Samsum ant venom modulates the immune response and redox status at the acute toxic dose in vivo
Ebaid, Hossam; Abdel-Salam, Bahaa; Alhazza, Ibrahim; Al-Tamimi, Jameel; Hassan, Iftekhar; Rady, Ahmed; Mashaly, Ashraf; Mahmoud, Ahmed; Sammour, Reda.
J. venom. anim. toxins incl. trop. dis;
25: e.20190020, 2019. ilus, tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-1484762
Resumo
Background:Ant venoms express surface molecules that participate in antigen presentation involving pro- and anti-inflammatory cytokines. This work aims to investigate the expression of MHC-II, CD80 and CD86 on the polymorphonuclear cells (PMNs) in rats injected with samsum ant venom (SAV).Methods:Rats were divided into three groups - control, SAV-treated (intraperitoneal route, 600 μg/kg), and SAV-treated (subcutaneous route, 600 μg/kg). After five doses, animals were euthanized and samples collected for analysis.Results:The subcutaneous SAV-trated rats presented decreased levels of glutathione with increased cholesterol and triglyceride levels. Intraperitoneal SAV-treated animals displayed significantly reduced concentrations of both IFN-γ and IL-17 in comparison with the control group. However, intraperitoneal and subcutaneous SAV-treated rats were able to upregulate the expressions of MHC-II, CD80 and CD86 on PMNs in comparison with the control respectively. The histological examination showed severe lymphocyte depletion in the splenic white pulp of the intraperitoneal SAV-injected rats.Conclusion:Stimulation of PMNs by SAV leads to upregulation of MHC-II, CD 80, and CD 86, which plays critical roles in antigen presentation and consequently proliferation of T-cells. Subcutaneous route was more efficient than intraperitoneal by elevating MHC-II, CD80 and CD86 expression, disturbing oxidative stability and increasing lipogram concentration.
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BR68.1
Localização: BR68.1
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