Isoflavones hydrolisis and extraction / de Isoflavonas
Fernandes Farias dos Santos, Jozilene; Maria Roncato Duarte, Keila; Ferreira Pessoa, Fernanda; Atauri Cardelli de Lucena, Marcia.
Bol. ind. anim. (Impr.);
69: 47-47, 2012.
Artigo
em Português
|
LILACS-Express
| ID: biblio-1466483
Resumo
Isoflavones are found in leguminous species and are used as phytoestrogens widely used by industry for its beneficial effects as estrogens mimicked, antioxidant action and anti-cancer activity. The identification and quantification of isoflavones in plants is a need due to the high demand of industry. Several methods are used for its extraction, using organic solvents (methanol, ethanol and acetonitrile). Samples from five legumes species from Instituto de Zootecnia (IZ), Forage Gene Bank were tested. All seeds received a hydrothermic treatment immersed in pure water at 50C for 12 hours. Seeds were then oven-dryed. In this work we tested the extraction using only the hydrothermic treatment and hyfrothermic treatment allied to methanol extaction protocol. Seeds were grinded and half of the samples were ressuspended in PBS (phosphate Buffer) and the other half were submited to 4 mL of methanol and 1% of acetic acid, soaked for 5 hours, shaked every 15 minutes, at room temperature. The five legume species that we quantify isoflavones by enzyme immunoassay (EIA) were: Calopogonium mucunoides, Bauhinia sp., Cajanus cajan, Galactia martii, Leucaena leucocephala. The extraction procedure is a recomendation of AOAC (Association of Official Analytical Chemists) for isoflavone quantification. Ours results show an increase of extraction using methanol 80% plus acetic acid 1% and was obtaO artigo não apresenta resumo em português.
Biblioteca responsável:
BR68.1
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