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Ci. Rural; 45(5): 892-897, 2015.

Artigo em Inglês | VETINDEX | ID: vti-16029

Resumo

Salminus brasiliensis is a migratory fish that has attracted considerable interest for aquaculture. Several procedures for induced spawning are known; however, there is a lack of protocol which enables the use of cryopreserved semen. Therefore, this study was conducted to investigate the use of cryopreserved semen using different volumes of cryopreserved semen relative to oocytes, different activators solutions and different maintenance time during the fertilization of dourado to evaluate the impact of these parameters on the fertilization rate. The semen was collected, cryopreserved in 0.5mL straws and stored in a dry shipper. Oocytes samples were fertilized according to each treatment. The different activator solutions and the contact times of the gametes with activators affected significantly the fertilization rates, which ranged between 13.4 and 27.8%, while fresh semen fertility rate was 80.8%. The relationship between oocyte and cryopreserved semen was significant, being the best ratio 0.05mL of cryopreserved semen per 10g of oocytes, while upper or lower volumes promoted a reduction in fertilization. The use of cryopreserved semen was effective to fertilize S. brasiliensis oocytes, however produced lower fertility rate than fresh semen

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O dourado, Salminus brasiliensis, ; um peixe migrador que tem despertado interesse para piscicultura. Os procedimentos convencionais para a sua reprodu;;o s;o conhecidos, contudo, falta um protocolo para o uso do s;men criopreservado. Dessa forma, objetivou-se avaliar diferentes volumes de s;men criopreservado, ouso de diferentes solu;;es ativadoras e com diferentes tempos de contato do s;men com os ov;citos na taxa de fertiliza;;o. Para tal, o s;men foi coletado e criopreservado em palhetas de 0,5mL em vapor de nitrog;nio l;quido. Amostras de ov;citos foram fertilizadas conforme os distintos tratamentos. As diferentes solu;;es testadas e o tempo de contato dos ativadores afetaram significativamente as taxas de fertiliza;;o, com valores que variaram entre 13,4 e 27,8%, enquanto o s;men fresco propiciou 80,8% de taxa de fertiliza;;o. O volume de s;men criopreservado afetou a taxa de fertiliza;;o dos ovos, sendo 0,05mL para 10g de ov;citos, o que promoveu os melhores resultados, sendo que volumes superiores e inferiores promoveram redu;;o na fertiliza;;o. O uso de s;men criopreservado foi efetivo na fertiliza;;o dos ov;citos de dourado, no entanto, foram obtidas taxas de fertiliza;;o inferiores ;quelas observadas com o uso de s;men fresco

Biblioteca responsável: BR68.1