Fibroblastic osteosarcoma in a guinea pig (Cavia porcellus)

The article describes the case of a 3 year 6 months old Guinea Pig (Cavia porcellus) that was assisted at the Wildlife and Exotic Animal Practice of the Faculty of Veterinary Sciences, National University of Asunción. The patient presented a hard mass in the right hind limb extending from the proximal portion of the femur, projecting caudally to the proximal tuberosity of the calcaneus, with an increase in temperature, and a marked increase in sensitivity. Radiographic diagnosis refers to a mass with amorphous areas of mineral density. Fine needle aspiration puncture revealed an undifferentiated malignant proliferative process, requiring histopathological analysis to characterize the process. Disarticulation was performed and subsequent histopathologic diagnosis under hematoxylin-eosin and Masson's trichrome stain revealed fibroblastic osteosarcoma.(AU)

Extraskeletal osteosarcoma in the duodenum of a dog

Background: Osteosarcoma is the most observed primary bone tumor in dogs, and may affect the appendicular and axial skeletons. In addition, it may be present in extraskeletal form, accounting for only 1% of cases. As shown by few reports in the literature, the involvement of the intestinal region by is rare. The objective of this study was to report the case of a 13-year-old Yorkshire dog, submitted to an exploratory laparotomy for suspected partial intestinal obstruction, diagnosed with extraskeletal osteosarcoma. Case: A 13-year-old dog, Yorkshire Terrier, male, presented clinical signs of gastrointestinal abnormalities. An ultrasound examination was performed and was found a mass in small intestine region with wall and lumen invasion. Then, was realized exploratory laparotomy and detected intestinal obstruction due to a mass with approximately 5.0 x 6.0 x 4.4 cm localized in duodenum. Surgical removal was performed and the sample sent to the veterinary diagnostic laboratory for histopathological examination. The sample had an irregular surface and firm consistency. In addition, when cut, the mass enveloped the intestinal layers and sometimes obstructed the lumen. Then, the sample were processed routinely for histopathology. After that, in microscopy evaluation was detected cell proliferation, affecting all layers of intestine. In detail, cells were elongated with pleomorphism marked and atypical mitosis. In addition, there was production of cartilage and bone matrix. So, due the absence of others sites, the neoplasm was considered primary of intestine. After that, to evaluate the expression of KI-67 and COX-2 was performed, and the cell proliferation index was 54.0% and the COX-2 expression was moderate in less than 10% of neoplastic cells. After the surgery, the patient was hospitalized for a week and continue the treatment in home. Afterwards, the tutor received the diagnosis, but even though he was instructed on the severity of the case, he chose not to undergo chemotherapy. After three months, the patient presented abdominal fluid and nodules in your liver, suggesting metastasis, but without diagnosis confirmation. The patient died five months after the diagnosis of extraskeletal osteosarcoma. However, no necropsy was realized, impossibility the diagnosis confirm. Discussion: The frequency of extraskeletal osteosarcoma in dogs remains unknown, with the mammary glands being the most affected site. In the present study, osteosarcoma affects the duodenal region and no reports of this neoplasm in the duodenum of dogs have been found in the literature. The clinical sign of dyschezia was important for the tutor to refer the animal to the veterinarian and perform the ultrasound in an attempt to elucidate the case, as the tumor mass is not always palpable. Histopathological examination and immunohistochemistry were necessary for the differential diagnosis and to establish the prognosis, although after the surgery the tutor chose not to perform chemotherapy. Extraskeletal osteosarcoma are usually highly metastatic, mainly affecting the lymph nodes and liver. In this case, the patient presented a liver nodule three months after the tumor removal surgery, but unfortunately, there was no diagnostic confirmation. Such neoplastic type is rarer and more aggressive than appendicular and axial osteosarcoma, with an average survival of 1 to 3 months. In this case, as a necropsy was not obtained, we cannot attribute the survival time to the disease. The survival rates of osteosarcomas in dogs are few months, but in the present case, although the patient died five months after surgery, the failure to perform a necropsy compromises the attribution of survival time to extraskeletal osteosarcoma.

A comparative analysis of anatomopathological features and COX-2 expression of mammary neoplasms with malignant mesenchymal components in female dogs / Análise comparativa das características anatomopatológicas e expressão de COX2 em neoplasias mamárias com componentes mesenquimais malignos em cadelas

ABSTRACT: Canine mammary neoplasms with malignant mesenchymal components, such as carcinosarcomas and sarcomas, belong to an uncommon and histologically heterogeneous group. Little is known about the biological behavior of these histogenic variants. This study aimed to compare the clinicopathological characteristics and the COX-2 immunohistochemical expression of different histologic subtypes of carcinosarcomas and sarcomas. Samples of 23 carcinosarcomas and 15 sarcomas from the mammary glands of female dogs were studied. Medical records were reviewed to obtain clinical data. Subsequently, histology microscope slides were analyzed to assess for mesenchymal subtypes, necrosis, vascular invasion, histologic grades, and lymph node metastasis. Immunohistochemistry was used to assess the COX-2 expression. The malignant mesenchymal proliferation was categorized into osteosarcomas (23/40), fibrosarcomas (5/40), liposarcomas (6/40) and chondrosarcomas (4/40). The osteosarcomatous differentiation was the most predominant type among the sarcomas and carcinosarcomas and was associated with vascular invasion (P=0.010) and lymph node metastases (P=0.014). High COX-2 expression was detected in 14.3% of the carcinosarcomas (carcinoma and/or sarcoma cells) and 27.3% of the sarcomas. The carcinosarcomas and sarcomas had similar clinical and pathological characteristics and developed as large tumors, with intratumoral necrosis and a predominance of high histologic grades, although the frequency of vascular invasion and lymph node metastasis was low. Osteosarcoma subtypes presented more aggressive characteristics than non-osteosarcoma subtypes.

RESUMO: Neoplasias mamárias caninas com componentes mesenquimais malignos, como carcinossarcomas e sarcomas, são um grupo de neoplasias pouco frequentes e histologicamente heterogêneas e pouco se sabe sobre o comportamento biológico das variantes histogênicas. O objetivo desse estudo é comparar as características anatomopatológicas e a expressão imunoistoquímica de COX-2 de diferentes subtipos histológicos de carcinossarcomas e sarcomas. Foram estudados 23 carcinosarcomas e 17 sarcomas da glândula mamária de cadelas. Os prontuários médicos foram revisados para obtenção de dados clínicos. Posteriormente, as lâminas histológicas foram avaliadas para acessar os subtipos mesenquimais, necrose, invasão vascular, grau histológico, metástase linfonodal. A imunoistoquímica foi realizada para avaliar a expressão de COX-2. Os tipos encontrados de proliferação mesenquimal maligna foram osteossarcoma (23/40), fibrossarcoma (7/40), lipossarcoma (6/40) e condrossarcoma (4/40). A diferenciação osteossarcomatosa foi predominante entre os sarcomas e carcinossarcomas e foi associado com invasão vascular (P=0,006) e metástase linfonodal (P=0,014). Uma expressão alta de COX-2 foi detectada em 14,3% dos carcinossarcomas (células carcinomatosas e/ou sarcomatosas) e 27,3% dos sarcomas. Os carcinossarcomas e sarcomas apresentaram características clínicas e patológicas semelhantes e se desenvolveram como tumores grandes, com necrose intratumoral e predomínio de alto grau histológico, mas com baixa frequência de invasão vascular e metástase distante. Os subtipos osteossarcomatosos apresentaram características mais agressivas quando comparados com subtipos não osteossarcomatosos.

Recycled pequi (Caryocar brasiliense, Camb) shell ethanolic extract induces apoptosis in canine osteosarcoma cell / Extrato etanólico de cascas recicladas de pequi (Caryocar brasiliense, Camb) induz apoptose em células de osteossarcoma canino

Osteosarcoma is a mesenchymal cancer associated with high mortality in dogs and in humans. The biodiversity-rich Cerrado, the predominant biome in the Midwest region of Brazil, is native to locally appreciated fruits such as pequi (Caryocar brasiliense, Camb). Although this plant has been frequently used in folk medicine, the pharmacological properties of pequi fruit shells have not been fully evaluated. Thus, this study aimed to determine the cytotoxic activity of ethanol extract of recycled pequi shells on canine osteosarcoma cells in vitro. Cells were cultured and treated with final extract concentrations of 0, 0.029 µg/µL, 0.29 µg/µL, and 2.91 µg/µL for 24, 48, or 72 hours. Cell viability assay using trypan blue exclusion method and tetrazolium reduction method, cell survival assay, and double labeling with annexin V and propidium iodide were performed in the treated osteosarcoma cells. These allowed the determination of IC50, survival fraction, and type of cell death, respectively. Pequi shell ethanol extract at a concentration of 2.91 µg/µL showed the greatest inhibition of osteosarcoma cell growth in vitro, resulting in a 71.80% decrease in growth compared to the control. The mean IC50 was 155.2 µg/mL at 72 hours. The calculated survival fractions showed that cell growth at 72 hours was 3.33% lower in cells treated with 2.91 µg/µL extract. Results from the double labeling experiment suggest that apoptosis was the predominant type of cell death in cells treated with 2.91 µg/µL extract. These results demonstrate that ethanol extract of recycled pequi shells promotes apoptosis in canine osteosarcoma cells.

O osteossarcoma é uma neoplasia mesenquimal associada à alta mortalidade em cães e humanos. O pequi (Caryocar brasiliense, Camb.) é nativo do Cerrado, bioma rico em biodiversidade predominante na região Centro-Oeste do Brasil. Embora esta planta seja frequentemente utilizada na medicina popular, as propriedades farmacológicas da casca do pequi ainda são pouco conhecidas. Este estudo teve como objetivo determinar a atividade citotóxica do extrato etanólico de cascas de pequi recicladas em células de osteossarcoma canino. As células foram cultivadas e tratadas com concentrações finais de extrato de zero, 0,029 µg/µL, 0,29 µg/µL e 2,91 µg/µL por 24, 48 ou 72 horas. As células foram submetidas a ensaio de viabilidade celular pelos métodos de exclusão de azul de tripano da redução de tetrazólio para determinar a IC50, ao ensaio de sobrevivência celular para calcular a fração de sobrevivência e à dupla marcação com anexina V e iodeto de propídio para determinar o tipo de morte celular. O extrato etanólico de casca de pequi na concentração de 2,91 µg/µL apresentou a maior inibição do crescimento de células de osteossarcoma, com redução de 71,80% no crescimento em relação ao controle. A IC50 média foi de 155,2 µg/mL em 72 horas. O crescimento celular em 72 horas foi 3,33% menor nas células tratadas com 2,91 µg/µL de extrato. A apoptose foi o tipo predominante de morte celular em células tratadas com 2,91 µg/µL de extrato. Esses resultados demonstram que o extrato etanólico de cascas de pequi recicladas promove apoptose em células de osteossarcoma canino.

Utilização da manufatura aditiva (impressão em 3d) no tratamento de osteossarcoma vertebral em cão submetido a vertebrectomia lombar: relato de caso

El osteosarcoma es una de las neoplasias óseas más comúnmente reportadas en medicina veterinaria, principalmente en perros de raza grande y gigante, siendo que la forma vertebral tiene importante escenario clínico presentando el 5% del total de reportes. Tiene un pronóstico desfavorable y un tiempo de supervivencia corto. La presentación clínica es variable y pueden observarse desde signos de dolor intenso hasta déficits neurológicos como resultado de la afectación medular progresiva. El diagnóstico puede realizarse mediante imágenes con radiografía o tomografía computarizada, y mediante análisis de laboratorio de citopatología o histopatología, siendo este último preferible para la clasificación del tumor. La resección parcial del tumor es una forma de tratamiento paliativo, indicada en algunos casos para aliviar temporalmente los signos clínicos relacionados con el dolor y los déficits neurológicos. Sin embargo, cuando hay posibilidad terapéutica, la escisión completa proporciona un mejor pronóstico y tiempo de supervivencia, principalmente cuando se asocia con quimioterapia o radioterapia. Mediante el procedimiento de la vertebrectomía, es necesaria la estabilización quirúrgica, que puede realizarse respetando los principios para la estabilización vertebral por traumatismo, siendo necesaria la colocación de un espaciador vertebral para evitar el colapso medular. Se han propu

Osteosarcoma is one of the most commonly reported bone neoplasms in veterinary medicine, especially in large and giant breed dogs, and the vertebral form has an important clinical scenario presenting 5% of the total reports. This has an unfavorable prognosis and a short survival time.  The clinical presentation is variable and signs of severe pain to neurological deficits can be observed as a result of progressive spinal cord involvement. The diagnosis can be made by imaging with radiography or computed tomography, and by laboratory analysis of cytopathology or histopathology, the latter being preferable for tumor classification. Partial tumor resection is a palliative form of treatment, indicated in some cases to temporarily relieve clinical signs related to pain and neurological deficits. However, when there is therapeutic possibility, complete excision provides better prognosis and survival time, especially when associated with chemotherapy or radiotherapy. Through the vertebrectomy procedure, surgical stabilization is necessary and can be performed following the principles for vertebral stabilization due to trauma, with the placement of a vertebral spacer being necessary to prevent spinal collapse. Several materials have been proposed for the manufacture of the spinal spacer. Additive manufacturing, also known as 3D printing, has transformed the concept of rapid pr

O osteossarcoma é uma das neoplasias ósseas mais comumente relatadas na medicina veterinária, principalmente em cães de raça grande e gigante, sendo que a forma vertebral possui importante cenário clínico apresentando 5% do total de relatos. Este possui um prognóstico desfavorável e tempo de sobrevida pequeno. A apresentação clínica é variável e podem ser observados sinais de dor intensa a déficits neurológicos em decorrência do envolvimento medular progressivo. O diagnóstico pode ser realizado por imagens através de radiografia ou tomografia computadorizada, e por análises laboratoriais de citopatologia ou histopatologia, sendo o último preferível para classificação de tumores. A ressecção tumoral parcial é uma forma paliativa de tratamento, indicada em alguns casos para aliviar temporariamente os sinais clínicos relacionados a dor e déficits neurológicos. Contudo, quando há possibilidade terapêutica, a excisão completa fornece melhor prognóstico e tempo de sobrevida, principalmente quando associada a quimioterapia ou radioterapia. Mediante procedimento de vertebrectomia, a estabilização cirúrgica é necessária, podendo ser realizada respeitando os princípios para estabilização vertebral por trauma, sendo necessário a colocação de um espaçador vertebral para evitar o colabamento medular. Diversos materiais foram propostos para a confecção do espaçador vertebral. A manufatura ad

Utilização da manufatura aditiva (impressão em 3D) no tratamento de osteossarcoma vertebral em cão submetido a vertebrectomia lombar: relato de caso / Use of additive manufacturing (3D printing) in the treatment of vertebral osteosarcoma in dogs submitted to lumbar vertebrectomy: case report / Uso de la fabricación aditiva (impresión 3D) en el tratamiento del osteosarcoma vertebral en perros sometidos a vertebrectomía lumbar: caso clínico

O osteossarcoma é uma das neoplasias ósseas mais comumente relatadas na medicina veterinária, principalmente em cães de raça grande e gigante, sendo que a forma vertebral possui importante cenário clínico apresentando 5% do total de relatos. Este possui um prognóstico desfavorável e tempo de sobrevida pequeno. A apresentação clínica é variável e podem ser observados sinais de dor intensa a déficits neurológicos em decorrência do envolvimento medular progressivo. O diagnóstico pode ser realizado por imagens através de radiografia ou tomografia computadorizada, e por análises laboratoriais de citopatologia ou histopatologia, sendo o último preferível para classificação de tumores. A ressecção tumoral parcial é uma forma paliativa de tratamento, indicada em alguns casos para aliviar temporariamente os sinais clínicos relacionados a dor e déficits neurológicos. Contudo, quando há possibilidade terapêutica, a excisão completa fornece melhor prognóstico e tempo de sobrevida, principalmente quando associada a quimioterapia ou radioterapia. Mediante procedimento de vertebrectomia, a estabilização cirúrgica é necessária, podendo ser realizada respeitando os princípios para estabilização vertebral por trauma, sendo necessária a colocação de um espaçador vertebral para evitar o colabamento medular. Diversos materiais foram propostos para a confecção do espaçador vertebral. A manufatura aditiva, também conhecida como impressão 3D, tem transformado o conceito de prototipagem rápida em realidade, devido a habilidade de fabricar peças geométricas específicas de alta complexidade e de forma rápida, permitindo elaborar protótipos para uso pré ou transoperatórios em cirurgias ortopédicas de alta complexidade. O polietileno tereftalato glicol (PETG), por ser um termoplástico de alta resistência, não-biodegradável, biocompatível e de baixo custo é foco desse trabalho no desenvolvimento do espaçador vertebral como estabilizador em um procedimento de vertebrectomia em cão.

Osteosarcoma is one of the most commonly reported bone neoplasms in veterinary medicine, especially in large and giant breed dogs, and the vertebral form has an important clinical scenario presenting 5% of the total reports. This has an unfavorable prognosis and a short survival time. The clinical presentation is variable and signs of severe pain to neurological deficits can be observed as a result of progressive spinal cord involvement. The diagnosis can be made by imaging with radiography or computed tomography, and by laboratory analysis of cytopathology or histopathology, the latter being preferable for tumor classification. Partial tumor resection is a palliative form of treatment, indicated in some cases to temporarily relieve clinical signs related to pain and neurological deficits. However, when there is therapeutic possibility, complete excision provides better prognosis and survival time, especially when associated with chemotherapy or radiotherapy. Through the vertebrectomy procedure, surgical stabilization is necessary and can be performed following the principles for vertebral stabilization due to trauma, with the placement of a vertebral spacer being necessary to prevent spinal collapse. Several materials have been proposed for the manufacture of the spinal spacer. Additive manufacturing, also known as 3D printing, has transformed the concept of rapid prototyping into reality, due to its ability to quickly manufacture specific geometric parts of high complexity, allowing the elaboration of prototypes for pre- or trans-operative use in highly complex orthopedic surgeries. Polyethylene terephthalate glycol (PETG), being a high-strength, non-biodegradable, biocompatible and low-cost thermoplastic, is the focus of this work in the development of the vertebral spacer as a stabilizer in a vertebrectomy procedure in a dog.

El osteosarcoma es una de las neoplasias óseas más comúnmente reportadas en medicina veterinaria, principalmente en perros de raza grande y gigante, siendo que la forma vertebral tiene importante escenario clínico presentando el 5% del total de reportes. Tiene un pronóstico desfavorable y un tiempo de supervivencia corto. La presentación clínica es variable y pueden observarse desde signos de dolor intenso hasta déficits neurológicos como resultado de la afectación medular progresiva. El diagnóstico puede realizarse mediante imágenes con radiografía o tomografía computarizada, y mediante análisis de laboratorio de citopatología o histopatología, siendo este último preferible para la clasificación del tumor. La resección parcial del tumor es una forma de tratamiento paliativo, indicada en algunos casos para aliviar temporalmente los signos clínicos relacionados con el dolor y los déficits neurológicos. Sin embargo, cuando hay posibilidad terapéutica, la escisión completa proporciona un mejor pronóstico y tiempo de supervivencia, principalmente cuando se asocia con quimioterapia o radioterapia. Mediante el procedimiento de la vertebrectomía, es necesaria la estabilización quirúrgica, que puede realizarse respetando los principios para la estabilización vertebral por traumatismo, siendo necesaria la colocación de un espaciador vertebral para evitar el colapso medular. Se han propuesto varios materiales para la fabricación del espaciador vertebral. La fabricación aditiva, también conocida como impresión 3D, ha transformado el concepto de prototipado rápido en una realidad, debido a la capacidad de fabricar piezas geométricas específicas de gran complejidad y con rapidez, lo que permite la elaboración de prototipos para su uso pre o trans-operatorio en cirugías ortopédicas de gran complejidad. El polietileno tereftalato glicol (PETG), por ser un termoplástico de alta resistencia, no biodegradable, biocompatible y de bajo costo es el foco de este trabajo en el desarrollo del espaciador vertebral como estabilizador en un procedimiento de vertebrectomía en un perro.

Turmeric ethanol extract (Curcuma longa L. ) reduces apoptosis and promotes canine osteosarcoma cell proliferation / Extrato etanólico de açafrão (Curcuma longa L. ) reduz apoptose e promove proliferação de células de osteossarcoma canino

Curcuma longa L., also known as turmeric, has been widely studied for its various therapeutic properties, including antineoplastic action. The ethanolic extract of the plant contains several phenolic compounds, especially curcumin. Osteosarcoma is a predominant bone tumor in dogs and humans, characterized by high metastatic potential and an unfavorable prognosis. The aim of this study was to investigate the effects of turmeric ethanol extract on canine osteosarcoma cells from established culture. The cells were cultured and treated with different curcumin concentrations (0, 10 µM, 20 µM, 50 µM, 100 µM, and 1000 µM) and exposure times (24h, 48h, and 72h). We first performed tetrazolium reduction technique (MTT) assay and calculated IC50. An immunocytochemistry assay was performed after extract treatment to verify the expression of mutated p53 and therefore study the proliferative potential of malignant cells; Bcl-2 and Ki-67 were used to assess apoptosis and the degree of malignancy, respectively. The extract enhanced the proliferation of canine osteosarcoma cells, reaching 3,819.74% at 50 µM of curcumin. The extract also significantly altered the expression of mutated p53 and Ki-67 proteins but not that of Bcl-2, suggesting that it did not induce this antiapoptotic pathway. Overall, these results are prerequisite to better understanding how natural compounds such as turmeric ethanolic extract affect cell proliferation and could be used to treat various diseases.

A Curcuma longa L., planta conhecida popularmente como açafrão, tem sido amplamente estudada por suas diversas propriedades terapêuticas, incluindo a ação antineoplásica. O extrato etanólico da planta contém diversos compostos fenólicos, com destaque para a curcumina. O osteossarcoma é um tumor ósseo predominante em cães e humanos, caracterizado por apresentar alto potencial metastático e prognóstico desfavorável. Procurou-se investigar os efeitos de diferentes concentrações de curcumina do extrato etanólico de açafrão sobre células de osteossarcoma canino de cultura estabelecida. As células foram cultivadas e submetidas ao tratamento com extrato com diferentes concentrações de curcumina (0, 10 µM, 20 µM, 50 µM, 100 µM e 1000 µM) e tempos de exposição (24h, 48h e 72h) pelo EEA. Inicialmente, foram realizados: técnica de redução do tetrazólio (MTT) e cálculo da IC50. Posteriormente, após o tratamento com o extrato, realizou-se o ensaio de imunocitoquímica para verificar a expressão de p53 mutada e estudar o potencial proliferativo das células malignas; Bcl-2, com intuito de averiguar o estímulo de via antiapoptótica; e o marcador Ki-67, que sinaliza aumento no grau de malignidade. O extrato promoveu proliferação de células de osteossarcoma canino, com incremento de até 3819,74% na concentração de 50µM de curcumina. O composto também alterou a expressão das proteínas p53 mutante e Ki-67 significativamente, mas não alterou a expressão de Bcl-2, mostrando que não induziu a via antiapoptótica mediada por esta. Estes resultados demonstram que o extrato etanólico do açafrão apresenta potencial proliferativo sobre células de osteossarcoma canino, sugerindo a necessidade de conscientização e conhecimento dos reais efeitos de determinados compostos naturais, considerados seguros ao serem utilizados como tratamento de diversas enfermidades.

Partial hemipelvectomy: an alternative for improving dogs' life quality / Hemipelvectomia parcial: alternativa para melhoria na qualidade de vida em cães

Partial hemipelvectomy refers to the surgical removal of a pelvic segment, which is performed for pelvic bone neoplasms, severe fractures, or poor bone union. This study presents the cases of seven dogs, six of which have neoplasms involving the hip joint and one with severe pelvic canal narrowing due to a poorly consolidated trauma. Partial caudal hemipelvectomy was performed for all the dogs to improve the quality of life and delay euthanasia. Of the seven cases, six required the use of a polypropylene mesh for the pelvic wall closure, with 85.71% of them acquiring manageable complications in the postoperative period. Despite these complications, an overall improvement in the quality of life was reported to be significant in the operated animals due to the removal of the cause of pain or discomfort. Thus, it was concluded that partial hemipelvectomyis beneficial for dogs with pelvic conditions. Furthermore, the main complication due to the lack of a pelvic wall for primary occlusion was circumvented by using a polypropylene mesh, showing that possible complications in this procedure can be appropriately managed.

A hemipelvectomia parcial consiste na remoção de segmento pélvico, indicada para neoplasmas com envolvimento de ossos da pelve, fraturas graves ou má união óssea. Esse trabalho apresenta sete pacientes caninos, dos quais seis portadores de neoplasmas envolvendo a articulação coxofemoral e um com grave estreitamento do canal pélvico, resultado de traumatismo mal consolidado. Com o intuito de fornecer qualidade de vida aos cães e postergar a eutanásia, todos foram operados pela técnica de hemipelvectomia parcial média a caudal. Dos sete pacientes, seis necessitaram do uso de tela de polipropileno para fechamento da parede pélvica e apresentaram 85,71% de complicações manejáveis no pós-operatório. No entanto, a melhora na qualidade de vida dos animais operados foi significativa, tendo sido removida a causa da dor ou desconforto desencadeado pela alteração anatômica local. Conclui-se que a técnica é benéfica aos pacientes, tendo como principal complicação, a falta de parede pélvica para oclusão primária, contornada com uso de tela de polipropileno pelos autores.

Hemangiossarcoma pouco diferenciado grau II em seio nasal esquerdo de cão: relato de caso / Grade II poorly differentiated hemangiosarcoma in the left nasal sinus of dog: case report

Hemangiossarcoma é um câncer com alto poder de malignidade e metástase, tendo origem em células endoteliais de vasos sanguíneos, é frequentemente encontrado em baço, pele e raramente em cavidade nasal de cães. Foi atendido um cão, SRD, de 13 anos, com secreção nasal hemorrágica em narina esquerda e aumento de volume dorsal ao nariz que se estendia até seio frontal, com exoftalmia de globo ocular esquerdo, tendo se instalado de forma aguda. Pela radiografia de crânio nas projeções latero-lateral obliqua e dorso ventral, observou-se lise óssea dos seios nasal, frontal e orbital. Colhido material para Citopatologia, o diagnóstico presuntivo foi osteossarcoma. A fim de se planejar o ato cirúrgico, realizou se a tomografia computadorizada de crânio, que evidenciou formação na cavidade nasal esquerda, com expansão até o osso orbital, sem acometimento de linfonodos regionais. A fim de se avaliar a hemostasia e possibilidades de tromboembolismo no pós-cirúrgico, realizou-se o tromboelastograma e o paciente tinha possibilidades de realizar trombo. Ele foi operado com sucesso, removida a maior parte da massa tumoral e utilizada a eletroquimioterapia no leito cirúrgico. O diagnóstico histopatológico foi hemangiossarcoma pouco diferenciado grau 2. O paciente recebeu tratamento com anti-coagulante nos dias seguintes, teve uma recuperação satisfatória e iniciou-se a quimioterapia adjuvante com doxorubicina após 1 mês da cirurgia, entretanto, ele apresentou quadro de sangramento difuso compatível com CID e veio a óbito. Os hemangiossarcomas são raros na cavidade nasal de cães e parece adotar um pior comportamento nesta região, quando comparado ao baço e pele, inclusive com possibilidade de síndrome para-neoplásica. O trombolestograma tem se mostrado um exame importante para avaliação do equilíbrio hemostático no controle as doenças neoplásicas.

Hemangiosarcoma is a cancer with high power of malignancy and metastasis, originating in endothelial cells of blood vessels, it is often found in the spleen, skin and rarely in the nasal cavity of dogs. A dog, SRD, 13 years old, was treated with hemorrhagic nasal discharge in the left nostril and increase in volume dorsal to the nose that extended to the frontal sinus, with exophthalmos of the left eyeball, which had been installed acutely. On the skull X-ray in the lateral oblique and dorsal-ventral projections, bone lysis of the nasal, frontal and orbital sinuses was observed. After collecting material for cytopathology, the presumptive diagnosis was osteosarcoma. In order to plan the surgical act, a computed tomography scan of the skull was performed, which showed formation in the left nasal cavity, with expansion up to the orbital bone, without involvement of regional lymph nodes. In order to assess hemostasis and possibilities of thromboembolism in the postoperative period, a thromboelastogram was performed and the patient had possibilities of having a thrombus. He was successfully operated on, removing most of the tumor mass and using electrochemotherapy in the surgical bed. The histopathological diagnosis was grade 2 poorly differentiated hemangiosarcoma. The patient received treatment with anticoagulants in the following days, had a satisfactory recovery and adjuvant chemotherapy with doxorubicin was started 1 month after surgery, however, he presented compatible diffuse bleeding. with ICD and died. Hemangiosarcomas are rare in the nasal cavity of dogs and seem to have a worse behavior in this region when compared to the spleen and skin, including the possibility of paraneoplastic syndrome. The thrombolestogram has been shown to be an important test for evaluating the hemostatic balance in the control of neoplastic diseases.

Meningeal osteosarcoma in a dog’s brain / Osteossarcoma meningeal no encéfalo de um cão

Osteosarcoma is the most common bone cancer in dogs. It has a high invasion capacity and commonly metastasizes. This neoplasia ensues mainly in the medullary canal of long bones of the appendicular and axial skeleton, rarely affecting primarily extra-skeletal sites. Primary meningeal osteosarcomas are extremely rare both in human and veterinary medicine. A male, mixed breed dog was admitted at the Veterinary Hospital with a history of over excitement, decreased neurological reflexes, and seizures. The worsening of its clinical condition led to its euthanasia and anatomopathological examination at the Veterinary Pathology Service. This report describes a primary extra-skeletal osteosarcoma of a dog’s meninges. Its clinical signs were indicative of a central nervous system disease later diagnosed by histopathological and immunohistochemical analysis.

O osteossarcoma é considerado a neoplasia óssea mais frequente em cães. Tem elevada capacidade invasiva e comumente gera metástase. Essa neoplasia é mais frequentemente observada no canal medular de ossos longos do esqueleto apendicular e axial. É raro que acometa primariamente sítios extraesqueléticos. O osteossarcoma primário meningeal é considerado extremamente raro em medicinas humana e veterinária. Um cão macho e sem raça definida foi atendido no Hospital Veterinário com histórico de excitação, diminuição dos reflexos neurológicos e desenvolvimento de quadro convulsivo. O agravamento do quadro clínico levou à eutanásia do animal e a um exame anatomopatológico pelo Serviço de Patologia Veterinária. O objetivo deste relato é descrever um caso de osteossarcoma extra-esquelético primário de meninge em um cão que apresentou quadro clínico compatível com doença originária do sistema nervoso central e que foi diagnosticado por meio das análises histopatológica e imunohistoquímica.

Osteosarcoma of the hard palate in a dog - case report / [Osteossarcoma em palato duro de cão - relato de caso]

The osteosarcoma (OSA) is the most diagnosed primary bone cancer in canine patients. This work reports a case of a canine, six years old, mongrel, female, intact, with an OSA in the hard palate. Physical examination detected a firm mass in the palate. Thoracic radiographs, hematological and biochemical exams, histopathological exams and computed tomography were requested. A chondroblastic OSA was diagnosed and the tumor was characterized by immunohistochemistry. There was never evidence of metastasis in this case. The treatment consisted of the combination of conventional chemotherapy, metronomic chemotherapy, and palliative care, aiming at greater survival and well-being of the patient since surgical excision was not possible due to the location and extension of the tumor. Osteogenic sarcomas of the hard palate are rarely seen and described in the literature. In this article we present a characterization of the osteosarcoma with uncommon localization in the hard palate.(AU)

O osteossarcoma (OSA) é a neoplasia óssea primária mais diagnosticada em pacientes caninos. Este trabalho relata o caso de um canino, com seis anos de idade, sem raça definida, fêmea, não castrado, apresentando OSA em região de palato duro. Ao exame clínico, constatou-se uma massa de consistência firme em região palatina. Além do exame clínico, foram solicitadas radiografias torácicas, exames hematológicos e bioquímicos, exames histopatológicos e tomografia computadorizada. Fora então dado o diagnóstico de OSA condroblástico, e o tumor foi caracterizado pela imuno-histoquímica. Não foram observadas evidências de metástases nesse caso. O tratamento instituído consistiu na combinação de quimioterapia convencional, quimioterapia metronômica e cuidados paliativos, almejando maior sobrevida e bem-estar do paciente, uma vez que a excisão cirúrgica não foi possível devido à localização e à extensão do tumor. Neste artigo, apresentou-se uma caracterização do osteossarcoma com localização incomum no palato duro.(AU)

Meningeal osteosarcoma in a dogs brain / Osteossarcoma meningeal no encéfalo de um cão

Osteosarcoma is the most common bone cancer in dogs. It has a high invasion capacity and commonly metastasizes. This neoplasia ensues mainly in the medullary canal of long bones of the appendicular and axial skeleton, rarely affecting primarily extra-skeletal sites. Primary meningeal osteosarcomas are extremely rare both in human and veterinary medicine. A male, mixed breed dog was admitted at the Veterinary Hospital with a history of over excitement, decreased neurological reflexes, and seizures. The worsening of its clinical condition led to its euthanasia and anatomopathological examination at the Veterinary Pathology Service. This report describes a primary extra-skeletal osteosarcoma of a dogs meninges. Its clinical signs were indicative of a central nervous system disease later diagnosed by histopathological and immunohistochemical analysis.(AU)

O osteossarcoma é considerado a neoplasia óssea mais frequente em cães. Tem elevada capacidade invasiva e comumente gera metástase. Essa neoplasia é mais frequentemente observada no canal medular de ossos longos do esqueleto apendicular e axial. É raro que acometa primariamente sítios extraesqueléticos. O osteossarcoma primário meningeal é considerado extremamente raro em medicinas humana e veterinária. Um cão macho e sem raça definida foi atendido no Hospital Veterinário com histórico de excitação, diminuição dos reflexos neurológicos e desenvolvimento de quadro convulsivo. O agravamento do quadro clínico levou à eutanásia do animal e a um exame anatomopatológico pelo Serviço de Patologia Veterinária. O objetivo deste relato é descrever um caso de osteossarcoma extra-esquelético primário de meninge em um cão que apresentou quadro clínico compatível com doença originária do sistema nervoso central e que foi diagnosticado por meio das análises histopatológica e imunohistoquímica.(AU)

Ethanolic extract of gabirobeira (Campomanesia adamantium) leaves reduces proliferation of osteosarcoma cells in vitro

Osteosarcoma is the most commonly diagnosed malignant bone tumor in humans, with a higher incidence in children and young people. It is highly aggressive and has a high metastatic potential. Its treatment is based on both chemotherapy and surgical intervention. However, currently used chemotherapeutic agents, such as doxorubicin, have several adverse effects on the patient. Therefore, there is a growing demand for new chemotherapeutic agents that stimulate new researches, such as those involving compounds extracted from plants, such as the gabirobeira. In this study, we aimed to evaluate the cytotoxic effects of ethanolic extract, both crude and ethyl acetate, of gabirobeira leaves on osteosarcoma cells in vitro. Cytotoxicity was evaluated using the Trypan blue exclusion method and the IC50 values were calculated using the tetrazolium reduction method. The ethanolic extract of gabirobeira leaves showed a cytotoxic effect on osteosarcoma cells in vitro. The group treated with the crude extract at 1. 0& 956;L mL-1 concentration for 48 hours showed higher cytotoxicity and the lowest IC50 value for this extract was found in the 24 to 48 hours interval. The ethanolic extract of gabirobeira leaves is cytotoxic for osteosarcoma cells.

Ethanolic extract of gabirobeira (Campomanesia adamantium) leaves reduces proliferation of osteosarcoma cells in vitro

Osteosarcoma is the most commonly diagnosed malignant bone tumor in humans, with a higher incidence in children and young people. It is highly aggressive and has a high metastatic potential. Its treatment is based on both chemotherapy and surgical intervention. However, currently used chemotherapeutic agents, such as doxorubicin, have several adverse effects on the patient. Therefore, there is a growing demand for new chemotherapeutic agents that stimulate new researches, such as those involving compounds extracted from plants, such as the gabirobeira. In this study, we aimed to evaluate the cytotoxic effects of ethanolic extract, both crude and ethyl acetate, of gabirobeira leaves on osteosarcoma cells in vitro. Cytotoxicity was evaluated using the Trypan blue exclusion method and the IC50 values were calculated using the tetrazolium reduction method. The ethanolic extract of gabirobeira leaves showed a cytotoxic effect on osteosarcoma cells in vitro. The group treated with the crude extract at 1. 0& 956;L mL-1 concentration for 48 hours showed higher cytotoxicity and the lowest IC50 value for this extract was found in the 24 to 48 hours interval. The ethanolic extract of gabirobeira leaves is cytotoxic for osteosarcoma cells.(AU)

Osteossarcoma pélvico metastático em cão / Metastatic pelvic osteosarcoma in a dog

Background: Osteosarcomas are malignant neoplasms of bone tissue, with a high prevalence in dogs, especially in large and giant breeds. More commonly, such alterations affect the appendicular skeleton and, to a lesser extent, the axial skeleton. In order to obtain an accurate diagnosis, it is necessary to combine cytological and histopathological findings with clinical parameters, imaging exams and macroscopic findings. In the present study, we report a rare case of combined-type pelvic osteosarcoma with pulmonary metastasis in a dog. Case: A 5-year-old intact large male dog of mixed breed, was submitted to clinical care because of an increase in volume of the left perineal region. The cytological evaluation, performed without imaging exams, indicated that it was an undifferentiated sarcoma. An incisional biopsy defined the diagnosis as telangiectatic osteosarcoma, and with progressive clinical worsening, the patient died. Necroscopic examination revealed multiple nodules in the lungs and an irregular mass with a hard to friable consistency. The mass was intensely vascularised and extended craniodorsally from the left ischial tuberosity to the base of the renal fossa. Microscopically, the neoplasm was diagnosed as combined osteosarcoma, consisting of the osteoblastic, chondroblastic, and telangiectatic subtypes. Metastases with a predominance of the chondroblastic subtype were observed in the lungs. Discussion: This is the first report of combined-type canine osteosarcoma in the ischium. The case reported here is unusual, as there are few reports of canine osteosarcoma in the pelvic bones, and there is no concrete information regarding its histological appearance. Osteosarcoma is the most common bone neoplasm in dogs, representing up to 80% of the tumours found in such organs. In the present case, the dog was a large young adult with a higher probability of neoplasm development. A cytopathological examination is a diagnostic method with good sensitivity and specificity that can confirm osteosarcomas. However, in this case, the cytological diagnosis, performed without the information from the imaging exam, indicated that it was an undifferentiated sarcoma, given the impossibility of the architectural assessment of the lesion. Biopsy samples sent for histology may not be representative of the entire tumour, leading to misclassification of the histological type. Therefore, the evaluation of fragments from various sites of the lesions is recommended. Regarding the morphology of osteosarcomas, such neoplasms have the osteoblastic, chondroblastic, fibroblastic, telangiectatic, large cell, and poorly differentiated subtypes. With regard to tumours located in the axial skeleton, no studies have assessed the predominance of a particular morphological type, as well as the incidence of combined-type masses in dogs in this particular location. Such neoplasms are locally aggressive and have a high metastatic potential, with the lungs being the main location for implantation of neoplastic cells. There is no proven evidence of the correlation between morphological presentations and the presence of metastases from osteosarcomas in dogs. The histological type is not a predictive factor for the behaviour of the neoplasm. However, the anatomical location is considered as one of the factors with the greatest influence on the prognosis and metastatic potential. Rib masses are associated with a higher rate of metastases compared to others. The definitive diagnosis of osteosarcomas and its correct subclassification are of great importance in the prognosis of affected patients. These require an approach that considers the clinical findings, imaging examinations, and macroscopic and microscopic alterations.

Mediastinal extraskeletal osteosarcoma in a canine with pulmonary and cerebral metastasis

Background: Extraskeletal osteosarcoma (EOS), a rare variant of osteosarcoma (OS), is a malignant neoplasm that develops insoft tissues without primary bone involvement. This study aims to describe a case of EOS with a mediastinal location in a canine.Case: A 10-year-old male Uruguayan Cimarron dog, was presented to the Laboratório Regional de Diagnóstico, Faculdadede Veterinária, Universidade Federal de Pelotas (LRD/FV/UFPel) for necropsy. The dog had a history of submandibularswelling, progressive hind limb paralysis, muscle atrophy, and breathing difficulties. During necropsy, in the thoracic cavity,approximately 900 mL of serosanguinous exudate and a reddish-brown, bossed mediastinal mass measuring 15.0 cm in thelongest axis were also noted. The lung exhibited multifocal to coalescent, white, firm nodules extending from the pleurato the parenchyma and measuring up to 4.5 cm in diameter. In the parietal and occipital region of the brain, a matte winemass measuring 2.3 cm in the longest axis was observed. Fragments of the neoplastic mass, organs of the abdominal andthoracic cavities, and the brain were harvested and fixed in 10% buffered formalin. After 48 h, the samples were routinelyprocessed, incorporated in paraffin, cut into 3 µm-thick sections, and stained using Hematoxylin and Eosin (HE). Selectedsections of the neoplasm, the lung, and the brain were subjected to Von Kossa staining and immunohistochemical (IHC)analysis. For IHC, primary anti-cytokeratin monoclonal antibodies (clone AE1 / AE3, BioCare Medical) at a 1:100 dilution, vimentin (clone V9, BioCare Medical) at a 1:100 dilution, S100 Protein (clone 15E2E2, BioCare Medical) at a 1:100dilution, and Ki67 (SP6 clone, BioCare Medical) at a 1:50 dilution were used. Immunostaining ...

Mediastinal extraskeletal osteosarcoma in a canine with pulmonary and cerebral metastasis

Background: Extraskeletal osteosarcoma (EOS), a rare variant of osteosarcoma (OS), is a malignant neoplasm that develops insoft tissues without primary bone involvement. This study aims to describe a case of EOS with a mediastinal location in a canine.Case: A 10-year-old male Uruguayan Cimarron dog, was presented to the Laboratório Regional de Diagnóstico, Faculdadede Veterinária, Universidade Federal de Pelotas (LRD/FV/UFPel) for necropsy. The dog had a history of submandibularswelling, progressive hind limb paralysis, muscle atrophy, and breathing difficulties. During necropsy, in the thoracic cavity,approximately 900 mL of serosanguinous exudate and a reddish-brown, bossed mediastinal mass measuring 15.0 cm in thelongest axis were also noted. The lung exhibited multifocal to coalescent, white, firm nodules extending from the pleurato the parenchyma and measuring up to 4.5 cm in diameter. In the parietal and occipital region of the brain, a matte winemass measuring 2.3 cm in the longest axis was observed. Fragments of the neoplastic mass, organs of the abdominal andthoracic cavities, and the brain were harvested and fixed in 10% buffered formalin. After 48 h, the samples were routinelyprocessed, incorporated in paraffin, cut into 3 µm-thick sections, and stained using Hematoxylin and Eosin (HE). Selectedsections of the neoplasm, the lung, and the brain were subjected to Von Kossa staining and immunohistochemical (IHC)analysis. For IHC, primary anti-cytokeratin monoclonal antibodies (clone AE1 / AE3, BioCare Medical) at a 1:100 dilution, vimentin (clone V9, BioCare Medical) at a 1:100 dilution, S100 Protein (clone 15E2E2, BioCare Medical) at a 1:100dilution, and Ki67 (SP6 clone, BioCare Medical) at a 1:50 dilution were used. Immunostaining ...(AU)

Metastatic osteoblastic osteosarcoma in a captive scarlet macaw (Ara macao)

Osteosarcoma is a malignant primary tumor of the bone, which is considered rare in birds. This report describes an osteoblastic osteosarcoma in a scarlet macaw (Ara macao), which was apathetic with progressive weight loss, and had a tumor in the distal portion of the femur and proximal tibiotarsus with ulcerated pododermatitis in the contralateral limb. Euthanasia was elected due to poor diagnosis after radiographic and cytological examination. Histopathology and immunohistochemistry were performed and confirmed the diagnosis of an osteoblastic osteosarcoma with hepatic and pulmonary metastases.(AU)

Metastatic osteoblastic osteosarcoma in a captive scarlet macaw (Ara macao)

Osteosarcoma is a malignant primary tumor of the bone, which is considered rare in birds. This report describes an osteoblastic osteosarcoma in a scarlet macaw (Ara macao), which was apathetic with progressive weight loss, and had a tumor in the distal portion of the femur and proximal tibiotarsus with ulcerated pododermatitis in the contralateral limb. Euthanasia was elected due to poor diagnosis after radiographic and cytological examination. Histopathology and immunohistochemistry were performed and confirmed the diagnosis of an osteoblastic osteosarcoma with hepatic and pulmonary metastases.

Fibroblastic osteosarcoma in a white-eared opossum (Didelphis albiventris) / Osteossarcoma fibroblástico em gambá-de-orelha-branca (Didelphis albiventris)

Osteosarcoma is characterized by the production of osteoid or woven bone, using neoplastic osteoblasts. It is the most common primary bone neoplasm in canines and humans. This neoplasm was previously reported in all vertebrate classes, including a wide variety of mammals. However, there is no case report describing this neoplasm in Didelphis albiventris. Therefore, the objective of this manuscript is to describe the clinical-pathological aspects of fibroblastic osteosarcoma in D. albiventris. A wild adult male white-eared opossum (D. albiventris) arrived at the zoological park of the "Universidade de Caxias do Sul" with a swollen left thoracic limb. After a general clinical examination, the animal was transferred to the Veterinary Clinic of "Universidade de Caxias do Sul" for radiographic examination of the left thoracic limb and thorax. Additionally, some material was collected through fine needle aspiration (FNA) for cytologic evaluation. The radiographic findings and cytologic evaluation indicated osteosarcoma. The animal was euthanized due to severe clinical conditions and guarded prognosis. During necropsy, macroscopic analysis of the viscera was performed, fragments of various organs were collected and fixed in 10% neutral buffered formalin. All fragments were processed following routine histological techniques. The histopathological evaluation confirmed osteosarcoma, which was classified as a fibroblastic subtype. Case reports are crucial for the knowledge of incidence, prevalence, and behavior of the current mentioned disease, as well as other diseases, in species with such limited information. In order to obtain a decisive diagnosis, a few different examination methods were associated. Although the observations presented are based on a single case, this neoplasm had a similar clinical presentation to that described in other species.(AU)

O osteossarcoma é caracterizado pela produção de osteoide ou osso imaturo, por osteoblastos neoplásicos. É a neoplasia óssea primária mais comum em caninos e humanos. Essa neoplasia já foi relatada em todas as classes de vertebrados, incluindo uma grande variedade de mamíferos. Não havendo descrição dessa neoplasia até o momento em Didelphis albiventris. O objetivo deste trabalho é descrever aspectos clínico-patológicos de um caso de osteossarcoma fibroblástico em D. albiventris. Chegou para atendimento no Zoológico da Universidade de Caxias do Sul um gambá-de-orelha-branca (D. albiventris), macho, adulto, de vida livre com aumento de volume no membro torácico esquerdo. Após avaliação clínica geral, o animal foi encaminhado para a Clínica Veterinária da Universidade de Caxias do Sul para realização de radiografia do membro torácico esquerdo e de tórax, sendo também realizada coleta de material por punção aspirativa por agulha fina (PAAF) para avaliação citológica. Os achados radiográficos e da avaliação citológica foram sugestivos de osteossarcoma. Devido ao estado clínico grave e prognóstico reservado optou-se pela eutanásia. Durante a necropsia realizou-se a análise macroscópica das vísceras, foram coletados fragmentos de diversos órgãos, fixados em formalina 10%, processados pelas técnicas histológicas de rotina. Na avaliação histopatológica confirmou-se a suspeita de osteossarcoma sendo classificado no subtipo fibroblástico. A descrição de relatos é fundamental para conhecimento da incidência, prevalência e comportamento desta e de outras doenças em espécies que as informações são limitadas. A associação de diferentes métodos de exames foram necessários para a obtenção de um diagnóstico definitivo. Embora as observações apresentadas se baseiem em um caso único, esta neoplasia possuiu apresentação clínica semelhante a descrita em outras espécies.(AU)