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Abstract The aim of this study was to investigate the DGAT1 gene polymorphism and its effects on lamb weight in kazakh and tajik sheep breeds. A total of 97 blood samples were collected from purebred (еdilbay х еdilbay) and crossbred lambs (еdilbay x gissar) breеd by the Baiserke Agro Scientific and Production Center in the Talgar District of the Almaty Region of Kazakhstan. Animals were genotyped for DGAT1-AluI polymorphism using the polymerase chain reaction-restriction length polymorphism (PCR-RFLP) method. The result of PCR-RFLP showed that purebred (еdilbay х еdilbay) sheep had three genotypes (CC, CT and TT) and crossbred sheep had two genotypes (CC and CT). The predominant genotype was CC with a frequency of 0.70 and 0.58 in purebred sheep and crossbred sheep breeds, respectively. The DGAT1 gene showed no significant association with live weight of lambs at different times in both breeds studied. However, the study showed that the CC genotype produced higher live weight at day 60 in purebred sheep (CC: 33,668 kg and CT: 32,444) and at day 120 (CC: 41,487 and CT: 40,929) in crossbred lambs. The present study was the first to investigate the polymorphism and relationships between genotypes and lamb live weights for DGAT1 gene in sheep breeds, purebred and crossbred. We conclude that further comprehensive investigations should be done for the exact evidence of the effects of DGAT1/Aluı polymorphism on lamb live weights.

Resumo O objetivo deste estudo foi investigar o polimorfismo do gene DGAT1 e seus efeitos no peso do cordeiro em duas raças de ovelhas cazaques e tadjiques. Um total de 97 amostras de sangue foram coletadas de cordeiros de raça pura (еdilbay × еdilbay) e mestiços (еdilbay × gissar) criados pelo Baiserke Agro Scientific and Production Center no distrito de Talgar da região de Almaty do Cazaquistão. Os animais foram genotipados para o polimorfismo DGAT1-AluI usando o método de reação em cadeia da polimerase-polimorfismo de comprimento de restrição (PCR-RFLP). O resultado da PCR-RFLP mostrou que ovelhas de raça pura (еdilbay × еdilbay) tinham três genótipos (CC, CT e TT) e ovelhas mestiças tinham dois genótipos (CC e CT). O genótipo predominante foi CC com uma frequência de 0,70 e 0,58 em ovelhas de raça pura e mestiças, respectivamente. O gene DGAT1 não mostrou associação significativa com o peso vivo dos cordeiros, em diferentes momentos, em ambas as raças estudadas. No entanto, o estudo mostrou que o genótipo CC produziu maior peso vivo no dia 60 em ovelhas de raça pura (CC: 33,668 kg e CT: 32,444 kg) e, no dia 120, (CC: 41,487 kg e CT: 40,929 kg) em cordeiros mestiços. O presente estudo foi o primeiro a investigar o polimorfismo e as relações entre genótipos e pesos vivos de cordeiros para o gene DGAT1 em raças de ovelhas, puras e mestiças. Concluímos que investigações mais abrangentes devem ser feitas para a evidência exata dos efeitos do polimorfismo DGAT1/AluI nos pesos vivos dos cordeiros.

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The objective of this study was to evaluate the yield, morphometric traits, and the primal and sub primal cuts of Santa Inês lamb carcasses and their crossbreds with Dorper rams finished in feedlot. Thirty uncastrated lambs of the following genetic groups were used: 10 Santa Inês, 10 ½Dorper + ½Santa Inês and 10 -Dorper + »Santa Inês at 115 days of age averaging 20kg of live body weight. The experiment period was 53 days, preceded by seven days for adaptation. The carcasses of the Dorper crossbred lambs had higher carcass weight and yield, as well as larger thorax and rump width, and larger thorax, rump, and leg perimeter. The carcasses of the Dorper crossbred lambs also showed greater weight for all primal and sub primal carcass cuts, except for the neck. The lamb carcasses of Santa Inês had higher yields of shoulder and neck primal cuts and the carcasses of Dorper crossbred lambs showed higher loin yield. The carcasses of the crossbred lambs had higher yield of sirloin, rump steak, and frenched rack cap. The genetic group influences the linear and circular measures of the lamb carcasses and the weight of primal and sub primal cuts.

Objetivou-se avaliar o rendimento, as características morfométricas e os cortes primários e secundários da carcaça de cordeiros Santa Inês e de suas cruzas com reprodutores Dorper terminados em confinamento. Trinta cordeiros foram utilizados dos seguintes grupos: 10 Santa Inês, 10 ½ Dorper + ½ Santa Inês e 10 - Dorper + » Santa Inês, que apresentaram, no início do experimento, 115 dias de idade média e 20kg de peso vivo médio. O período experimental foi de 53 dias, precedidos de sete dias para adaptação. As carcaças dos cordeiros mestiços Dorper tiveram maiores pesos e rendimentos, bem como maiores larguras do tórax e da garupa e maiores perímetros do tórax, da garupa e da perna. As carcaças dos cordeiros mestiços Dorper também apresentaram maiores pesos para os cortes primários e secundários da carcaça, exceto para o corte do pescoço. As carcaças dos cordeiros Santa Inês apresentaram maiores rendimentos de cortes primários de paleta e pescoço, e as carcaças dos cordeiros mestiços apresentaram maior rendimento do lombo. As carcaças dos cordeiros mestiços obtiveram maiores rendimentos de picanha, lombo e carré francês. O grupo genético influencia medidas lineares e circulares da carcaça de cordeiros e o peso dos cortes primários e secundários.

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This study evaluated heterosis and reciprocal effects in across of White Leghorn with Fayoumi, White Leghorn with Koekoek in comparison to their pure line breeds for the productive and reproductive traits. Data were purposively collected from 357 layers based on their genetic groups and each genotype was reared in three replicates on a deep litter system from 20-72 weeks. Results showed that genotype groups were found to have no significant differences (p > 0.05) for age at first egg (AFE) and hen- housed egg production (HHEP). Purebred Koekoek and crossbred of male White Leghorn and female Koekoek were superior for body weight at first egg (BWAFE). Purebred Fayoumi has exhibited the least values for egg number. Purebred White Leghorn was the best by their feed conversion ratio. Effects of heterosis were non-significant (p > 0.05) for AFE and BWAFE, and hen-housed egg production. A negative heterosis percentage was noted for AFE in all crossbred genotypes. Crossing between male Koekoek and female White Leghorn gave the highest heterosis effect for feed intake while crossing between male White Leghorn and female Fayoumi gave the highest estimates of heterosis for total egg number per hen. The main crossbred between male White Leghorn and female Fayoumi hybrids outperformed in AFE and HHEP while the main crossbred between male White Leghorn and female Koekoek outperformed in BWAFE and HHEP. On the contrary, reciprocal crossbreds between male Fayoumi and female White Leghorn and male Koekoek and female White Leghorn had higher feed intake and better feed conversion ratio.(AU)

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We evaluated the reproductive losses in three periods: period I ­ starting from the time the cows were exposed to the bulls to pregnancy diagnoses (PD, number of cows diagnosed as non-pregnant/total exposed cows × 100); period II ­ from the time of PD to calving (number of calving cows/number of cows diagnosed as pregnant × 100); and period III ­ from calving to weaning (number of weaned calves/number of calving cows × 100) in purebred Hereford (HH) and Angus (AA) cows and their crosses in beef cows under extensive production systems. Likewise, the effect of parity (nulliparous, primiparous, and multiparous) and the interaction between both factors were studied. A thirteen-year data set (2505 record) of an experimental breeding herd, maintained in an extensive production system based on natural grassland, was used. The dataset was under a complete diallel design between HH and AA breeds. Both the genetic group and parity of the cow affected the reproductive losses, but only in period I. No interaction was found. Purebred cows had higher reproductive losses than the crossbred cows, without differences between the purebred (HH and AA) or between the crossbred (AH and HA). The greatest losses were observed rather in primiparous than in nulliparous and multiparous cows without difference between the latter two. The use of crossbred cows in extensive production systems is an alternative to reduce reproductive losses and to increase calf harvest.(AU)

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Supplementation strategies have been studied to increase animal performance and the quality of meat. Thus, this study was carried out to evaluate the influence of different supplementation strategies on the performance, feed intake, digestibility and ingestive behaviour of crossbred steers on Brachiaria brizantha pasture, during the dry season. The experiment was performed in Bahia, Brazil, from August 2015 to January 2016, with a dry season of 203 days. Thirty-three crossbred steers with an average weight of 269.5 ± 41.8 kg were distributed in a completely randomised design with three treatments and 11 replicates per treatment. The results were analysed using an ANOVA and a Tukey test, with a 5% probability of error. The treatments were mineral salt with urea and the supplementation of protein based on the animals' body weight (BW), as follows: 0.1 and 0.2% BW. There was no effect of supplementation strategy on the initial and final body weights. Supplementation strategies did not influence (p > 0.05) the variables related to the intake of total dry matter. There was an effect (p < 0.05) on the dry matter and crude protein digestibility coefficients. For ingestive behaviour, feed intake, grazing time and idle time tended to be altered by the supply strategies (p < 0.05), but rumination was not affected (p > 0.05) by the evaluated strategies. In conclusion, the supplementation strategies used during the dry season resulted in a similar performance; therefore, the adopted strategy can be chosen according to the economic and regional conditions of the production system. Thus, the strategy adopted could be chosen according to the economic and regional conditions of the production.(AU)

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The objective of this work was to evaluate the non-carcass components of crossbred Santa Inês lambs finished in feedlot with the inclusion of different levels of red propolis extract (RPE). Thirty-five crossbred lambswere used with an average weight of 17.08 ± 2.36 kg and mean age of five months, distributed in a completely randomized design with five treatments and seven replications. The treatments evaluated were: 0; 7; 14 and 21 mL of extract/animal/dayBefore slaughter, each animal was individually weighed to obtain the body weight at slaughter (BWS). The weights and yields of non-carcass components were evaluated, weights and yields of regional dishes (Buchadaand Panelada). The addition of different levels of red propolis extract (RPE) did not influence (p >0.05) the weights and yields of non-carcass components, by-products and adipose deposits, to the weight and yield of regional dishes. The use of red propolis extract in diets up to the level studied (28 mLday-1) did not change the characteristics evaluated.(AU)

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The article presents the results of scientific research on the production of lamb in the conditions of the Akmola region. The experiment was conducted on purebred Kazakh short-tailed rough-haired and cross-bred sheep obtained from industrial crossing of Kazakh short-tailed rough-haired queens with sheep- producers of the hampshire breed. Further, the article presents the results of feeding and fattening, as well as the morphological composition of the carcass of sheep of the Kazakh coarse-haired sheep breed of different ages. The object of the study for feeding and fattening were three groups of experimental sheep of the above-mentioned breed, where compound feed was included in the diet of the I experimental group in the form of top dressing, in the II experimental group, grain waste was also used for top dressing, and the control group was kept in the feed without top dressing. Based on the study of the feeding of experimental sheep, it was found that the absolute increase during the feeding period in experimental sheep who received fertilization in the form of compound feed is higher than in their peers, respectively, by 0.2 and 1.49 kg. In terms of morphological and varietal composition, in particular, the number of cuts of grade 1, there was also an advantage of experimental sheep that received fertilization in the form of compound feed compared to their peers, respectively, by 2.3-8.3%. In general, the results of a study on the growth, development and slaughter qualities of experimental sheep, i.e. purebred Kazakh short-tailed rough-haired and crossbred, obtained from crossing with sheep producers of the precocious breed "hampshire" showed that from the moment of birth to 2 and 4 months. For example, crossbred sheep were slightly superior to purebred peers in terms of the studied indicators. In particular, the results of feeding sheep of the Kazakh coarse-haired broad-tailed breed from 4 to 6 months, depending on the top dressing.

Este artigo apresenta os resultados de pesquisas científicas sobre a ovinocultura nas condições da região de Aqmola. O experimento foi conduzido em ovinos cazaques raça pura de cauda curta e lã grossa, em comparação com ovinos mestiços obtidos a partir do cruzamento industrial de fêmeas cazaques de cauda curta e lã grossa com ovinos da raça Hampshire. Ademais, o presente artigo apresenta os resultados da alimentação e engorda, bem como a composição morfológica da carcaça de ovelhas cazaques da raça de lã grossa de diferentes idades. O objeto deste estudo relativo à alimentação e engorda se resumiu a três grupos experimentais de ovinos da raça supramencionada, onde alimentos compostos foram incluídos na dieta do Grupo Experimental I sob a forma de cobertura, enquanto no Grupo Experimental II foram também utilizados resíduos de cereais para a forragem e o Grupo de Controle foi mantido em um regime de alimentação sem cobertura. Com base no estudo da alimentação de ovinos experimentais, verificou-se que o crescimento absoluto durante o período de alimentação em ovinos experimentais que receberam fertilização sob a forma de alimentos compostos é superior aos seus pares em 0,2 e 1,49 kg, respectivamente. Em termos de composição morfológica e varietal, particularmente quanto ao número de cortes superiores, observou-se também uma vantagem de ovinos experimentais que receberam fertilização sob a forma de alimentos compostos em comparação com seus pares em 2,3-8,3%, respectivamente. Em geral, os resultados do estudo sobre as características de crescimento, desenvolvimento e abate de ovinos experimentais, ou seja, tanto da raça pura cazaque de cauda curta e lã grossa quanto da raça mestiça obtida a partir do cruzamento com produtores de ovinos precoces da raça Hampshire, obtiveram estes resultados desde o momento do nascimento até o segundo e quarto mês de vida. Por exemplo, os resultados dos ovinos mestiços foram ligeiramente superiores quando comparados aos seus pares de raça pura em relação aos indicadores aqui estudados. Específicamente a respeito dos resultados da alimentação de ovelhas cazaques de lã grossa e cauda larga de 4 a 6 meses, de acordo com a cobertura utilizada.

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This research aimed to compare statistical methods (random forest, RIDGE, LASSO, and elastic net regression) for the prediction of body weight in purebred and crossbred pigs reared in Brazil. This prediction was based on dorsal-view images obtained from video image processing. The study involved 69 animals belonging to breeds such as Large White, Piau, Duroc × Large White, and Piau × Large White. The data collection spanned 144 days, with measurements taken at approximately 20-day intervals, totaling eight measurements for each animal throughout their growth stages. Image acquisition was carried out in individual pens using an Intel RealSense Depth D435 digital camera. The features back area, back perimeter, back width, and body depth were extracted from the images. Pearson's correlation analysis was conducted to assess the relationship between live weight and these features. The dataset was randomly divided into a training dataset (65%) and a test dataset (35%), and model training was performed by five-fold cross-validation balanced according to the growth stage, which was divided into three groups. This procedure was repeated 100 times, and the resulting metrics were taken as the average of the 100 repetitions. Although with a slight difference, the random forest method outperformed the others with the highest average R2 value (0.87), as well as the lowest average RMSE (14.32) and average MAE (10.13) values. Consequently, the random forest algorithm proved to be the most effective in predicting body weight. The back area, back width, and back perimeter were the most important variables in the model.(AU)

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This study evaluated the productive and reproductive performances of Santa Inês sheep and their Dorper crossbreeds during the lactation period amidst the dry season in the semi-arid region of Paraíba, Brazil. Conducted at the experimental goat farm of the State University of Paraíba (UEPB), Campus IV, Catolé do Rocha, the investigation included a cohort of 24 ewes (12 Santa Inês and 12 Dorper crossbreeds [comprising 87.5% Dorper and 12.5% Santa Inês]) and 30 lambs (10 males and 20 females). Observations were recorded from birth and continued at 30-day intervals up to 90 days. Using a two-way ANOVA with a 2 x 2 factorial design (two genetic groups and two sexes), the study monitored both ewes and lambs. The animals adhered to traditional regional management, grazing in paddocks by day and secured in shelters by dusk. The study found that both Santa Inês and Dorper crossbred ewes produced similar quantities of milk (P=0.8310) for the first 60 days of lactation. There was no discernible effect of the ewes' genetic backgrounds on their live weights, body condition scores at lambing, throughout the subsequent 30, 60, and 90 days of lactation, or on the overall productive efficiency and total weight of weaned lambs per ewe. In contrast, Dorper lambs demonstrated a higher weaning weight (P=0.0349) and greater weight gain (P=0.0403). The study also noted that the sex of the lambs did not notably influence their performance within the first 90 days of suckling.

Objetivou-se avaliar o desempenho produtivo e reprodutivo de ovinos da raça Santa Inês e mestiços da raça Dorper criados em sistema tradicional de produção durante a fase de amamentação na época seca do ano no semiárido paraibano. O experimento foi conduzido no Setor de Caprinovinocultura da Universidade Estadual da Paraíba/UEPB/Campus-IV, Catolé do Rocha/PB. Foram utilizadas 24 ovelhas multíparas, sendo 12 da raça Santa Inês e 12 mestiças da raça Dorper (87,5% Dorper + 12,5% Santa Inês) paridas com 30 ovinos jovens, sendo 10 machos e 20 fêmeas. O período experimental se iniciou no momento do parto e os dados foram coletados ao nascimento, aos 30, 60 e 90 dias de idade. Foi utilizada análise de variância para as ovelhas e para os cordeiros esquema fatorial 2 x 2 (dois grupos genéticos e dois sexos). Os cordeiros permaneceram com suas mães em piquetes durante o dia e à noite no aprisco, manejo tradicional da região. As ovelhas Santa Inês e mestiças Dorper produziram quantidades de leite semelhantes (P=0,8310) até os 60 dias de lactação. O grupo genético das ovelhas não influenciou os pesos vivos e escore de corporal ao parto, 30, 60 e 90 dias de lactação, como também a eficiência produtiva e peso total de crias desmamadas por ovelha. Os cordeiros e cordeiras Dorper apresentaram maiores pesos vivos (P=0,0349) e ganhos de peso (P=0,0403), por outro lado, o sexo dos ovinos jovens não influenciou o desempenho até os 90 dias de amamentação.

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The use of crop residue silages is a viable alternative for producers to reduce feed costs. The present study was developed with the objective of investigating the inclusion of pineapple silage in replacement of sorghum silage in the diet of lactating cows regarding feed intake, production, and quality of milk. Eight crossbred (Holstein × Gir) cows with an average weight of 555±30 kg and an average milk yield of 12.50±3.25 kg/day, between 60 and 90 days in milk, were distributed into two simultaneous 4 × 4 Latin squares, at one animal per experimental unit. Silage of pineapple crop residue (PS) using plants after the harvest of the fruits was included in the roughage portion of the diet at increasing levels, with simultaneous and proportional removal of sorghum silage (SS) from the diets. The studied treatments thus consisted of the roughage portion containing: 1- 0% PS and 100% SS (100SS); 2- 34% PS and 66% SS (34PS66SS); 3- 67% PS and 33% SS (67PS33SS); 4- 100% PS and 0% SS (100PS). The diets were supplied twice daily, allowing 5% leftovers in relation to the quantity offered. The amounts of feed supplied, and orts of each animal were weighed for a subsequent determination of intake. The experiment was divided into four 21-day sub-periods, the first 14 days of which were used as an adaptation to the diet, while the others served for data collection. The milk was weighed from the 15th to the 19th day of each experimental period, and milk samples were collected on the last day of each period. Milk samples were sent to the laboratory, where they were analyzed. Dry matter, mineral matter, and lignin concentrations in the diet did not vary (P>0.05) as a function of PS inclusion in the roughage portion, while protein content, fiber content, and fat content levels were influenced. No effect of PS inclusion was observed (P>0.05) on the intakes of dry matter, neutral detergent fiber, mineral material, or lignin. However, PS inclusion prompted (P<0.05) a linear increase in the intakes of protein and total fat. The inclusion of pineapple plant silage in the roughage part of the diet had a positive effect (P<0.05) on milk yield, 4% fat-corrected milk yield, and on the milk fat and total solids contents. Milk production, as well as fat and total solids content, increased moderately with inclusion of pineapple plant silages in the roughage portion of the diet. Therefore, pineapple silage can replace sorghum silage in the roughage part of diets for lactating crossbred cows.(AU)

A utilização de silagens de resíduos culturais é uma alternativa viável para os produtores reduzirem custos com alimentação. O presente estudo foi desenvolvido com o objetivo de investigar a inclusão da silagem de abacaxi em substituição à silagem de sorgo na dieta de vacas lactantes quanto ao consumo de ração, produção e qualidade do leite. Oito vacas mestiças (Holstein × Gir), com peso médio de 555±30 kg e produção média de leite de 12,50±3,25 kg/dia, entre 60 e 90 dias de lactação, foram distribuídas em dois quadrados latinos 4 × 4 simultâneos, em um animal por unidade experimental. A silagem de resíduo da cultura do abacaxi (PS) utilizando plantas após a colheita dos frutos foi incluída na porção de volumoso da dieta em níveis crescentes, com retirada simultânea e proporcional da silagem de sorgo (SS) das dietas. Os tratamentos estudados consistiram, portanto, na porção de volumoso contendo: 1- 0% PS e 100% SS (100SS); 2- 34% PS e 66% SS (34PS66SS); 3- 67% PS e 33% SS (67PS33SS); 4- 100% PS e 0% SS (100PS). As dietas foram fornecidas duas vezes ao dia, permitindo sobras de 5% em relação à quantidade oferecida. As quantidades de ração fornecida e sobras de cada animal foram pesadas para posterior determinação do consumo. O experimento foi dividido em quatro subperíodos de 21 dias, sendo os primeiros 14 dias utilizados como adaptação à dieta, enquanto os demais serviram para coleta de dados. O leite foi pesado do 15º ao 19º dia de cada período experimental, e as amostras de leite foram coletadas no último dia de coleta de cada período. As amostras de leite foram enviadas ao laboratório, onde foram analisadas. As concentrações de matéria seca, matéria mineral e lignina na dieta não variaram (P>0,05) em função da inclusão de PS na porção de volumoso, enquanto os teores de proteína, teor de fibra e teor de gordura foram influenciados. Não foi observado efeito da inclusão de PS (P>0,05) nos consumos de matéria seca, fibra em detergente neutro, material mineral ou lignina. No entanto, a inclusão de PS provocou (P<0,05) um aumento linear na ingestão de proteínas e gorduras totais. A inclusão de silagem de abacaxi na parte volumosa da dieta teve efeito positivo (P<0,05) na produção de leite, na produção de leite corrigida em 4% para gordura e nos teores de gordura e sólidos totais do leite. A produção de leite, bem como o teor de gordura e sólidos totais aumentaram moderadamente com a inclusão de silagens de abacaxi na porção de volumoso da dieta. Portanto, a silagem de abacaxi pode substituir a silagem de sorgo na parte de volumoso das dietas de vacas mestiças em lactação.(AU)

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This study aimed to evaluate food additives for the replacement of monensinand virginiamycin in high-grain diets for cattle. A Latin square experimental design was adopted, represented by four treatments (functional oil, yeast, tannin and control monensin and virginiamycin) in four experimental periods, with four animals cannulated in the rumen. l The natural ruminal modulators fully replaced the ionophores monensin and virginiamycin in the concentrate. Being evaluated: ruminal pH, apparent digestibility and in situdegradability of dry matter. The results showed that the pH variation curve as a function of rumen fluid collection time was not significant (P<0.05) among the additives studied. The additives used to replace monensin and virginiamycin in crossbred cattle fed a diet of 70% concentrate and 30% roughage with fractionation five times a day, are efficient in maintaining rumen pH above 6.0. The additives did not affect digestibility of OM, CP, NDF, FDA and EE.The in situ degradability of the soluble and potentially degradable fractions was higher in the yeast and essential oil treatments, respectively.The additives evaluated are efficient in replacing monensin and virginiamycin.

Objetivou-se com o presente estudo avaliar aditivos alimentares em substituição a monensina mais virginiamicina em dietas de alto grão para bovinos. Foi adotado o delineamento experimental em quadrado latino, representado por quatro tratamentos (Óleo funcional, Levedura, Tanino e o controle monensina e virginiamicina) em quatro períodos experimentais, com quatro animais cânulados no rúmen. Os moduladores ruminais naturais substituíram integralmente os ionóforos monensina mais virginiamicina no concentrado. Sendo avaliados: pH ruminal, digestibilidade aparente e a degradabilidade in situda matéria seca. Os resultados mostraram que a curva de variação do pH em função do tempo de coleta de líquido ruminal não foi significativa (P<0,05) entre os aditivos estudados. Os aditivos utilizados em substituição a monensina mais virginiamicina para bovinos mestiços com dieta 70% concentrado e 30% volumoso com fracionamento cinco vezes ao dia, são eficientes na manutenção do pH ruminal acima de 6,0. Não diferindo os aditivos na digestibilidade aparente de MO, PB, FDN, FDA e EE. A degradabilidade in situ apresentou maiores valores para a fração solúvel e potencialmente degradável nos tratamentos com levedura e óleos essenciais, respectivamente. Sendo os aditivos avaliados eficientes na substituição a monensina e virginiamicina.

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The impact of GnRH treatment on the day of TAI in beef cows has received limited investigation, especially concerning its association with estrus expression. Consequently, two experiments were conducted to assess the potential of GnRH treatment on the day of TAI to enhance fertility according to the expression or not of estrus in beef cows. Experiment 1 aimed to determine ovulation rate and luteal function, while Experiment 2 aimed to determine the effect of the two GnRH treatment approaches on pregnancy rate. In Experiment 1, multiparous Brangus suckling cows (n = 17) were submitted to an 8-day TAI protocol. Estrus occurrence was evaluated based on chalk removal on D10 (TAI) and cows were assigned to receive GnRH (25µg lecirelin; im) according to the group: GnRH (n = 7), regardless of estrus expression; or selectGnRH (n = 10), only cows not detected in estrus. Ovulation rate occurring until 77h after IVD removal did not differ (p = 0.17) between GnRH (85.7%; 6/7) and selectGnRH (100%; 10/10). Also, corpus luteum size and serum progesterone concentration were not affected (p>0.05) by treatments. In Experiment 2, crossbred taurine suckled cows (n = 384) were submitted to the same protocol as described in Experiment 1 and were randomly allocated to GnRH or selectGnRH groups. There was no difference in P/AI between groups (selectGnRH = 55.6%; GnRH = 54.3%; p = 0.7) 30 days after TAI. As expected, there was a pronounced effect (p<0.0001) of estrus expression on P/AI (Estrus = 61.5%; No estrus = 33.0%), regardless of group. In summary, ovulation timing and rate and luteal function did not differ between groups. Also, GnRH administration only in cows that do not show estrus is recommended, considering hormone savings and similar conception rate.(AU)

Resumo

As malformações congênitas surgem no desenvolvimento embrionário e podem ser funcionais ou estruturais, afetando diversos órgãos e tecidos, podendo ter como causas fatores ambientais, genéticos ou iatrogênicos. Dentre essas malformações, pode-se citar a microtia, que se caracteriza pela malformação da pina, resultando em uma hipoplasia de pavilhão auricular externo, apresentando anormalidade no tamanho, contorno ou ausência total da aurícula e lóbulo, condição que na maioria dos casos é unilateral e resulta em perda auditiva, especialmente quando associada à atresia do canal auditivo. O objetivo deste trabalho foi relatar os principais achados clínicos em um caso de microtia e atresia do conduto auricular externo, congênito em um bovino no estado do Amazonas-BR. Foi atendido pelo Serviço Médico Veterinário do Instituto Federal de Educação, Ciência e Tecnologia do Amazonas - IFAM, campus Lábrea, uma bezerra mestiça (Jersey + Girolando), para manejo neonatal. Durante o exame físico do animal foi observada alteração anatômica na pina esquerda, caracterizada por microtia de pina e ausência do conduto auditivo externo. Não foram observadas alterações anatômicas na orelha direita. Este caso destaca a necessidade de exame físico e avaliação clínica minuciosa em neonatos, pois anomalias auditivas podem não apresentar sinais neurológicos evidentes.(AU)

Congenital malformations arise during embryonic development and can be functional or structural, affecting various organs and tissues, and may be caused by environmental, genetic, or iatrogenic factors. Among these malformations, microtia can be mentioned, which is characterized by malformation of the pinna, resulting in hypoplasia of the external ear, which presents as an abnormality in the size, contour, or total absence of the auricle and lobe, a condition that in most cases is unilateral and often results in hearing loss, especially when associated with atresia of the auditory canal. The objective of this study was to report the main clinical findings in a case of microtia and atresia of the external ear canal, congenital in a bovine in the state of Amazonas. A crossbred (Jersey + Girolando) heifer was treated by the Veterinary Medical Service of the Federal Institute of Education, Science, and Technology of Amazonas - IFAM, Lábrea campus, for neonatal management. During the physical examination of the animal, an anatomical alteration was observed in the left pinna, characterized by pinna microtia and the absence of the external auditory canal. No anatomical alterations were observed in the right ear. This case highlights the need for thorough physical examination and clinical evaluation in neonates, as hearing anomalies may not present with obvious neurological signs.(AU)

Las malformaciones congénitas surgen en el desarrollo embrionario y pueden ser funcionales o estructurales, afectando diversos órganos y tejidos, y pueden ser causadas por factores ambientales, genéticos o iatrogénicos. Entre estas malformaciones podemos mencionar la microtia, que se caracteriza por la malformación del pabellón auricular, dando lugar a una hipoplasia del pabellón auricular externo, presentando una anomalía en el tamaño, contorno o ausencia total del pabellón auricular y lóbulo de la oreja, condición que en la mayoría En algunos casos es unilateral y produce pérdida de audición, especialmente cuando se asocia con atresia del canal auditivo. El objetivo de este trabajo fue relatar los principales hallazgos clínicos en un caso de microtia y atresia del conducto auditivo externo, congénita en un bovino en el estado de Amazonas-BR. Un ternero mestizo (Jersey + Girolando) fue atendido por el Servicio Médico Veterinario del Instituto Federal de Educación, Ciencia y Tecnología de Amazonas - IFAM, campus Lábrea, para manejo neonatal. Durante el examen físico del animal se observaron cambios anatómicos en el pabellón auricular izquierdo, caracterizados por microtia del pabellón auricular y ausencia del conducto auditivo externo. No se observaron cambios anatómicos en el oído derecho. Este caso resalta la necesidad de un examen físico y una evaluación clínica exhaustiva en los recién nacidos, ya que las anomalías auditivas pueden no presentar signos neurológicos obvios.(AU)

Resumo

The present study presents a comparative evaluation of the transit kinetics of straw briquette in response to the dietary addition of non-protein nitrogen sources in the form of a mineral supplement. Four rumen-cannulated, castrated Holstein-Gir crossbred cattle, weighing an average of 380±22.64 kg, were distributed into a 4 × 4 Latin square design (four supplements with non-protein nitrogen sources × four experimental periods). The following non-protein nitrogen sources were studied: conventional urea, slow-release urea, extruded urea, and monoammonium phosphate. During the experiment, the animals were housed in individual stalls with concrete floors where they received a basal diet consisting of straw briquette, potato starch, and the mineral supplement, the latter whose variation was only in the non-protein nitrogen source, which characterized the treatments. The different non-protein nitrogen sources did not affect the parameters of transit or degradation kinetics of straw fiber briquette. These results can be associated with the low nitrogen content limited by the types of supplements and the particle size of straw briquette, which is smaller due to processing.(AU)

Resumo

The aim of this study was to evaluate the effects of four crude glycerin levels in the diet of feedlot lambs on performance, carcass characteristics, and meat quality. Forty three-month-old crossbred Santa Inês x Dorper lambs weighing 18.2 ± 0.169 kg were used in a completely randomized design, with four diets and 10 replicates per diet. The animals were housed in individual pens. The trial period (42 days) was preceded by 14 days of adaptation to handling and diet. Final body weight (FBW), empty body weight (EBW), average daily weight gain, feed conversion, and feed efficiency did not differ with crude glycerin levels in the diet. Nutrient intake (dry matter (DM), ether extract (EE), crude protein (CP), and neutral detergent fiber (NDF)) did not differ depending on crude glycerin levels. In the evaluation of carcass characteristics, there was a quadratic effect due to crude glycerin levels on hot carcass yield (maximum point- maxP = 9.73%), leg perimeter (maxP = 9.45%), fat thickness (maxP = 7.41%), and leg weight (maxP = 8.69%). Loin weight and conformation showed a linear increase as a function of crude glycerin levels. The other studied variables were not affected by crude glycerin levels in the diet. In the non-carcass components, the full gastrointestinal tract, mesenteric fat, omental, and perirenal fat were not influenced by crude glycerin levels. Regarding meat quality, no effects were observed on the parameters evaluated in the longissimus dorsi. The addition of up to 15% crude glycerin in the lamb's diet does not affect its performance, however, the addition of up to 8% improves carcass characteristics and maintains meat quality.(AU)

O objetivo deste estudo foi avaliar os efeitos de quatro níveis de glicerina bruta na dieta de cordeiros confinados sobre o desempenho, características de carcaça e qualidade da carne. Foram utilizados 40 cordeiros mestiços Dorper x Santa Inês com três meses de idade e peso corporal médio de 18,2 ± 0,169 kg, distribuídos em delineamento inteiramente casualizado, com quatro dietas e 10 repetições por dieta. Os animais foram alojados em baias individuais. O período experimental (42 dias) foi precedido de 14 dias de adaptação ao manejo e às dietas. O peso corporal final, peso de corpo vazio, ganho de peso médio diário, conversão alimentar e eficiência alimentar não diferiram com os níveis de glicerina bruta na dieta. O consumo de nutrientes (matéria seca, extrato etéreo, proteína bruta e fibra em detergente neutro) não diferiu em função dos teores de glicerina bruta. Na avaliação das características de carcaça, houve efeito quadrático devido aos níveis de glicerina bruta sobre o rendimento de carcaça quente (ponto máximo-maxP = 9,73%), perímetro da perna (maxP = 9,45%), espessura de gordura (maxP = 7,41%) e o peso da perna (maxP = 8,69%). O peso do lombo e a conformação apresentaram aumento linear em função dos teores de glicerina bruta. As demais variáveis estudadas não foram afetadas pelos níveis de glicerina bruta na dieta. Nos componentes não carcaça, verificou-se que o sistema gastrointestinal completo, gordura mesentérica, omental e gordura perirrenal não foram influenciados pelos níveis de glicerina bruta. Na qualidade da carne, não foi observado efeito nos parâmetros avaliados no longissimus dorsi. A inclusão de até 15% de glicerina bruta na dieta do cordeiro não afeta seu desempenho, porém a inclusão de até 8% melhora as características da carcaça e mantém a qualidade da carne.(AU)

Resumo

The objective of this study was to examine the effects of various ventilation modalities in sheep anesthetized with isoflurane or propofol. Twelve healthy adult crossbred ewes, aged 12 months and weighing 32±5 kg, were used in the study. After instrumentation with conscious animals in the right lateral decubitus position, they were administered morphine (0.3 mg kg-1) and detomidine (20 mcg kg-1) as preanesthetic medication. General anesthesia was induced with propofol (4 mg kg-1), followed by intubation. The sheep were then allocated into two groups: PG (n=6), which received continuous infusion of propofol (0.5 mg kg-1 min-1) for maintenance; and IG (n=6), which was maintained with isoflurane inhalation anesthesia (1.3 V %). Each group underwent four different ventilation modalities for 30 min: spontaneous ventilation (SV), volume-cycled mechanical ventilation (VV), pressure-cycled mechanical ventilation (PV), and pressure-cycled mechanical ventilation with positive end-expiratory pressure (PPV). Heart rate and cardiac index were higher in the IG, while systemic vascular resistance was higher in the PG. Hemoglobin levels, intrapulmonary shunt fraction, and tidal volume were higher in the PG. Both groups developed metabolic alkalosis. Isoflurane anesthesia resulted in greater hypotension, reduced lung compliance, increased airway resistance, lower PaO2, and higher PaCO2 values across all ventilation modalities. In contrast, PPV led to improved lung compliance, oxygenation index, and higher PaO2 values, with lower intrapulmonary shunt fractions. The study concluded that propofol and PPV provide greater hemodynamic stability in anesthetized sheep.(AU)

O objetivo deste estudo foi avaliar diferentes modalidades ventilatórias em ovinos anestesiados com isoflurano ou propofol. Foram utilizadas 12 ovelhas, adultas, mestiças, com 12 meses de idade, pesando 32±5 kg, comprovadamente hígidas. Após instrumentação com os animais conscientes em decúbito lateral direito, foi administrado morfina (0,3 mg kg-1) e detomidina (20 mcg kg-1) como MPA. Foram induzidas à anestesia geral com propofol (4 mg kg-1) e intubadas. Neste momento, foram alocadas em dois grupos: PG (n=6), manutenção com infusão contínua de propofol (0,5 mg kg min-1), e IG (n=6) com anestesia inalatória com isoflurano (1,3 V%). Todas foram mantidas por 30 minutos em cada modalidade ventilatória: ventilação espontânea (VE), ventilação mecânica ciclada a volume (VV), ventilação mecânica ciclada a pressão (VP) e ventilação mecânica ciclada a pressão com PEEP (VPP). A FC e o IC foram maiores em IG e já o IRVS foi maior em PG. Os valores de hemoglobina, fração de shunt intrapulmonar e o volume corrente foram maiores no PG. Os animais apresentaram alcalose metabólica em ambos os grupos. O isoflurano promoveu maior hipotensão, diminuição da complacência pulmonar, aumento da resistência das vias aéreas, menores valores de PaO2 e maiores valores de PaCO2 em todas as modalidades ventilatórias. A VPP promoveu maior complacência pulmonar e índice de oxigenação, maiores valores de PaO2 e menores valores de shunt intrapulmonar. Conclui-se que o propofol e a VPP promoveram maior estabilidade hemodinâmica em ovelhas.(AU)

Resumo

In reproductive technologies, uncovering the molecular aspects of oocyte and embryo competence under different conditions is crucial for refining protocols and enhancing efficiency. RNA-seq generates high-throughput data and provides transcriptomes that can undergo additional computational analyses. This study presented the transcriptomic profiles of in vitro matured oocytes and blastocysts produced in vitro from buffalo crossbred (Bubalus bubalis), coupled with gene co-expression and module preservation analysis. Cumulus Oophorus Complexes, obtained from slaughterhouse-derived ovaries, were subjected to in vitro maturation to yield metaphase II oocytes (616) or followed in vitro fertilization and culture to yield blastocysts for sequencing (526). Oocyte maturation (72%, ±3.34 sd) and embryo development (21.3%, ±4.18 sd) rates were obtained from three in vitro embryo production routines following standard protocols. Sequencing of 410 metaphase II oocytes and 70 hatched blastocysts (grade 1 and 2) identified a total of 13,976 genes, with 62% being ubiquitously expressed (8,649). Among them, the differentially expressed genes (4,153) and the strongly variable genes with the higher expression (fold-change above 11) were highlighted in oocytes (BMP15, UCHL1, WEE1, NLRPs, KPNA7, ZP2, and ZP4) and blastocysts (APOA1, KRT18, ANXA2, S100A14, SLC34A2, PRSS8 and ANXA2) as representative indicators of molecular quality. Additionally, genes exclusively found in oocytes (224) and blastocysts (2,200) with specific biological functions were identified. Gene co-expression network and module preservation analysis revealed strong preservation of functional modules related to exosome components, steroid metabolism, cell proliferation, and morphogenesis. However, cell cycle and amino acid transport modules exhibited weak preservation, which may reflect differences in embryo development kinetics and the activation of cell signaling pathways between buffalo and bovine. This comprehensive transcriptomic profile serves as a valuable resource for assessing the molecular quality of buffalo oocytes and embryos in future in vitro embryo production assays.(AU)

Resumo

This trial was designed to evaluate the effect of processing method of rehydrated corn grain silage (RCGS) on feed intake, performance, carcass traits, feeding behavior, rumen morphology, and blood metabolites of cattle in the finishing phase. Thirty-eight F1 Angus × Nellore bulls (365±22 kg) raised under grazing conditions were housed in individual pens (2 × 4 m) for the feeding trial. At the end of the adaptation period, animals were weighed after a 16-h fast, blocked by shrunk body weight, and randomly assigned to one of two dietary treatments: GRC, consisting of RCGS that was ground before ensiling using a hammer mill with a uniform 8-mm screen (1.52 mm geometric mean particle size); and RRC, consisting of RCGS that was rolled before ensiling in a roller mill mounted in a bagging machine (2.18 mm geometric mean particle size). Diet ingredients were mixed manually twice daily, at 09:00 and 15:00 h, and offered as total mixed rations in amounts approximately 50 g/kg in excess of daily intake. The experimental diets contained a forage:concentrate ratio of 130:870 g/kg, with 644 g/kg of RCGS. The GRC resulted in greater daily variation in dry matter intake, total-tract digestibility (dry and organic matter), fecal pH, rumen papillae width, and lower first meal duration, meal length, fecal starch, and rumen papillae height than the RRC. However, grinding or rolling RCGS did not affect dry matter intake, growth performance, carcass traits, or health (liver abscesses, ruminitis, serum D-lactate) of finishing beef cattle. Therefore, the processing equipment to make RCGS might be preferable based on equipment availability, milling yield, energy consumption, and diet composition.(AU)

Resumo

The objective of this study was to assess the effects of high-concentrate diets with and without the inclusion of soybean hull (SH; 0 or 500.8 g/kg) plus corn (whole or ground) on the performance, morphometric traits, and nutritional and metabolic parameters of crossbred dairy calves. We also aimed to assess the costs associated with the experimental diets employed. Thirty-six male calves with an average weight of 89±18.6 kg and age of 3.5 months were allocated to four treatments, with nine replicates of one calf/pen, in a completely randomized experimental design for 198 days. The inclusion of the SH and the physical form of corn did not influence animal performance or morphometric measurements; however, SH inclusion increased the digestibility of neutral detergent fiber and the intakes of dry matter and neutral detergent fiber, but did not affect total digestible nutrient intake. The physical form of corn did not influence nutrient intake or digestibility, which resulted in similar performance across the animals. Blood glucose levels as well as alkaline phosphatase and creatinine remained elevated regardless of the diet. The use of SH reduced the total plasma cholesterol content, and there was an interaction between the physical form of corn and SH inclusion in relation to creatinine levels, which were higher when using diets with whole corn and SH. Although the inclusion of up to 500.8 g/kg of SH in the diet reduces feed efficiency, its use decreases the cost per kilogram of weight gain. Dairy calves can exhibit satisfactory production performance when fed alternative ingredients such as whole corn or SH, which have a lower cost than ground corn, traditionally used in diet formulations.(AU)

Resumo

This study aimed to investigate the impact of pig breed on stress levels and meat quality in two commercial pig farms in the north-eastern part of Romania. One farm raised 52 Mangalita pure-bred pigs and the other 82 Duroc x Landrace crossbred pigs. Serum cortisol, a biomarker of stress, and serum glucose were measured before and after slaughtering, while meat pH was measured at 45 minutes and 24 hours post-mortem. Results showed that the Duroc x Landrace crossbred pigs had higher serum cortisol and glucose values before and after slaughtering compared to the Mangalita purebred pigs. The meat pH values of the two breeds were similar, with the pH45 and pH24 values being 6.67 and 5.48 for Duroc x Landrace crossbred and 6.53 and 5.52 for Mangalita purebred, respectively. These results suggest that pig breed can have an impact on stress levels and meat quality, with Duroc x Landrace crossbred pigs showing higher stress levels and meat quality being not affected by the breed of pig. Further research is needed to confirm these findings and to understand the underlying mechanisms.

Este estudo teve como objetivo investigar o impacto da raça suína nos níveis de estresse e na qualidade da carne em duas fazendas comerciais de suínos, no nordeste da Romênia. Uma fazenda criou 52 suínos puros Mangalita, e a outra 82 suínos mestiços Duroc x Landrace. O cortisol sérico, um biomarcador de estresse e a glicose sérica foram medidos antes e após o abate, enquanto o pH da carne foi medido aos 45 minutos e 24 horas post mortem. Os resultados mostraram que os suínos mestiços Duroc x Landrace apresentaram valores séricos de cortisol e de glicose mais elevados, antes e após o abate, em comparação com os suínos puros Mangalita. Os valores de pH da carne das duas raças foram semelhantes, sendo os valores de pH45 e pH24 de 6,67 e 5,48 para o mestiço Duroc x Landrace e de 6,53 e 5,52 para o puro sangue Mangalita, respectivamente. Esses resultados sugerem que a raça suína pode ter um impacto nos níveis de estresse e na qualidade da carne, com os porcos mestiços Duroc x Landrace apresentando níveis mais altos de estresse e a qualidade da carne não sendo afetada pela raça do porco. Mais pesquisas são necessárias para confirmar esses achados e entender os mecanismos subjacentes.