Human gnathostomiasis caused by Gnathostoma doloresi, with particular reference to the parasitological investigation of the causative agent.

Gnathostomiasis caused by Gnathostoma doloresi Tubangui was observed in a 46-year-old Japanese man, a clerk, who resided in the Fukuoka prefecture of Kyushu district, in southern Japan. The first symptom noted by the patient was epigastric pain, which occurred 3 days after he had consumed the raw flesh of a brook trout, Oncorhynchus masou masou (Brevoort), from a trout farm in neighbouring Kumamoto prefecture. Three days later, he developed creeping eruptions on his trunk. The eruptions spread and the patient was admitted to hospital 18 days after eating the trout. No nematode larvae were found in any of four skin biopsies collected on admission. However, vesicles appeared on the lower abdomen of the patient 20 days after he had eaten the trout, and a small nematode was removed from one of them. One day after the nematode had been excised, a cutaneous swelling suddenly occurred on the patient's lower left jowl and this persisted for a week. All the skin abnormalities gradually decreased from day 25 post-ingestion and had disappeared by day 30. The isolated nematode was identified as an advanced third-stage larva of G. doloresi, based on its morphology and the number of hooklets on its head-bulb.

Characterization of transplant generations of primary rat adenocarcinoma and some chemotherapeutic observations.

Rats (Wistar) bearing subcutaneous implants of tumor fragment of DMBA-induced tumor were used in this study. Studies have been made of their proliferative characteristics, and their response to Cis-dichlorodiammine platinum (CDDP) has been measured. The transplanted tumor appears to be on homogeneous solid structure throughout all transplant generations, although the primary site is concomitant adenocarcinoma showing both glandular and solid structure. Microangiography showed that the transplanted tumor was more hypervascular than the primary tumor. Compared with the previous reports using the primary autochthonous tumor, the antitumor effect of CDDP is excellent and the histologic effect is observed in all areas of the sectioned surface. The transplanted tumor could provide a useful experimental system for the laboratory study of the chemotherapy of ovarian adenocarcinoma and the possibility of predicting ovarian adenocarcinoma seemed to be great because of the morphological similarity.