RESUMO
OBJECTIVE: To evaluate the relationship of hip radiographic osteoarthritis (ROA) and MRI findings of cartilage lesions, labral tears, bone marrow edema-like lesions (BMELs) and subchondral cysts with self-reported and physical function. DESIGN: Eighty five subjects were classified as controls (n = 55, Kellgren-Lawrence (KL) 0, 1) or having mild-moderate ROA (n = 30, KL 2, 3). T2 weighted MRI images at 3-T were graded for presence of cartilage lesions, labral tears, BMELs and subchondral cysts. Posterior wall sign, cross-over sign, center-edge angle and alpha angle were also recorded. Function was assessed using Hip dysfunction and Osteoarthritis Outcome Score (HOOS), Timed-Up and Go (TUG) test and Y-Balance Test (YBT). Analysis compared function between subjects with and without ROA and those with and without femoral or acetabular cartilage lesions, adjusted for age. Non-parametric correlations were used to assess the relationship between radiographic scores, MRI scores and function. RESULTS: Subjects with acetabular cartilage lesions had worse HOOS (Difference = 5-10%, P = 0.036-0.004), but not TUG or YBT, scores. Acetabular cartilage lesions, BMELs and subchondral cysts were associated with worse HOOS scores (ρ = 0.23-0.37, P = 0.041-0.001). Differences in function between subjects with and without ROA or femoral cartilage lesions were not significant. Other radiologic findings were not associated with function. CONCLUSIONS: Acetabular cartilage defects, but not femoral cartilage defects or ROA, were associated with greater self-reported pain and disability. BMELs and subchondral cysts were related to greater hip related self-reported pain and disability. None of the radiographic or MRI features was related to physical function.
Assuntos
Cartilagem Articular/lesões , Osteoartrite do Quadril/complicações , Dor/etiologia , Adulto , Idoso , Cistos Ósseos/diagnóstico , Cistos Ósseos/etiologia , Doenças da Medula Óssea/diagnóstico , Doenças da Medula Óssea/etiologia , Cartilagem Articular/patologia , Estudos de Casos e Controles , Teste de Esforço/métodos , Feminino , Humanos , Imageamento por Ressonância Magnética/métodos , Masculino , Pessoa de Meia-Idade , Osteoartrite do Quadril/diagnóstico por imagem , Osteoartrite do Quadril/fisiopatologia , Medição da Dor/métodos , Radiografia , Autorrelato , Índice de Gravidade de DoençaRESUMO
Weaned pigs from a line bred for increased feed efficiency were enrolled in a study of the role of host genes in the response to infection with Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). Four of the pigs were euthanatized early in the study due to weight loss with illness and poor body condition; 2 pigs before PRRSV infection and the other 2 pigs approximately 2 weeks after virus inoculation. The 2 inoculated pigs failed to produce PRRSV-specific antibodies. Gross findings included pneumonia, absence of a detectable thymus, and small secondary lymphoid tissues. Histologically, lymph nodes, spleen, tonsils, and Peyer's patches were sparsely cellular with decreased to absent T and B lymphocytes.
Assuntos
Síndromes de Imunodeficiência/veterinária , Tecido Linfoide/patologia , Síndrome Respiratória e Reprodutiva Suína/imunologia , Vírus da Síndrome Respiratória e Reprodutiva Suína/isolamento & purificação , Animais , Anticorpos Antivirais/biossíntese , Anticorpos Antivirais/imunologia , Linfócitos B/imunologia , Linfócitos B/virologia , Diagnóstico Diferencial , Síndromes de Imunodeficiência/patologia , Síndromes de Imunodeficiência/virologia , Pulmão/patologia , Pulmão/virologia , Linfonodos/imunologia , Linfonodos/patologia , Linfonodos/virologia , Tecido Linfoide/imunologia , Tecido Linfoide/virologia , Masculino , Tonsila Palatina/imunologia , Tonsila Palatina/patologia , Tonsila Palatina/virologia , Nódulos Linfáticos Agregados/imunologia , Nódulos Linfáticos Agregados/patologia , Nódulos Linfáticos Agregados/virologia , Pneumonia/veterinária , Pneumonia/virologia , Síndrome Respiratória e Reprodutiva Suína/patologia , Síndrome Respiratória e Reprodutiva Suína/virologia , Vírus da Síndrome Respiratória e Reprodutiva Suína/genética , Vírus da Síndrome Respiratória e Reprodutiva Suína/imunologia , Baço/imunologia , Baço/patologia , Baço/virologia , Suínos , Linfócitos T/imunologia , Linfócitos T/virologia , Viremia/veterináriaRESUMO
Alveolar macrophages from PRRSV-infected and naïve pigs were placed into culture and infected with PRRSV laboratory strain SD-23983. Permissiveness increased with time in culture, and macrophages from infected pigs could be superinfected. Addition of actinomycin D, an inhibitor of mRNA synthesis, blocked infection. Interferon-gamma reduced infection in cultures, while the addition of tumor necrosis factor-alpha or interleukin (IL)-10 did not affect permissiveness. IL-4 produced a marginal increase in the percentage of infected cells, but without a detectable increase in virus yield. These results suggest that the PRRSV-permissive population of cells in culture arises from a non-permissive precursor population and depends on new mRNA synthesis.
Assuntos
Macrófagos Alveolares/virologia , Síndrome Respiratória e Reprodutiva Suína/virologia , Vírus da Síndrome Respiratória e Reprodutiva Suína/fisiologia , Animais , Antivirais/farmacologia , Células Cultivadas , Dactinomicina/farmacologia , Interferon gama/farmacologia , Interleucina-10/farmacologia , Macrófagos Alveolares/citologia , Inibidores da Síntese de Ácido Nucleico/farmacologia , Síndrome Respiratória e Reprodutiva Suína/imunologia , Vírus da Síndrome Respiratória e Reprodutiva Suína/efeitos dos fármacos , RNA Mensageiro/metabolismo , Sus scrofa , Fatores de TempoRESUMO
We had previously demonstrated that a Type-1-like immune response involving interferon-gamma expression in lamina propria lymphocytes accompanied by IgG2 subclass fecal antibodies to Cryptosporidium parvum p23 emerged in gut mucosa of calves recovering from cryptosporidiosis. Because a recombinant p23 had been shown to protect calves from cryptosporidiosis when administered as a vaccine antigen to late gestation cattle, this study was undertaken to determine if the same vaccine antigen could induce a Type-1-like, in vitro response by T cells from calves that had recovered from C. parvum infection. We isolated peripheral blood mononuclear cells from calves that had been previously infected with C. parvum oocysts and incubated them in the presence or absence of the recombinant C. parvum p23 vaccine antigen. We used flow cytometry to simultaneously detect cells in cell cycle and identify the T cell subset containing cycling cells. We also used flow cytometry to identify interferon-gamma positive cells and 2-dimensional gel electrophoresis to profile proteins made by PBMC stimulated with the recombinant p23 vaccine antigen. The results demonstrated that CD4+ T lymphocytes proliferated and that interferon-gamma was synthesized by a subset of stimulated cells. Two-dimensional gel electrophoresis revealed the presence of several cytoplasmic proteins in a size range of approximately 25-80 kDa that were detected in p23-stimulated, but not in unstimulated, cytoplasmic samples. Together, the results show that the recombinant p23 vaccine antigen can stimulate a Type-1-like immune response by T cells from calves that have recovered from C. parvum infection.
Assuntos
Antígenos de Protozoários/imunologia , Doenças dos Bovinos/imunologia , Criptosporidiose/veterinária , Cryptosporidium parvum/imunologia , Vacinas Protozoárias/imunologia , Linfócitos T/imunologia , Animais , Bovinos , Doenças dos Bovinos/prevenção & controle , Proliferação de Células , Criptosporidiose/imunologia , Criptosporidiose/prevenção & controle , Eletroforese em Gel Bidimensional/veterinária , Citometria de Fluxo/veterinária , Imunofenotipagem/veterinária , Interferon gama/biossíntese , Vacinas Sintéticas/imunologiaRESUMO
In the study reported here, we used RT-PCR with primers specific for interleukin-1 (IL-1), IL-6, IL-10, tumor necrosis factor-alpha (TNF-alpha), and nitric oxide synthase (iNOS) to assess the cytokine mRNA expression associated with bovine blood monocytes during their differentiation to macrophages cultured on plastic (1 week). In addition, we used RT-PCR to assess the contribution of gammadelta T cells as a source of interferon-gamma (IFN-gamma), the induction signal for iNOS. Further, we evaluated cytocentrifuge preparations from the cultures for the production of IL-10 using specific antibody. We previously demonstrated that iNOS can be induced in cultured bovine monocytes in response to IFN-gamma and TNF-alpha but lose this capability in a short period of time. However, we demonstrate here that iNOS induction from monocytes cultured with IFN-gamma secreting gammadelta T cells is prolonged, suggesting that this source of IFN-gamma primes the monocytes before exogenous stimulation. Based on mRNA expression, placement of monocytes in culture resulted in activation, followed by quiescence. By 6 days in culture, the iNOS message was reduced below the basal level. In addition, the TNF-alpha message was substantially reduced, and IL-1 and IL-6 messages were reduced below detectable levels. This correlated with an increase in IL-10 message. Downregulation of these same cytokine messages as well as IFN-gamma message occurred within a 20-h period when IL-10 was added exogenously to cultures of total leukocytes. At the same time, there was an increase in the number of IL-10-positive cells and an increase in the intensity of anti-IL-10 staining within adherent cells. These results provide evidence for IL-10 regulation of some bovine mononuclear phagocyte effector functions.
Assuntos
Citocinas/genética , Interleucina-10/fisiologia , Leucócitos Mononucleares/metabolismo , Macrófagos/metabolismo , Óxido Nítrico Sintase/genética , RNA Mensageiro/biossíntese , Animais , Bovinos , Adesão Celular , Diferenciação Celular/fisiologia , Regulação para Baixo , Indução Enzimática , Interferon gama/biossíntese , Leucócitos Mononucleares/citologia , Macrófagos/citologia , Óxido Nítrico/biossíntese , Óxido Nítrico Sintase Tipo II , Reação em Cadeia da Polimerase/métodos , Transcrição GênicaRESUMO
We have developed a rapid, reliable method of evaluating growth and viability of intraerythrocytic protozoan hemoparasites. The assay involves the selective uptake and metabolic conversion of hydroethidine to ethidium by live parasites present in intact erythrocytes. The red fluorescence imparted by ethidium intercalated into the DNA of the parasite permits the use of flow cytometry to distinguish infected erythrocytes with viable parasites from uninfected erythrocytes and erythrocytes containing dead parasites. Comparison of the fluorochromasia technique of enumerating the number and viability of hemoparasites in cultured erythrocytes with enumeration in Giemsa-stained films and uptake of [3H]hypoxanthine demonstrated the fluorochromasia technique yields comparable results. Studies with the hemoparasite, Babesia bovis, have shown the fluorochromasia technique can also be used to monitor the effect of parasiticidal drugs on parasites in vitro. The cumulative studies with the fluorochromasia assay suggest the assay will also prove useful in investigations focused on analysis of the immune response to hemoparasites and growth in vitro.
Assuntos
Babesia/fisiologia , Eritrócitos/parasitologia , Citometria de Fluxo , Animais , Corantes Azur , Divisão Celular , Sobrevivência Celular , Resistência a Medicamentos , Corantes Fluorescentes , FenantridinasRESUMO
A 32-day-old horse with severe combined immunodeficiency was transplanted with equine bone marrow cells in an attempt to establish immunologic responsiveness. A histocompatible, mixed-leukocyte-culture-nonreactive, sex-matched, full sibling was used as the donor. Recipient total lymphocyte count, T and B lymphocyte numbers, and response of peripheral blood mononuclear cells to phytolectin stimulation increased by 14 days following transplantation. Circulating lymphocytes exceeded 1000 cells/microliter blood by 40 days posttransplantation, and by 170 days following transplantation, T and B lymphocyte numbers had reached normal values. The foal demonstrated significant primary and secondary antibody responses when immunized with bacteriophage phi X 174 at 100 and 142 days posttransplantation. Concentrations of IgG and IgM remained within the normal range following cessation of i.v. plasma therapy 156 days after transplantation. More than 300 days following transplantation, the foal remains healthy and is growing normally. At no time during the posttransplant period was there detectable evidence of graft-versus-host disease.
Assuntos
Transplante de Medula Óssea , Síndromes de Imunodeficiência/terapia , Animais , Feminino , Cavalos , Masculino , Transplante de Células-Tronco , Fatores de TempoRESUMO
Secreted proteins from cultured rat Sertoli cells were assessed for effects on phytolectin-stimulated rat splenic lymphocytes. Sertoli cell proteins (SCP) suppressed DNA, RNA and protein synthesis in stimulated rat splenic lymphocytes whether added at 0, 4, 24 and 48 h after culture initiation. SCP preparations were not toxic to cells. SCP suppressive activity was heat stable but was not associated with the carbohydrate component of SCP preparations. SCP also suppressed the proliferation of lymphoid and non-lymphoid cell lines from several different animal species but did not inhibit proliferation-independent lysis of YAC-1 target cells by rat natural killer cells. These results suggest that Sertoli cells synthesize inhibitory factors that might be secreted into seminal plasma. Furthermore, our results demonstrate that one mode of action of these factors is suppression of cell proliferation.
Assuntos
Ativação Linfocitária , Proteínas/imunologia , Células de Sertoli/imunologia , Fatores Supressores Imunológicos/metabolismo , Animais , Células Cultivadas , Células Matadoras Naturais/imunologia , Lectinas/farmacologia , Masculino , Proteínas/metabolismo , Ratos , Sêmen/imunologia , Células de Sertoli/metabolismoRESUMO
We have developed an assay to detect mucosally delivered antibody to Cryptosporidium parvum sporozoite antigens. We absorbed a recombinant 23-kD sporozoite protein to polystyrene microspheres, and used flow cytometry to detect, titer, and determine the isotype of antibody to p23 that was shed in the feces of experimentally infected calves. Noninoculated calves have low levels of mucosal antibody to p23, with IgG1 as the predominant isotype. Antibody titers rise in inoculated calves as the animals recover from cryptosporidiosis. A calf that was naturally protected from cryptosporidiosis had mucosal IgM and IgG1 isotype anti-p23 antibodies prior to challenge with C. parvum oocysts. Ten days after challenge, the calf had high titers of IgM, IgA, IgG1, and IgG2 anti-p23 antibodies. Together, the data show that this method can be used to assess mucosally delivered antibody to C. parvum.
Assuntos
Anticorpos Antiprotozoários/análise , Doenças dos Bovinos/imunologia , Criptosporidiose/veterinária , Cryptosporidium parvum/imunologia , Imunidade nas Mucosas , Animais , Antígenos de Protozoários/imunologia , Bovinos , Criptosporidiose/imunologia , Cryptosporidium parvum/isolamento & purificação , Fezes/parasitologia , Isotipos de Imunoglobulinas/análise , Microesferas , Proteínas Recombinantes/imunologiaRESUMO
Products released from activated macrophages have been demonstrated to have microbicidal activity against a variety of microorganisms. Reactive oxygen intermediates (ROI) and reactive nitrogen intermediates (RNI) have been shown to affect the induction of degenerate (crisis) forms of Plasmodium spp. Polyamines are degraded into acrolein which has also been shown to be toxic to Plasmodium spp. We have investigated the possibility that these products act similarly with Babesia bovis. Crisis forms of B. bovis developed in erythrocyte cultures after the introduction of supernatants containing ROI, RNI, and acrolein. Xanthine degradation by xanthine oxidase leads to the formation of superoxide anion, hydrogen peroxide, and hydroxyl radicals. The degradation in the presence of B. bovis was toxic to the parasite. The toxicity was partially reversed by the addition of the ROI scavenger catalase. However, H2O2 added directly had little effect, suggesting a role for the other ROI products. Spermine degradation by polyamine oxidase and direct addition of acrolein was toxic in a dose-dependent manner. Finally, spontaneous generation of nitric oxide from sodium nitroprusside or S-nitroso-N-acetyl-penicillamine was also toxic in a dose-dependent manner. These data lead us to suggest a role for activated macrophages in the primary immune response against B. bovis.
Assuntos
Antiprotozoários/toxicidade , Babesia bovis/efeitos dos fármacos , Nitroprussiato/toxicidade , Oxidantes/toxicidade , Poliaminas/metabolismo , Poliaminas/toxicidade , Espécies Reativas de Oxigênio/toxicidade , Acroleína/toxicidade , Animais , Babesia bovis/crescimento & desenvolvimento , Babesia bovis/metabolismo , Catalase/farmacologia , Peróxido de Hidrogênio/toxicidade , Hidróxidos/toxicidade , Nitritos/toxicidade , Penicilamina/análogos & derivados , Penicilamina/toxicidade , S-Nitroso-N-Acetilpenicilamina , Espermidina/toxicidade , Espermina/toxicidade , Superóxidos/toxicidade , Xantina , Xantina Oxidase/toxicidade , Xantinas/metabolismoRESUMO
The effect of salivary gland extract of the stable fly, Stomoxys calcitrans (L), on bovine lymphocyte proliferation was determined, and antibody reactivity to salivary gland proteins was characterized in cattle exposed to stable flies. Salivary glands were dissected from male and female flies (4-8 d after eclosion), and protein extracts were made by freeze-thaw cycles. Salivary gland extract (SGE, 1 and 5 microg) significantly inhibited mitogen-driven proliferation of bovine lymphocytes, compared with 1 and 5 microg of identically prepared midgut extract (ANOVA, P < 0.05). Phytohemagglutinin A (PHA) stimulated lymphocyte responses were suppressed by 61.7 and 79.5% (mean values) with 1 and 5 microg of SCE, whereas concanvalin A (Con A) stimulated responses were suppressed by 62.9 and 77.1% (1 and 5 microg). In contrast, midgut extract (1 and 5 microg) minimally suppressed PHA (12.7% +/- 12.6 and 18.7% +/- 15.5) and Con A-driven responses (13.8% +/- 20.5 and 24.6% +/- 14.9), respectively. Viability studies using propidium iodide and flow cytometry demonstrated that SGE was not cytotoxic. Two-color immunofluorescence studies identified T and B lymphocytes as the nonviable cells in the cultures. Western blot analysis of serum collected from five dairy cows during periods of low and high fly exposure identified an immunodominant 27 kDa protein among the salivary gland proteins. These results indicate that exposure of cattle to stable fly saliva during blood feeding results in an antibody response to salivary proteins and that the saliva has a potential to modulate T lymphocyte function.
Assuntos
Ativação Linfocitária/efeitos dos fármacos , Linfócitos/efeitos dos fármacos , Muscidae/fisiologia , Glândulas Salivares/fisiologia , Extratos de Tecidos/farmacologia , Análise de Variância , Animais , Bovinos , Sobrevivência Celular/efeitos dos fármacos , Fenômenos Fisiológicos do Sistema Digestório , Feminino , Linfócitos/citologia , Linfócitos/imunologiaRESUMO
Neoplastic lymphocytes from a horse with lymphosarcoma and IgM deficiency were analyzed for ability to grow in culture; surface and cytoplasmic IgM; functional activity in blastogenesis, cytoxicity, and suppressor assays; and activities of six enzymes involved in purine and pyrimidine metabolism. The cells lacked surface and cytoplasmic IgM. They had elevated activity of adenosine deaminase and reduced activity of purine nucleoside phosphorylase. Neoplastic cells were nonresponsive in blastogenesis assay and did not kill allogeneic lymphocyte target cells or YAC-1 targets in a lectin-dependent cytotoxicity assay, however, the cells were active in a suppressor assay. They were grown for 16 weeks in cultures supplemented with interleukin 2, during which time the cells retained suppressive activity. These results are consistent with a T cell lymphoma of suppressor cell origin, and may explain the deficiency of IgM observed in some horses with lymphoreticular neoplasms.
Assuntos
Disgamaglobulinemia/veterinária , Doenças dos Cavalos/sangue , Imunoglobulina M/deficiência , Linfócitos/fisiologia , Linfoma não Hodgkin/veterinária , Animais , Disgamaglobulinemia/sangue , Disgamaglobulinemia/complicações , Cavalos , Ativação Linfocitária , Linfócitos/enzimologia , Linfoma não Hodgkin/sangue , Linfoma não Hodgkin/complicações , MasculinoRESUMO
Six monoclonal antibodies reacting with equine T lymphocytes at different stages of maturation were selected from antibodies produced against lymphoid cell preparations. EqT12 and EqT13 antibodies identified subsets of cortical thymocytes with high terminal deoxynucleotidyltransferase (TdT) activity and no phytolectin responsiveness. EqT12+ thymocytes were scattered throughout the cortex while EqT13+ thymocytes were located in the subcapsular cortex. EqT12 bound to small numbers of bone marrow cells, splenocytes, and circulating lymphoid cells, but not to mature T lymphocytes. EqT13 bound to very small numbers of bone marrow cells but not to more mature lymphocytes. EqT6 and EqT7 reacted with a large population of cortical thymocytes with high TdT activity and no phytolectin responsiveness. EqT2 and EqT3 bound primarily to medullary thymocytes with low TdT activity. Eq2+ thymocytes responded to phytolectin stimulation while EqT3+ thymocytes did not. EqT2 and EqT3 bound to 33% and 91% of circulating T lymphocytes, respectively. The T lymphocytes bound by both antibodies included cells capable of suppressing a mixed lymphocyte reaction. Thus, EqT12 and EqT13 identify cells with the functional characteristics of prothymocytes. EqT6 and EqT7 identify resident cortical thymocytes, and EqT2 and EqT3 identify a subpopulation of mature T lymphocytes and all mature T lymphocytes, respectively.
Assuntos
Cavalos/imunologia , Linfócitos T/imunologia , Animais , Anticorpos Monoclonais/imunologia , Antígenos de Diferenciação de Linfócitos T/imunologia , Diferenciação Celular , Desenvolvimento Embrionário e Fetal , Técnicas In Vitro , Ativação Linfocitária , Teste de Cultura Mista de Linfócitos , Fito-Hemaglutininas/farmacologia , Linfócitos T/citologiaRESUMO
A monoclonal antibody (mAb), MM1A, that identifies a molecule expressed on a large percentage of bovine lymphocytes (60-80%) was examined to determine its specificity. Two and three colour immunofluorescence analysis using flow cytometry revealed the molecule is highly expressed on all CD4+ and CD8+ lymphocytes and lymphocytes that express WC1 and the gamma/delta TCR. In contrast to mAbs reactive with BoCD5, MM1A did not react with B lymphocytes. Biochemical analysis revealed that the mAb immunoprecipitates a molecular complex comprising a set of peptides with M(r) approximately 12, 16, 22, 32, 36, and 44 kDa under reducing conditions and an additional 96 kDa peptide complex under non-reducing conditions. The data indicate that MM1A recognizes the bovine orthologue of CD3 (BoCD3).
Assuntos
Anticorpos Monoclonais/imunologia , Antígenos CD/imunologia , Bovinos/imunologia , Linfócitos/imunologia , Animais , Anticorpos Monoclonais/isolamento & purificação , Especificidade de Anticorpos , Reações Antígeno-Anticorpo , Reações Cruzadas , Citometria de Fluxo/veterinária , Cabras/imunologia , Ovinos/imunologiaRESUMO
Fecal samples obtained at intervals from six calves with acute cryptosporidiosis contained antibodies of multiple isotypes to p23. IgM-, IgA-, and IgG(1)-isotype anti-p23 appeared before IgG(2)-isotype antibodies. All anti-p23 antibodies had declined by 2 months after infection. One calf that failed to shed oocysts following initial exposure developed IgG(1)-isotype anti-p23 antibodies. One calf that died following exposure to Cryptosporidium parvum oocysts lacked detectable anti-p23 antibodies. Re-inoculation with C. parvum resulted in a brief, marked recall response to p23.
Assuntos
Anticorpos Antiprotozoários/imunologia , Doenças dos Bovinos/imunologia , Criptosporidiose/veterinária , Cryptosporidium parvum/imunologia , Imunidade nas Mucosas/fisiologia , Animais , Animais Recém-Nascidos , Anticorpos Antiprotozoários/fisiologia , Bovinos , Doenças dos Bovinos/parasitologia , Criptosporidiose/imunologia , Criptosporidiose/parasitologia , Cryptosporidium parvum/crescimento & desenvolvimento , Fezes/parasitologia , Citometria de Fluxo/veterinária , Imunoensaio/veterinária , Isotipos de Imunoglobulinas/imunologia , Isotipos de Imunoglobulinas/fisiologia , Masculino , Microesferas , Organismos Livres de Patógenos EspecíficosRESUMO
Ileal intraepithelial and lamina propria lymphocytes from newborn, 1.5-week-old, and 3-week-old calves were compared to determine to what extent the mucosa becomes populated after birth. Single and dual fluorescence flow cytometry were used with monoclonal antibodies to bovine (Bo) CD molecules to identify lymphocyte subpopulations. Few ileal mucosal lymphocytes were present in calves at birth. However, by 1.5 weeks of age, the villi were populated with large numbers of lymphocytes, and by 3 weeks of age, the numbers had increased further. These included a prominent subpopulation of gamma delta T cells. Several subsets of gamma delta T cells populated ileal mucosa after birth. The predominant subset coexpressed BoCD2, and a smaller subset coexpressed BoCD8. WC1+ gamma delta T cells comprised the smallest subset. All gamma delta T cell subsets coexpressed ACT2, a molecule expressed on activated WC1+ and WC1- gamma delta T cells from cattle.
Assuntos
Movimento Celular/imunologia , Íleo/imunologia , Mucosa Intestinal/imunologia , Receptores de Antígenos de Linfócitos T gama-delta/análise , Subpopulações de Linfócitos T/classificação , Animais , Animais Recém-Nascidos , Bovinos , MasculinoRESUMO
Microbicidal activity of reactive oxygen intermediates and reactive nitrogen intermediates has been described from both murine and human cytokine activated macrophages. An L-arginine-dependent pathway of nitric oxide generation has recently been described from bovine bone marrow-derived and monocyte-derived macrophages in response to a phagocytic stimulus. We have investigated the induction and release of both reactive oxygen intermediates and reactive nitrogen intermediates from bovine neutrophils, and blood and spleen mononuclear phagocytes in response to either a phagocytic or cytokine stimulus. Mononuclear phagocytes were poor producers of hydrogen peroxide (a measure of reactive oxygen intermediate production) under conditions that readily caused release by neutrophils. In contrast, nitrite, as a measure of nitric oxide production, could not be induced from neutrophils under any stimulation conditions, while mononuclear phagocytes responded to both a phagocytic stimulus and cytokines with the induction of nitric oxide synthase message and production of nitric oxide. There appeared to be two populations of monocytes that differed both in their adherent characteristics and their level of cytokine-induced nitric oxide production. Both populations stained with a single monoclonal antibody. However, the population that had not adhered to plastic within 3 h responded to cytokine stimulation, producing up to 3 times more nitric oxide on a per cell basis than the readily adherent population. Cytokine induction required the presence of interferon-gamma and either tumor necrosis factor-alpha or lipopolysaccharide. L-arginine dependence was demonstrated by inhibition with an L-arginine analog and restoration with addition of excess L-arginine.
Assuntos
Arginina/fisiologia , Monócitos/metabolismo , Neutrófilos/metabolismo , Óxido Nítrico/biossíntese , Animais , Bovinos , Sinergismo Farmacológico , Humanos , Interferon gama/farmacologia , Lipopolissacarídeos/farmacologia , Masculino , Espécies Reativas de Oxigênio/metabolismo , Baço/citologia , Fator de Necrose Tumoral alfa/farmacologiaRESUMO
Ileal intraepithelial lymphocyte (IEL) suspensions from suckling calves (1-3 weeks old) and weaned calves (3-6 months old) were phenotyped to determine whether there were differences in the lymphocyte populations consistent with postnatal maturation of the mucosal immune system. Flow cytometric comparisons of IEL from the two age groups revealed the presence of significantly larger proportions of CD4+ T lymphocytes and CD8+ T cells in the weaned animals. In contrast, there was a significantly larger proportion of B-B2+ IEL in the suckling calves. Freshly isolated IEL from both groups of calves expressed mRNA for TNF-alpha and IFN-gamma, but not IL-4 or IL-10. The B-B2+ IEL population was more closely examined by flow cytometry. These cells co-expressed IgM and CD21. However, they did not express IgA, IgG1, nor any of several additional leukocyte differentiation molecules. Immunohistochemical data confirmed the presence of IgM+ lymphocytes, and the paucity of IgA+ and IgG1+ lymphocytes in suckling calf ileum. However, substantial numbers of IgA+ and IgG1+ cells were observed in weaned calf ileum. Together, the data are consistent with ongoing postnatal maturation of the gut mucosal immune system.
Assuntos
Animais Lactentes , Bovinos/crescimento & desenvolvimento , Íleo/crescimento & desenvolvimento , Mucosa Intestinal/crescimento & desenvolvimento , Linfócitos/citologia , Desmame , Animais , Linfócitos B/citologia , Linfócitos B/imunologia , Linfócitos T CD4-Positivos/citologia , Linfócitos T CD8-Positivos/citologia , Íleo/citologia , Imunoglobulina A/análise , Imunoglobulina G/análise , Mucosa Intestinal/citologia , Masculino , FenótipoRESUMO
Thirty-eight foals with combined immunodeficiency (CID) received transplanted fetal liver cells, fetal liver and thymus cells, histocompatible bone marrow cells, or equine lymphocyte antigen (ELA) haploidentical bone marrow cells in an attempt to reconstitute their deficient immune systems. Engraftment was infrequent, partial, and unpredictable when fetal cells were employed. Three of five CID foals receiving ELA haploidentical bone marrow cells demonstrated partial reconstitution, but engraftment was only temporary. Administration of histocompatible bone marrow cells resulted in rapid, full and sustained engraftment.
Assuntos
Doenças dos Cavalos/terapia , Cavalos/imunologia , Síndromes de Imunodeficiência/veterinária , Tecido Linfoide/transplante , Animais , Transplante de Medula Óssea , Histocompatibilidade , Doenças dos Cavalos/imunologia , Cavalos/embriologia , Síndromes de Imunodeficiência/imunologia , Síndromes de Imunodeficiência/terapia , Fígado/embriologia , Transplante de Fígado , Timo/embriologia , Timo/transplanteRESUMO
gamma delta T cells in ruminants can be subdivided in two or more subpopulations on the basis of the expression of surface antigen WC1, which can exist in different isoforms. In this study, 18 monoclonal antibodies (mAbs) submitted to the Third International Workshop that were predicted to react with gamma delta TcR molecules were analysed and expression of their antigens was investigated on the different gamma delta T cell subpopulations. A set of control mAbs positive for TcR1 (86D), BoCD3 (MM1A), WC1 (B7A1, BAQ4A, CACTB32A, and BAQ89A) was included for comparative studies. Previous investigations demonstrated eight of the mAbs immunoprecipitated peptides with apparent M(r)s of 37 and/or 47 kDa, indicating they recognized determinants on the T cell receptor, TcR1. Two color flow cytometric analyses in the present study demonstrated the mAbs formed three groups; group 1, a set of mAbs that recognize TcR1 determinants expressed on all gamma delta T cells and groups 2 and 3, sets of mAbs that recognize TcR1 determinants on some gamma delta T cells: TcR1-N6 and TcR1-N7 respectively. mAbs from the latter groups define families of TcR1 molecules that express either one or both of the determinants. These antigenically distinct forms of TcR1 are expressed in equal proportion on the two gamma delta T cell populations that express one of the mutually exclusive isoforms of WC1, WC1-N3 and WC1-N4. The data indicate usage of the mAb-defined families of the gamma delta TcR is primarily restricted to the WC1+ subpopulation of gamma delta T cells. However, a small subpopulation of CD2+, WC1- gamma delta T cells expresses a form of TcR1 positive for the determinant TcR1-N6.