Intake and apparent nutrient digestibility, ruminal parameters, and nitrogen balance of lambs fed with corn and forage-free diets with sodium bicarbonate / Consumo e digestibilidade aparente dos nutrientes, parâmetros ruminais e balanço de N de cordeiros confinados recebendo dietas com milho e sem forragem com bicarbonato de sódio

This study evaluated forage-free diets supplemented with sodium bicarbonate (SB) based on ground flint corn on intake, apparent nutrient digestibility, ruminal parameters, and N balance. Four rumen-cannulatedDorper × Santa Inês lambs, with a body weight of 43.4 ± 0.85 kg (mean ± standard deviation) were used. The experimental design was a 4×4 Latin square. Each experimental period lasted 21 days. Experimental diets were: positive control, a diet without SB containing 400 g/kg DM of soybean hulls (CONT), and three diets based on ground flint corn, containing 10 (10SB), 20 (20SB) or 30 (30SB) g/kg DM of SB. Intake of neutral and acid detergent fiber was greater for CONT (P < 0.01) when compared to SB. Intake and apparent nutrient digestibility of dry and organic matter, ether extract, and crude protein did not differ. Intake (P = 0.01) and digestibility (P < 0.01) of non-fiber carbohydrates were greater for SB diets and neutral detergent fiber digestibility was greater (P = 0.03) for CONT. The total digestible nutrients and metabolizable energy were greater for SB (P = 0.04). Rumen concentrations of acetate (P < 0.01), isobutyrate (P < 0.01), butyrate (P < 0.01), isovalerate (P < 0.01), and total short-chain fatty acids (P < 0.01) were greater for CONT. Ruminal pH showed a quadratic (P = 0.05) response to SB levels, with greater values for 20SB. Sodium bicarbonate was efficient in increasing ruminal pH at the level of 20 g/kg DM without interfering with the intake and apparent nutrient digestibility characteristics.

O presente estudo avaliou a suplementação de bicarbonato de sódio (BS) em dietas sem forragem à base de milho moído sobre o consumo e a digestibilidade aparente dos nutrientes, os parâmetros ruminais e o balanço de N. Foram utilizados quatro cordeiros Dorper × Santa Inês, com um peso corporal inicial de 43,4 ± 0,85 kg (média ± desvio padrão). O delineamento experimental foi quadrado latino 4×4. Cada período experimental durou 21 dias. As dietas experimentais foram: controle positivo, sem BS, contendo 400 g/kg de MS de casca de soja (CONT) e três dietas à base de milho moído, sem casca de soja, contendo 10 (10BS), 20 (20BS) ou 30 (30BS) g/kg de MS de BS. O consumo de fibra em detergente neutro e ácido foi maior (P < 0,01)para o CONT quando comparado com BS. O consumo e a digestibilidade aparente da matéria seca, matéria orgânica, extrato etéreo e proteína bruta não diferiram entre os tratamentos. O consumo (P = 0,01) e digestibilidade (P < 0,01) dos carboidratos não fibrosos foram superiores para as dietas contendo BS e a digestibilidade da fibra em detergente neutro foi maior para o CONT. Os teores de nutrientes digestivos totais e energia metabolizável foram superiores para as dietas contendo BS (P = 0,04). A concentração ruminal de acetato (P < 0,01), isobutirato (P < 0,01), butirato (P < 0,01), isovalerato (P < 0,01) e ácidos graxos totais de cadeia curta (P < 0,01) foram mais elevadas para o CONT. O pHruminal apresentou resposta quadrática (P = 0,05) em relação aos níveis de BS, sendo observado maior pH para os animais alimentados com a dieta 20BS. O BS foi eficiente em aumentar o pHruminal no teor de 20 g/kg MS sem interferir no consumo e na digestibilidade aparente dos nutrientes. Portanto, 20 g/kg de MS é a dose indicada de BS em dietas sem forragem para terminação de cordeiros em confinamento.

Meta-Analysis of Butyric Acid: a Performance-Enhancing Additive to Replace Antibiotics for Broiler Chickens

Butyric acid has been studied and utilized intensively in broiler chicken production in order to substitute growth-promoting antibiotics. However, the comprehensive literature on this topic makes it difficult to understand overall results, since there is a noticeable number of studies with conflicting conclusions. Although several research studies have shown that butyric acid may increment broiler chicken's performance, several other studies show the opposite. This work aimed to organize information to understand, through a meta-analysis, whether butyric acid can be used as a performance-enhancing additive for broiler chickens, and whether it can replace growth-promoting antibiotics. It was clear from the results that the effect of butyric acid depends on the microbiological challenge that broilers receive. When broilers are raised without health challenge, butyric acid and even antibiotics do not influence growth performance. However, when broilers are challenged, butyric acid provided an increase in weight gain and a significant improvement in feed conversion, matching the results of antibiotics. We conclude that butyric acid improves broiler performance and can be used to replace growth-promoting antibiotics.(AU)

Strategic grazing management decreases nitrogen excretion intensity of dairy cows

ABSTRACT: There is limited information regarding both nitrogen (N) and energy partitioning of dairy cows grazing wellmanaged tropical pastures. The objective of this study was to investigate the N and energy partitioning of midlactation dairy cows on rotationally grazed elephant grass using two pregrazing targets: 95 % or maximum canopy light interception (LI95% or LIMax) during regrowth. The study used 26 Holstein × Jersey dairy cows arranged in a randomized complete block design with three 40day periods of sampling. Grazing at LI95% increased organic matter and crude protein intake by 20 % (p 0.05) which resulted in a 9 % increase in fat corrected milk yield (p 0.05) relative to LIMax. Cows grazing at LI95% had greater concentration of total volatile fatty acids, butyrate and valerate (p 0.05), and smaller acetate (p 0.05) than those grazing at LIMax. Intake of net energy for lactation (NEL) and NEL secreted in milk were greater (p 0.05), while partitioning of NEL towards maintenance tended to be greater (p = 0.07) for cows grazing at LI95% than those grazing at LIMax. Milk urea nitrogen and both urine and fecal N excretion were greater for cows grazing at LI95% (p 0.05), but N excretion intensity was lower than in cows grazing at LIMax (p 0.05). Strategic grazing management using the LI95% pregrazing target increases N losses through both urine and feces; however, it reduces N excretion intensity of dairy cows by 9 %.

Fermentative profile of corn silages with the inclusion of chemical additives or bacterial inoculant / Perfil fermentativo de silagens de milho com a inclusão de aditivos químicos ou inoculante bacteriano

The objective was to evaluate the inclusion of chemical additives or bacterial inoculant in corn silage. The experimental design was completely randomized with five treatments: silage without additive; silage added with urea (3.0%); silage with limestone (3.0%); silage added with crystal sugar (3.0%); and silage with a bacterial inoculant. Five of them were opened during the fermentation process (15 days) to measure pH, and three were opened 60 days after ensiling to evaluate the ammonia nitrogen and organic acids content. For the pH at the end of the fermentation process, higher values were found for the limestone additive, followed by the silage with urea, which had higher participation of lactic acid, 19.06 ppm for limestone and 18.95 ppm for urea. Higher concentrations of acetic acid were observed in inoculant silages (18.49ppm) or silage without additive (18.46ppm). The ammonia nitrogen content was higher in the silage with urea (23.74mg dL-1), followed by the silage without additive (7.54mg dL-1), which also had the highest concentration of butyric acid (4.19ppm). The use of additives reduced the concentration of butyric acid in the silage. The bacterial inoculant was the most efficient in decreasing the pH of the ensiled material.

Objetivou-se avaliar a inclusão de aditivos químicos ou inoculante bacteriano na silagem de milho. O delineamento experimental foi inteiramente ao acaso, com cinco tratamentos: silagem sem aditivo; silagem aditivada com ureia (3,0%); silagem aditivada com calcário (3,0%); silagem aditivada com açúcar cristal (3,0%); e silagem com inoculante bacteriano. Cinco sacos de silagem foram abertos durante o processo fermentativo (15 dias) para mensuração de pH, e três 60 dias após a ensilagem, para avaliação do teor de nitrogênio amoniacal e de ácidos orgânicos. Para pH no final do processo fermentativo, maiores valores foram verificados para o aditivo calcário, seguido da silagem com ureia, os quais apresentaram maior participação de ácido lático, 19,06 ppm para calcário e 18,95 ppm para ureia. Maiores concentrações de ácido acético foram observadas nas silagens inoculante (18,49 ppm) ou sem aditivo (18,46 ppm). O teor de nitrogênio amoniacal foi superior na silagem com ureia (23,74mg dL '), seguida pela silagem sem aditivo (7,54mg dL), que também apresentou maior concentração de ácido butírico (4,19 ppm). O uso de aditivos reduziu a concentração de ácido butírico na silagem. O inoculante bacteriano foi mais eficiente que aditivos químicos no declínio do pH do material ensilado.

Presence of leaves and use of IBA in adventitious rooting, chlorophyll fluorescence, and root ontogeny of 'Fepagro C 13' and 'Trifoliata' cuttings / Presença de folhas e uso de AIB no enraizamento adventício, fluorescência da clorofila e ontogenia radicular em estacas de 'Fepagro C 13' e 'Trifoliata'

Cutting is proposed as an alternative method for the propagation of citrus rootstocks to obtain clonal material especially in varieties with a low rate of polyembryony and possibly reduce the seedling formation period. The aim of this study was to examine the influence of leaves and the application of indolebutyric acid (IBA) on adventitious rooting and chlorophyll fluorescence as well as to characterize root ontogeny in 'Fepagro C 13' and 'Trifoliata' citrus rootstock cuttings. Semi-hardwood cuttings were collected in December 2018 and subjected to the following treatments: presence or absence of leaves, with or without application of IBA (4000 mg L-1). Over 92 days in a greenhouse environment, leaf abscission percentage, cutting mortality, rooting percentage, and number of roots per cutting were evaluated. Additionally, initial (Fo), maximum (Fm), and variable (Fv) chlorophyll fluorescence, photochemical efficiency (Fv/Fm), and Fv/Fo ratio were analyzed. Tissue samples from the base of the cutting were collected for the anatomical study. The presence of leaves on 'Fepagro C 13' and 'Trifoliata' cuttings is essential for adventitious rooting. The use of auxin influences the photochemical efficiency of leaves on the cuttings. In 'Trifoliata' cuttings, adventitious roots originate from the vascular cambium.(AU)

A estaquia é proposta como método alternativo para a propagação de porta-enxertos de citros, visando a garantia de obtenção de material clonal, especialmente em variedades com baixa taxa poliembriônica, e uma possível redução no período para a obtenção das mudas. O objetivo deste estudo foi avaliar a influência das folhas e da aplicação de ácido indolbutírico (AIB) sobre o enraizamento adventício, fluorescência da clorofila e caracterizar a ontogenia radicular em estacas dos porta-enxertos citrangeiro 'Fepagro C 13', e 'Trifoliata'. Estacas semilenhosas foram coletadas em dezembro de 2018 e submetidas aos tratamentos: presença ou ausência de folhas com aplicação ou não de AIB (4000 mg L-1). Ao longo de 92 dias em casa de vegetação foram avaliados os percentuais de abscisão foliar, mortalidade de estacas, percentual de enraizamento e número de raízes por estaca. Também os dados da fluorescência inicial (Fo), máxima (Fm) e variável (Fv) da clorofila, a eficiência fotoquímica (Fv/Fm) e a razão Fv/Fo foram avaliados. Amostras de tecido da base da estaca foram coletadas para a realização do estudo anatômico. A presença das folhas nas estacas de 'Fepagro C 13' e 'Trifoliata' é essencial para o enraizamento adventício. O uso de auxina influencia a eficiência fotoquímica nas folhas das estacas. Em estacas de 'Trifoliata' as raízes adventícias originam-se a partir do câmbio vascular.(AU)

Strategic grazing management decreases nitrogen excretion intensity of dairy cows

There is limited information regarding both nitrogen (N) and energy partitioning of dairy cows grazing well–managed tropical pastures. The objective of this study was to investigate the N and energy partitioning of mid–lactation dairy cows on rotationally grazed elephant grass using two pre–grazing targets: 95 % or maximum canopy light interception (LI95% or LIMax) during regrowth. The study used 26 Holstein × Jersey dairy cows arranged in a randomized complete block design with three 40–day periods of sampling. Grazing at LI95% increased organic matter and crude protein intake by 20 % (p ≤ 0.05) which resulted in a 9 % increase in fat corrected milk yield (p ≤ 0.05) relative to LIMax. Cows grazing at LI95% had greater concentration of total volatile fatty acids, butyrate and valerate (p ≤ 0.05), and smaller acetate (p ≤ 0.05) than those grazing at LIMax. Intake of net energy for lactation (NEL) and NEL secreted in milk were greater (p ≤ 0.05), while partitioning of NEL towards maintenance tended to be greater (p = 0.07) for cows grazing at LI95% than those grazing at LIMax. Milk urea nitrogen and both urine and fecal N excretion were greater for cows grazing at LI95% (p ≤ 0.05), but N excretion intensity was lower than in cows grazing at LIMax (p ≤ 0.05). Strategic grazing management using the LI95% pre–grazing target increases N losses through both urine and feces; however, it reduces N excretion intensity of dairy cows by 9 %.

Exploring the rumen microbial community in Guizhou White goats at different ages

This study evaluated the changes in rumen microbiome during the process of age development of farming Guizhou White goats from Southwest China. We conducted high-throughput 16S rRNA gene sequencing to investigate the diversity, structure, and composition of goat rumen microbiota (RM) of 21 goats of different age groups (1, 6, and 12 months). We found that volatile fatty acids (i.e., acetate, propionate, and butyrate) fermented by microbes were found to increase significantly in the sixand one-month-old goats. Results of the genera abundance analysis showed that abundance of eight and seven taxa decreased in six- and one-month-old goats, respectively, compared with that in 12-month-old goats. Additionally, differences in six taxa in six-month-old goats and in one taxon in one-month-old goats were found. In addition, specific gut microbiome was found, which was significantly correlated with rumen fermentation parameters in Guizhou White goats. These results revealed the signature microbiota in RM during various developmental stages in goats raised in Southwest China and can also provide a guiding tool for evaluating rumen health of ruminants worldwide.(AU)

Strategic grazing management decreases nitrogen excretion intensity of dairy cows

There is limited information regarding both nitrogen (N) and energy partitioning of dairy cows grazing well­managed tropical pastures. The objective of this study was to investigate the N and energy partitioning of mid­lactation dairy cows on rotationally grazed elephant grass using two pre­grazing targets: 95 % or maximum canopy light interception (LI95% or LIMax) during regrowth. The study used 26 Holstein × Jersey dairy cows arranged in a randomized complete block design with three 40­day periods of sampling. Grazing at LI95% increased organic matter and crude protein intake by 20 % (p ≤ 0.05) which resulted in a 9 % increase in fat corrected milk yield (p ≤ 0.05) relative to LIMax. Cows grazing at LI95% had greater concentration of total volatile fatty acids, butyrate and valerate (p ≤ 0.05), and smaller acetate (p ≤ 0.05) than those grazing at LIMax. Intake of net energy for lactation (NEL) and NEL secreted in milk were greater (p ≤ 0.05), while partitioning of NEL towards maintenance tended to be greater (p = 0.07) for cows grazing at LI95% than those grazing at LIMax. Milk urea nitrogen and both urine and fecal N excretion were greater for cows grazing at LI95% (p ≤ 0.05), but N excretion intensity was lower than in cows grazing at LIMax (p ≤ 0.05). Strategic grazing management using the LI95% pre­grazing target increases N losses through both urine and feces; however, it reduces N excretion intensity of dairy cows by 9 %.

Chemical, mineral composition, in vitro ruminal fermentation and buffering capacity of some rangeland-medicinal plants

A diverse group of rangeland-medicinal plants are being used by ruminant whilst some of them have not been assessed for their nutritional value. This study was aimed to evaluate the chemical and mineral composition, buffering capacity, and in vitro fermentation of some rangeland-medicinal plants including Thymus kotschyanus, Ziziphora persica, Lallemantia royleana, and Scutellaria litwinowii in the family Lamiaceae, and Hypericum scabrum, in the family Hypericaceae. The results indicated that crude protein (CP) content ranged from 8.66% (S. litwinowii) to 12.17% of DM (H. scabrum). It was found that Z. persica had the highest potential gas production, metabolism energy (ME), relative feed value (RFV), and dry matter digestibility (DMD) values of 53.44 (mL 200-1 mg DM), 5.84 (MJ kg-1 DM), 170.66 and 70.88%, respectively. Mineral content differed among plants; Ca ranged from 5.79 to 41.96 g kg-1 DM. The concentrations of Ca, K, Mg, Fe, Zn, and Co were highest for L. royleana. Total volatile fatty acids (TVFA) and propionate concentrations were highest in the culture medium cultured with Z. persica, however, acetate, and butyrate were highest in H. scabrum. Acid-base buffering capacity was lower in T. kotschyanus and H. scabrum compared to other plants, while it was higher in S. litwinowii. Overall, it can be concluded that among plants evaluated in this study, Z. persica had higher nutritional value for sheep feeding.(AU)

Enzymatic synthesis optimization of isoamyl butyrate from fusel oil

Many food, cosmetic and pharmaceutical industries have increased their interest in short-chain esters due to their flavor properties. From the industrial standpoint, enzyme reactions are the most economical strategy to reach green products with neither toxicity nor damage to human health. Isoamyl butyrate (pear flavor) was synthesized by isoamyl alcohol (a byproduct of alcohol production) and butyric acid with the use of the immobilized lipase Lipozyme TL IM and hexane as solvents. Reaction variables (temperature, butyric acid concentration, isoamyl alcohol:butyric acid molar ratio and enzyme concentration) were investigated in ester conversion (%), concentration (mol L-1) and productivity (mmol ester g-1 mixture . h), by applying a sequential strategy of the Fractional Factorial Design (FFD) and the Central Composite Rotatable Design (CCRD). High isoamyl butyrate conversion of 95.8% was achieved at 24 hours. At 3 hours, the highest isoamyl butyrate concentration (1.64 mol L-1) and productivity (0.19 mmol ester g-1 mixture . h) were obtained under different reaction conditions. Due to high specificity and selectivity of lipases, process parameters of this study and their interaction with the Lipozyme TL IM are fundamental to understand and optimize the system so as to achieve maximum yield to scale up. Results show that fusel oil may be recycled by the green chemistry process proposed by this study.

Enzymatic synthesis optimization of isoamyl butyrate from fusel oil

Many food, cosmetic and pharmaceutical industries have increased their interest in short-chain esters due to their flavor properties. From the industrial standpoint, enzyme reactions are the most economical strategy to reach green products with neither toxicity nor damage to human health. Isoamyl butyrate (pear flavor) was synthesized by isoamyl alcohol (a byproduct of alcohol production) and butyric acid with the use of the immobilized lipase Lipozyme TL IM and hexane as solvents. Reaction variables (temperature, butyric acid concentration, isoamyl alcohol:butyric acid molar ratio and enzyme concentration) were investigated in ester conversion (%), concentration (mol L-1) and productivity (mmol ester g-1 mixture . h), by applying a sequential strategy of the Fractional Factorial Design (FFD) and the Central Composite Rotatable Design (CCRD). High isoamyl butyrate conversion of 95.8% was achieved at 24 hours. At 3 hours, the highest isoamyl butyrate concentration (1.64 mol L-1) and productivity (0.19 mmol ester g-1 mixture . h) were obtained under different reaction conditions. Due to high specificity and selectivity of lipases, process parameters of this study and their interaction with the Lipozyme TL IM are fundamental to understand and optimize the system so as to achieve maximum yield to scale up. Results show that fusel oil may be recycled by the green chemistry process proposed by this study.(AU)

Effect of Dietary Inclusion of Protected Sodium Butyrate on the Digestibility and Intestinal Histomorphometry of Commercial Laying Hens

ABSTRACT The objective of this study was to evaluate the effects of dietary inclusion of protected sodium butyrate (PSB) on the intestinal development and feed nutrient metabolizability of commercial laying hens. The birds started to receive the treatment rations at 58 weeks of age. At 76 weeks of age the laying hens were distributed in a randomized block design to four treatments (0, 105, 210 and 300 g t-1/PSB), six replicates, and two birds/replicate. The nitrogen balance (NB), ether extract balance (EEB), dry matter metabolizability coefficient, nitrogen, ether extract, ash, apparent metabolizable energy (AME), and AME corrected by nitrogen (AMEn) were evaluated. For assessment of intestinal development, we evaluated the duodenum, jejunum, ileum, cecum, and colorectal lengths, the relative intestine weight and villus height, crypt depth, and villus:crypt ratio of the duodenum, jejunum, and ileum. A decreasing linear effect was observed in the duodenum length, while an increasing linear effect was observed in the height of the duodenum and jejunum villi. A quadratic effect was found in the jejunum crypt depth. A linear increasing effect was found on the villus:crypt ratio of the duodenum and jejunum, and a quadratic effect was observed in the ileum. Quadratic and increasing linear effects were observed in the NB and EEB, respectively. Additionally, increasing linear effects were observed in the AME and AMEn. The dietary addition of 300 g t-1 of PSB improved intestinal development and energy metabolizability of the diet.

Sodium butyrate in post-hatch feed for broiler chicks / [Uso de butirato de sódio em rações pós-eclosão para pintos de corte]

This study was designed to determine adequate levels for sodium butyrate inclusion in pre-hatching and pre-starter feed in order to minimize the negative effects of post-hatch delayed placement on broiler chicks. Newly-hatched chicks were allotted in a completely randomized design, with five treatments, each comprising five replicates of ten birds each. Five dietetic levels of sodium butyrate (control, 0.050, 0.075, 0.100 and 0.200%) were used in the pre-starter feed offered to the chicks in the transporting box and during the pre-starter phase. Performance, yolk sac retraction, plasma glucose concentration, weight and histomorphometry of the small intestine were evaluated after 24 hours of feed access and at 7 days of age. A metabolic trial was performed when the chicks were seven to ten days of age. Supplementation of 0.1% sodium butyrate increased the development of broiler chicks' intestinal villi at seven days of age but was not able to improve performance in the pre-starter phase. Supplementation with sodium butyrate in a diet offered in the transportation box does not improve broiler performance in the pre-starter phase. Supplementation of up to 0.16% is recommended, in order to improve the metabolizability of the ether extract for broilers at seven days of age.(AU)

Objetivou-se, com este estudo, encontrar níveis adequados de inclusão de butirato de sódio em ração pós-eclosão e pré-inicial, buscando minimizar os efeitos negativos do jejum de pintos de corte. Os animais foram distribuídos em delineamento inteiramente ao acaso, com cinco tratamentos e cinco repetições de 10 aves cada. Foram utilizadas cinco suplementações de butirato de sódio (controle; 0,05; 0,075; 0,100 e 0,200%) na ração pré-inicial, fornecidas aos pintos na caixa de transporte e durante a fase pré-inicial. Após 24 horas de acesso ao alimento e aos sete dias de idade, foram avaliados desempenho, retração do saco vitelino, concentração de glicose plasmática, peso e histomorfometria do intestino delgado. De sete a 10 dias de idade, realizou-se ensaio de metabolizabilidade. A suplementação de 0,10% de butirato de sódio aumentou o desenvolvimento das vilosidades intestinais dos pintos de corte aos sete dias de idade, mas não foi capaz de melhorar o desempenho na fase pré-inicial. A suplementação com butirato de sódio em dieta oferecida na caixa de transporte não melhora o desempenho dos frangos na fase pré-inicial. Recomenda-se suplementação de até 0,16%, a fim de melhorar a metabolizabilidade do extrato etéreo para frangos de corte aos sete dias de idade.(AU)

Propagation of 'Bruno' kiwifruit: influence of cutting collection time and indolebutyric acid / Propagação de kiwizeiro 'Bruno': influência da época de coleta das estacas e ácido indolbutírico

In Brazil, the propagation of kiwifruit (Actinidia deliciosa) is achieved through seed-derived rootstocks, which results in low uniformity. An alternative to address this problem is the cuttings technique, a simple and fast method that generates individuals identical to the parent plant, maintaining the agronomic traits. This study was conducted to evaluate the effects of cutting collection time and indolebutyric acid (IBA) on the rooting of 'Bruno' kiwifruit cuttings. The cuttings were collected in the months of May, August and December 2016 and March 2017 and treated with 0, 1000, 2000 and 4000 mg L-1 IBA, as a hydroalcoholic solution, for seven seconds. After 90 days, the percentages of rooted and dead cuttings and cuttings that produced shoots; leaf retention; average length of the three largest roots; average number of primary roots per cutting; and reserve contents before and after rooting were evaluated. The treatments with IBA influenced the rooting of cuttings collected in May, which showed 36.07% rooting at the calculated IBA concentration of 2,320 mg L-1, and in December, which exhibited 57.59% rooting at the concentration of 4000 mg L-1. In the present experimental conditions, it is recommended to root cuttings of 'Bruno' kiwifruit collected in December with the application of 4000 mg L-1 IBA.(AU)

No Brasil, para a propagação de kiwizeiros (Actinidia deliciosa) são utilizados porta-enxertos oriundos de sementes, resultando em baixa uniformidade. Uma alternativa é a estaquia, por ser método simples, rápido, que gera indivíduos idênticos à planta matriz, mantendo as características agronômicas. O objetivo deste estudo foi avaliar a época de coleta das estacas e o efeito do ácido indolbutírico (AIB) sobre o enraizamento de estacas de kiwizeiro 'Bruno'. As estacas foram coletadas nos meses de maio, agosto e dezembro de 2016 e março de 2017; e tratadas com zero, 1.000, 2.000 e 4.000 mg L-1 de AIB, na forma de solução hidroalcoólica, por sete segundos. Após 90 dias, avaliou-se a percentagem de estacas enraizadas; mortas; brotadas; retenção foliar; comprimento médio das três maiores raízes; número médio de raízes primárias por estaca, além do conteúdo de reservas antes e após o enraizamento. Os tratamentos com AIB influenciam o enraizamento das estacas coletadas em maio, com 36,07% de enraizamento na concentração calculada de 2.320 mg L-1, e em dezembro, atingindo 57,59% de enraizamento na concentração de 4.000 mg L-1 de AIB. Nas condições do presente experimento recomenda-se o enraizamento de estacas de kiwizeiro 'Bruno' coletadas em dezembro, aplicando-se 4.000 mg L-1 de AIB.(AU)

Dry matter intake and ruminal fermentation parameters in goats fed sugarcane in replacement of corn silage

ABSTRACT The trial was conducted with the objective to evaluate fresh sugarcane as a replacement for corn silage on dry matter intake, ruminal parameters (pH, short chain fatty acids, and ammonia nitrogen concentration), and degradation kinetics of forages and concentrate in maintenance adult goats. Four nonlactating ruminally cannulated goats were used in a 4 × 4 Latin square design with the replacement of 0, 33, 67, and 100% of corn silage by sugarcane as the independent variable. The substitution of sugarcane for corn silage did not affect dry matter intake, daily weight gain, or ruminal pH. A quadratic effect was observed for ammonia nitrogen concentration with a maximum concentration at 74.43% of sugarcane inclusion, as well as for short chain fatty acids, with maximum concentrations of acetate and butyrate at 0.59 and 32.70% of sugarcane inclusion, respectively. The minimum concentration of propionate was verified on 10.36% of sugarcane inclusion. However, the acetate:propionate ratio linearly decreased with increased sugarcane content. No effect was observed of different rumen environments on forage dry matter and neutral detergent fiber degradability (effective and potential degradability, soluble and potential degradable fractions). Dry matter degradability was 66.79% for sugarcane and 72.62% for corn silage after 96 h of incubation. Neutral detergent fiber was 37.10 and 51.82% for sugarcane and corn silage, respectively, after 96 h of incubation. In conclusion, sugarcane can be used as a replacement for corn silage in maintenance adult goats feed without altering dry matter intake and forage degradability while increasing propionate production.

RESUMO O experimento foi conduzido para avaliar a cana-de-açúcar in natura em substituição à silagem de milho no consumo de matéria seca, parâmetros ruminais e cinética de degradação de forragens e concentrado em cabras adultas em mantença. Quatro cabras não lactantes canuladas no rúmen foram utilizadas em um delineamento em quadrado latino 4 × 4 com a substituição de 0, 33, 67 e 100% da silagem de milho por cana-de-açúcar. A substituição da cana-de-açúcar pela silagem de milho não afetou o consumo de matéria seca, o ganho de peso diário e o pH ruminal. Foi observado efeito quadrático para a concentração de nitrogênio amoniacal com concentração máxima de 74,43% de inclusão da cana, e também para ácidos graxos de cadeia curta, com concentrações máximas de acetato e butirato de 0,59 e 32,70% de inclusão da cana, respectivamente. A concentração mínima de propionato foi verificada em 10,36% de inclusão de cana-de-açúcar. No entanto, a relação acetato: propionato diminuiu linearmente com o aumento do teor de cana-de-açúcar. Não foi observado efeito de diferentes ambientes ruminais sobre a degradabilidade da matéria seca da forragem e da fibra em detergente neutro. A degradabilidade da matéria seca foi de 66,79% para a cana-de-açúcar e 72,62% para a silagem de milho após 96 h de incubação. A fibra em detergente neutro foi 37,10 e 51,82% para cana-de-açúcar e silagem de milho, respectivamente, após 96 h de incubação. Em conclusão, a cana-de-açúcar pode ser usada como substituto da silagem de milho na alimentação de cabras adultas de manutenção, sem alterar o consumo de matéria seca e a degradabilidade da forragem, aumentando a produção de propionato.

Indicators of immunosuppression peripartum in dual purpose cows in the tropics affected health, productive and reproductive parameters

O objetivo do estudo foi identificar indicadores de imunossupressão periparto em vacas de duplo propósito nos trópicos e determinar seus efeitos sobre parâmetros produtivos e reprodutivos. Os indicadores utilizados foram: alterações na população de leucócitos e neutrófilos, concentrações de metabólitos energéticos (β-hidroxibutirato e glicose) e escores de condição corporal (ECC). Amostras de sangue e BCS (escala 1 5) foram realizadas semanalmente durante o periparto. A saúde uterina foi avaliada (3 semanas após o parto) por ultrassonografia e usando um escore vaginal (0-3) descrito porSheldon et ai. (2006). Vacas (n=30) foram classificadas como endometrite saudável ou clínica (CE). A prevalência de EC foi de 29,6%. As populações de leucócitos e neutrófilos diminuíram no periparto e foram menores (P<0,05) nas vacas com CE. Vacas saudáveis ​​apresentaram maior (P<0,05) produção diária de leite do que aquelas com CE (18,84±0,63 vs 14,76±0,84 kg). Vacas CE tiveram desempenho reprodutivo inferior (P<0,05) em comparação com vacas saudáveis ​​(dias abertos: 244,40 ± 35,00 vs 178,00 ± 23,33 e serviços por concepção 3,33 ± 0,51 vs 1,83 ± 0,34). BCS similarmente (P>0,05) diminuiu após o parto em ambos os grupos. As concentrações de metabólitos energéticos durante o periparto flutuaram de maneira semelhante (P>0,05) em vacas saudáveis ​​e CE. Em resumo, vacas de duplo propósito em condições tropicais, apresentaram indicadores de imunossupressão periparto, caracterizada por um declínio na população de leucócitos, principalmente neutrófilos, bem como diminuição das concentrações de glicose e BCS pós-parto. Além disso, houve aumento nas concentrações de β-hidroxibutirato e vacas com CE tiveram efeito negativo nos parâmetros produtivos e reprodutivos.(AU)

Low doses of monensin for lambs fed diets containing high level of ground flint corn

Two experiments were proposed to evaluate the addition of monensin for lambs fed diets containing a high level of mature ground flint corn. The experimental diets were as follows: no inclusion of monensin (M0) and inclusion of 8 (M8), 16 (M16) and 24 mg kg-¹ of monensin (M24). In experiment 1, eight cannulated wethers were divided into a double 4 × 4 Latin square experimental design to evaluate nutrient digestibility, plasma parameters and rumen fermentation. The experiment lasted 112 days, divided into four periods of 28 days each. In experiment 2, ninety-two lambs were used in a randomized block design to evaluate the performance over 56 days. In experiment 1, doses of monensin had no effect on nutrient intake (p >= 0.07) and digestibility (p >= 0.09). There was a quadratic effect for acetate molar proportion (p = 0.01), acetate to propionate ratio (p = 0.04) and rumen pH (p < 0.01). However, there was no effect on the molar proportion of propionate and butyrate. The monensin decreased linearly the total SCFA concentration (p < 0.01). The inclusion of monensin increased glucose (p < 0.01) and decreased lactate concentration in plasma (p = 0.05). In experiment 2, monensin decreased dry matter intake (p = 0.04). However, there was a quadratic effect for average daily gain (p = 0.03) and feed efficiency (p < 0.01), with the greatest values observed for the M8 diet. Thus, the inclusion of 8 mg kg-¹ of dry matter diet (DM) improves ruminal fermentation and plasma parameters, resulting in greater growth performance in lambs.(AU)

Low doses of monensin for lambs fed diets containing high level of ground flint corn

Two experiments were proposed to evaluate the addition of monensin for lambs fed diets containing a high level of mature ground flint corn. The experimental diets were as follows: no inclusion of monensin (M0) and inclusion of 8 (M8), 16 (M16) and 24 mg kg-¹ of monensin (M24). In experiment 1, eight cannulated wethers were divided into a double 4 × 4 Latin square experimental design to evaluate nutrient digestibility, plasma parameters and rumen fermentation. The experiment lasted 112 days, divided into four periods of 28 days each. In experiment 2, ninety-two lambs were used in a randomized block design to evaluate the performance over 56 days. In experiment 1, doses of monensin had no effect on nutrient intake (p >= 0.07) and digestibility (p >= 0.09). There was a quadratic effect for acetate molar proportion (p = 0.01), acetate to propionate ratio (p = 0.04) and rumen pH (p < 0.01). However, there was no effect on the molar proportion of propionate and butyrate. The monensin decreased linearly the total SCFA concentration (p < 0.01). The inclusion of monensin increased glucose (p < 0.01) and decreased lactate concentration in plasma (p = 0.05). In experiment 2, monensin decreased dry matter intake (p = 0.04). However, there was a quadratic effect for average daily gain (p = 0.03) and feed efficiency (p < 0.01), with the greatest values observed for the M8 diet. Thus, the inclusion of 8 mg kg-¹ of dry matter diet (DM) improves ruminal fermentation and plasma parameters, resulting in greater growth performance in lambs.

Dry matter intake and ruminal fermentation parameters in goats fed sugarcane in replacement of corn silage / Parâmetros de ingestão de matéria seca e fermentação ruminal em cabras alimentadas com cana-de-açúcar em substituição à silagem de milho

The trial was conducted with the objective to evaluate fresh sugarcane as a replacement for corn silage on dry matter intake, ruminal parameters (pH, short chain fatty acids, and ammonia nitrogen concentration), and degradation kinetics of forages and concentrate in maintenance adult goats. Four nonlactating ruminally cannulated goats were used in a 4 × 4 Latin square design with the replacement of 0, 33, 67, and 100% of corn silage by sugarcane as the independent variable. The substitution of sugarcane for corn silage did not affect dry matter intake, daily weight gain, or ruminal pH. A quadratic effect was observed for ammonia nitrogen concentration with a maximum concentration at 74.43% of sugarcane inclusion, as well as for short chain fatty acids, with maximum concentrations of acetate and butyrate at 0.59 and 32.70% of sugarcane inclusion, respectively. The minimum concentration of propionate was verified on 10.36% of sugarcane inclusion. However, the acetate:propionate ratio linearly decreased with increased sugarcane content. No effect was observed of different rumen environments on forage dry matter and neutral detergent fiber degradability (effective and potential degradability, soluble and potential degradable fractions). Dry matter degradability was 66.79% for sugarcane and 72.62% for corn silage after 96 h of incubation. Neutral detergent fiber was 37.10 and 51.82% for sugarcane and corn silage, respectively, after 96 h of incubation. In conclusion, sugarcane can be used as a replacement for corn silage in maintenance adult goats feed without altering dry matter intake and forage degradability while increasing propionate production.(AU)

O experimento foi conduzido para avaliar a cana-de-açúcar in natura em substituição à silagem de milho no consumo de matéria seca, parâmetros ruminais e cinética de degradação de forragens e concentrado em cabras adultas em mantença. Quatro cabras não lactantes canuladas no rúmen foram utilizadas em um delineamento em quadrado latino 4 × 4 com a substituição de 0, 33, 67 e 100% da silagem de milho por cana-de-açúcar. A substituição da cana-de-açúcar pela silagem de milho não afetou o consumo de matéria seca, o ganho de peso diário e o pH ruminal. Foi observado efeito quadrático para a concentração de nitrogênio amoniacal com concentração máxima de 74,43% de inclusão da cana, e também para ácidos graxos de cadeia curta, com concentrações máximas de acetato e butirato de 0,59 e 32,70% de inclusão da cana, respectivamente. A concentração mínima de propionato foi verificada em 10,36% de inclusão de cana-de-açúcar. No entanto, a relação acetato: propionato diminuiu linearmente com o aumento do teor de cana-de-açúcar. Não foi observado efeito de diferentes ambientes ruminais sobre a degradabilidade da matéria seca da forragem e da fibra em detergente neutro. A degradabilidade da matéria seca foi de 66,79% para a cana-de-açúcar e 72,62% para a silagem de milho após 96 h de incubação. A fibra em detergente neutro foi 37,10 e 51,82% para cana-de-açúcar e silagem de milho, respectivamente, após 96 h de incubação. Em conclusão, a cana-de-açúcar pode ser usada como substituto da silagem de milho na alimentação de cabras adultas de manutenção, sem alterar o consumo de matéria seca e a degradabilidade da forragem, aumentando a produção de propionato.(AU)

Dry matter intake and ruminal fermentation parameters in goats fed sugarcane in replacement of corn silage / Parâmetros de ingestão de matéria seca e fermentação ruminal em cabras alimentadas com cana-de-açúcar em substituição à silagem de milho

The trial was conducted with the objective to evaluate fresh sugarcane as a replacement for corn silage on dry matter intake, ruminal parameters (pH, short chain fatty acids, and ammonia nitrogen concentration), and degradation kinetics of forages and concentrate in maintenance adult goats. Four nonlactating ruminally cannulated goats were used in a 4 × 4 Latin square design with the replacement of 0, 33, 67, and 100% of corn silage by sugarcane as the independent variable. The substitution of sugarcane for corn silage did not affect dry matter intake, daily weight gain, or ruminal pH. A quadratic effect was observed for ammonia nitrogen concentration with a maximum concentration at 74.43% of sugarcane inclusion, as well as for short chain fatty acids, with maximum concentrations of acetate and butyrate at 0.59 and 32.70% of sugarcane inclusion, respectively. The minimum concentration of propionate was verified on 10.36% of sugarcane inclusion. However, the acetate:propionate ratio linearly decreased with increased sugarcane content. No effect was observed of different rumen environments on forage dry matter and neutral detergent fiber degradability (effective and potential degradability, soluble and potential degradable fractions). Dry matter degradability was 66.79% for sugarcane and 72.62% for corn silage after 96 h of incubation. Neutral detergent fiber was 37.10 and 51.82% for sugarcane and corn silage, respectively, after 96 h of incubation. In conclusion, sugarcane can be used as a replacement for corn silage in maintenance adult goats feed without altering dry matter intake and forage degradability while increasing propionate production.

O experimento foi conduzido para avaliar a cana-de-açúcar in natura em substituição à silagem de milho no consumo de matéria seca, parâmetros ruminais e cinética de degradação de forragens e concentrado em cabras adultas em mantença. Quatro cabras não lactantes canuladas no rúmen foram utilizadas em um delineamento em quadrado latino 4 × 4 com a substituição de 0, 33, 67 e 100% da silagem de milho por cana-de-açúcar. A substituição da cana-de-açúcar pela silagem de milho não afetou o consumo de matéria seca, o ganho de peso diário e o pH ruminal. Foi observado efeito quadrático para a concentração de nitrogênio amoniacal com concentração máxima de 74,43% de inclusão da cana, e também para ácidos graxos de cadeia curta, com concentrações máximas de acetato e butirato de 0,59 e 32,70% de inclusão da cana, respectivamente. A concentração mínima de propionato foi verificada em 10,36% de inclusão de cana-de-açúcar. No entanto, a relação acetato: propionato diminuiu linearmente com o aumento do teor de cana-de-açúcar. Não foi observado efeito de diferentes ambientes ruminais sobre a degradabilidade da matéria seca da forragem e da fibra em detergente neutro. A degradabilidade da matéria seca foi de 66,79% para a cana-de-açúcar e 72,62% para a silagem de milho após 96 h de incubação. A fibra em detergente neutro foi 37,10 e 51,82% para cana-de-açúcar e silagem de milho, respectivamente, após 96 h de incubação. Em conclusão, a cana-de-açúcar pode ser usada como substituto da silagem de milho na alimentação de cabras adultas de manutenção, sem alterar o consumo de matéria seca e a degradabilidade da forragem, aumentando a produção de propionato.