Chicken recombinant adiponectin enhances fatty acid metabolism in oleic acid- and palmitic acid-treated LMH cells
Zhuang, Yi-Ru; Lin, Yuan-Yu.
Rev. bras. zootec;
51: e20220087, 2022. ilus, tab, graf
Artigo
em Inglês
| VETINDEX
| ID: biblio-1442799
Resumo
The objective of this study was to investigate the effects of recombinant adiponectin on chicken liver cells. The full-length chicken adiponectin gene was amplified by PCR and cloned into the vector pET-32a, followed by the transformation of the vector into Escherichia coli BL21. SDS-PAGE was used to detect and analyze the purity of the expressed recombinant protein. Induction was performed with 1 mM IPTG at 30 °C for 3 h, and the recombinant thioredoxinadiponectin fusion protein was purified using Ni-NTA affinity chromatography. Chicken adiponectin was successfully expressed and purified in a bacterial system. In addition, the chicken recombinant adiponectin demonstrated that it ameliorates palmitic acid- and oleic acid-induced adipogenesis, in which an increase in ß-oxidation and a decrease in lipogenesis-related genes may be involved. In summary, chicken recombinant adiponectin enhances fatty acid metabolism in LMH cells.(AU)
Biblioteca responsável:
BR68.1
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