma de Células B , Testículo , Torção do Cordão Espermático , Ratos Wistar , Fator A de Crescimento do Endotélio Vascular
19.
Ciênc. Anim. (Impr.) ; 30(04, Supl. 2): 185-189, 2020. tab
Artigo em Português | VETINDEX | ID: biblio-1472558

Resumo

The search for the ideal body has been making people appeal increasingly to surgical intervention and medicine which can accelerate the aesthetic transformations that are so desired. Among these medicines, Anabolic-androgenic steroids (AAS) is being used, specially Nandrolone Decanoate (DECA), which has more anabolic than androgenic effects. However, it is not known at which point of the use of AAS can comprise fertility of male users. In this way, the objective of the present study was to evaluate the effects of chronic administration of doses of Nandrolone Decanoate in male Wistar rat fertility. For such a matter was used 20 rats Wistar, which 16 were males and 4 females, adults, with 8 weeks old, weighing 250 to 350g. Males were divided into two groups: Control and DECA group. Based on the data preliminarily obtained, even though observing some macroscopics abnormalities in the testicles of rats treated with DECA, such as testicular atrophy, apparently these steroids do not affect the reproductive male health significatively at the point of comprising the capacity of fertilization.


Assuntos
Masculino , Animais , Ratos , Esteroides/administração & dosagem , Esteroides/toxicidade , Fertilidade/efeitos dos fármacos , Ratos Wistar
20.
Ci. Anim. ; 30(04, Supl. 2): 185-189, 2020. tab
Artigo em Português | VETINDEX | ID: vti-32338

Resumo

The search for the ideal body has been making people appeal increasingly to surgical intervention and medicine which can accelerate the aesthetic transformations that are so desired. Among these medicines, Anabolic-androgenic steroids (AAS) is being used, specially Nandrolone Decanoate (DECA), which has more anabolic than androgenic effects. However, it is not known at which point of the use of AAS can comprise fertility of male users. In this way, the objective of the present study was to evaluate the effects of chronic administration of doses of Nandrolone Decanoate in male Wistar rat fertility. For such a matter was used 20 rats Wistar, which 16 were males and 4 females, adults, with 8 weeks old, weighing 250 to 350g. Males were divided into two groups: Control and DECA group. Based on the data preliminarily obtained, even though observing some macroscopics abnormalities in the testicles of rats treated with DECA, such as testicular atrophy, apparently these steroids do not affect the reproductive male health significatively at the point of comprising the capacity of fertilization.(AU)


Assuntos
Animais , Masculino , Ratos , Ratos Wistar , Esteroides/administração & dosagem , Esteroides/toxicidade , Fertilidade/efeitos dos fármacos
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