Portal de Pesquisa da BVS Veterinária

Botucatu; s.n; 2006. 116 p.

Tese em Português | VETTESES | ID: vtt-8760

Resumo

O presente trabalho teve por objetivo avaliar os constituintes bioquímicos e protéicos do plasma seminal e séricos de eqüinos, assim como as proteínas da membrana plasmática dos espermatozóides, a fim de estabelecer correlação entre estes dados e a congelabilidade do sêmen na espécie eqüina. Para tanto, foram utilizados 22 animais de sêmen fresco com características normais, dos quais foram feitas colheitas de sêmen para obtenção de amostras de sêmen para congelação, de plasma seminal e de proteínas de membrana e colheitas de sangue para obtenção de soro. Para determinar o perfil bioquímico do plasma seminal e do soro, foram feitas dosagens de glicose, cloretos, sódio, potássio, fósforo, magnésio, colesterol, albumina, proteínas totais, selênio e zinco. O perfil protéico das amostras de plasma seminal, soro sangüíneo e do extrato de proteínas de membranas espermáticas foi determinado por eletroforese unidimensional em gel de poliacrilamida (SDS-PAGE). Após a descongelação e avaliação computadorizada de motilidade e da integridade de membrana das amostras de sêmen congelado, os garanhões foram separados em três grupos estatisticamente distintos, de acordo com a motilidade total

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The aim of the present study was to evaluate biochemical and proteic components of seminal plasma and blood serum and sperm membrane proteins, in order to correlate these findings and the quality of frozen-thawed semen in horses. For this, 22 animals of normal raw semen quality were submitted to semen and blood collection to obtain semen samples to freeze, samples of seminal plasma, samples of membrane proteins and samples of blood serum. To determine biochemical profile of seminal plasma and serum, dosages of glucose, chlorides, sodium, potassium, phosphorus, magnesium, cholesterol, albumin, total proteins, selenium and zinc were performed. Protein profiles of seminal plasma, blood serum and sperm membrane extracts were determined by one-dimensional electrophoresis in polyacrylamide gels (SDS-PAGE). After thawing and evaluation of computerized motility and membrane integrity of thawed semen samples, the stallions were separated into 3 statistically distinct groups, according to total motility of thawed semen, being so classified: good, medium and poor freezers. Biochemical analysis showed difference among three groups only on phosphorus levels, which was higher on poor freezers and was negatively correlated to semen freezability. Electrophoresis of seminal plasma revealed 29 protein bands, of which two (54,5kDa and 40,2kDa) were present in quantity significantly higher on poor freezers and eight were negatively correlated to semen freezability (170,9kDa, 62,2kDa, 54,5kDa, 40,2kDa, 35,2kDa, 19,5kDa, 16,5kDa and 15kDa). Serum electrophoresis revealed 18 protein bands and only one was different among groups (37,6kDa), being significantly lower in poor freezers. Five serum protein bands (124,2kDa, 92,1kDa, 73,9kDa, 37,6kDa and 34,6kDa) were positively correlated to semen freezability. Sperm membrane proteins electrophoresis showed 43 bands, of which three (28,5kDa, 26,3kDa and 21,1kDa) were positively correlated

Biblioteca responsável: BR68.1