Is the peptide-mediated gene delivery a feasible and reliable technology for efficient transgenesis?
Radis-Baptista, Gandhi.
Acta sci. vet. (Impr.);
38(supl.2): s605-s613, 2010. ilus, tab
Artigo
em Inglês
| VETINDEX
| ID: biblio-1411908
Resumo
Background: Cell penetrating peptides (CPPs) are natural or synthetic, cationic or amphipathic short peptide sequences with high affinity for biological membranes, capable of crossing the lipid bilayer systems and mediating the delivery of biomolecules, drugs or nanoparticles into the cells. Review: In this work, I presented a brief overview of some significant contributions from several groups around the world dealing with the theme. Our own groups succeeded in characterizing natural and synthetic peptides with cell penetration properties. These peptides and derived sequences were found in natural proteins, like animal toxins, and improved by rational design and chemical synthesis. For instance, peptide derivatization of crotamine a cationic and defensin-like peptide found in the venom of South American rattlesnake (Crotalus durissus terrificus) allowed us to produce shorter derived and analogs with improved nuclear homing property and intrinsic nucleolar targeting capabilities. Conclusion: The potential of biotechnological and biomedical applications of natural or synthetic CPPs, for the field of cell biology could include: the fluorescent image analysis of subcellular and organellar structures; the delivery of hydrophilic drugs and antiproliferative agents to treat cancer and degenerative diseases; the delivery of biomolecules into the mammalian cells for cancer treatment, cell and gene therapy, gene silencing and transgenesis.
Biblioteca responsável:
BR68.1
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