Acute hyperglycemia prevents dexmedetomidine-induced preconditioning against renal ischemia-reperfusion injury
Wang, Huaxin; Chen, Hui; Wang, Lei; Liu, Lin; Wang, Min; Liu, Xiuheng.
Acta cir. bras.;
29(12): 812-818, Dec. 2014. tab, graf, ilus
Artigo
em Inglês
| VETINDEX
| ID: vti-18836
Resumo
PURPOSE:To investigate the effects of acute hyperglycemia on dexmedetomidine-induced preconditioning against renal ischemia-reperfusion injury.METHODS:Sprague-Dawley rats were randomly arranged to the normoglycemic (NG) or hyperglycemic group (HG), with each group further divided into sham (no I/R injury), I/R (ischemia-reperfusion) and dex (given by dexmedetomidine) groups. Acute hyperglycemia was induced by intraperitoneal injection (i.p.) of 25% glucose (3 g/kg) 45 min before ischemia. Dexmedetomidine (50 μg/kg, i.p.) was administrated 30 min before induction of ischemia. Renal function, histology, apoptosis, expression of Bax, Bcl-2 and phosphorylated AKT (p-AKT) were detected.RESULTS:I/R insult significantly increased the serum levels of blood urea nitrogen and creatinine, apoptotic tubular epithelial cells, expression of Bax and p-AKT, but decreased Bcl-2 expression. All these changes were further enhanced by hyperglycemia (p<0.05). In hyperglycemic condition, there was no statistically difference between the I/R group and Dex group in all the aforementioned detection indexes (p>0.05).CONCLUSION:Acute hyperglycemia attenuates dexmedetomidine-induced preconditioning against renal ischemia-reperfusion injury in non-diabetic rats.(AU)
Biblioteca responsável:
BR68.1
Localização: BR68.1
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