RESUMO
The lack of vitamin B12 in unprocessed plant-based foods can lead to health problems in strict vegetarians and vegans. The main aim of this study was to investigate the potential synergy of co-culturing Bifidobacterium animalis subsp. lactis and Propionibacterium freudenreichii in improving production of vitamin B12 and short-chain fatty acids in soy whey. Different strategies including mono-, sequential and simultaneous cultures were adopted. Growth, short-chain fatty acids and vitamin B12 were assessed throughout the fermentation while free amino acids, volatiles, and isoflavones were determined on the final day. P. freudenreichii monoculture grew well in soy whey, whereas B. lactis monoculture entered the death phase by day 4. Principal component analysis demonstrates that metabolic changes in both sequential cultures did not show drastic differences to those of P. freudenreichii monoculture. However, simultaneous culturing significantly improved vitamin B12, acetic acid and propionic acid contents (1.3 times, 5 times, 2.5 times, compared to the next highest treatment [sequential cultures]) in fermented soy whey relative to other culturing modes. Hence, co-culturing of P. freudenreichii and B. lactis would provide an alternative method to improve vitamin B12, acetic acid and propionic acid contents in fermented foods.
Assuntos
Bifidobacterium animalis , Propionibacterium freudenreichii , Propionatos , Propionibacterium freudenreichii/metabolismo , Bifidobacterium animalis/metabolismo , Soro do Leite , Vitamina B 12/análise , Vitamina B 12/metabolismo , Propionibacterium/metabolismo , Ácidos Graxos Voláteis/metabolismo , Fermentação , Ácido Acético/metabolismo , Proteínas do Soro do Leite/metabolismo , Vitaminas/metabolismoRESUMO
Drought events are a major challenge for many horticultural crops, including grapes, which are often cultivated in dry and warm climates. It is not understood how the cuticle contributes to the grape berry response to water deficit (WD); furthermore, the cuticular waxes and the related biosynthetic pathways are poorly characterized in this fruit. In this study, we identified candidate wax-related genes from the grapevine genome by phylogenetic and transcriptomic analyses. Developmental and stress response expression patterns of these candidates were characterized across pre-existing RNA sequencing data sets and confirmed a high responsiveness of the pathway to environmental stresses. We then characterized the developmental and WD-induced changes in berry cuticular wax composition, and quantified differences in berry transpiration. Cuticular aliphatic wax content was modulated during development and an increase was observed under WD, with wax esters being strongly up-regulated. These compositional changes were related to up-regulated candidate genes of the aliphatic wax biosynthetic pathway, including CER10, CER2, CER3, CER1, CER4, and WSD1. The effect of WD on berry transpiration was not significant. This study indicates that changes in cuticular wax amount and composition are part of the metabolic response of the grape berry to WD, but these changes do not reduce berry transpiration.
Assuntos
Vitis , Secas , Frutas/genética , Filogenia , Vitis/genética , CerasRESUMO
Soy whey, a by-product from the tofu and soy protein isolate industry was evaluated as a substrate for a biofortified beverage using several propionic acid bacteria (PAB). PAB growth and changes in sugars, organic acids, amino acids and isoflavones were investigated. Vitamin B12 and short-chain fatty acid (SCFA) production were measured over time. Acidipropionibacterium acidipropionici (DSM 20272) showed the highest growth, compared to the other three PABs (Propionibacterium freudenreichii [DSM 20271 and DSM 4902], A. jensenii [DSM 20535]). Acidipropionibacterium (DSM 20272 and DSM 20535) showed the best propionic acid and acetic acid production, while P. freudenreichii produced the most succinic acid. Propionibacterium freudenreichii exhibited significant vitamin B12 production at 4.06 ± 0.28 µg/L for DSM 20271, followed by 2.58 ± 0.22 µg/L for DSM 4902. Notably, all PAB displayed strong ß-glycosidase activities evidenced by the conversion of isoflavone glycosides to isoflavone aglycones. The stark differences between Acidipropionibacterium spp. and Propionibacterium spp. indicate that the former PAB is specialized in SCFA production, while the latter PAB is better at vitamin B12 bioenrichment. This study demonstrated the possibility of employing PAB fermentation to improve SCFA and vitamin B12 content. This can open avenues for a beverage or functional ingredient development.
Assuntos
Isoflavonas , Alimentos de Soja , Propionatos/metabolismo , Soro do Leite/metabolismo , Propionibacterium/metabolismo , Proteínas do Soro do Leite/metabolismo , Fermentação , Bactérias/metabolismo , Biotransformação , Isoflavonas/metabolismo , Vitaminas/metabolismoRESUMO
Terpenes play a formative role in grape and wine flavor, particularly for high-terpenic cultivars. Differences in terpene profiles influence grape varietal character and vintage quality. Little is known about the endogenous factors controlling terpene biosynthesis in grape. Through multiple experiments, six hormones (abscisic acid, ABA; ethylene, ETH; jasmonic acid, JA; methyl jasmonate, MeJA; indole-3-acetic acid, IAA; 1-naphthaleneacetic acid, NAA) that either promote or repress ripening were applied to Gewürztraminer clusters near veraison to gauge their effect on ripening and terpene biosynthesis. Jasmonates (JA, MeJA) increased terpene concentrations and the expression of terpene genes in grapes. Such increases were not associated to increases of other ripening-related metabolites such as sugars or anthocyanins. MeJA also affected the expression of several hormone related genes, increased IAA levels, and reduced sugar and anthocyanin concentration in grapes. This research provides novel insights into terpene regulation by ripening-related hormones and jasmonates in grapes.
Assuntos
Vitis , Antocianinas/metabolismo , Ciclopentanos , Frutas/genética , Frutas/metabolismo , Regulação da Expressão Gênica de Plantas , Hormônios , Oxilipinas , Terpenos/metabolismo , Vitis/genética , Vitis/metabolismoRESUMO
Regulated deficit irrigation (RDI) is a viticultural practice known to improve grape phenolics and color in red grapes; however, the impact of this practice on grape aromas remains largely unknown. The effects of RDI treatments applied at various berry developmental stages on canopy, yield, and free and glycoside-bound terpenes of the berry were investigated for three consecutive seasons. All RDI treatments reduced leaf photosynthesis and yield, but not when applied after veraison. Berry total soluble solids at harvest were reduced by RDI applied after veraison or throughout the season. Despite reducing berry sugars, RDI applied after veraison increased the concentration at harvest of key free terpenes for Gewürztraminer grapes such as geraniol and citronellol. RDI treatments down-regulated some terpene genes, which indicates that the observed changes in terpene concentration were not transcriptionally regulated. This study suggests that RDI applied after version can potentially improve wine aroma in Gewürztraminer.
Assuntos
Irrigação Agrícola/métodos , Frutas/metabolismo , Terpenos/metabolismo , Vitis/crescimento & desenvolvimento , Vitis/metabolismo , Colúmbia Britânica , Frutas/genética , Frutas/crescimento & desenvolvimento , Regulação da Expressão Gênica de Plantas , Odorantes/análise , Fenóis , Fotossíntese , Folhas de Planta/fisiologia , Estações do Ano , Vitis/genética , Compostos Orgânicos Voláteis/análiseRESUMO
Recent studies revealed that terpenes present in grapes determine the 'stone fruit' aroma of Viognier wines. This study analysed the evolution of free and glycosylated terpenes along with other volatile organic compounds (VOCs) during berry development in Viognier berries exposed to three irrigation regimes. These regimes (standard irrigation over the season, early deficit irrigation from 25â¯days after anthesis to veraison, prolonged deficit irrigation from 25â¯days after anthesis to harvest) were imposed to Viognier grapevines grown in the Okanagan Valley (British Columbia, Canada). Berry samples were collected over the season and analysed for determining the chemical composition, including free and glycosylated VOCs, and the expression of genes related to VOC biosynthesis. Among free VOCs, aldehydes peaked at veraison, alcohols did not vary across berry development, and terpenes increased during ripening. Among glycosylated VOCs, aldehydes and terpenes mirrored the free fractions while alcohols peaked before veraison. At harvest, a larger concentration of linalool oxides was detected in the glycosylated than in the free fraction. Increases of terpenes during ripening were modulated at the transcriptional level, particularly by two terpene synthases VviCSbOci and VviPNLinNer1. Early deficit irrigation increased the concentration of free α-terpineol and linalool, while prolonged deficit irrigation reduced the concentration of glycosylated α-terpineol. These results provide a basis for further studies on managing Viognier grape aroma in vineyards.