Quantification of adhesion of mesenchymal stem cells spread on decellularized vein scaffold
Rodrigues, Lenize da Silva; Bovolato, Ana Lívia de Carvalho; Silva, Bárbara Esteves; Chizzolini, Leticia Victória; Cruz, Bianca Latance da; Moraes, Marcelo Padovani de Toledo; Lourenção, Pedro Luiz Toledo de Arruda; Bertanha, Matheus.
Acta cir. bras;
36(10): e361001, 2021. graf
Artigo
em Inglês
| LILACS
| ID: biblio-1345021
Resumo
ABSTRACT Purpose: To evaluate methods that improve adipose-derived stem cells (ASCs) population in decellularized biological venous scaffold for tissue engineering in blood vessels, a model in rabbits. Methods: The ASC was expanded until the third passage. Inferior vena cava (IVC) was submitted to the decellularization process using 1% sodium dodecyl sulfate (SDS) or 2% sodium deoxycholate (SD) to compose 12 study groups (G): pure SD or SDS, exposed or not to 1% TritonX-100 (TX-100) and exposed or not to poly-l'lysine and laminin (PL). Scaffolds were covered with 1 × 105 or 1 × 106 ASCs diluted in 10 μL Puramatrix™. The histological analysis was done by cell counting in hematoxylin and eosin (HE) and nuclei count in immunofluorescence (IF) with 4',6-Diamidine-2'-phenylindole dihydrochloride (DAPI). Results: The study of groups in HE and IF showed similar results. For both analyses,IVC-SD-1 × 106 ASC and IVC-SD-PL-1 × 106 ASC provided the best results. The IF technique showed better sensitivity than HE, with a weak agreement between them. Conclusions: Decellularizing agent and the number of ASC influence scaffolds cellularization response and the best protocols as those ones using SD with or without the addition of PL.
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BR1.1
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