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Lippia (Phyla canescens) is a fast-growing, mat-forming, and prostrate perennial plant well adapted to infertile, high-saline, and drought environments (Leigh, et al. 2004). It arrived in China from Japan as a flowering ground cover in 2001 (Cai, et al. 2004). In June 2022, southern blight appeared in our nursery of the Floriculture Research Institute of Guangdong Academy of Agricultural Sciences. High temperature and damp environment are major factors for this disease. The symptoms of top-layer plants were not easily detected, but they were slightly yellowed. A yellowish-brown water-soak lesion appeared on the stems and lowest leaves exposed to soil. White mycelium appeared in the middle stage. Finally, the surface plants showed water-soak decay, and a mass of beige to black-brown rapeseed-shaped sclerotia appeared on the residue and surrounding soil; these plants died. Sclerotia and mycelia were collected from disease tissue, and after surface sterilization, sclerotia was cultured on potato dextrose agar (PDA) at 28±2°C in an incubator without light. Eight fungal isolates with similar colony morphologies were consistently isolated by purifying from different sampling areas. The isolates exhibited obvious septa and a clamp connection structure within the white mycelium. The average growth rate was 26.86±0.06 mm/day. Numerous white granular sclerotia were produced on the mycelium 6 days later. The sclerotia with a diameter of 1.24±0.07mm (n=189) gradually changed from diage to yellow to brown. A typical strain B1 was selected for further identification, targeting its 18S rRNA and LSU rRNA sequences (Yang, et al. 2011; Xue, et al. 2019). Its 18S rRNA sequence (GenBank Accession No. OR517233, 1626 bp) is 99.63% and 99.57% identical to Athelia rolfsii (AY665774, 1179bp; KC670714, 1775bp; JF819726, 1781bp). Its LSU rRNA sequence (OR539570, 757 bp) is 99.87% identical to Agroathelia rolfsii (OR526537, 904 bp). For Athelia rolfsii, a synonym of Agroathelia rolfsii, by combining the morphological characteristics and molecular identification, the isolate pathogen B1 was confirmed to be Agroathelia rolfsii (the teleomorph of Sclerotium rolfsii). To fullfill Koch's postulates, we inoculated the mycelial plugs to healthy lippia stems and leaves which has grown for one year, with PDA plugs free of mycelium as the control. All the plants were kept in a greenhouse at 28±2°C with a 14-h photoperiod and 80% relative humidity. Each treatment was repeated thrice and vaccinated with 6 points. At 7 d following inoculation, all plants inoculated with B1 showed typical symptoms, but the control group was asymptomatic, and sclerotia appeared 17d after inoculation. Using the same protocol mentioned above, pathogenic fungal was reisolated only from treated groups, but not from the control group. Chose three of the pathogens for 18S rRNA and LSU rRNA sequencing, the results showed 100% identity to B1, the same as its microstructure. There are few reports about the disease on P. canescens. Sosa (2007) investigated the pathogens on P. canescens in Argentina, 16 fungi were found but no A. rolfsii. Sclerotium rolfsii were identified on P. nodiflora or P. lanceolata (Michaux) Greene in America (Farr, et al. 1989). To our knowledge, this is the first report in China. Because this pathogen has wide-ranging hosts and causes serious damage, the results from this study will offer guidance for the prevention and treatment of this disease.
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In this study, samples of daily foods, drinking waters, surface waters, and soils were collected and screened to investigate the external exposure of Sb and As from various intake pathways in typical Sb mining area. Biomarker samples of residents were analyzed to monitor internal exposure characteristic of Sb and As in human body. Exposure dosages of As and Sb and transfer of Sb and As from environment to human body were estimated based on the external and internal exposure. The following results were obtained: daily intakes of food accounted for major intakes of both Sb and As, and highlighted the significance of foods intakes from rice and vegetable. The results of Monte Carlo simulations showed that total daily intake of Sb(n = 1444)and As(n = 1131) approximately reached 1.08 × 10-2 mg/kg/d and 1.19 × 10-3 mg/kg/d, in which 98.82% and 63.07% of residents have exceeded the threshold dosages of Sb and As. The contaminants contents in biomarkers indicated that Sb exhibited the similar internal exposure as As, while the total transfer rate of Sb from environment to human were estimated as approximately 2.04-2.40 times lower than As. This study also suggested that drinking water is another important pathway with high bioavailability and male resident may present higher priority than female in uptake of Sb and As. The paper suggested the similarity and difference on bioavailability existed in Sb and its group V elements, As, that would provide the essential information on exposure of Sb and As in the typical Sb mine area.
Assuntos
Arsênio , Poluentes do Solo , Antimônio/análise , Arsênio/análise , Monitoramento Ambiental , Feminino , Humanos , Masculino , Mineração , Solo , Poluentes do Solo/análiseRESUMO
Climate change not only intensifies eutrophication and enhances the rainfall, but also elevates the contents of greenhouse gases, which can further increase the intensity and frequency of extreme precipitation events. The effectivity of phytoremediation of urban wastewaters by water hyacinths under an extreme rainfall event (up to 380 mm d-1) was investigated using self-designed fabrications with six flow rates (2-15 m3 d-1) in situ on pilot scale for 30 days. The results suggest that water hyacinths had high N and P removal capacities even under adverse conditions such as low dissolved oxygen concentrations (DO, <1 mg L-1) and high ammonium concentrations (NH4+-N, >7 mg L-1). Specifically, the highest removal yields of N and P were 13.14 ± 0.47 g N·m-2·d-1 and 2.12 ± 0.04 g P·m-2·d-1, respectively. The results indicate that water hyacinths can be used for water treatment to reduce the amounts of NH4+-N, dissolved organic nitrogen (DON) and phosphate (PO43-) even during extreme precipitation events. Moreover, DO increased due to wet deposition, runoff and surface flows during the extreme rainfall event, resulting in shifts between nitrification and denitrification processes which significantly altered nitrogen forms in urban wastewater. Results of this study suggest that water hyacinths could be recommended as a cost-effective and eco-friendly technology for urban wastewater phytoremediation in areas suffering from frequent extreme precipitation events.
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Eichhornia , Poluentes Químicos da Água/análise , Biodegradação Ambiental , Nitrogênio/análise , Águas ResiduáriasRESUMO
Biofilms have important effects on nutrient cycling in aquatic ecosystems. However, publications about the community structure and functions under laboratory conditions are rare. This study focused on the developmental and physiological properties of cultured biofilms under various phosphorus concentrations performed in a closely controlled continuous flow incubator. The results showed that the biomass (Chl a) and photosynthesis of algae were inhibited under P-limitation conditions, while the phosphatase activity and P assimilation rate were promoted. The algal community structure of biofilms was more likely related to the colonization stage than with the phosphorus availability. Cyanobacteria were more competitive than other algae in biofilms, particularly when cultured under low P levels. A dominance shift occurred from non-filamentous algae in the early stage to filamentous algae in the mid and late stages under P concentrations of 0.01, 0.1 and 0.6 mg/L. However, the total N content, dry weight biomass and bacterial community structure of biofilms were unaffected by phosphorus availability. This may be attributed to the low respiration rate, high accumulation of extracellular polymeric substances and high alkaline phosphatase activity in biofilms when phosphorus availability was low. The bacterial community structure differed over time, while there was little difference between the four treatments, which indicated that it was mainly affected by the colonization stage of the biofilms rather than the phosphorus availability. Altogether, these results suggested that the development of biofilms was influenced by the phosphorus availability and/or the colonization stage and hence determined the role that biofilms play in the overlying water.
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Biofilmes/crescimento & desenvolvimento , Fósforo/análise , Microbiologia da Água , Poluentes Químicos da Água/análise , Cianobactérias , Ecossistema , Fósforo/metabolismo , Poluentes Químicos da Água/metabolismoRESUMO
In natural habitats, organisms especially phytoplankton are not always continuously subjected to ultraviolet-B radiation (UVBR). By simulation of the natural situation, the N2-fixing cyanobacterium Anabaena sp. PCC 7120 was subjected to UV-B exposure and recovery cycles. A series of morphological and physiological changes were observed in Anabaena sp. PCC 7120 under repeated UVBR when compared with controls. Such as the breakage of filaments, intervals between heterocysts, heterocyst frequency, total carbohydrate, and carotenoids were increased, while the nitrogenase activity and photosynthetic activity were inhibited by repeated UVBR; however, these activities could recover when UV-B stress was removed. Unexpectedly, the over-compensatory growth was observed at the end of the second round of exposure and recovery cycle. Our results showed that discontinuous UVBR could increase the growth rate and the tolerance as well as repair capacity of Anabaena sp. PCC 7120. These results indicate that moderate UVBR may increase the growth of cyanobacteria in natural habitats.
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Anabaena/efeitos da radiação , Regulação Bacteriana da Expressão Gênica/efeitos da radiação , Anabaena/crescimento & desenvolvimento , Anabaena/metabolismo , Metabolismo dos Carboidratos , Carotenoides/metabolismo , Clorofila/metabolismo , Nitrogenase/metabolismo , Raios Ultravioleta , Microbiologia da ÁguaRESUMO
Plant invasions and microplastics (MPs) have significantly altered the structure and function of aquatic habitats worldwide, resulting in severe damage to aquatic ecosystem health. However, the effects of MPs on plant invasion and the underlying mechanisms remain largely unknown. In this study, we conducted mesocosm experiments over a 90-day period to assess the effects of polystyrene microplastics on the invasion of exotic submerged macrophytes, sediment physicochemical properties, and sediment bacterial communities. Our results showed that PS-MPs significantly promoted the performance of functional traits and the invasive ability of exotic submerged macrophytes, while native plants remained unaffected. Moreover, PS-MPs addition significantly decreased sediment pH while increasing sediment carbon and nitrogen content. Additionally, MPs increased the diversity of sediment bacterial community but inhibited its structural stability, thereby impacting sediment bacterial multifunctionality to varying degrees. Importantly, we identified sediment properties, bacterial composition, and bacterial multifunctionality as key mediators that greatly enhance the invasion of exotic submerged macrophytes. These findings provide compelling evidence that the increase in MPs may exacerbate the invasion risk of exotic submerged macrophytes through multiple pathways. Overall, this study enhances our understanding of the ecological impacts of MPs on aquatic plant invasion and the health of aquatic ecosystems.
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Microbiota , Microplásticos , Microplásticos/toxicidade , Ecossistema , Plásticos , Poliestirenos , BactériasRESUMO
Agricultural biomass used as solid carbon substrates in ecological floating beds (EFBs) has been proven to be applicable in nitrogen removal for carbon-limited wastewater treatment. However, the subtle interactions among plants, rhizosphere microorganisms, and supplementary carbon sources have not been thoroughly studied. This study combined rice straw mats with different aquatic macrophytes in EFBs to investigate denitrification efficiency in carbon-limited eutrophic waters. Results showed that rice straw significantly enhanced the nitrogen removal efficiency of EFBs, while enriching nitrogen-fixing and denitrifying bacteria (such as Rhizobium, Rubrivivax, and Rhodobacter, etc.). Additionally, during the denitrification process in EFBs, rice straw can release humic acid-like fraction as electron donors to support the metabolic activities of microorganisms, while aquatic macrophytes provide a more diverse range of dissolved organic matters, facilitating a sustainable denitrification process. These findings help to understand the synergistic effect of denitrification processes within wetland ecosystems using agricultural biomass.
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Carbono , Desnitrificação , Nitrogênio , Oryza , Águas Residuárias , Águas Residuárias/química , Purificação da Água/métodos , Biomassa , Bactérias/metabolismo , Áreas Alagadas , Biodegradação AmbientalRESUMO
Cyanobacteria can reach high densities in eutrophic lakes, which may cause problems due to their potential toxin production. Several methods are in use to prevent, control or mitigate harmful cyanobacterial blooms. Treatment of blooms with low concentrations of hydrogen peroxide (H2O2) is a promising emergency method. However, effects of H2O2 on cyanobacteria, eukaryotic phytoplankton and zooplankton have mainly been studied in controlled cultures and mesocosm experiments, while much less is known about the effectiveness and potential side effects of H2O2 treatments on entire lake ecosystems. In this study, we report on three different lakes in the Netherlands that were treated with average H2O2 concentrations ranging from 2 to 5 mg L-1 to suppress cyanobacterial blooms. Effects on phytoplankton and zooplankton communities, on cyanotoxin concentrations, and on nutrient availability in the lakes were assessed. After every H2O2 treatment, cyanobacteria drastically declined, sometimes by more than 99%, although blooms of Dolichospermum sp., Aphanizomenon sp., and Planktothrix rubescens were more strongly suppressed than a Planktothrix agardhii bloom. Eukaryotic phytoplankton were not significantly affected by the H2O2 additions and had an initial advantage over cyanobacteria after the treatment, when ample nutrients and light were available. In all three lakes, a new cyanobacterial bloom developed within several weeks after the first H2O2 treatment, and in two lakes a second H2O2 treatment was therefore applied to again suppress the cyanobacterial population. Rotifers strongly declined after most H2O2 treatments except when the H2O2 concentration was ≤ 2 mg L-1, whereas cladocerans were only mildly affected and copepods were least impacted by the added H2O2. In response to the treatments, the cyanotoxins microcystins and anabaenopeptins were released from the cells into the water column, but disappeared after a few days. We conclude that lake treatments with low concentrations of H2O2 can be a successful tool to suppress harmful cyanobacterial blooms, but may negatively affect some of the zooplankton taxa in lakes. We advise pre-tests prior to the treatment of lakes to define optimal treatment concentrations that kill the majority of the cyanobacteria and to minimize potential side effects on non-target organisms. In some cases, the pre-tests may discourage treatment of the lake.
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Cianobactérias , Fitoplâncton , Animais , Peróxido de Hidrogênio , Lagos/microbiologia , Zooplâncton , Ecossistema , Cianobactérias/fisiologiaRESUMO
Geosmin has been commonly detected both in various aquatic environments and biota, but its exact toxicological mechanisms to organisms need further experimentation. In the present study, zebrafish embryos were exposed to geosmin at nominal concentrations of 50, 500 and 5000 ng/L for 120 h post-fertilization (hpf), followed by locomotor activity and biochemical parameter examination, and multi-omics investigation of the transcriptome and metabolome. The results showed that geosmin exposure significantly reduced the mitochondrial electron transport chain (ETC) complexes I-V, ATP content and mitochondrial respiration and suppressed the locomotor behavior of zebrafish larvae. Transcriptomics analysis revealed that the transcripts of genes involved in oxidative phosphorylation, glycolysis, and lipid metabolism were significantly affected, indicating that geosmin disrupts energy metabolism. Furthermore, metabolomics results showed that 3 classes of lipids, namely glycerophospholipids (GPs), sphingolipids (SLs) and fatty acyls (FAs) were significantly decreased after geosmin exposure. This study provides novel insight into the underlying mechanisms of geosmin-induced energy metabolism and highlights the need for concern about geosmin exposure.
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Poluentes Químicos da Água , Peixe-Zebra , Animais , Peixe-Zebra/metabolismo , Estresse Oxidativo , Metabolismo Energético , Larva , Mitocôndrias/metabolismo , Embrião não Mamífero/metabolismo , Poluentes Químicos da Água/toxicidade , Poluentes Químicos da Água/metabolismoRESUMO
Rising atmospheric CO2 can intensify harmful cyanobacterial blooms in eutrophic lakes. Worldwide, these blooms are an increasing environmental concern. Low concentrations of hydrogen peroxide (H2O2) have been proposed as a short-term but eco-friendly approach to selectively mitigate cyanobacterial blooms. However, sensitivity of cyanobacteria to H2O2 can vary depending on the available resources. To find out how cyanobacteria respond to H2O2 under elevated CO2, Microcystis aeruginosa PCC 7806 was cultured in chemostats with nutrient-replete medium under C-limiting and C-replete conditions (150 ppm and 1500 ppm CO2, respectively). Microcystis chemostats exposed to high CO2 showed higher cell densities, biovolumes, and microcystin contents, but a lower photosynthetic efficiency and pH compared to the cultures grown under low CO2. Subsamples of the chemostats were treated with different concentrations of H2O2 (0-10 mg·L-1 H2O2) in batch cultures under two different light intensities (15 and 100 µmol photons m-2·s-1) and the response in photosynthetic vitality was monitored during 24 h. Results showed that Microcystis was more resistant to H2O2 at elevated CO2 than under carbon-limited conditions. Both low and high CO2-adapted cells were more sensitive to H2O2 at high light than at low light. Microcystins (MCs) leaked out of the cells of cultures exposed to 2-10 mg·L-1 H2O2, while the sum of intra- and extracellular MCs decreased. Although both H2O2 and CO2 concentrations in lakes vary in response to many factors, these results imply that it may become more difficult to suppress cyanobacterial blooms in eutrophic lakes when atmospheric CO2 concentrations continue to rise.
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Cianobactérias , Microcystis , Peróxido de Hidrogênio , Dióxido de Carbono/farmacologia , Técnicas de Cultura Celular por LotesRESUMO
Pseudomonas chengduensis is a new species of Pseudomonas discovered in 2014, and currently, there is a scarcity of research on this bacterium. The P. chengduensis strain WD211 was isolated from a fish pond. This study investigated the purification capability and environmental adaptability of strain WD211 in wastewater and described the basic features and functional genes of its complete genome. According to the results, the sewage treated with strain WD211 showed a decrease in concentration of 18.12% in total nitrogen, 89.39% in NH4+, 62.16% in NO3-, 79.97% in total phosphorus, and 71.41% in COD after 24 h. Strain WD211 is able to survive in a pH range of 6-11. It shows resistance to 7% sodium chloride and different types of antibiotics. Genomic analysis showed that strain WD211 may remove nitrogen and phosphorus through the metabolic pathway of nitrogen assimilation and phosphorus accumulation, and that it can promote organic decomposition through oxygenase. Strain WD211 possesses genes for producing betaine, trehalose, and sodium ion transport, which provide it with salt tolerance. It also has genes for antibiotic efflux and multiple oxidases, which give it antibiotic resistance. This study contributes to the understanding of the sewage treatment ability and potential applications of P. chengduensis.
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Pseudomonas , Esgotos , Animais , Esgotos/microbiologia , Pseudomonas/genética , Pseudomonas/metabolismo , Aquicultura , Antibacterianos/metabolismo , Nitrogênio/metabolismo , Fósforo/metabolismoRESUMO
A large number of tailings ponds formed by slag accumulation have become serious environmental hazards. Spatially high potential energy and long-term accumulation may result in gradient-changing seepage pollution. The assemblages of phytoplankton and bacteria are widely used as assessment indicators. In this study, we investigate the changes in phytoplankton and bacterial assemblages in tailing pollution. The results showed that there are temporal and spatial variabilities in seepage pollution. The abundance and diversity of phytoplankton and bacteria decreased with increasing pollution. However, Synedra acus (diatom) and Polynucleobacter (bacteria) were positively correlated with pollution levels (r = 0.37, P < 0.05; r = 0.24, P < 0.05). Heavy metals are the main contributors to bacterial changes (16.46%), while nutrients are for algae (13.24%). Tailings pond pollution reduced the number of phytoplankton and bacterial linkages. However, more pollution broke the originally independent modules of phytoplankton and bacteria, and they produced more positive correlations (79.39%; 87.68%). Microcystis sp. and Limnobacter were the key nodes of the co-occurrence network in the polluted areas. Exploring the interactions between bacteria and phytoplankton within different pollution levels could provide insights into biological interaction patterns and the bioremediation of tailings ponds.
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Metais Pesados , Lagoas , Bactérias , Biodegradação Ambiental , FitoplânctonRESUMO
In nature, the odorous substance ß-ionone has been widely detected in aquatic ecosystems. However, little is known about its ecotoxicological effects on freshwater vertebrates. In this study, we aimed to assess the acute toxicity of ß-ionone in zebrafish (Danio rerio) embryos from 2 to 120 h post fertilization (hpf) and investigate embryo development, locomotor behavior and pigmentation under different concentrations. The results showed that exposure to ß-ionone had an acute toxicity to early life stages of zebrafish and induced a decrease in hatching rate and an increase in the mortality and malformation rate. The median lethal concentration (LC50) of ß-ionone at 96 h was observed as 1321 µg/L. In addition, ß-ionone not only affected the body length of zebrafish larvae but also regulated the transcription of genes and the levels of hormones involved in the growth hormone/insulin-like growth factor (GH/IGF) and the hypothalamic-pituitary-thyroid (HPT) axes. Moreover, exposure to ß-ionone induced significant decreases in locomotor activity and catecholamine neurotransmitters levels. Furthermore, ß-ionone stimulated pigmentation via regulation of tyrosinase activity and melanin-related gene expression. Overall, this research could provide new insights into the potential risk of odorants to aquatic organisms.
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Hiperpigmentação , Poluentes Químicos da Água , Animais , Ecossistema , Embrião não Mamífero , Larva , Norisoprenoides , Poluentes Químicos da Água/metabolismo , Peixe-Zebra/fisiologiaRESUMO
OBJECTIVE: To investigate the effects of Shenfu Injection (, SFI) on endothelial damage in a porcine model of hemorrhagic shock (HS). METHODS: After being bled to a mean arterial pressure of 40±3 mm Hg and held for 60 min, 32 pigs were treated with a venous injection of either shed blood (transfusion group), shed blood and saline (saline group), shed blood and SFI (SFI group) or without resuscitation (sham group). Venous blood samples were collected and analyzed at baseline and 0, 1, 2, 4, and 6 h after HS. Tumor necrosis factor-α (TNF-α), serum interleuking (IL)-6, and IL-10 levels were measured by enzyme-linked immunosorbent assay (ELISA); expressions of vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule 1 (ICAM -1), von Willebrand factor (vWF), plasminogen activator inhibitor-1 (PAI-1) and Bcl-2, Bax, and caspase-3 proteins were determined by Western blot. RESULTS: The serum level of TNF-α in the SFI group was significantly lower than in the other groups at 0, 1, and 2 h after HS, while the level of IL-6 was lower at 4 and 6 h compared with the saline group (P<0.01 or P<0.05). The concentration of serum IL-10 was significantly higher in the SFI group than in the other groups at 0, 1, 4, and 6 h after HS (P<0.01). Western blot and immunohistochemistry of vascular tissue showed that the expression of caspase-3 was downregulated, and that of Bcl-2 and Bax was upregulated in the SFI group compared to other groups (P<0.05). CONCLUSION: SFI attenuated endothelial injury in the porcine model of HS by inhibiting cell apoptosis, suppressing the formation of proinflammatory cytokines, and reducing endothelial activation.
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Choque Hemorrágico , Fator de Necrose Tumoral alfa , Animais , Caspase 3/metabolismo , Medicamentos de Ervas Chinesas , Interleucina-10 , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Choque Hemorrágico/tratamento farmacológico , Suínos , Fator de Necrose Tumoral alfa/metabolismo , Proteína X Associada a bcl-2/metabolismoRESUMO
Precipitation is a driver of changes in the spatiotemporal distribution of phytoplankton communities. The ecological consequence of precipitation is important, but the underlying processes are not clear. Here we conducted an immediate prior- and after-event short-interval investigation in the Three Gorges Reservoir region, to test whether the short-term changes in the phytoplankton communities and functional groups could be predicted based on the precipitation level. We found that precipitation of moderate and high levels immediately changed the phytoplankton distribution and altered functional groups. According to structural equation model, the vertical velocity (λ = -0.81), light availability (Zeu/Zmix, λ = 0.47) and relative water column stability (RWCS, λ = 0.38) were important parameters for phytoplankton distribution during the precipitation event. Water quality did not directly affect phytoplankton distribution (λ = -0.11) and effects of precipitation on the water quality only lasted 1-2 days. The phytoplankton community was redistributed with some tolerance functional groups appearance, such as groups F, Lo, M and groups M, MP, TB, W1 appeared during- and after- precipitation event, respectively. We also found that mixing rather than flushing was the driving force for the decrease of phytoplankton biomass. Our study provided valuable data for reservoir regulation and evidence for predictions of phytoplankton during the precipitation events under different climate change scenarios.
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PURPOSE: Shen-fu injection (SFI) was used to intervene in the resuscitation of porcine hemorrhagic shock (HS) model to study its protective effects on acute kidney injury. METHODS: After 60 min of HS, 28 animals were randomly assigned into four groups. The groups were as follows: hemorrhagic shock group (HS); HS resuscitation with shed-blood group (HSR); HS resuscitation with shed-blood and SFI (1 mL·kg-1) group (HSR-SFI); and the sham operation group (Sham). The bloods were analyzed for serum creatinine (sCr), cystatin C (CysC) and neutrophil gelatinase-associated lipocalin (NGAL). BAX, Bcl-2, and caspase-3 protein expressions by Western blot analysis and immunohistochemical staining. The renal tissues were removed and pathologic changes were observed. RESULTS: Mean aortic pressure (MAP) in HSR-SFI groups were higher than that in HSR groups after shock. At the 6th hour after shock, the urine volume per hour in the HSR-SFI groups was more than that in the HSR groups. The sCr, NGAL, CysC and cytokine levels of HSR-SFI groups were lower. The Bcl-2 expression was increased in the HSR-SFI groups. The BAX and caspase-3 expressions were reduced. The histopathologic score in the HSR-SFI was lower. CONCLUSIONS: SFI may reduce the risk of acute kidney injury (AKI) following hemorrhagic shock by attenuating systemic inflammatory responses, and regulating the expression of apoptosis-related proteins.
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Injúria Renal Aguda , Choque Hemorrágico , Injúria Renal Aguda/tratamento farmacológico , Injúria Renal Aguda/prevenção & controle , Animais , Apoptose , Citocinas , Medicamentos de Ervas Chinesas , Choque Hemorrágico/tratamento farmacológico , SuínosRESUMO
This study aimed to explore the protective effect of Shenfu on the hemodynamics and gut integrity in a porcine model of hemorrhagic shock. Hemorrhagic shock was induced in 32 domestic pigs with a rapid bleeding via the arterial sheath to a mean arterial pressure of 40 mm Hg within 10 min. Animals with hemorrhagic shock were then randomly assigned into the negative control group (n=8), receiving neither blood transfusion nor drug treatment; the blood transfusion group, in which animals were given blood transfusion alone; the saline group, in which animals were blood transfused and resuscitated with saline (3 mL/kg); and the Shenfu group, in which animals received blood transfusion and resuscitation with Shenfu (3 mL/kg). Blood tumor necrosis factor-alpha (TNF-É) and interleukin-6 were measured using ELISAs. Tissue levels of superoxide dismutase (SOD), malondialdehyde (MDA), Na+/K+-ATPase, Ca++ATPase, myeloperoxidase (MPO), and fatty acid binding protein 2 (FABP2) were determined using respective quantitation kits. Fluid resuscitation with Shenfu significantly improved HR, CI, and MAP of pig with hemorrhagic shock, which was accompanied with mitigation of tissue damages in intestinal epithelium. Blood TNF-É was reduced in the Shenfu group. Bcl-2 and cleaved caspase-3 expression in intestinal tissues were elevated and decreased, respectively, in pigs treated with Shenfu. Notably, treatment with Shenfu suppressed oxidative stress markers MDA, MPO, and FABP2 in the intestine. Oppositely, SOD, Na+/K+-ATPase and Ca++ATPase levels in intestinal tissues were promoted by Shenfu treatment. Shenfu demonstrates significant protective effect on the hemodynamics and gut epithelium of pigs with hemorrhagic shock.
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Medicamentos de Ervas Chinesas/farmacologia , Epitélio/efeitos dos fármacos , Choque Hemorrágico , Adenosina Trifosfatases , Animais , Modelos Animais de Doenças , Interleucina-6/sangue , Choque Hemorrágico/complicações , Choque Hemorrágico/tratamento farmacológico , Superóxido Dismutase , Suínos , Fator de Necrose Tumoral alfa/sangueRESUMO
BACKGROUND: Shenfu injection (SFI) is a traditional Chinese herbal medicine which has been clinically used for treatment of septic shock and cardiac shock. The aim of this study was to clarify effects of SFI on cerebral microcirculation and brain injury after hemorrhagic shock (HS). METHODS: Twenty-one domestic male Beijing Landrace pigs were randomly divided into three groups: SFI group (SFI, nâ=â8), saline group (SA, nâ=â8) or sham operation group (SO, nâ=â5). In the SFI group, animals were induced to HS by rapid bleeding to a mean arterial pressure of 40âmmHg within 10 minutes and maintained at 40±3âmmHg for 60 minutes. Volume resuscitation (shed blood and crystalloid) and SFI were given after 1 hour of HS. In the SA group, animals received the same dose of saline instead of SFI. In the SO group, the same surgical procedure was performed but without inducing HS and volume resuscitation. The cerebral microvascular flow index (MFI), nitric oxide synthase (NOS) expression, aquaporin-4 expression, interleukin-6, tumor necrosis factor-α (TNF-α) and ultrastructural of microvascular endothelia were measured. RESULTS: Compared with the SA group, SFI significantly improved cerebral MFI after HS. SFI up regulated cerebral endothelial NOS expression, but down regulated interleukin-6, TNF-α, inducible NOS and aquaporin-4 expression compared with the SA group. The cerebral microvascular endothelial injury and interstitial edema in the SFI group were lighter than those in the SA group. CONCLUSIONS: Combined application of SFI with volume resuscitation after HS can improve cerebral microcirculation and reduce brain injury.
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Lesões Encefálicas/tratamento farmacológico , Medicamentos de Ervas Chinesas/química , Microcirculação , Extratos Vegetais/administração & dosagem , Choque Hemorrágico/complicações , Animais , Lesões Encefálicas/etiologia , Lesões Encefálicas/metabolismo , Lesões Encefálicas/patologia , Citocinas/metabolismo , Masculino , SuínosRESUMO
Application of low concentrations of hydrogen peroxide (H2O2) is a relatively new and promising method to selectively suppress harmful cyanobacterial blooms, while minimizing effects on eukaryotic organisms. However, it is still unknown how nutrient limitation affects the sensitivity of cyanobacteria to H2O2. In this study, we compare effects of H2O2 on the microcystin-producing cyanobacterium Microcystis PCC 7806 under light-limited but nutrient-replete conditions, nitrogen (N) limitation and phosphorus (P) limitation. Microcystis was first grown in chemostats to acclimate to these different experimental conditions, and subsequently transferred to batch cultures where they were treated with a range of H2O2 concentrations (0-10 mg L-1) while exposed to high light (100 µmol photons m-2 s-1) or low light (15 µmol photons m-2 s-1). Our results show that, at low light, N- and P-limited Microcystis were less sensitive to H2O2 than light-limited but nutrient-replete Microcystis. A significantly higher expression of the genes encoding for anti-oxidative stress enzymes (2-cys-peroxiredoxin, thioredoxin A and type II peroxiredoxin) was observed prior to and after the H2O2 treatment for both N- and P-limited Microcystis, which may explain their increased resistance against H2O2. At high light, Microcystis was more sensitive to H2O2 than at low light, and differences in the decline of the photosynthetic yield between nutrient-replete and nutrient-limited Microcystis exposed to H2O2 were less pronounced. Leakage of microcystin was stronger and faster from nutrient-replete than from N- and P-limited Microcystis. Overall, this study provides insight in the sensitivity of harmful cyanobacteria to H2O2 under various environmental conditions.
Assuntos
Cianobactérias , Microcystis , Peróxido de Hidrogênio , Nutrientes , FotossínteseRESUMO
A phytoremediation project involving the large-scale cultivation of water hyacinths (Eichhornia crassipes (Mart.) Solms) was conducted in Lake Caohai (China) from May to November during 2011-2013 to remove pollutants and decrease eutrophication. Water hyacinths were cultivated in two areas of Lake Caohai, Neicaohai, and Waicaohai, which are connected and function as a relatively independent water body. The areas for macrophyte growth varied in size from 4.30 km2 in 2011 to 0.85 km2 (2012) and 1.15 km2 (2013). Compared with historical data from 2007, the concentrations of total phosphorus decreased significantly, while dissolved oxygen concentrations increased slightly. After plant cultivation in 2011, the average concentrations of total phosphorus, total dissolved phosphorus, and phosphate anions decreased from 0.54, 0.35, and 0.23 mg L-1 upstream (river estuaries) to 0.15, 0.13, and 0.08 mg L-1 downstream (Xiyuan Channel), respectively. The amount of phosphorus assimilated by the macrophytes (44.31 t) was more than 100% of the total removed phosphorus (40.93 t) from lake water when water hyacinths covered 40.9% of the area, which could indicate sedimentary phosphorus release. Our study showed the great potential of utilizing water hyacinth phytoremediation to remove phosphorus in eutrophic waters.