A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases
Angelini, Leticia Mara Lima; Silva, Amanda Ribeiro Martins da; Rocco, Lucas de Freitas Coli; Milagre, Cintia Duarte de Freitas.
Braz. J. Microbiol.;
46(1): 113-116, Jan.- Mar. 2015. tab, graf
Artigo
em Inglês
| VETINDEX
| ID: vti-481370
Resumo
A modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects.(AU)
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BR1.1
Localização: BR68.1
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