Observations of Rogue Seas in the Southern Ocean.

We report direct observations of surface waves from a stereo camera system along with concurrent measurements of wind speed during an expedition across the Southern Ocean in the austral winter aboard the South African icebreaker S.A. Agulhas II. Records include water surface elevation across a range of wave conditions spanning from early stages of wave growth to full development. We give experimental evidence of rogue seas, i.e., sea states characterized by heavy tails of the probability density function well beyond the expectation based on bound mode theory. These conditions emerge during wave growth, where strong wind forcing and high nonlinearity drive wave dynamics. Quasiresonance wave-wave interactions, which are known to sustain the generation of large amplitude rogue waves, capture this behavior. Wave statistics return to normality as the wind forcing ceases and waves switch to a full developed condition.

Wind Generated Rogue Waves in an Annular Wave Flume.

We investigate experimentally the statistical properties of a wind-generated wave field and the spontaneous formation of rogue waves in an annular flume. Unlike many experiments on rogue waves where waves are mechanically generated, here the wave field is forced naturally by wind as it is in the ocean. What is unique about the present experiment is that the annular geometry of the tank makes waves propagating circularly in an unlimited-fetch condition. Within this peculiar framework, we discuss the temporal evolution of the statistical properties of the surface elevation. We show that rogue waves and heavy-tail statistics may develop naturally during the growth of the waves just before the wave height reaches a stationary condition. Our results shed new light on the formation of rogue waves in a natural environment.

Experimental Observation and Theoretical Description of Multisoliton Fission in Shallow Water.

We observe the dispersive breaking of cosine-type long waves [Phys. Rev. Lett. 15, 240 (1965)] in shallow water, characterizing the highly nonlinear "multisoliton" fission over variable conditions. We provide new insight into the interpretation of the results by analyzing the data in terms of the periodic inverse scattering transform for the Korteweg-de Vries equation. In a wide range of dispersion and nonlinearity, the data compare favorably with our analytical estimate, based on a rigorous WKB approach, of the number of emerging solitons. We are also able to observe experimentally the universal Fermi-Pasta-Ulam recurrence in the regime of moderately weak dispersion.

Modulational instability, wave breaking, and formation of large-scale dipoles in the atmosphere.

We use direct numerical simulation of the Navier-Stokes equations for a two-phase flow (water and air) to study the dynamics of the modulational instability of free surface waves and its contribution to the interaction between the ocean and atmosphere. If the steepness of the initial wave exceeds a threshold value, we observe wave-breaking events and the formation of large-scale dipole structures in the air. Because of the multiple steepening and breaking of the waves under unstable wave packets, a train of dipoles is released in the atmosphere; those dipoles propagate at a height comparable with the wavelength. The amount of energy dissipated by the breaker in water and air is considered, and contrary to expectations, we observe that the energy dissipation in air is greater than that in water. The possible consequences on the wave modeling and on the exchange of aerosols and gases between air and water are discussed.

Hydrodynamic supercontinuum.

We report the experimental observation of multi-bound-soliton solutions of the nonlinear Schrödinger equation (NLS) in the context of hydrodynamic surface gravity waves. Higher-order N-soliton solutions with N=2, 3 are studied in detail and shown to be associated with self-focusing in the wave group dynamics and the generation of a steep localized carrier wave underneath the group envelope. We also show that for larger input soliton numbers, the wave group experiences irreversible spectral broadening, which we refer to as a hydrodynamic supercontinuum by analogy with optics. This process is shown to be associated with the fission of the initial multisoliton into individual fundamental solitons due to higher-order nonlinear perturbations to the NLS. Numerical simulations using an extended NLS model described by the modified nonlinear Schrödinger equation, show excellent agreement with experiment and highlight the universal role that higher-order nonlinear perturbations to the NLS play in supercontinuum generation.

Experimental observation of dark solitons on the surface of water.

We present the first ever observation of dark solitons on the surface of water. It takes the form of an amplitude drop of the carrier wave which does not change shape in propagation. The shape and width of the soliton depend on the water depth, carrier frequency, and the amplitude of the background wave. The experimental data taken in a water tank show an excellent agreement with the theory. These results may improve our understanding of the nonlinear dynamics of water waves at finite depths.

Equilibrium and nonequilibrium description of negative temperature states in a one-dimensional lattice using a wave kinetic approach.

We predict negative temperature states in the discrete nonlinear Schödinger (DNLS) equation as exact solutions of the associated wave kinetic equation. Within the wave kinetic approach, we define an entropy that results monotonic in time and reaches a stationary state, that is consistent with classical equilibrium statistical mechanics. We also perform a detailed analysis of the fluctuations of the actions at fixed wave numbers around their mean values. We give evidence that such fluctuations relax to their equilibrium behavior on a shorter timescale than the one needed for the spectrum to reach the equilibrium state. Numerical simulations of the DNLS equation are shown to be in agreement with our theoretical results. The key ingredient for observing negative temperatures in lattices characterized by two invariants is the boundedness of the dispersion relation.

Why there is a need of an ethics committee in scientific medical societies.

Scientific associations involved in clinical activities are responsible to their members and to the medical community when using new drugs or technologies in clinical practice. Any new procedure or clinical use of new drugs represents a form of research and should adhere to guidelines defined by an ethics committee. It is therefore important that all medical scientific societies should establish an ethics committee. The ethics committee should be in direct contact with the scientific or research committee of the society to evaluate if a clinical trial is useful and not dangerous for patients. A group of expert members of this committee should advise the best method to avoid harm or discomfort to the patient. This gives the best guarantee and can evaluate the real progress of such a new procedure for the benefit of the patient.

Spontaneous emergence of rogue waves in partially coherent waves: A quantitative experimental comparison between hydrodynamics and optics.

Rogue waves are extreme and rare fluctuations of the wave field that have been discussed in many physical systems. Their presence substantially influences the statistical properties of a partially coherent wave field, i.e., a wave field characterized by a finite band spectrum with random Fourier phases. Their understanding is fundamental for the design of ships and offshore platforms. In many meteorological conditions waves in the ocean are characterized by the so-called Joint North Sea Wave Project (JONSWAP) spectrum. Here we compare two unique experimental results: the first one has been performed in a 270 m wave tank and the other in optical fibers. In both cases, waves characterized by a JONSWAP spectrum and random Fourier phases have been launched at the input of the experimental device. The quantitative comparison, based on an appropriate scaling of the two experiments, shows a very good agreement between the statistics in hydrodynamics and optics. Spontaneous emergence of heavy tails in the probability density function of the wave amplitude is observed in both systems. The results demonstrate the universal features of rogue waves and provide a fundamental and explicit bridge between two important fields of research. Numerical simulations are also compared with experimental results.

Modulation Instability and Phase-Shifted Fermi-Pasta-Ulam Recurrence.

Instabilities are common phenomena frequently observed in nature, sometimes leading to unexpected catastrophes and disasters in seemingly normal conditions. One prominent form of instability in a distributed system is its response to a harmonic modulation. Such instability has special names in various branches of physics and is generally known as modulation instability (MI). The MI leads to a growth-decay cycle of unstable waves and is therefore related to Fermi-Pasta-Ulam (FPU) recurrence since breather solutions of the nonlinear Schrödinger equation (NLSE) are known to accurately describe growth and decay of modulationally unstable waves in conservative systems. Here, we report theoretical, numerical and experimental evidence of the effect of dissipation on FPU cycles in a super wave tank, namely their shift in a determined order. In showing that ideal NLSE breather solutions can describe such dissipative nonlinear dynamics, our results may impact the interpretation of a wide range of new physics scenarios.

Activation of the granulocyte-monocyte colony stimulating factor gene in acute myeloid leukaemia cells is not related to gene rearrangement.

Several reports have documented that leukaemic blasts produce a number of cytokines among them the granulocyte-monocyte colony stimulating factor (GM-CSF). We analysed the structure of the gene that codes for GM-CSF in 44 acute myeloid leukaemia (AML) cases in an attempt to establish whether the autocrine production of GM-CSF was due to a structural gene alteration. No structural alteration was detected in the GM-CSF gene in any of the 44 cases studied. We, therefore, conclude that the autocrine production of GM-CSF by leukaemia blasts is not dependent on gene rearrangement.

Risk factors for colonization or infection due to methicillin-resistant Staphylococcus aureus in HIV-positive patients: a retrospective case-control study.

OBJECTIVE: To determine the risk factors for colonization or infection with methicillin-resistant Staphylococcus aureus in human immunodeficiency virus (HIV)-infected patients. DESIGN: Retrospective matched-pair case-control study. SETTING: Continuity clinic and inpatient HIV service of a university medical center. POPULATION: Patients with HIV infection from the general population of eastern and coastal Texas and from the Texas Department of Criminal Justice. DATA COLLECTION: Patient charts and the AIDS Care and Clinical Research Program Database were reviewed for the following: age, race, number of admissions, total hospital days, presence of a central venous catheter, serum albumin, total white blood cell count and absolute neutrophil count, invasive or surgical procedures, any cultures positive for S. aureus, and a history of opportunistic illnesses, diabetes, or dermatologic diagnoses. Data also were collected on the administration of antibiotics, antiretroviral therapy, steroids, cancer chemotherapy, and subcutaneous medications. RESULTS: In the univariate analysis, the presence of a central venous catheter, an underlying dermatologic disease, lower serum albumin, prior steroid therapy, and prior antibiotic therapy, particularly antistaphylococcal therapy or multiple courses of antibiotics, were associated with increased risk for colonization or infection with methicillin-resistant S. aureus. Multivariate analysis yielded a model that included presence of a central venous catheter, underlying dermatologic disease, broad-spectrum antibiotic exposure, and number of hospital days as independent risk factors for colonization or infection with methicillin-resistant S. aureus. CONCLUSIONS: In our HIV-infected patient population, prior hospitalization, exposure to broad-spectrum antibiotics, presence of a central venous catheter, and dermatologic disease were risk factors for acquisition of methicillin-resistant S. aureus.

Synthesis of enantio-manoyl oxides: modifiers of the activity of adenylatecyclase enzyme.

Cyclization of methyl ent-8 alpha-hydroxylabd-13(16),14-dien-18-oate with m-chloroperbenzoic acid gave methyl (13S)-ent-16-hydroxy-8 alpha,13-epoxylabd-14-en-18-oate and its epimer at C-13. Biotransformation of the former (which exhibits antileishmania activity) with Rhizopus nigricans cultures produced the methyl (13S)-ent-11 beta,16-dihydroxy-8 alpha,13-epoxilabd-14-en-18-oate (carbomanoyl, which inhibits the activity of the adenylatecyclase enzyme), methyl (13S)-ent-3 beta,16-dihydroxy-8 alpha,13-epoxilabd-14-en-18-oate, methyl (13S)-ent-3 beta,11 beta,16-trihydroxy-8 alpha,13-epoxilabd-14-en-18-oate and the (14S)-ent-3 beta-hydroxy-14,15-epoxy derivative that cyclized spontaneously to a spiran compound. Biotransformation of methyl (13S)-ent-16-hydroxy-3-oxo-8 alpha,13-epoxilabd-14-en-18-oate with R. nigricans produced ent-11 beta-hydroxylation, reduction of the keto group at C-3 (to give 3S-alcohol) and 14(S),15-epoxidation, which also rearranged to a spiro compound.

In vitro production of leukemia inhibitory factor (LIF) by Hep G2 hepatoblastoma cells.

This study shows that the human hepatoblastoma cell line Hep G2 constitutively expressed a high level of Leukemia Inhibitory Factor (LIF) mRNA in the characteristic major 3.8 and minor 1.8 Kb forms. DNA analysis of the LIF gene from Hep G2 revealed no rearrangements. Production and secretion of significant concentrations of LIF were demonstrated by enzyme-linked immunoabsorbent assay (ELISA) in culture supernatants of Hep G2 cells. The highest LIF concentration in culture was found at 48-h (250 pg/ml). LIF produced by Hep G2 cells was biologically active since cell-free culture supernatants were able to induce in vitro differentiation of the M1 murine myeloid leukemia cell line. On the contrary, no LIF mRNA expression was detected in normal liver cells by PCR analysis. Our results suggest that LIF acts on normal parenchymal hepatocytes through a paracrine mechanism and on Hep G2 cells by an autocrine action. Furthermore they indicate that the Hep G2 cell line could be an useful model for studying the LIF autocrine mechanism in hepatomas.

Testicular atrophy in 80 HIV-positive patients: a multivariate statistical analysis.

This retrospective study of risk factors for testicular atrophy in HIV-infected men investigates the relationship between complications of AIDS such as wasting or opportunistic illness and testicular atrophy. Microscopic sections of the right testis were evaluated for testicular atrophy by assessing the mean score in each of 80 selected HIV-infected patients who underwent an autopsy during a one-year period. A significant association was observed between testicular atrophy and body mass index (BMI) (P=0.0496). Thus, underweight patients with HIV infection were 3.52 times more likely to have testicular atrophy than those with acceptable body weight. Other significant associations between other variables were not found.

Gallstones in elderly patients: impact of laparoscopic cholecystectomy.

The use of laparoscopic cholecystectomy (LC) in elderly patients may pose problems because of their poor general condition, especially of cardiopulmonary function. Moreover, these patients present with acute cholecystitis and associated common bile duct stones more often than their younger counterparts. From 1990 to 1999, the authors performed 943 LCs; 31 (3.2%) were attempted on elderly patients, 11 (35%) of which were on an emergency basis because of acute cholecystitis, cholangitis or acute biliary pancreatitis. Ten per cent of LCs needed to be converted to an open cholecystectomy, most often because of an increase in the partial pressure of carbon dioxide in the blood produced by excessive operative time. A gasless procedure was used in the last three years of the study on eight cases; the overall rate of conversion from LC to open cholecystectomy in this group was 0%. Associated gallbladder and common bile duct stones were found in five (16%) patients (four preoperative LC endoscopic sphincterotomy and one transcystic approach). The success rate in both of these cases was 100%, overall morbidity was 29% and there was no mortality. These results show that LC is a feasible and safe procedure for use in elderly patients. Gasless LC should be preferred in patients classified as American Society of Anesthesiologists' class III because an excessive duration of operation is the most common reason for converting to an open cholecystectomy.

Probability density function and "plus" and "minus" structure functions in a turbulent channel flow.

We consider the statistical properties of the longitudinal velocity increments in a turbulent channel flow at different distances from the wall. The probability density function (PDF) of the velocity difference of the streamwise component near the wall are found to be, especially at small scales, strongly skewed, showing a very long left tail. We consider "plus" and "minus" structure functions and compute separately the statistics for the right and left part of the PDF. It is found that the relative scaling exponents for the right tail are less affected by the presence of the wall and their values are consistent with the ones found in experiments in homogeneous and isotropic turbulence. A simple phenomenological model that explains the results obtained is also given.

Observation of strongly non-Gaussian statistics for random sea surface gravity waves in wave flume experiments.

We study random surface gravity wave fields and address the formation of large-amplitude waves in a laboratory environment. Experiments are performed in one of the largest wave tank facilities in the world. We present experimental evidence that the tail of the probability density function for wave height strongly depends on the Benjamin-Feir index (BFI)-i.e., the ratio between wave steepness and spectral bandwidth. While for a small BFI the probability density functions obtained experimentally are consistent with the Rayleigh distribution, for a large BFI the Rayleigh distribution clearly underestimates the probability of large events. These results confirm experimentally the fact that large-amplitude waves in random spectra may result from the modulational instability.

[Endolaparoscopic "Rendez-vous" in the treatment of cholecysto-choledochal calculosis]. / Il "Rendez-vous" endolaparoscopico nel trattamento della calcolosi colecisto-coledocica.

Different methods, all of which equally efficacious and safe, can be selected to access the choledochus in patients with cholecysto and choledocholithiasis on the basis of clinical and anatomosurgical parameters. From 1990 we evaluated three groups of patients who underwent surgery at different times and with different methods: sequentially (endoscopic sphincterotomy and laparoscopic cholecystectomy), one step laparoscopy and combined laparo-endoscopy. The results obtained seem to show that the treatment with laparoscopy alone is the most advantageous in terms of cost-benefit, while the endoscopic access of the choledochus during laparoscopic cholecystectomy is the one to prefer in terms of efficacy and safety.

[A case of anaplastic carcinoma of the thyroid. Clinico-pathological and therapeutic aspects and review of the literature]. / Un caso di carcinoma anaplastico della tiroide. Aspetti clinico-patologici, terapeutici e revisione della Letteratura.

A case of anaplastic carcinoma of the thyroid observed in a 72-year-old female patient, admitted with previous diagnosis of Riedel's thyroiditis, is reported. Clinicopathologic features and management of this rare neoplasm are discussed and Literature is reviewed. Anaplastic cancer is a locally and systemically aggressive histologic type, occurring more frequently in older patients and in those with a history of benign and/or malignant thyroid disease. Because long-term survival was seen most commonly in well-localized anaplastic tumors, a more aggressive approach to thyroid lesions, especially in the elderly, may be warranted to improve early diagnosis and effectiveness of therapy of this nearly always lethal neoplasm. Although patients can rarely be cured, efforts should be made to control the disease locally by combined treatment including surgery and radiochemotherapy, which is found to be, at times, a national palliative therapeutic approach despite patient's advanced age.