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Comparação de testes sorológicos e molecular para diagnóstico da Artrite Encefalite Caprina e avaliação clínica da glândula mamária de caprinos leiteiros infectados / Comparison of serological and molecular tests for diagnosis of Caprine Arthritis Encephalitis and clinical evaluation of mammary glands of infected dairy goats

Azevedo, Dalva Alana Aragão de; Pinheiro, Raymundo Rizaldo; Santos, Vanderlan Warlington Souza dos; Damasceno, Edgar Marques; Sousa, Ana Lídia Madeira de; Araújo, Juscilânia Furtado; Andrioli, Alice; Sider, Lúcia Helena; Peixoto, Renato Mesquita; Teixeira, Maria Fátima da Silva.
Acta sci. vet. (Online); 47: Pub. 1668, June 25, 2019. tab, graf
Artigo em Português | VETINDEX | ID: vti-21138


Background: Caprine Arthritis Encephalitis (CAE) is a disease that causes productive losses in dairy goat flocks due to thereduction in milk production, followed by lesions in joints and mammary glands. An early diagnosis is essential, consideringthat there is frequent occurrence of asymptomatic animals. Hence, this study aimed to perform a comparison of immunological and molecular based diagnostic tests, represented by Agar Gel Immunodiffusion (AGID), Western Blot (WB) andnested Polymerase Chain Reaction (nPCR). In addition, the mammary glands (MG) of dairy goats were clinically evaluated.Materials, Methods & Results: Blood collection and clinical examination were performed in 1191 dairy goats of 12 farms locatedin Northeastern and Southeastern regions of Brazil. Serological (AGID, WB) and molecular (nPCR) test results were comparedand the data, along with MG alterations, were analyzed using Epi-info 7 and WinEpiscope 2.0. Seroprevalence in AGID test was41.14% (490/1191). In WB, 51.47% (613/1191) of animals were seropositive and nPCR detected 69.44% (827/1191) positiveanimals. Hence, WB was more sensitive (P < 0.001) than AGID. However, nPCR detected more positive animals than AGID (P< 0.001) and WB (P < 0.001). The analysis of mammary glands revealed that 105 out of 1096 nanny goats presented alterations,of which 101 presented altered consistency, 16 presented elevated temperatures and 60 had enlarged retromammary lymph nodes.There was significant statistic difference (P < 0.05) only when comparing the results of serological tests with MG alterations.Discussion: In general, AGID technique is most frequently used when screening flocks for the disease due to the practicalityand low cost this test presents. However, the results demonstrated that AGID detected the lowest number of positive animals.This low sensitivity that the test presented may be attributed to its antigen-antibody interaction mechanism...(AU)
Biblioteca responsável: BR68.1