Supplemented powdered coconut water (ACP-406®) promotes growth of goat secondary follicles and oocyte meiotic resumption
Pontes, Luana Mirela de Sales; Gouveia, Bruna Bortoloni; Menezes, Vanúzia Gonçalves; Barros, Vanessa Raquel Pinto de; Barberino, Ricássio de Sousa; Monte, Alane Pains Oliveira do; Donfack, Nathalie Jiatsa; Celestino, Juliana Jales de Hollanda; Salgueiro, Cristiane Clemente de Mello; Figueiredo, José Ricardo de; Matos, Maria Helena Tavares de.
Anim. Reprod.;
16(4): 819-828, 2019. ilus, tab, graf
Artigo
em Inglês
| VETINDEX
| ID: vti-24100
Resumo
The objective of this study was to test the efficiency of powdered coconut water (ACP-406®) base-medium without or with the addition of supplements on in vitro culture of isolated goat secondary follicles. Follicles were cultured for 18 days in α-MEM or in ACP-406®, both without supplements (referred to as α-MEM and ACP, respectively), or both supplemented with BSA, insulin, transferrin, selenium, glutamine, hypoxanthine, and ascorbic acid (referred to as α-MEM+ and ACP+). Follicular morphology, antrum formation, follicular and oocyte diameter, levels of glutathione (GSH), and chromatin configuration after in vitro maturation were evaluated. At the end of culture, ACP-406® base-medium (without or with supplements) showed a higher (P < 0.05) percentage of normal follicles than α-MEM (without or with supplements). Antrum formation was similar among α-MEM+, ACP and ACP+, and significantly higher than α-MEM without supplements. The follicular diameter was greater in ACP+ than α-MEM, and similar to other treatments. Moreover, fully and daily grown rates were higher (P < 0.05) in ACP-406® base-medium (without or with supplements) than α-MEM (without or with supplements). Levels of GSH were similar between ACP+ and α-MEM+ treatments. Both ACP+ and α-MEM+ allowed meiotic resumption without a significant difference between the two groups. In conclusion, supplemented ACP-406® base-medium maintained follicular survival and promoted the development as well as meiotic resumption of isolated goat secondary follicles cultured in vitro for 18 days.(AU)
Biblioteca responsável:
BR68.1
Localização: BR68.1
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