Blanching effect on the bioactive compounds and on the viability of Lactobacillus rhamnosus GG before and after in vitro simulation of the digestive system in jabuticaba juice / Efeito do branqueamento nos compostos bioativos e na viabilidade de Lactobacillus rhamnosus GG antes e após simulação in vitro do sistema digestivo em suco de jabuticaba
Oliveira, Daniane Campos de; Martins, Eliane Maurício Furtado; Martins, Maurílio Lopes; Martins, Giovanna Bretas; Binoti, Mirella Lima; Campos, André Narvaes da Rocha; Ramos, Afonso Mota; Silva, Maurício Henriques Louzada; Stringheta, Paulo César.
Semina ciênc. agrar;
38(3): 1277-1294, maio-jun. 2017. graf, tab
Artigo
em Inglês
| VETINDEX
| ID: biblio-1500766
Resumo
The viability of Lactobacillus rhamnosus GG (LGG) in jabuticaba juices and its survival in the gastrointestinal tract (GIT), simulated in vitro, was studied. Two juices were prepared: A with nonblanched fruits, and B with blanched fruits. LGG was then added and the juices maintained at 8 ºC for 28 days. The control treatment consisted of juices without the added probiotic. The following were determined in the juices: the viability and in vitro survival of LGG, fecal coliforms, Salmonella sp., pH, acidity, total soluble solids (TSS), color, antioxidant capacity, total phenolic compounds, anthocyanins and ascorbic acid. The sensory acceptability was also determined using a 9-point hedonic scale. Blanching interfered (p < 0.05) with the viability of LGG, juice A showing the greatest viability as compared to juice B. After in vitro simulation, the probiotic bacterial count was < 1.0 log CFU mL-1, which demonstrates the low resistance of the strain to the simulated GIT conditions. The juices were conformed to the microbiological standards established by law. The pH, acidity and TSS were influenced by blanching (p < 0.05), with values of 5.03, 0.46% and 15.38 °Brix for juice A and 5.12, 0.66% and 16.05 °Brix for juice B, respectively. The addition of LGG did not influence these characteristics. Only the pH value was influenced by the storage time (p < 0.05), increasing throughout storage. Juice B showed lower luminosity (L*) and a greater value for a* as compared to juice A, indicating that the former became darker and redder due to the blanching process. Both juices showed positive values for the b* coordinate. The juice was found to be a good source of polyphenols. Neither the time nor the addition of LGG affected the antioxidant capacity, total phenolic compounds or anthocyanin contents...A viabilidade de Lactobacillus rhamnosus GG (LGG) em sucos de jabuticaba e sua sobrevivência ao trato gastrointestinal (TGI) simulado in vitro foram estudadas. Foram preparados dois sucos: A (com frutas não branqueadas) e B (com frutas branqueadas), os quais foram adicionados de LGG e mantidos a 8 °C durante 28 dias. O tratamento controle consistiu dos sucos sem adição de probiótico. Determinouse a viabilidade e a sobrevivência in vitro de LGG nos sucos, coliformes termotolerantes, Salmonella sp., pH, acidez, sólidos solúveis totais (SST), cor, capacidade antioxidante, compostos fenólicos totais, antocianinas totais e ácido ascórbico, além da aceitabilidade em escala hedônica de 9 pontos. O branqueamento interferiu (p 0,05). Os sucos B apresentaram menor luminosidade (L*) e maior valor de a* com coloração mais escura e avermelhada. Ambos os sucos apresentaram valores positivos para a coordenada b*. Os sucos revelaram ser fonte de compostos fenólicos como antocianinas, responsáveis pela considerável capacidade antioxidante dos produtos. Não foi observado efeito do tempo e da adição de LGG na capacidade antioxidante, compostos fenólicos totais e antocianinas...
Biblioteca responsável:
BR68.1
Localização: BR68.1
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