Validation of the CellRox Deep Red ® fluorescent probe to oxidative stress assessment in equine spermatozoa
Lançoni, Renata; Arruda, Rubens Paes de; Alves, Maíra Bianchi Rodrigues; Oliveira, Letícia Zoccolaro; Santos, Gabriel De Carli dos; Lemes, Kleber Menegon; Florez-Rodriguez, Andréa; Celeghini, Eneiva Carla Carvalho.
Anim. Reprod.;
14(2): 437-441, 17e.2017e.2017. graf, tab, ilus
Artigo
em Inglês
| VETINDEX
| ID: vti-15959
Resumo
Considering the importance of ROS influence on sperm functionality and some limitations in sperm oxidative stress assessment methods, a field to studies of new techniques are still open. In this sense, the aim of this study is to validate the ROS detection technique through the CellRox Deep Red Reagent®probe in stallion sperm. Four stallions were used and the analyses were conducted on four replicates of semen samples from each of stallion (n = 16). The results of the polynomial regression presented a quadratic effect, high determination coefficient value (R2 = 0.88) and high significant P value (P < 0.0001). The CellRox Deep Red® fluorescent probe is able to detect reactive oxygen species in equine sperm, indicating accurately the occurrence of oxidative stress in stallion semen.(AU)
Biblioteca responsável:
BR68.1
Localização: BR68.1
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