RESUMO
Ground beetle data were generated using pitfall traps in the 17-year period from 1993 to 2009 and used to investigate the effects of changes in surrounding crop cover on beetle activity and assemblages, together with the effects of weather variability. Beetles were recorded from non-crop field margins (overgrown hedges). Crop cover changes explained far more variation in the beetle assemblages recorded than did temperature and rainfall variation. A reduction in management intensity and disturbance in the crops surrounding the traps, especially the introduction and development of willow coppice, was concomitant with changes in individual species activity and assemblage composition of beetles trapped in non-crop habitat. There were no consistent patterns in either overall beetle activity or in the number of species recorded over the 17-year period, but there was a clear change from assemblages dominated by smaller species with higher dispersal capability to ones with larger beetles with less dispersal potential and a preference for less disturbed agroecosystems. The influence of surrounding crops on ground beetle activity in non-crop habitat has implications for ecosystem service provision by ground beetles as pest predators. These results are contrary to conventional assumptions and interpretations, which suggest activity of pest predators in crops is influenced primarily by adjacent non-crop habitat. The long-term nature of the assessment was important in elucidation of patterns and trends, and indicated that policies such as agri-environment schemes should take cropping patterns into account when promoting management options that are intended to enhance natural pest control.
Assuntos
Besouros/fisiologia , Produtos Agrícolas/crescimento & desenvolvimento , Animais , Besouros/classificação , Ecossistema , Temperatura , Tempo (Meteorologia)RESUMO
Powdery mildew is a serious economic problem in Mediterranean tomato production. The disease is currently controlled by fungicides (especially sulfur) in both conventional and organic production. Four factorial greenhouse experiments were conducted in successive cropping seasons (autumn 2005, autumn 2006, spring 2006, and spring 2007) to assess the main and combined effects of (i) a less-susceptible hybrid ('Elpida'), (ii) chitin soil amendment, or (iii) Milsana or chitosan elicitor foliar treatments on the severity of powdery mildew and crop performance. Analysis of variance revealed significant effects of cropping season on disease severity and crop yield. All control practices reduced disease severity; reductions of approximately 40, 30, 15, and 15% were achieved by Milsana foliar treatment, the use of a less-susceptible hybrid, chitosan foliar treatment, and chitin soil amendment, respectively. In the season with the highest disease pressure, higher yields were obtained with the less-susceptible hybrid Elpida whereas, in the three seasons with no or low disease pressure, the use of the more susceptible hybrid 'Bison' produced higher yields. Milsana and sulfur treatments did not significantly affect yield (yields were slightly lower compared with untreated control plants) and, therefore, their use cannot be recommended based on the results obtained.
RESUMO
Activity of 12 beneficial invertebrate groups was assessed in 2005 and 2006 on a farm in northern England split into conventional and organic management halves, using pitfall and pan traps set in both crops and field boundaries. Management, crop and boundary structure influences on invertebrate activity were assessed, as was the relationship between crop and boundary type. Classification of crop and boundary assemblages produced three and two groups, respectively, in both years. Organic arable crops had well-defined assemblages in both years; and, while grass and grass/clover fields were separated from conventional arable fields in 2005, there was mixing in 2006. One boundary group, in both years, was dominated by conventional arable fields with tall herbaceous boundary vegetation. The other group had more organic arable and grassy fields with shorter boundary vegetation. Redundancy analyses showed that a number of groups (Cantharidae, Coccinellidae, Syrphidae, Ichneumonidae, Braconidae, Proctotrupoidea, Lycosidae) were more active in organic arable fields with more Staphylinidae in conventional arable crops and no obvious trend with Carabidae, Hemiptera, Neuroptera and Linyphiidae. Activity of some groups, especially Coccinellidae, Syrphidae and parasitic wasps, was strongly related to weed cover. Staphylinidae were most active in tall herbaceous boundaries by conventional arable crops with more of a number of groups (Cantharidae, Coccinellidae, parasitic wasps) in short herbaceous boundaries by organic arable crops. Organic management produced most differences in aerially-dispersed invertebrates, and management had a profound effect on activity in field boundaries. Possible management prescriptions to increase invertebrate activity include changing sowing times, weed cover manipulation and field boundary and margin management.
Assuntos
Agricultura , Conservação dos Recursos Naturais/métodos , Insetos/fisiologia , Aranhas/fisiologia , Animais , Biodiversidade , Inglaterra , Agricultura Orgânica , Plantas Daninhas , Poaceae , TrifoliumRESUMO
Functionally distinct subsets of hippocampal inhibitory neurons exhibit large differences in the frequency, pattern and short-term plasticity of GABA release from their terminals. Heterogeneity is also evident in the ultrastructural features of GABAergic axon terminals examined in the electron microscope, but it is not known if or how this corresponds to interneuron subtypes. We investigated the feasibility of separating morphologically distinct clusters of terminal types, using the approach of measuring several ultrastructural parameters of GABAergic terminals in the CA1 area of the rat hippocampus. Septo-hippocampal axon terminals were anterogradely labeled by biotinylated dextran amine and visualized by pre-embedding immunogold staining to delineate one homogeneous terminal population. Long series (100-150) of ultrathin sections were cut from stratum oriens and stratum radiatum of the CA1 area, and GABAergic terminals were identified by post-embedding immunogold staining. Stereologically unbiased samples of the total GABAergic axon terminal population and a random sample of the septal axon terminals were reconstructed in 3D, and several of their parameters were measured (e.g. bouton volume, synapse surface, volume occupied by vesicles, mitochondria volume). Septal terminals demonstrated significantly larger mean values for most parameters than the total population of local GABAergic terminals. There was no significant difference between terminals reconstructed in the basal and apical dendritic regions of pyramidal cells, neither for the septal nor for the local population. Importantly, almost all parameters were highly correlated, precluding the possibility of clustering the local terminals into non-overlapping subsets. Factor and cluster analysis confirmed these findings. Our results suggest that similarly to excitatory terminals, inhibitory terminals follow an "ultrastructural size principle," and that the terminals of different interneuron subtypes cannot be distinguished by ultrastructure alone.
Assuntos
Hipocampo/fisiologia , Hipocampo/ultraestrutura , Terminações Pré-Sinápticas/fisiologia , Terminações Pré-Sinápticas/ultraestrutura , Ácido gama-Aminobutírico/fisiologia , Animais , Biotina/análogos & derivados , Dextranos , Corantes Fluorescentes , Processamento de Imagem Assistida por Computador , Imuno-Histoquímica , Masculino , Fibras Nervosas/fisiologia , Fibras Nervosas/ultraestrutura , Ratos , Ratos WistarRESUMO
The anatomical connection of the magnocellular isthmic nucleus with the optic tectum was investigated with the axonal tracer biotinylated dextran amine. Following iontophoretic injection of this tracer into different areas of the chick optic tectum, neurones of both magno- and parvocellular isthmic nuclei were labelled together in a topographical arrangement. The number of labelled neurones in the parvocellular nucleus was generally higher than in magnocellular. Using different locations of the tracer injections, systematic shifts in the location of the labelled neurones were detected. The labelled axons were seen to course along the shortest possible distance between the injection site and the cells of origin, i.e., the ventral part of the tectum received projections from neurones located ventrally in the isthmic nuclei, the dorsal tectum from neurones in the dorsal part, and the lateral extension of the tectum from neurones lying midway along the nuclei. This parallel and topographic projection of the two nuclei was primarily observed in sagittal sections. After tracer injections into the magnocellular nucleus, the terminal arbours were seen to extend from the deep layers (11-12) to layer 2 of the tectum. The projections observed appeared to be topographically organised, and furthermore appeared to be parallel with and complimentary to previously described projections of the parvocellular isthmic nucleus.
Assuntos
Galinhas/crescimento & desenvolvimento , Vias Eferentes/anatomia & histologia , Neurônios/citologia , Colículos Superiores/citologia , Animais , Animais Recém-Nascidos , Biotina/análogos & derivados , Biotina/metabolismo , Contagem de Células , Dextranos/metabolismo , Vias Eferentes/metabolismo , Neurônios/metabolismo , Colículos Superiores/crescimento & desenvolvimento , Colículos Superiores/metabolismoRESUMO
The optic tectum is reciprocally connected to the nuclei isthmi pars magnocellularis (Imc) and pars parvocellularis (Ipc), which have different modulatory effects on optic transmission. We studied the axon arbourisation of these isthmic nuclei in the optic tectum in order to differentiate between them using Golgi-impregnated preparations both in chickens and pigeons. In addition, sections from animals injected with the anterograde tracer biotinylated dextran-amine (BDA) into the Imc were examined in the bright-field and electron microscope to identify the axon arbourisations and terminals. Also, GABA immunogold stained sections were examined in the electron microscope. In Golgi preparations, slab-like (or poplar tree-like) axon terminal arbourisations of both magnocellular and parvocellular isthmic nuclei neurons were found extending to the tectal surface, with similar branching patterns, but different lengths. The axon arbourisations extending from layer 5 of the optic tectum to the surface were termed type 1, whereas those extending from the internal (12-11) layers to the tectal surface were termed type 2. Type 2 arbourisations very closely matched arbourisations observed in BDA injected material, indicating that Imc neurons gave rise to type 2 arbourisations. The two kinds of axon arbourisation in the external tectal layers were alike in both types of bird, except for the width, which was about 10 mum larger in the type 2 axon arbour. Controlling for size, there was no significant difference between chicks and pigeons. The significance of these afferents in the optic tectum is discussed.
Assuntos
Axônios/ultraestrutura , Galinhas/anatomia & histologia , Columbidae/anatomia & histologia , Neurônios/ultraestrutura , Colículos Superiores/ultraestrutura , Vias Visuais/ultraestrutura , Animais , Axônios/fisiologia , Biotina/análogos & derivados , Forma Celular/fisiologia , Tamanho Celular , Galinhas/fisiologia , Columbidae/fisiologia , Dextranos , Imuno-Histoquímica , Neurônios/fisiologia , Terminações Pré-Sinápticas/fisiologia , Terminações Pré-Sinápticas/ultraestrutura , Coloração pela Prata , Colículos Superiores/fisiologia , Vias Visuais/fisiologia , Ácido gama-Aminobutírico/metabolismoRESUMO
Recommendation to reduce fat consumption from ruminant meat does not consider the contribution of nutritionally beneficial fatty acids in lean beef. Here we report effects of production system (organic vs conventional) and finishing season on meat and fat quality of sirloin steaks from retail outlets and simulated fatty acid intakes by consumers. There was little difference in meat quality (pH, shear force and colour), but the fat profiles varied considerably between production systems and season. Meat fat from organic and summer finished cattle contained higher concentrations of conjugated linoleic acid, its precursor vaccenic acid and individual omega-3 fatty acids and had a lower ratio of omega-6 to omega-3 fatty acids compared with non-organic and winter finished cattle respectively. The fat profile from summer finished organic beef aligns better to recommended dietary guideline including those for long chain omega-3 fatty acids compared with that from winter finished, non-organic steak.
Assuntos
Ração Animal , Criação de Animais Domésticos/métodos , Composição Corporal , Ácidos Graxos Ômega-3/análise , Alimentos Orgânicos/análise , Carne/análise , Estações do Ano , Animais , Bovinos , Ácidos Graxos Ômega-6/análise , Saúde , Humanos , Carne/normasRESUMO
This study investigated the effect of, and interactions between, contrasting crossbreed genetics (US Brown Swiss [BS] × Improved Braunvieh [BV] × Original Braunvieh [OB]) and feeding regimes (especially grazing intake and pasture type) on milk fatty acid (FA) profiles. Concentrations of total polyunsaturated FAs, total omega-3 FAs and trans palmitoleic, vaccenic, α-linolenic, eicosapentaenoic and docosapentaenoic acids were higher in cows with a low proportion of BS genetics. Highest concentrations of the nutritionally desirable FAs, trans palmitoleic, vaccenic and eicosapentaenoic acids were found for cows with a low proportion of BS genetics (0-24% and/or 25-49%) on high grazing intake (75-100% of dry matter intake) diets. Multivariate analysis indicated that the proportion of OB genetics is a positive driver for nutritionally desirable monounsaturated and polyunsaturated FAs while BS genetics proportion was positive driver for total and undesirable individual saturated FAs. Significant genetics × feeding regime interactions were also detected for a range of FAs.
Assuntos
Bovinos/genética , Indústria de Laticínios/métodos , Leite/química , Ração Animal , Animais , Ácidos Graxos/análise , Ácidos Graxos Ômega-3/análise , Feminino , Pradaria , Hibridização GenéticaRESUMO
Many studies show concentrations of nutritionally desirable fatty acids in bovine milk are lower when cows have no access to grazing, leading to seasonal fluctuations in milk quality if cows are housed for part of the year. This study investigated the potential to improve the fatty acid profiles of bovine milk by oilseed supplementation (rolled linseed and rapeseed) during a period of indoor feeding in both organic and conventional production systems. Both linseed and rapeseed increased the concentrations of total monounsaturated fatty acids, vaccenic acid, oleic acid and rumenic acid in milk, but decreased the concentration of the total and certain individual saturated fatty acids. Linseed resulted in greater changes than rapeseed, and also significantly increased the concentrations of α-linolenic acid, total polyunsaturated fatty acids and total omega-3 fatty acids. The response to oilseed supplementation, with respect to increasing concentrations of vaccenic acid and omega-3 fatty acids, appeared more efficient for organic compared with conventional diets.
Assuntos
Dieta/veterinária , Ácidos Graxos/análise , Óleo de Semente do Linho/administração & dosagem , Leite/química , Óleos de Plantas/administração & dosagem , Ração Animal , Fenômenos Fisiológicos da Nutrição Animal , Animais , Bovinos , Suplementos Nutricionais , Ácidos Graxos Monoinsaturados , Feminino , Humanos , Lactação/efeitos dos fármacos , Óleo de Semente do Linho/farmacologia , Leite/normas , Agricultura Orgânica , Projetos Piloto , Óleos de Plantas/farmacologia , Óleo de Brassica napus , Estações do AnoRESUMO
This study investigates the quality of retail milk labelled as Jersey & Guernsey (JG) when compared with milk without breed specifications (NS) and repeatability of differences over seasons and years. 16 different brands of milk (4 Jersey & Guernsey, 12 non specified breed) were sampled over 2 years on 4 occasions. JG milk was associated with both favourable traits for human health, such as the higher total protein, total casein, α-casein, ß-casein, κ-casein and α-tocopherol contents, and unfavourable traits, such as the higher concentrations of saturated fat, C12:0, C14:0 and lower concentrations of monounsaturated fatty acids. In summer, JG milk had a higher omega-3:omega-6 ratio than had NS milk. Also, the relative increase in omega-3 fatty acids and α-tocopherol, from winter to summer, was greater in JG milk. The latter characteristic could be of use in breeding schemes and farming systems producing niche dairy products. Seasonality had a more marked impact on the fatty acid composition of JG milk than had NS milk, while the opposite was found for protein composition. Potential implication for the findings in human health, producers, industry and consumers are considered.
Assuntos
Bovinos/metabolismo , Leite/química , Animais , Bovinos/classificação , Ácidos Graxos Ômega-3/análise , Ácidos Graxos Ômega-3/metabolismo , Feminino , Humanos , Leite/metabolismo , Proteínas do Leite/análise , Proteínas do Leite/metabolismo , Valor Nutritivo , Controle de Qualidade , Estações do Ano , Reino UnidoRESUMO
The nucleus lentiformis mesencephali magnocellularis (nLMmc) is an essential part of the accessory optic nuclei and is responsible for stabilization of the horizontal eye movement. The morphology of this nucleus and its intrinsic structural connectivity were studied with Golgi, biotinylated dextran amine anterograde immunotracer and GABA immunostaining methods by light and electron microscopy. In the Golgi preparations neurons of large, medium-large, medium and small sizes were distinguished. The small neurons are GABA-immunopositive local circuit neurons, the others are proposed to be partly projection, partly local circuit neurons. The large and medium-large projection neurons are located in a tight topographical relationship observed in the Golgi preparations. The dendrites of the large and medium-large cells are also observed to be in close proximity with each other, and also with retinal fibre terminals. The morphological arrangement suggests that the retinal fibres make synaptic contacts with dendrites from both types of cell, and this is confirmed by the examination of retinal fibre terminals using electron microscopy. The optic fibre terminals establish synaptic contacts with small dendritic branches, dendritic processes and dendritic spines of large and medium-large neurons in the nLMmc. This arrangement allows the two types of nLMmc neuron access to very similar, if not identical, inputs, which may facilitate some of the different aspects of visual processing. Optic transmission by these cells may be modulated by the GABA-immunopositive terminals from various local circuit neurons, and very probably from GABAergic myelinated fibres as well, which may originate from the contralateral nLMmc and/or the visual Wulst.
Assuntos
Corpo Estriado/citologia , Microscopia Imunoeletrônica/métodos , Neurônios/citologia , Animais , Galinhas , Corpo Estriado/metabolismo , Corpo Estriado/ultraestrutura , Dendritos/ultraestrutura , Dextranos/química , Complexo de Golgi/metabolismo , Complexo de Golgi/ultraestrutura , Neurônios/metabolismo , Neurônios/ultraestrutura , Ácido gama-Aminobutírico/metabolismoRESUMO
In the present study the terminals of retinal fibres and those of internal layer cells in ventral geniculate nucleus of chicks were labelled with the anterograde tracer biotinylated dextran amine. The tracer showed the connections from the internal cell layers of ventral geniculate nucleus to the medial part of the dorsal lateral geniculate nucleus. The labelled retinal terminals were located exactly in the lateral part of nucleus. The labelled terminals in the two parts of the nucleus were analysed with the electron microscope and showed a different synaptic organisation in the two parts of the dorsal lateral geniculate nucleus. In the lateral part, two kinds of synaptic glomeruli were found mostly in the vicinity of large dendrites, which are proximal dendrites of projection neurons. One type is a simple glomerulus containing a large dendrite, a large optic terminal and a large and/or series of asymmetrical synapses surrounded by glial processes. The other type is a complex synaptic unit with several pre- and postsynaptic components, among them synapses of GABA-positive axon terminals and/or dendraxons. No glomeruli were found in the medial part of the nucleus. In the medial part of the lateral geniculate nucleus, the terminals of internal layer cell axons established asymmetrical synapses with dendrites. Often, a large terminals and large dendritic profiles established serial asymmetrical synapses. GABA-positive myelinated fibres entered and ramified in both parts of the dorsal lateral geniculate nucleus, and GABA-positive terminals were seen to form synapses on the same dendrite near to the asymmetrical contacts. To our knowledge, this is the first report of the connection from ventral geniculate internal layer cells to the dorsal lateral geniculate nucleus in the chick.
Assuntos
Corpos Geniculados/anatomia & histologia , Corpos Geniculados/metabolismo , Sinapses/fisiologia , Animais , Encéfalo/anatomia & histologia , Encéfalo/metabolismo , Núcleo Celular/metabolismo , Tamanho Celular , Galinhas , Dendritos/metabolismo , Dextranos/farmacologia , Complexo de Golgi/metabolismo , Imuno-Histoquímica , Microscopia Eletrônica , Terminações Nervosas/anatomia & histologia , Neurônios/metabolismo , Fatores de Tempo , Ácido gama-Aminobutírico/metabolismoRESUMO
Predictions of plant and animal species distributions are important for conservation and for the assessment of large-scale ecosystem change. Land cover data are becoming more widely available for use in land management and conservation. We use a logistic regression modelling approach to investigate the utility of these data for modelling. The relationship between the distribution of 137 British ground beetles species and land cover was investigated using data from 1,687 10 km national grid squares. Land cover data were simplified using ordination and the axes used as predictors in logistic regression with presence absence data for individual beetle species as response variables. Significant regression models were generated for all species with first and second axis scores. The amounts of variation explained by models were generally low, but predictions derived from models generally matched the known distributions of the species in Britain. Species with coastal preferences were poorly modelled and predicted to occur throughout lowland Britain whilst a number of species occurring in southern Britain were predicted to occur into Scotland. A validation exercise comparing model predictions with new data from a survey of 59 10 km(2) produced mixed results with the distribution of grassland species being better predicted than riverine species. Jack-knifing was used to assess the robustness of models for four species which differed in their apparent responses to the land cover variables. Methods for improving the predictive power of these models and their potential for use in assessing the impact of global climate change are discussed.