Comparison of the effects of maropitant, lidocaine, and dextroketamine administered by the intratesticular route in dogs undergoing elective orchiectomy

Background: Balanced anesthesia achieved with combinations of inhaled and injectable drugs administered systemicallyor in loco-regional anesthetic blocks, is widely used in veterinary medicine. The use of anesthesia and/or local analgesiahas already demonstrated benefits in the performance of elective orchiectomy in different species, there is no literature thatevaluates the use of the maropitant intratesticular route. The present study evaluated the cardiorespiratory variables andanalgesia produced by intratesticular blockade with maropitant, lidocaine, or dextroketamine during the trans-operativeperiod along with the discharge and anesthetic recovery of dogs that underwent elective orchiectomy.Materials, Methods & Results: Used twenty-four dogs from routine elective orchiectomy, considered healthy based onthe results of clinical and hematological tests. The animals were randomly divided into three groups and was applied intratesticularly 2% lidocaine at a dose of 1 mg/kg (GL), 5% dextrocetamina at a dose of 2.5 mg/kg (GC), or 1% maropitantat a dose of 1 mg/kg (GM). Anesthesia induction was performed with propofol (to effect), and stabilization of inhalationalanesthesia was achieved with 1.7 V% of sevoflurane diluted in 100% oxygen administered through a calibrated vaporizerand appropriate anesthetic system based on the animal’s weight, being kept under spontaneous ventilation, After induction, we waited 10 min for stabilization of exhaled anesthetic concentration and then administered one of the treatmentsintratesticularly. After five min from the local block the surgical procedure was started during up to 15 min. Heart rate(HR), respiratory rate (RR), systolic arterial pressure (SAP), diastolic arterial pressure (DAP), mean arterial pressure(MAP), oxygen saturation of hemoglobin (SatO2), end-tidal carbon dioxide partial pressure (EtCO2), end-tidal sevofluraneconcentration...

Comparison of the effects of maropitant, lidocaine, and dextroketamine administered by the intratesticular route in dogs undergoing elective orchiectomy

Background: Balanced anesthesia achieved with combinations of inhaled and injectable drugs administered systemicallyor in loco-regional anesthetic blocks, is widely used in veterinary medicine. The use of anesthesia and/or local analgesiahas already demonstrated benefits in the performance of elective orchiectomy in different species, there is no literature thatevaluates the use of the maropitant intratesticular route. The present study evaluated the cardiorespiratory variables andanalgesia produced by intratesticular blockade with maropitant, lidocaine, or dextroketamine during the trans-operativeperiod along with the discharge and anesthetic recovery of dogs that underwent elective orchiectomy.Materials, Methods & Results: Used twenty-four dogs from routine elective orchiectomy, considered healthy based onthe results of clinical and hematological tests. The animals were randomly divided into three groups and was applied intratesticularly 2% lidocaine at a dose of 1 mg/kg (GL), 5% dextrocetamina at a dose of 2.5 mg/kg (GC), or 1% maropitantat a dose of 1 mg/kg (GM). Anesthesia induction was performed with propofol (to effect), and stabilization of inhalationalanesthesia was achieved with 1.7 V% of sevoflurane diluted in 100% oxygen administered through a calibrated vaporizerand appropriate anesthetic system based on the animals weight, being kept under spontaneous ventilation, After induction, we waited 10 min for stabilization of exhaled anesthetic concentration and then administered one of the treatmentsintratesticularly. After five min from the local block the surgical procedure was started during up to 15 min. Heart rate(HR), respiratory rate (RR), systolic arterial pressure (SAP), diastolic arterial pressure (DAP), mean arterial pressure(MAP), oxygen saturation of hemoglobin (SatO2), end-tidal carbon dioxide partial pressure (EtCO2), end-tidal sevofluraneconcentration...(AU)

Changes in the stocks of C and N in organic matter fractions in soil cropped with coffee and fertilized with sunn hemp and ammonium sulfate / Alterações dos estoques de C e N nas frações da matéria orgânica de solo cultivado com cafeeiro e adubado com crotalária e sulfato de amônio

Despite the potential to provide N to crops, the rapid incorporation of green manure nutrients into stable fractions of organic matter in the soil (SOM) may reduce the efficiency of green manuring. Thus, the objective of this work was to characterize the changes of C and N stocks in fractions of SOM cultivated with coffee (Coffea arabica L.) and fertilized with sunn hemp (Crotalaria juncea) and ammonium sulfate. To study the changes in organic C (OC) and total N (TN) in soil and fractions of SOM over time, soil samples were collected in the 0–5 and 5–10 cm layers, with the initial sampling done prior to the application of sunn hemp residues and ammonium sulfate. Five samples were collected every 2 months after the application of the legume and ammonium sulfate. The soil samples were submitted to densimetric and granulometric fractionation, obtaining the free light organic matter (F-LOM), particulate organic matter (POM), and organic matter associated with minerals (MAM). OC and TN stocks were then determined in soil and the SOM fractions. The changes in the stocks of OC (ΔStcC) in the soil in relation to time zero were positive in the evaluations carried out in the two layers. The fractions of SOM showed positive ΔStcC at almost all of the evaluated times. The N supplied to the soil in the form of mineral and organic fertilizer promoted an increase of 0.24 Mg ha-¹ of N in the 0–5 cm layer until after 60 days. Of this total, 0.03 Mg ha-¹ was associated with F-LOM, 0.07 Mg ha-¹ with POM, and the remainder was associated with MAM. Nearly 60% of the N that was supplied to the soil was drawn to the stable fractions of the SOM, indicating a rapid stabilization of this nutrient in the most recalcitrant organic compartments. Despite that, the variations in N stocks of MAM became smaller over time, and eventually became negative, in relation to time zero.[...](AU)

Apesar do potencial para fornecer N aos cultivos, a rápida incorporação dos nutrientes do adubo verde em frações estáveis da matéria orgânica do solo (MOS) pode reduzir a eficiência da adubação verde. Portanto, objetivou-se com este trabalho caracterizar as alterações temporais dos estoques de C e N em frações da matéria orgânica de solo cultivado com cafeeiro (Coffea arabica L.) e adubado com crotalária (Crotalaria juncea) e sulfato de amônio. Para estudar o comportamento do C orgânico (CO) e N total (NT) no solo e nas frações da MOS ao longo do tempo, foram coletadas amostras de solo nas camadas de 0–5 e 5–10 cm, sendo a primeira amostragem antes da aplicação dos resíduos de crotalária e sulfato de amônio. Outras cinco amostras foram coletadas a cada dois meses após a aplicação da leguminosa e sulfato de amônio. As amostras de solo foram submetidas ao fracionamento densimétrico e granulométrico, obtendo-se as frações matéria orgânica leve livre (MOLL), matéria orgânica particulada (MOP) e matéria orgânica associada aos minerais (MAM). Foram determinados os estoques de CO e NT no solo e nas frações da MOS. As variações nos estoques de C (ΔEstC) orgânico no solo, em relação ao tempo zero, foram positivas nas avaliações realizadas nas duas camadas. As frações da MOS apresentaram ΔEstC positivas em quase todos os tempos de avaliação. O N aportado ao solo na forma de fertilizante mineral e orgânico promoveu incremento de 0,24 Mg ha-¹ de N na camada de 0–5 cm até os 60 dias. Desse total, 0,03 Mg ha-¹ estavam associados à MOLL, 0,07 Mg ha-¹ à MOP e o restante estava associado à MAM. Quase 60% do N que foi aportado ao solo teve como destino frações estáveis da MOS, indicando rápida estabilização desse nutriente nos compartimentos orgânicos mais recalcitrantes. Apesar disso, com o passar do tempo as variações nos estoques de N da MAM tornaram-se menores, e posteriormente negativas, em relação ao tempo zero, indicando a mineralização do N desse compartimento.[...](AU)

Avaliação dos efeitos cardiorrespiratórios da dexmedetomidina isolada e associada à morfina em cadelas submetidas à ovariossalpingohisterectomia eletiva sob anestesia geral inalatória / Evaluation of cardiorespiratory effects of dexmedetomidine isolated and associated with morphine in bitches submitted to elective ovariossalpingohisterectomy under general inhalation anesthesia

Background: Elective ovariossalpingohysterectomy is one of the most accomplished surgeries in veterinary practice, presenting moderate degree of pain. Aiming at balanced anesthesia, α2-agonists have been increasingly used, with dexmedetomidine being characterized by its sympatholytic, sedative, analgesic properties and synergism with opioids, benzodiazepines and other drugs. The objective of this study was to evaluate the effects of dexmedetomidine alone and associated with morphine under the cardiovascular, respiratory and body temperature variables in the pre and trans-operative periods in bitches submitted to elective ovariossalpingohisterectomy under general inhalation anesthesia.Materials, Methods & Results: Sixteen bitches were used in this study, which were submitted to elective ovariossalpingohisterectomy, all of which were clinically healthy based on clinical and hematological results. The animals were randomized into two groups, in which intramuscular dexmedetomidine alone and 5 µg/kg (DG) and 0.3 mg/kg morphine (GDM). In both groups, anesthesia was induced with propofol administered and maintained with isoflurane diluted in 100% oxygen administered through a calibrated vaporizer and appropriate anesthetic system based on the weight of the animal and kept under spontaneous ventilation. After stabilization of the patient, the surgical procedure started, which lasted 45 min. The parameters evaluated were heart rate (HR), respiratory rate (ƒ), systolic blood pressure (SBP), diastolic blood pressure (DBP), mean arterial pressure (MAP), body temperature (TºC), expired isoflurane concentration , partial carbon dioxide gas pressure (ETCO2 ) and oxygen saturation in hemoglobin (SatO2 ), which were measured before and after the application of preanesthetic medication and at specific surgical times:[...]

Avaliação dos efeitos cardiorrespiratórios da dexmedetomidina isolada e associada à morfina em cadelas submetidas à ovariossalpingohisterectomia eletiva sob anestesia geral inalatória / Evaluation of cardiorespiratory effects of dexmedetomidine isolated and associated with morphine in bitches submitted to elective ovariossalpingohisterectomy under general inhalation anesthesia

Background: Elective ovariossalpingohysterectomy is one of the most accomplished surgeries in veterinary practice, presenting moderate degree of pain. Aiming at balanced anesthesia, α2-agonists have been increasingly used, with dexmedetomidine being characterized by its sympatholytic, sedative, analgesic properties and synergism with opioids, benzodiazepines and other drugs. The objective of this study was to evaluate the effects of dexmedetomidine alone and associated with morphine under the cardiovascular, respiratory and body temperature variables in the pre and trans-operative periods in bitches submitted to elective ovariossalpingohisterectomy under general inhalation anesthesia.Materials, Methods & Results: Sixteen bitches were used in this study, which were submitted to elective ovariossalpingohisterectomy, all of which were clinically healthy based on clinical and hematological results. The animals were randomized into two groups, in which intramuscular dexmedetomidine alone and 5 µg/kg (DG) and 0.3 mg/kg morphine (GDM). In both groups, anesthesia was induced with propofol administered and maintained with isoflurane diluted in 100% oxygen administered through a calibrated vaporizer and appropriate anesthetic system based on the weight of the animal and kept under spontaneous ventilation. After stabilization of the patient, the surgical procedure started, which lasted 45 min. The parameters evaluated were heart rate (HR), respiratory rate (ƒ), systolic blood pressure (SBP), diastolic blood pressure (DBP), mean arterial pressure (MAP), body temperature (TºC), expired isoflurane concentration , partial carbon dioxide gas pressure (ETCO2 ) and oxygen saturation in hemoglobin (SatO2 ), which were measured before and after the application of preanesthetic medication and at specific surgical times:[...](AU)

Arterial, Mixed Venous or Central Venous Hemogasometry and End Tidal CO2 in Dogs under Different Hemodynamic States

Background: Hemogasometric analysis is used in the interpretation of acid-base balance (ABB) and to access pulmonary ventilation. Already mixed venous oxygen saturation obtained at pulmonary artery correlates with tissue oxygenation. However, both samples can be difficult to access because of the difficulties in arterial and pulmonary catheterization. The aim of this study was to evaluate the feasibility of replacing the arterial and mixed venous bloods, the end tidal pressure of carbon dioxide (EtCO2) and central venous blood in the analysis of pulmonary ventilation, tissue oxygenation and ABB in dogs under different hemodynamic states.Material, Methods & Results: Nine dogs were used with an average weight of 19.6 ± 1.3 kg, anesthetized with isoflurane at 1.4 V% diluted on oxygen 60% (Baseline), and subsequently undergoing mechanical ventilation (MV) and the hypodinamic state (Hypo) with isoflurane at 3.5V% and mean arterial pressure (MAP) lower than 50 mmHg and hyperdynamic state (Hyper) by dobutamine infusion at 5 g/kg/min and with MAP 30% higher than baseline. For each time allowed a 15 min of stabilization by each hemodynamic status. Simultaneously were collected samples of 0.6 mL of arterial blood by metatarsal artery, mixed and central venous blood by pulmonary artery and right atrium for hemogasometric analysis. To access lung function we correlated and compared th

Arterial, mixed venous or central venous hemogasometry and end tidal CO2 in dogs under different hemodynamic states

Background: Hemogasometric analysis is used in the interpretation of acid-base balance (ABB) and to access pulmonary ventilation. Already mixed venous oxygen saturation obtained at pulmonary artery correlates with tissue oxygenation. However, both samples can be difficult to access because of the difficulties in arterial and pulmonary catheterization. The aim of this study was to evaluate the feasibility of replacing the arterial and mixed venous bloods, the end tidal pressure of carbon dioxide (EtCO2 ) and central venous blood in the analysis of pulmonary ventilation, tissue oxygenation and ABB in dogs under different hemodynamic states. Material, Methods & Results: Nine dogs were used with an average weight of 19.6 ± 1.3 kg, anesthetized with isoflurane at 1.4 V% diluted on oxygen 60% (Baseline), and subsequently undergoing mechanical ventilation (MV) and the hypodinamic state (Hypo) with isoflurane at 3.5V% and mean arterial pressure (MAP) lower than 50 mmHg and hyperdynamic state (Hyper) by dobutamine infusion at 5 μg/kg/min and with MAP 30% higher than baseline. For each time allowed a 15 min of stabilization by each hemodynamic status. Simultaneously were collected samples of 0.6 mL of arterial blood by metatarsal artery, mixed and central venous blood by pulmonary artery and right atrium for hemogasometric analysis. To access lung function we correlated and compared the EtCO2 values obtained by gas analyzer with expired carbon dioxide pressure in the arterial blood (PaCO2 ), mixed venous blood (PmvCO2 ) and central venous blood (PcvCO2 ). For the interpretation of tissue oxygenation we correlated and compared the values of mixed (SmvO2 ) and central (ScvO2 ) venous oxygen saturation. For the acid-base balance we used the correlation of potencial hydrogen (pH); carbon dioxide pressure (PCO2 ); bicarbonate ion (HCO3-); base excess (BE); […](AU)

Arterial, mixed venous or central venous hemogasometry and end tidal CO2 in dogs under different hemodynamic states

Background: Hemogasometric analysis is used in the interpretation of acid-base balance (ABB) and to access pulmonary ventilation. Already mixed venous oxygen saturation obtained at pulmonary artery correlates with tissue oxygenation. However, both samples can be difficult to access because of the difficulties in arterial and pulmonary catheterization. The aim of this study was to evaluate the feasibility of replacing the arterial and mixed venous bloods, the end tidal pressure of carbon dioxide (EtCO2 ) and central venous blood in the analysis of pulmonary ventilation, tissue oxygenation and ABB in dogs under different hemodynamic states. Material, Methods & Results: Nine dogs were used with an average weight of 19.6 ± 1.3 kg, anesthetized with isoflurane at 1.4 V% diluted on oxygen 60% (Baseline), and subsequently undergoing mechanical ventilation (MV) and the hypodinamic state (Hypo) with isoflurane at 3.5V% and mean arterial pressure (MAP) lower than 50 mmHg and hyperdynamic state (Hyper) by dobutamine infusion at 5 μg/kg/min and with MAP 30% higher than baseline. For each time allowed a 15 min of stabilization by each hemodynamic status. Simultaneously were collected samples of 0.6 mL of arterial blood by metatarsal artery, mixed and central venous blood by pulmonary artery and right atrium for hemogasometric analysis. To access lung function we correlated and compared the EtCO2 values obtained by gas analyzer with expired carbon dioxide pressure in the arterial blood (PaCO2 ), mixed venous blood (PmvCO2 ) and central venous blood (PcvCO2 ). For the interpretation of tissue oxygenation we correlated and compared the values of mixed (SmvO2 ) and central (ScvO2 ) venous oxygen saturation. For the acid-base balance we used the correlation of potencial hydrogen (pH); carbon dioxide pressure (PCO2 ); bicarbonate ion (HCO3-); base excess (BE); […]

INFLUÊNCIA DA INOCULAÇÃO E TRITURAÇÃO NA COMPOSTAGEM DE BIOMASSAS DE CARCAÇAS DE AVES POEDEIRAS

Mesmo a compostagem sendo uma técnica extensivamente estudada, diversos fatores continuam dificultando a sua eficiência e aplicabilidade. Adicionalmente, não há numerosos estudos com carcaças de aves poedeiras, em escala real, utilizando inoculação e trituração como parte dos tratamentos. Desta forma, os objetivos deste trabalho foram: Avaliar, em termos físico-químicos e microbiológicos, a eficiência de diferentes condições de compostagem quanto a degradação e estabilização de diferentes quantidades de carcaças de aves poedeiras, considerando inoculação específica e trituração mecânica facilitadores do processo. Foram utilizadas 600 aves, sendo 40 em cada tratamento contendo aves inteiras (tratamentos 1 e 2, sem e com inoculação bacteriana, respetivamente) e 60 nos tratamentos com aves trituradas (tratamentos 3 e 4, com e sem inoculação bacteriana, respectivamente). Cada tratamento continha 115 kg de maravalha úmida (55%), e aproximadamente 75 kg (T1 e T2) e 110 kg (T3 e T4) de carcaças. As células (alvenaria, 1 m3), foram dispostas em ambiente protegido com tela e coberto. Temperatura, umidade, pH e atividade microbiológica (hidrólise do diacetato de fluoresceína - DFA), foram mensurados semanalmente. Mensalmente, outros parâmetros foram avaliados: Coliformes Escherichia coli, Coliformes Totais, Clostridium perfrigens e Salmonella spp., teor de cinzas e matéria seca, bem como carbono e nitrogênio total e alguns nutrientes (fósforo, magnésio, cálcio, potássio, cobalto, cobre, ferro e manganês). Foi identificado que os tratamentos T3 e T4 apresentaram uma taxa de decomposição mais elevada que T1 e T2, considerando o comportamento da temperatura das leiras. Todos os tratamentos arrefecem cerca de 0,5 °C/dia, conforme dados da linearização das temperaturas na fase termofílica dos tratamentos. Neste mesmo período, T3 e T4 mantiveram-se 5 °C acima de T1 e T2, respectivamente. T3 atingiu a maturidade antes dos demais tratamentos, seguido por T4 e T2, conforme resultados de temperatura e relação C/N. A trituração mostrou-se conveniente para a compostagem de carcaças de animais, mas o processo de aeração das células precisa ser revisado, sendo limitante para o sucesso do tratamento. Ao final (162 dias), os compostos foram analisados com base em alguns parâmetros da Instrução Normativa (IN) n° 27/2006, alterada pela IN 7/2016 (ANEXO V) e IN 25/2006 do Ministério da Agricultura, Pecuária e Abastecimento, sendo que a presença de microrganismos inviabiliza o atendimento da legislação vigente relacionada ao registro de fertilizantes.

Even though composting is an extensively studied technique, several factors continue to hinder its efficiency and applicability. In addition, there are not many studies with carcasses of laying hens, in full scale, using inoculation and crushing as part of the treatments. Thus, the objectives of this work were: To evaluate, in physical-chemical and microbiological terms, the efficiency of different composting conditions regarding the degradation and stabilization of different quantities of carcasses of laying hens, considering specific inoculation and mechanical crushing to facilitate the process. 600 hens were used, 40 in each treatment containing whole hens (treatments 1 and 2, without and with bacterial inoculation, respectively) and 60 in treatments with mechanical crushed hens (treatments 3 and 4, with and without bacterial inoculation, respectively). Each treatment contained 115 kg of wet wood shavings (55%), and approximately 75 kg (T1 and T2) and 110 kg (T3 and T4) of carcasses. The cells (masonry, 1 m3), were placed in a screen-protected and covered environment. Temperature, humidity, pH and microbiological activity (hydrolysis of fluorescein diacetate - FDA) were measured weekly. Monthly, other parameters were evaluated: Coliforms Escherichia coli, Total Coliforms, Clostridium perfrigens and Salmonella spp., ash content and dry matter content, as well as total carbon and nitrogen and some nutrients (phosphorus, magnesium, calcium, potassium, cobalt, copper, iron and manganese). It was identified that treatments T3 and T4 presented a higher decomposition rate than T1 and T2, considering the behavior of windrow temperatures. All treatments cool about 0.5 ° C / day, according to the temperature linearization data in the thermophilic phase of the treatments. In the same period, T3 and T4 remained 5 ° C above T1 and T2, respectively. T3 reached maturity before the other treatments, followed by T4 and T2, according to temperature and C / N ratio results. The mechanical crushing proved to be convenient for the composting of animal carcasses, but the process of aeration of the cells needs to be revised, that limiting the success of the treatment. At the end (162 days), the compounds were analyzed based on some parameters of Normative Instruction (IN) No. 27/2006, as amended by IN 7/2016 (ANNEX V) and IN 25/2006 of the Ministry of Agriculture, Cattle and Supplying, and the presence of microorganisms makes it impossible to comply with current legislation related to the registration of fertilizers.

Carbon sequestration in clay and silt fractions of Brazilian soils under conventional and no-tillage systems

The capacity of soils to sequestrate carbon (C) is mainly related to the formation of organo-mineral complexes. In this study, we investigated the influence of soil management systems on the C retention capacity of soil with an emphasis on the silt and clay fractions of two subtropical soils with different mineralogy and climate. Samples from a Humic Hapludox and a Rhodic Hapludox, clayey soils cultivated for approximately 30 years under no-tillage (NT) and conventional tillage (CT) were collected from six layers distributed within 100-cm soil depth from each site and from an adjacent native forest. After the removal of particulate organic matter (POM), the suspension ( 53 µm) was sonicated, the silt and clay fractions were separated in accordance with Stokes' law and the carbon content of whole soil and physical fractions was determined. In the Humic Hapludox, the clay and silt fractions under NT showed a higher maximum C retention (72 and 52 g kg-1, respectively) in comparison to those under CT (54 and 38 g kg-1, respectively). Moreover, the C concentration increase in both fractions under NT occurred mainly in the topsoil (up to 5 cm). The C retention in physical fractions of Rhodic Hapludox varied from 25 to 32 g kg-1, and no difference was observed whether under an NT or a CT management system. The predominance of goethite and gibbsite in the Humic Hapludox, as well as its exposure to a colder climate, may have contributed to its greater C retention capacity. In addition to the organo-mineral interaction, a mechanism of organic matter self-assemblage, enhanced by longer periods of soil non-disturbance, seems to have contributed to the carbon stabilization in both soils.

Carbon sequestration in clay and silt fractions of Brazilian soils under conventional and no-tillage systems

The capacity of soils to sequestrate carbon (C) is mainly related to the formation of organo-mineral complexes. In this study, we investigated the influence of soil management systems on the C retention capacity of soil with an emphasis on the silt and clay fractions of two subtropical soils with different mineralogy and climate. Samples from a Humic Hapludox and a Rhodic Hapludox, clayey soils cultivated for approximately 30 years under no-tillage (NT) and conventional tillage (CT) were collected from six layers distributed within 100-cm soil depth from each site and from an adjacent native forest. After the removal of particulate organic matter (POM), the suspension ( 53 µm) was sonicated, the silt and clay fractions were separated in accordance with Stokes' law and the carbon content of whole soil and physical fractions was determined. In the Humic Hapludox, the clay and silt fractions under NT showed a higher maximum C retention (72 and 52 g kg-1, respectively) in comparison to those under CT (54 and 38 g kg-1, respectively). Moreover, the C concentration increase in both fractions under NT occurred mainly in the topsoil (up to 5 cm). The C retention in physical fractions of Rhodic Hapludox varied from 25 to 32 g kg-1, and no difference was observed whether under an NT or a CT management system. The predominance of goethite and gibbsite in the Humic Hapludox, as well as its exposure to a colder climate, may have contributed to its greater C retention capacity. In addition to the organo-mineral interaction, a mechanism of organic matter self-assemblage, enhanced by longer periods of soil non-disturbance, seems to have contributed to the carbon stabilization in both soils.(AU)

Uso da técnica TightRope modificada em cães com ruptura do ligamento cruzado cranial / Use of modification TightRope technique in dogs with rupture of cranial crucial ligament

A ruptura do ligamento cruzado cranial (RLCCr) figura entre as principais enfermidades ortopédicas na espécie canina, sendo responsável por instabilidade da articulação do joelho, o que resulta em claudicação e desenvolvimento de doença articular degenerativa (DAD). O objetivo deste trabalho foi avaliar os resultados da técnica de TightRope modificada (TRM) na estabilização do joelho de cães com ruptura do ligamento cruzado cranial (RLCCr). O procedimento cirúrgico foi realizado em seis animais, os quais foram avaliados por 120 dias de pós-operatório. A modificação da técnica para estabilização extra capsular da RLCCr, consiste na utilização de fio de fluorcarbono, e fio de aço inoxidável de 1 mm como forma de fixação óssea. A TRM promoveu a estabilidade do joelho dos pacientes com ruptura do ligamento cruzado cranial no pós-operatório imediato e durante o acompanhamento clínico pós-cirúrgico demonstrou, aos 30 dias, progressiva melhora da marcha e suporte de peso à deambulação, estando todos os animais recuperados clinicamente aos 120 dias de pós-operatório.(AU)

Rupture of the cranial cruciate ligament is one of the leading orthopedic diseases in dogs. Thus aimed to evaluate the techniqueof “Tightrope” modified (TRM) in the knees of dogs with the purpose of providing less invasive and cost-effective surgery. Thesurgical procedure was performed in six animals, and these were evaluated up to 120 days postoperatively. The modification of thetechnique for stabilization of extra capsular CCL consists in using fluorocarbon and the stainless steel wire of 1 mm in order bonefixation. The “TRM” promoted the stability of the knee of patients diagnosed with cranial cruciate ligament rupture in the immediatepostoperative period. Clinical follow-up after surgery showed after 30 days postoperatively, improved gait and progressive weightsupport for ambulation, being all animals recovered clinically at 120 days postoperatively.(AU)

Uso da técnica TightRope modificada em cães com ruptura do ligamento cruzado cranial / Use of modification TightRope technique in dogs with rupture of cranial crucial ligament

A ruptura do ligamento cruzado cranial (RLCCr) figura entre as principais enfermidades ortopédicas na espécie canina, sendo responsável por instabilidade da articulação do joelho, o que resulta em claudicação e desenvolvimento de doença articular degenerativa (DAD). O objetivo deste trabalho foi avaliar os resultados da técnica de TightRope modificada (TRM) na estabilização do joelho de cães com ruptura do ligamento cruzado cranial (RLCCr). O procedimento cirúrgico foi realizado em seis animais, os quais foram avaliados por 120 dias de pós-operatório. A modificação da técnica para estabilização extra capsular da RLCCr, consiste na utilização de fio de fluorcarbono, e fio de aço inoxidável de 1 mm como forma de fixação óssea. A TRM promoveu a estabilidade do joelho dos pacientes com ruptura do ligamento cruzado cranial no pós-operatório imediato e durante o acompanhamento clínico pós-cirúrgico demonstrou, aos 30 dias, progressiva melhora da marcha e suporte de peso à deambulação, estando todos os animais recuperados clinicamente aos 120 dias de pós-operatório.

Rupture of the cranial cruciate ligament is one of the leading orthopedic diseases in dogs. Thus aimed to evaluate the techniqueof “Tightrope” modified (TRM) in the knees of dogs with the purpose of providing less invasive and cost-effective surgery. Thesurgical procedure was performed in six animals, and these were evaluated up to 120 days postoperatively. The modification of thetechnique for stabilization of extra capsular CCL consists in using fluorocarbon and the stainless steel wire of 1 mm in order bonefixation. The “TRM” promoted the stability of the knee of patients diagnosed with cranial cruciate ligament rupture in the immediatepostoperative period. Clinical follow-up after surgery showed after 30 days postoperatively, improved gait and progressive weightsupport for ambulation, being all animals recovered clinically at 120 days postoperatively.

Texture and organic carbon contents do not impact amount of carbon protected in Malagasy soils

Soil organic carbon (SOC) is usually said to be well correlated with soil texture and soil aggregation. These relations generally suggest a physical and physicochemical protection of SOC within soil aggregates and on soil fine particles, respectively. Because there are few experimental evidences of these relations on tropical soils, we tested the relations of soil variables (SOC and soil aggregate contents, and soil texture) with the amount of SOC physically protected in aggregates on a set of 15 Malagasy soils. The soil texture, the SOC and water stable macroaggregate (MA) contents and the amount of SOC physically protected inside aggregates, calculated as the difference of C mineralized by crushed and intact aggregates, were characterized. The relation between these variables was established. SOC content was significantly correlated with soil texture (clay+fine silt fraction) and with soil MA amount while protected SOC content was not correlated with soil MA amount. This lack of correlation might be attributed to the highest importance of physicochemical protection of SOC which is demonstrated by the positive relation between SOC and clay+fine silt fraction.

Texture and organic carbon contents do not impact amount of carbon protected in Malagasy soils

Soil organic carbon (SOC) is usually said to be well correlated with soil texture and soil aggregation. These relations generally suggest a physical and physicochemical protection of SOC within soil aggregates and on soil fine particles, respectively. Because there are few experimental evidences of these relations on tropical soils, we tested the relations of soil variables (SOC and soil aggregate contents, and soil texture) with the amount of SOC physically protected in aggregates on a set of 15 Malagasy soils. The soil texture, the SOC and water stable macroaggregate (MA) contents and the amount of SOC physically protected inside aggregates, calculated as the difference of C mineralized by crushed and intact aggregates, were characterized. The relation between these variables was established. SOC content was significantly correlated with soil texture (clay+fine silt fraction) and with soil MA amount while protected SOC content was not correlated with soil MA amount. This lack of correlation might be attributed to the highest importance of physicochemical protection of SOC which is demonstrated by the positive relation between SOC and clay+fine silt fraction.

Fracionamento químico e físico da matéria orgânica de um argissolo vermelho sob diferentes sistemas de uso

The objective of this study was to evaluate the total organic carbon and the physical and chemical fractions of organic matter in an Alfisol under different use systems. The land use systems evaluated was homogeneous forestry of Eucalyptus grandis, agrosilvopastoral system and native grassland, in layers from 0.000-0.025 and 0.025-0.075m depth. The coarse fraction (CFG) and the carbon associated minerals (CAM) were obtained by physical fractionation. The free light fraction (FLL), light occluded (FLO) and heavy (FP) were obtained by densimetric physical fractionation. The non-humicfractions (NH), fulvic acid (AF), humic acid (HA) and humin (HU) were obtained by chemical fractionation. The Eucalyptus grandis system promoted higher total organic carbon, CFG, FLL and FLO and AF. In the surface layer, the protective recalcitrance mechanisms by organic matter molecular and chemical stabilization are overlapping stability which result from aggregates occlusion. As is difficult to form aggregates due to sandy soil matrix, carbon youth which enter the system is decomposed by microorganisms going to a more advanced stage of decomposition, forming in this case associations with silt and clay particles. This occurs in soils where the clay percentage is low. Through the fractionation it was observed that most of the soil organic matter is stored in the form of HU.

Objetivou-se com este estudo avaliar o carbono orgânico total e as frações físicas e químicas da matéria orgânica (MO) do solo em um Argissolo Vermelho Eutrófico arênico, submetido a diferentes sistemas de uso. Os sistemas de uso do solo avaliados foram: florestamento homogêneo de Eucalyptus grandis (EUC), sistema agrossilvipastoril na faixa (ASP) e campo nativo (CN), nas camadas de 0,000-0,025m e de 0,025-0,075m. A fração grosseira (CFG) e o carbono associado aos minerais (CAM) foram obtidos por meio de fracionamento físico granulométrico. As frações leve livre (FLL), leve oclusa (FLO) e pesada (FP) foram obtidas por meio de fracionamento físico densimétrico. As frações não húmicas (NH), ácido fúlvico (AF), ácido húmico (AH) e humina (HU) foram obtidas por meio de fracionamento químico. O sistema EUC promoveu maiores estoques de carbono orgânico total, CFG, FLL e FLO e na fração AF nas camadas avaliadas. Na camada superficial, os mecanismos de proteção da MO por recalcitrância molecular e estabilização química estão sobrepondo a estabilidade decorrente da oclusão em agregados. Com a dificuldade de formar agregados, devido a matrizes arenosas desse solo, o carbono jovem que entra no sistema é decomposto pelos microrganismos, entrando em um estágio mais avançado de decomposição e, nesse caso, formando associações com as partículas silte e argila, mesmo em solos em que o percentual de argila é baixo. Através do fracionamento químico, observou-se que a maior parte da MO do solo encontra-se armazenada na forma de HU.

Fracionamento químico e físico da matéria orgânica de um argissolo vermelho sob diferentes sistemas de uso

The objective of this study was to evaluate the total organic carbon and the physical and chemical fractions of organic matter in an Alfisol under different use systems. The land use systems evaluated was homogeneous forestry of Eucalyptus grandis, agrosilvopastoral system and native grassland, in layers from 0.000-0.025 and 0.025-0.075m depth. The coarse fraction (CFG) and the carbon associated minerals (CAM) were obtained by physical fractionation. The free light fraction (FLL), light occluded (FLO) and heavy (FP) were obtained by densimetric physical fractionation. The non-humicfractions (NH), fulvic acid (AF), humic acid (HA) and humin (HU) were obtained by chemical fractionation. The Eucalyptus grandis system promoted higher total organic carbon, CFG, FLL and FLO and AF. In the surface layer, the protective recalcitrance mechanisms by organic matter molecular and chemical stabilization are overlapping stability which result from aggregates occlusion. As is difficult to form aggregates due to sandy soil matrix, carbon youth which enter the system is decomposed by microorganisms going to a more advanced stage of decomposition, forming in this case associations with silt and clay particles. This occurs in soils where the clay percentage is low. Through the fractionation it was observed that most of the soil organic matter is stored in the form of HU.

Objetivou-se com este estudo avaliar o carbono orgânico total e as frações físicas e químicas da matéria orgânica (MO) do solo em um Argissolo Vermelho Eutrófico arênico, submetido a diferentes sistemas de uso. Os sistemas de uso do solo avaliados foram: florestamento homogêneo de Eucalyptus grandis (EUC), sistema agrossilvipastoril na faixa (ASP) e campo nativo (CN), nas camadas de 0,000-0,025m e de 0,025-0,075m. A fração grosseira (CFG) e o carbono associado aos minerais (CAM) foram obtidos por meio de fracionamento físico granulométrico. As frações leve livre (FLL), leve oclusa (FLO) e pesada (FP) foram obtidas por meio de fracionamento físico densimétrico. As frações não húmicas (NH), ácido fúlvico (AF), ácido húmico (AH) e humina (HU) foram obtidas por meio de fracionamento químico. O sistema EUC promoveu maiores estoques de carbono orgânico total, CFG, FLL e FLO e na fração AF nas camadas avaliadas. Na camada superficial, os mecanismos de proteção da MO por recalcitrância molecular e estabilização química estão sobrepondo a estabilidade decorrente da oclusão em agregados. Com a dificuldade de formar agregados, devido a matrizes arenosas desse solo, o carbono jovem que entra no sistema é decomposto pelos microrganismos, entrando em um estágio mais avançado de decomposição e, nesse caso, formando associações com as partículas silte e argila, mesmo em solos em que o percentual de argila é baixo. Através do fracionamento químico, observou-se que a maior parte da MO do solo encontra-se armazenada na forma de HU.

Fracionamento químico e físico da matéria orgânica de um argissolo vermelho sob diferentes sistemas de uso

The objective of this study was to evaluate the total organic carbon and the physical and chemical fractions of organic matter in an Alfisol under different use systems. The land use systems evaluated was homogeneous forestry of Eucalyptus grandis, agrosilvopastoral system and native grassland, in layers from 0.000-0.025 and 0.025-0.075m depth. The coarse fraction (CFG) and the carbon associated minerals (CAM) were obtained by physical fractionation. The free light fraction (FLL), light occluded (FLO) and heavy (FP) were obtained by densimetric physical fractionation. The non-humicfractions (NH), fulvic acid (AF), humic acid (HA) and humin (HU) were obtained by chemical fractionation. The Eucalyptus grandis system promoted higher total organic carbon, CFG, FLL and FLO and AF. In the surface layer, the protective recalcitrance mechanisms by organic matter molecular and chemical stabilization are overlapping stability which result from aggregates occlusion. As is difficult to form aggregates due to sandy soil matrix, carbon youth which enter the system is decomposed by microorganisms going to a more advanced stage of decomposition, forming in this case associations with silt and clay particles. This occurs in soils where the clay percentage is low. Through the fractionation it was observed that most of the soil organic matter is stored in the form of HU.

Objetivou-se com este estudo avaliar o carbono orgânico total e as frações físicas e químicas da matéria orgânica (MO) do solo em um Argissolo Vermelho Eutrófico arênico, submetido a diferentes sistemas de uso. Os sistemas de uso do solo avaliados foram: florestamento homogêneo de Eucalyptus grandis (EUC), sistema agrossilvipastoril na faixa (ASP) e campo nativo (CN), nas camadas de 0,000-0,025m e de 0,025-0,075m. A fração grosseira (CFG) e o carbono associado aos minerais (CAM) foram obtidos por meio de fracionamento físico granulométrico. As frações leve livre (FLL), leve oclusa (FLO) e pesada (FP) foram obtidas por meio de fracionamento físico densimétrico. As frações não húmicas (NH), ácido fúlvico (AF), ácido húmico (AH) e humina (HU) foram obtidas por meio de fracionamento químico. O sistema EUC promoveu maiores estoques de carbono orgânico total, CFG, FLL e FLO e na fração AF nas camadas avaliadas. Na camada superficial, os mecanismos de proteção da MO por recalcitrância molecular e estabilização química estão sobrepondo a estabilidade decorrente da oclusão em agregados. Com a dificuldade de formar agregados, devido a matrizes arenosas desse solo, o carbono jovem que entra no sistema é decomposto pelos microrganismos, entrando em um estágio mais avançado de decomposição e, nesse caso, formando associações com as partículas silte e argila, mesmo em solos em que o percentual de argila é baixo. Através do fracionamento químico, observou-se que a maior parte da MO do solo encontra-se armazenada na forma de HU.

Kinetics of the aerobic decomposition of Talauma ovata and Saccharum officinarum / Cinéticas da decomposição aeróbia de Talauma ovata e Saccharum officinarum

The aim of this study is to evaluate the kinetics of aerobic decomposition of Saccharum officinarum and Talauma ovata leaves. For each species, decomposition chambers (leaves and water) were set up, which were maintained under controlled conditions. Each sampling day (1, 7, 15, 30, 39, 58, 72 and 90 days), the concentrations of total organic carbon, pH and electrical conductivity (EC) were determined in the dissolved fraction, while the mass and cell wall fractions (CWF) were determined in the particulate fraction. The pH stabilization of the chambers with T. ovata and S. officinarum leaves occurred in alkaline (ca. 8 - 8.5) and close to the neutrality (ca. 7 - 7.5) environment, respectively. The EC values were on average 1.6 times higher in incubations with T. ovata leaves. The mass loss did not differ between the species (mean = 53.85%), however the decay coefficient was higher for S. officinarum (k4 = 0.007 day-1) than for T. ovata (k4 = 0.005 day-1) leaves. The CWF mass loss (mean = 50.16%) and their coefficient (0.0090 day-1) were similar. S. officinarum decomposed faster due to its high concentrations of energetic compounds of interest to the microbiota. The slower decomposition of T. ovata may have occurred due to the presence of secondary compounds with negative effects to the microorganisms.

O objetivo deste estudo foi avaliar as cinéticas da decomposição aeróbia de folhas de Talauma ovata e Saccharum officinarum. Para cada espécie foram montadas câmaras de decomposição (folhas e água) que foram mantidas sob condições controladas. A cada dia amostral (1, 7, 15, 30, 39, 58, 72 e 90 dias), as concentrações de carbono orgânico total, pH e condutividade elétrica (CE) foram determinadas na fração dissolvida, enquanto a massa e as frações de parede celular (FPC) foram determinadas na fração particulada. A estabilização do pH das câmaras com folhas de T. ovata e S. officinarum ocorreram em meio básico (ca. 8 - 8,5) e próximo à neutralidade (ca. 7 - 7,5), respectivamente. Os valores de CE foram em média 1,6 vezes maiores nas incubações com folhas de T. ovata. A perda de massa não diferiu entre as espécies (média = 53,85%). No entanto, o coeficiente de decaimento foi maior para as folhas de S. officinarum (k4 = 0,007 dia-1) que para T. ovata (k4 = 0,005 dia-1). As perdas de massa da FPC (média = 50,16%) e seus respectivos coeficientes (0,0090 dia-1) foram similares. S. officinarum decompôs mais rapidamente devido às elevadas concentrações de compostos energéticos de interesse para a microbiota. A decomposição mais lenta de T. ovata pode ter ocorrido pela presença de compostos secundários com efeitos negativos sobre os micro-organismos.

Kinetics of the aerobic decomposition of Talauma ovata and Saccharum officinarum - doi: 10.4025/actascibiolsci.v34i4.9396

The aim of this study is to evaluate the kinetics of aerobic decomposition of Saccharum officinarum and Talauma ovata leaves. For each species, decomposition chambers (leaves and water) were set up, which were maintained under controlled conditions. Each sampling day (1, 7, 15, 30, 39, 58, 72 and 90 days), the concentrations of total organic carbon, pH and electrical conductivity (EC) were determined in the dissolved fraction, while the mass and cell wall fractions (CWF) were determined in the particulate fraction. The pH stabilization of the chambers with T. ovata and S. officinarum leaves occurred in alkaline (ca. 8 - 8.5) and close to the neutrality (ca. 7 - 7.5) environment, respectively. The EC values were on average 1.6 times higher in incubations with T. ovata leaves. The mass loss did not differ between the species (mean = 53.85%), however the decay coefficient was higher for S. officinarum (k4 = 0.007 day-1) than for T. ovata (k4 = 0.005 day-1) leaves. The CWF mass loss (mean = 50.16%) and their coefficient (0.0090 day-1) were similar. S. officinarum decomposed faster due to its high concentrations of energetic compounds of interest to the microbiota. The slower decomposition of T. ovata may have occurred due to the presence of secondary compounds with negative effects to the microorganisms.