Efeito de diferentes meios e concentrações espermáticas na fecundação in vitro curta sobre a produção de embriões bovinos / Effect of different mediums and sperm concentrations in the short-in vitro fertilization on the bovine embryo production
Cavalcanti, Camila Muniz; Campelo, Iana Sales; Silva, Mirelly Mirna Alves de Sousa; Melo, Luciana Magalhães; Teixeira, Dárcio Ítalo Alves; Freitas, Vicente José de Figueirêdo.
R. bras. Reprod. Anim.;
40(4): 246-247, Out-Dez. 2016. tab
Artigo
em Português
| VETINDEX
| ID: vti-24029
Resumo
tThe present study investigated the effect of two different mediums (IVF-TALP and BO) and spermconcentrations (16 106 and 20 106), during the short-in vitro fertilization (IVF) on bovine embryoproduction. Thus, after in vitro maturation (IVM), oocytes were randomly distributed into three groups:traditional IVF (control), IVF-TALP and Brackett-Oliphant (BO). The groups IVF-TALP and BO weresubdivided according sperm concentration (16 106 and 20 106) used for short-IVF (6 h). For the controlgroup, the IVF was performed for 18 h. Concerning the cleavage rate, the BO-20 group showed the best results(77,1%). On the other hand, for the blastocyst yield, the highest results were obtained in control and BO-20groups: 20.8% and 24.6%, respectively. In conclusion, it seems that BO medium with 20 106 sptz/mL positivelyinfluence the bovine embryo in vitro production. However, further studies should be performed.(AU)
Biblioteca responsável:
BR68.1
Localização: BR68.1
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