RESUMO
We have identified VPS33B, the human ortholog of rat Vps33b. VPS33B encodes a transcript of 2482 nt with an ORF of 617 amino acids and a predicted protein size of 70.6 kDa. VPS33B contains a Sec-1 domain shared with a family of proteins involved in protein sorting and vesicular trafficking. Enriched expression of VPS33B was observed in testis. VPS33B was positioned at chromosome 15q26.1 by radiation hybrid mapping.
Assuntos
Cromossomos Humanos Par 15/genética , Proteínas de Membrana , Proteínas/genética , Sequência de Aminoácidos , Animais , Mapeamento Cromossômico , Clonagem Molecular , Etiquetas de Sequências Expressas , Perfilação da Expressão Gênica , Humanos , Células Híbridas , Escore Lod , Masculino , Dados de Sequência Molecular , Fases de Leitura Aberta/genética , Estrutura Terciária de Proteína , Proteínas/química , RNA Mensageiro/análise , RNA Mensageiro/genética , Ratos , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos , Sitios de Sequências Rotuladas , Testículo/metabolismo , Proteínas de Transporte VesicularRESUMO
We have isolated C15orf3, a novel human gene that lacks homology to any known gene family. The C15orf3 gene encodes a transcript of 1676 nt with an ORF of 187 amino acids and a predicted protein product size of 20.8 kDa. Northern blot analysis showed ubiquitous expression in adult tissues. EST database searching revealed the presence of C15orf3 homologs in rat and mouse. C15orf3 was mapped to chromosome 15q21.1-->q21.2 using the Stanford G3 radiation hybrid panel.
Assuntos
Proteínas E1A de Adenovirus , Cromossomos Humanos Par 15/genética , Fases de Leitura Aberta/genética , Proteínas/genética , Sequência de Aminoácidos , Animais , Proteínas de Ciclo Celular , Mapeamento Cromossômico , Clonagem Molecular , Etiquetas de Sequências Expressas , Perfilação da Expressão Gênica , Humanos , Células Híbridas , Escore Lod , Camundongos , Dados de Sequência Molecular , Peso Molecular , Proteínas Nucleares , Proteínas/química , RNA Mensageiro/análise , RNA Mensageiro/genética , Ratos , Proteínas Repressoras , Alinhamento de Sequência , Homologia de Sequência de AminoácidosRESUMO
We report the sequence, tissue distribution, and chromosome location of a novel gene, PDCD9 (programmed cell death 9). PDCD9 is the mammalian counterpart of the Gallus gallus pro-apoptotic protein p52. The human cDNA has an open reading frame of 1,314 nucleotides and was predicted to encode a protein of 438 amino acids with a calculated mass of 50 kDa. The protein sequences of chicken, mouse, and human PDCD9 are remarkably conserved. PDCD9 mRNA is expressed ubiquitously in adult tissues, displaying a stronger signal in heart, skeletal muscle, kidney, and liver. PDCD9 was mapped to chromosome 5q11.
Assuntos
Proteínas de Ciclo Celular/química , Proteínas de Ciclo Celular/genética , Galinhas/genética , Mapeamento Físico do Cromossomo , Proteínas Virais/química , Sequência de Aminoácidos , Animais , Apoptose/genética , Proteínas de Ciclo Celular/metabolismo , Cromossomos Humanos Par 5/genética , Clonagem Molecular , Sequência Conservada/genética , Etiquetas de Sequências Expressas , Humanos , Hibridização in Situ Fluorescente , Camundongos , Dados de Sequência Molecular , RNA Mensageiro/análise , RNA Mensageiro/genética , Alinhamento de Sequência , Homologia de Sequência de AminoácidosRESUMO
The HMG box encodes a conserved DNA binding domain found in many proteins and is involved in the regulation of transcription and chromatin conformation. We describe HMG20A and HMG20B, two novel human HMG box-containing genes, discovered within the EURO-IMAGE Consortium full-length cDNA sequencing initiative. The predicted proteins encoded by these two genes are 48.4% identical (73.9% within the HMG domain). The HMG domain of both HMG20 proteins is most similar to that of yeast NHP6A (38% to 42%). Outside of this domain, HMG20 proteins lack any significant homology to other known proteins. We determined the genomic structure and expression pattern of HMG20A and HMG20B. Both genes have several alternative transcripts, expressed almost ubiquitously. HMG20A maps to chromosome 15q24 (near D15S1227) and HMG20B to 19p13.3 (between D19S209 and D19S216). The HMG20 genes define a distinct class of mammalian HMG box genes.