RESUMO
Choroideremia (CHM) is an X-linked progressive degeneration of the choroid and retina. 12% of unrelated male patients carry deletions of the partially cloned CHM gene. In Finland, there are more than 120 living CHM patients belonging to eight apparently unrelated pedigrees. Molecular deletions involving the CHM gene have been detected in three families. We have screened the remaining five families for point mutations. In one large family a single nucleotide (T) insertion into the donor splice site of exon C leads to two aberrantly spliced mRNAs both producing a premature stop codon. The mutation can be assayed easily by amplification and digestion with Msel. Our findings provide additional evidence for the pathogenetic role of CHM mutations and provide a diagnostic tool for one fifth of the world's known CHM patients.
Assuntos
Coroideremia/genética , Splicing de RNA/genética , Sequência de Aminoácidos , Sequência de Bases , DNA/genética , Análise Mutacional de DNA , Feminino , Finlândia , Humanos , Masculino , Dados de Sequência Molecular , Linhagem , Mutação Puntual , Reação em Cadeia da PolimeraseRESUMO
Mapping of the human MAOA gene to chromosomal region Xp21-p11 prompted our study of two affected males in a family previously reported to have Norrie disease resulting from a submicroscopic deletion in this chromosomal region. In this investigation we demonstrate in these cousins deletion of the MAOA gene, undetectable levels of MAO-A and MAO-B activities in their fibroblasts and platelets, respectively, loss of mRNA for MAO-A in fibroblasts, and substantial alterations in urinary catecholamine metabolites. The present study documents that a marked deficiency of MAO activity is compatible with life and that genes for MAO-A and MAO-B are near each other in this Xp chromosomal region. Some of the clinical features of these MAO deletion patients may help to identify X-linked MAO deficiency diseases in humans.
Assuntos
Deleção Cromossômica , Monoaminoxidase/deficiência , Aberrações dos Cromossomos Sexuais , Cromossomo X , Adolescente , Adulto , Plaquetas/enzimologia , Northern Blotting , Southern Blotting , Células Cultivadas , Criança , DNA/genética , DNA/isolamento & purificação , Feminino , Fibroblastos/enzimologia , Humanos , Isoenzimas/deficiência , Isoenzimas/genética , Isoenzimas/metabolismo , Masculino , Monoaminoxidase/genética , Monoaminoxidase/metabolismo , Linhagem , RNA/genética , RNA/isolamento & purificação , Valores de Referência , Pele/enzimologiaRESUMO
Usher syndrome type 3 is caused by mutations in the USH3A gene, which encodes the protein clarin-1. Clarin-1 is a member of the tetraspanin superfamily (TM4SF) of transmembrane proteins, expressed in the organ of Corti and spiral ganglion cells of the mouse ear. We have examined whether the AAV-mediated anti-clarin ribozyme delivery causes apoptotic cell death in vivo in the organ of Corti. We used an AAV-2 vector delivered hammerhead ribozyme, AAV-CBA-Rz, which specifically recognizes and cleaves wild type mouse clarin-1 mRNA. Cochleae of CD-1 mice were injected either with 1mul of the AAV-CBA-Rz, or control AAV vectors containing the green fluorescent protein (GFP) marker gene (AAV-CBA-GFP). Additional controls were performed with saline only. At one-week and one-month post-injection, the animals were sacrificed and the cochleae were studied by histology and fluorescence imaging. Mice injected with AAV-CBA-GFP displayed GFP reporter expression of varying fluorescence intensity throughout the length of the cochlea in the outer and inner hair cells and stria vascularis, and to a lesser extent, in vestibular epithelial cells. GFP expression was not detectable in the spiral ganglion. The pro-apoptotic effect of AAV-CBA-delivered anti-clarin-1 ribozymes was evaluated by TUNEL-staining. We observed in the AAV-CBA-Rz, AAV-CBA-GFP and saline control groups apoptotic nuclei in the outer and inner hair cells and in the stria vascularis one week after the microinjection. The vestibular epithelium was also observed to contain apoptotic cells. No TUNEL-positive spiral ganglion neurons were detected. After one-month post-injection, the AAV-CBA-Rz-injected group had significantly more apoptotic outer and inner hair cells and cells of the stria vascularis than the AAV-CBA-GFP group. In this study, we demonstrate that AAV-CBA mediated clarin-1 ribozyme may induce apoptosis of the cochlear hair cells and cells of the stria vascularis. Surprisingly, we did not observe apoptosis in spiral ganglion cells, which should also be susceptible to clarin-1 mRNA cleavage. This result may be due to the injection technique, the promoter used, or tropism of the AAV serotype 2 viral vector. These results suggest the role of apoptosis in the progression of USH3A hearing loss warrants further evaluation.
Assuntos
Apoptose , Cóclea/patologia , Dependovirus/genética , Técnicas de Transferência de Genes , Vetores Genéticos , Proteínas de Membrana/metabolismo , RNA Catalítico/metabolismo , Síndromes de Usher/patologia , Animais , Cóclea/metabolismo , Genes Reporter , Proteínas de Fluorescência Verde , Células Ciliadas Auditivas/metabolismo , Células Ciliadas Auditivas/patologia , Marcação In Situ das Extremidades Cortadas , Masculino , Proteínas de Membrana/genética , Camundongos , Microscopia de Fluorescência , RNA Mensageiro/metabolismo , Estria Vascular/metabolismo , Estria Vascular/patologia , Fatores de Tempo , Síndromes de Usher/genética , Síndromes de Usher/metabolismoRESUMO
BACKGROUND: X linked cone-rod dystrophy (CORDX) is a recessive retinal disease characterised by progressive dysfunction of photoreceptors. It is genetically heterogeneous, showing linkage to three X chromosomal loci. CORDX1 is caused by mutations in the RPGR gene (Xp21.1), CORDX2 is located on Xq27.2-28, and we recently localised CORDX3 to Xp11.4-q13.1. We aimed to identify the causative gene behind the CORDX3 phenotype. METHODS: All 48 exons of the CACNA1F gene were screened for mutations by DNA sequencing. RNA from cultured lymphoblasts and peripheral blood activated T lymphocytes was analysed by RT-PCR and sequencing. RESULTS: A novel CACNA1F mutation, IVS28-1 GCGTC>TGG, in the splice acceptor site of intron 28 was identified. Messenger RNA studies indicated that the identified mutation leads to altered splicing of the CACNA1F transcript. Aberrant splice variants are predicted to result in premature termination and deletions of the encoded protein, Ca(v)1.4 alpha1 subunit. CONCLUSION: CACNA1F mutations cause the retinal disorder, incomplete congenital stationary night blindness (CSNB2), although mutations have also been detected in patients with divergent diagnoses. Our results indicate that yet another phenotype, CORDX3, is caused by a mutation in CACNA1F. Clinically, CORDX3 shares some features with CSNB2 but is distinguishable from CSNB2 in that it is progressive, can begin in adulthood, has no nystagmus or hyperopic refraction, has only low grade astigmatism, and in dark adaptation lacks cone threshold and has small or no elevation of rod threshold. Considering all features, CORDX3 is more similar to other X chromosomal cone-rod dystrophies than to CSNB2.
Assuntos
Canais de Cálcio Tipo L/genética , Doenças Genéticas Ligadas ao Cromossomo X/genética , Mutação/genética , Retinose Pigmentar/genética , Adulto , Estudos de Casos e Controles , Criança , Análise Mutacional de DNA , Éxons/genética , Feminino , Humanos , Masculino , RNA Mensageiro/genética , RNA Mensageiro/metabolismoRESUMO
AIM: To perform genealogical and clinical studies in Finnish families with X linked ocular albinism (OA1), including characterisation of the potential misrouting of optic fibres by evaluating visual evoked magnetic fields (VEFs), and to determine the mutation behind the disease. METHODS: Three families with OA1 were clinically examined. VEFs were measured in two affected males and in one female carrier to characterise the cortical activation pattern after monocular visual stimulation. The neuronal sources of the VEFs were modelled with equivalent current dipoles (ECDs) in a spherical head model. All coding exons of the OA1 gene were screened for mutations by single strand conformation analysis and direct polymerase chain reaction sequencing. RESULTS: Genealogical studies revealed that the three families were all related. The affected males had foveal hypoplasia with reduced visual acuity varying from 20/200 to 20/50, variable nystagmus, iris transillumination, and hypopigmentation of the retinal pigment epithelium. The ECD locations corresponding to the VEFs revealed abnormal crossing of the optic fibres in both affected males, but not in the carrier female. A novel point mutation, leading to a STOP codon, was identified in the fifth exon of the OA1 gene. CONCLUSIONS: The data indicate that the novel mutation 640C>T in the OA1 gene is the primary cause of the eye disease in the family studied. VEFs with ECD analysis was successfully used to demonstrate abnormal crossing of the optic fibres.
Assuntos
Albinismo Ocular/genética , Proteínas do Olho/genética , Olho/inervação , Doenças Genéticas Ligadas ao Cromossomo X/genética , Glicoproteínas de Membrana/genética , Fibras Nervosas , Nervo Óptico/anormalidades , Adulto , Albinismo Ocular/patologia , Saúde da Família , Feminino , Doenças Genéticas Ligadas ao Cromossomo X/patologia , Humanos , Magnetoencefalografia/métodos , Masculino , Pessoa de Meia-Idade , Linhagem , Mutação Puntual/genética , Campos Visuais/fisiologiaRESUMO
PURPOSE: Autosomal recessive corneal plana (RCP) is a rare corneal anomaly with unknown pathogenesis and a high incidence in Finland. The aim was to examine corneal sensitivity and the morphology of different corneal layers and subbasal nerves in RCP patients. METHODS: Three patients with a diagnosed autosomal recessive cornea plana were examined. Corneal sensitivity to different modalities of stimulation was tested in four corneas using noncontact esthesiometry. Tissue morphology of three corneas was evaluated, and in two corneas thickness of corneal layers was measured using in vivo confocal microscopy. RESULTS: Corneas of RCP patients appear to have mechanosensory, polymodal, and cold-sensitive nerve terminals. RCP patients had normal sensation thresholds for chemical, heat, and cold stimulation but a high threshold for mechanical stimulation. Their capacity to discriminate increasing intensities of stimulus was reduced, except for cold stimuli. Thickness of the epithelial layer was reduced, whereas total corneal and stromal thicknesses were slightly reduced or close to normal values. In all cases Bowman's layer was absent. Subbasal nerves had abnormal branching patterns. The arrangement of anterior keratocytes was altered, showing clustered and irregularly shaped nuclei. Increased backscattering of light in confocal microscopy through focusing (CMTF) profiles was observed throughout the stroma. Epithelial and endothelial cells appeared to be regular in shape. CONCLUSIONS: The present study revealed qualitative and quantitative alterations in corneal sensitivity, cellular morphology, and the thickness of corneal layers in RCP patients.
Assuntos
Córnea/inervação , Distrofias Hereditárias da Córnea/patologia , Distrofias Hereditárias da Córnea/fisiopatologia , Nervo Oftálmico/fisiopatologia , Sensação , Córnea/patologia , Substância Própria/patologia , Epitélio Corneano/patologia , Feminino , Humanos , Masculino , Microscopia Confocal , Pessoa de Meia-Idade , Fibras Nervosas/patologia , ReflexoRESUMO
Audiometric features, evaluated by serial pure tone audiometry and speech recognition tests (n = 31), were analysed in 59 Finnish Usher syndrome type III patients (USH3) with Finmajor/Finmajor (n = 55) and Finmajor/Finminor (n = 4) USH3A mutations. These patients showed a highly variable type and degree of progressive sensorineural hearing impairment: from normal to moderate USH2A-like hearing impairment at young ages to profound or even USH1B-like hearing impairment at more advanced ages. Compound heterozygous patients generally showed a milder phenotype. The highest progression was seen during the first two decades of life, gradually slowing down with further ageing. This type of non-linear progression may be unique amongst the Usher syndromes. Speech recognition started to deteriorate at highly variable ages. In some patients, it jeopardised normal speech and language development, whereas in others it was still remarkably good at advanced ages.
Assuntos
Audiometria de Tons Puros/métodos , Audiometria da Fala/métodos , Limiar Auditivo/fisiologia , Perda Auditiva Neurossensorial/genética , Perda Auditiva Neurossensorial/fisiopatologia , Adolescente , Adulto , Fatores Etários , Criança , Pré-Escolar , Progressão da Doença , Feminino , Finlândia , Genótipo , Humanos , Estudos Longitudinais , Masculino , Proteínas de Membrana/genética , Pessoa de Meia-Idade , Mutação , Fenótipo , Análise de Regressão , Retinose Pigmentar/genética , Retinose Pigmentar/fisiopatologia , Percepção da Fala , SíndromeRESUMO
Genetic counselling endeavours to be nondirective. However, the availability of prenatal diagnosis may direct clients towards accepting and using these methods. It is time to investigate the attitudes of clients in order to monitor the psychological and social effects of new genetic techniques. As prenatal diagnosis was possible for choroideremia (C), but not for retinitis pigmentosa (RP) in 1988-89, we used a questionnaire to compare the attitudes of C and RP patients, their relatives and C carriers to prenatal diagnosis. The response rate was low (35%) and no significant differences between RP and C groups came to light. However, C carriers accepted prenatal diagnosis and also selective abortion more easily, but, on the other hand, they showed more uncertainty than did the other groups. This indicates that the availability of prenatal diagnosis may confuse those concerned. In general, about 60% of all the respondents had a positive attitude to the prenatal diagnosis of RP or choroideremia, though only about 30% would use if for abortion. Over 80% of all the respondents wanted to know the opinion of the genetic counsellor.
Assuntos
Aborto Terapêutico/psicologia , Atitude Frente a Saúde , Coroideremia/psicologia , Doenças Genéticas Inatas , Diagnóstico Pré-Natal/psicologia , Retinose Pigmentar/psicologia , Adolescente , Adulto , Coroideremia/diagnóstico , Compreensão , Feminino , Finlândia , Triagem de Portadores Genéticos , Aconselhamento Genético , Humanos , Masculino , Pessoa de Meia-Idade , Retinose Pigmentar/diagnóstico , Inquéritos e Questionários , IncertezaRESUMO
We report a previously undescribed autosomal reciprocal translocation, t(1;8)(q41;q23.1). It segregates in three families whose common origin lies at least 11 generations back. No examples of unbalanced karyotypes were encountered. Moreover, there was no circumstantial evidence that such live births had occurred during earlier generations. Couples in which one spouse was a translocation carrier were compared to related couples with normal karyotypes. The 15 carrier families had significantly more spontaneous abortions (32%) than the 22 normal couples (10%), irrespective of the sex of the carrier parent. However, the mean number of children was equal in both groups (2.0 and 2.4). Carrier families produced 17 children with a balanced translocation and seven with a normal karyotype. This deviates significantly (P = .04) from the expected 1:1 ratio. We conclude that this malsegregation helps to maintain the translocation in the population. These results show that empirically derived 1:1 segregation ratios previously reported in series that combine many different translocations do not apply to all individual translocations.
Assuntos
Aborto Habitual/genética , Cromossomos Humanos 1-3 , Cromossomos Humanos 6-12 e X , Translocação Genética , Bandeamento Cromossômico , Feminino , Finlândia , Aconselhamento Genético , Heterozigoto , Humanos , Recém-Nascido , Cariotipagem , Masculino , Linhagem , GravidezRESUMO
We report linkage studies in 18 choroideremia (TCD) families using four closely linked polymorphic markers. Probe pZ11, which is known to be deleted in several unrelated patients with TCD, showed no recombinations (zeta max 15.63 at theta = 0.00). In contrast, one recombination was observed with DXS367, which is also physically very close to TCD. Loci DXS95 and DXYS69 each showed more than one recombination with TCD. Moreover, these analyses revealed a double crossover between TCD and DXYS1, changing the previously reported very close linkage to a recombination fraction of 0.04 with a lod score of 9.93. Multipoint linkage analysis placed TCD proximal to DXS95-DXYS69 and very close to DXS367-pZ11 with almost identical multipoint lod score maxima either proximal to DXS367 (zeta max = 23.43) or proximal to pZ11 (zeta max = 23.36). These results provide a refined linkage map around TCD and will also be useful in DNA diagnostics of the disease.
Assuntos
Coroideremia/genética , Ligação Genética , Sondas de DNA , Feminino , Humanos , Escore Lod , Masculino , Linhagem , Polimorfismo de Fragmento de Restrição , Cromossomo XRESUMO
Usher syndrome type 3 (USH3; MIM 276902) is an autosomal recessive disorder associated with progressive hearing loss and retinal degeneration. We recently refined the localization of USH3 to a 1-cM genetic interval between markers D3S1299 and D3S3625. We have now constructed a bacterial artificial chromosome contig over the region. Novel polymorphic markers were generated and physically fine-mapped, allowing further narrowing of the critical interval to a 250-kb genomic fragment. Of seven ESTs mapping to the initial critical region, WI-11588 and SHGC-133 represent the human SIAH2 gene, which was excluded as a candidate for USH3 by sequencing and subsequently, by its position. KIAA0001 and D3S3882 derive from the transcript of a putative G-protein-coupled receptor gene that was excluded as a candidate by sequencing of patient DNA. These data provide a basis for the sequencing and final characterization of the USH3 region and isolation of the disease gene.
Assuntos
Cromossomos Humanos Par 3/genética , Perda Auditiva Neurossensorial/genética , Degeneração Retiniana/genética , Doenças Vestibulares/genética , Cromossomos Bacterianos , Etiquetas de Sequências Expressas , Finlândia , Genes Recessivos , Vetores Genéticos , Humanos , Desequilíbrio de Ligação , Dados de Sequência Molecular , SíndromeRESUMO
Familial juvenile nephronophthisis (NPH) is an autosomal recessive tubulointerstitial kidney disease associated with formation of medullary and corticomedullary cysts. It progresses to end stage renal failure and its biochemical defect is unknown. An NPH locus has been assigned to a 2 cM interval on chromosome 2q13 by linkage studies. Homozygous deletions of approximately 250 kb have been detected in 80% of familial cases and 65% of sporadic cases and a common mutation mechanism has been suggested. We examined 14 Finnish families for the presence or absence of a deletion. After detecting a deletion in 12 patients belonging to nine families, we studied a possible founder effect by haplotype analysis using markers D2S340, D2S1889, and D2S1893. No common ancestral disease associated haplotype was found suggesting no founder effect. Results of pairwise linkage analyses were suggestive of linkage in the nine families with a deletion (lod scores of 1.39-3.89 at a recombination fraction of 0). Negative lod scores were obtained in the five families without a deletion suggesting that the disease locus in these families lies elsewhere. The end stage renal disease occurred at a more advanced age in patients without a deletion compared to patients with a deletion, indicating a phenotypic difference between these two groups.
Assuntos
Cromossomos Humanos Par 2 , Efeito Fundador , Mutação , Nefrite Intersticial/genética , Rim Policístico Autossômico Recessivo/genética , Adulto , Criança , Feminino , Finlândia , Deleção de Genes , Haplótipos , Humanos , Desequilíbrio de Ligação , Masculino , LinhagemRESUMO
Carrier determination and prenatal diagnosis in Norrie disease (ND) has so far not been reported. We describe a kindred with 4 members affected by ND in which a deletion comprising gene locus DXS7 on the short arm of the X chromosome defined by probe L1.28 causes the disorder. This allowed us to predict via chorion villus biopsy that a male foetus of a carrier woman is unaffected.
Assuntos
Cegueira/congênito , Aberrações Cromossômicas/diagnóstico , Deficiência Intelectual/diagnóstico , Cegueira/diagnóstico , Cegueira/genética , Deleção Cromossômica , Transtornos Cromossômicos , Clonagem Molecular , Enzimas de Restrição do DNA , Feminino , Triagem de Portadores Genéticos , Humanos , Deficiência Intelectual/genética , Linhagem , Gravidez , Diagnóstico Pré-NatalRESUMO
Linkage studies using restriction fragment length polymorphisms were conducted in the X-linked disorder, choroideremia, designated TCD for Progressive Tapeto-Choroidal Dystrophy. Previously demonstrated close linkage with locus DXYS1 was confirmed (lod 11.44 at 0 recombination distance). In addition, locus DXYS12 was found to be closely linked with TCD (lod 3.31 at 0 recombination distance). The disease mainly occurs in three large kindreds in remote Northern Finland. While formal genealogical proof is lacking, all presently living (more than 80 affected males and 120 carrier females) probably originate from a common founder couple born in 1644 and 1646, twelve generations ago. All 36 patients and 48 carriers tested from the three kindreds had the same haplotype (TCD/DXYS1, 11kb/DXYS12, 1.6kb). Given that at least 105 female meioses transmitting TCD have occurred since 1650 in these kindreds, extremely close linkage between TCD, DXYS1 and DXYS12 is suggested. The above haplotype is a very useful diagnostic tool in these TCD families. We suggest that our historical-genealogical approach to linkage analysis may be possible elsewhere in similar isolated populations.
Assuntos
Corioide , Ligação Genética , Doenças da Úvea/genética , Cromossomo X , Alelos , Métodos Epidemiológicos , Feminino , Finlândia , Frequência do Gene , Humanos , Masculino , Linhagem , Polimorfismo de Fragmento de Restrição , Gravidez , Diagnóstico Pré-Natal , Doenças da Úvea/diagnósticoRESUMO
A three generation family with X linked myotubular myopathy (MTM1) was studied with several polymorphic markers from the distal long arm of the X chromosome. A recombination between the disease gene and four markers (loci DXS52, DXS134, DXS15, F8C) from the Xq28 cluster was detected. A new polymorphic marker (U6.2) defining the locus DXS304 in the Xq27-28 region proximal to the Xq28 cluster did not show any recombination with MTM1. These results suggest the following order of loci in distal Xq: cen-DXS42-DXS105-(DXS304, MTM1)-(DXS52, DXS134, DXS15, F8C)-tel.
Assuntos
Núcleo Celular/ultraestrutura , Aberrações Cromossômicas , DNA/análise , Ligação Genética , Atrofia Muscular/genética , Cromossomo X , Marcadores Genéticos/análise , Humanos , Recém-Nascido , Masculino , Família Multigênica , Atrofia Muscular/patologia , Linhagem , Polimorfismo de Fragmento de Restrição , Recombinação GenéticaRESUMO
A locus for Usher syndrome type III (USH3; MIM No. 276902) was recently assigned to a 5-cM region on chromosome 3q. We constructed a yeast artificial chromosome contig that allowed us to position novel polymorphisms in the region. These were typed in a total of 32 pedigrees from a geographically isolated Finnish founder population in which a putative single ancestral USH3 mutation segregates. A multipoint linkage analysis assigned USH3 to a 4-cM region between D3S1555 and a novel marker D3S3625. By analysis of linkage disequilibrium and historical recombinations in 77 USH3 chromosomes, the location of the Finnish USH3 mutation could be narrowed to an approximately 1-cM interval between the markers D3S1299 and D3S3625. A gene for profilin-2 (PFN2) was mapped in the vicinity and excluded as a candidate for USH3 by sequencing. The putative mouse homolog of PFN2 was mapped to mouse chromosome 3, thus suggesting a localization for the mouse homolog of USH3.
Assuntos
Cromossomos Humanos Par 3/genética , Proteínas Contráteis , Perda Auditiva Neurossensorial/genética , Camundongos/genética , Retinose Pigmentar/genética , Doenças Vestibulares/genética , Alelos , Animais , Mapeamento Cromossômico , Cromossomos Artificiais de Levedura/genética , Finlândia/epidemiologia , Efeito Fundador , Haplótipos/genética , Perda Auditiva Neurossensorial/classificação , Perda Auditiva Neurossensorial/epidemiologia , Humanos , Desequilíbrio de Ligação , Escore Lod , Proteínas dos Microfilamentos/genética , Profilinas , Retinose Pigmentar/classificação , Retinose Pigmentar/epidemiologia , Homologia de Sequência , Especificidade da Espécie , Síndrome , Doenças Vestibulares/classificação , Doenças Vestibulares/epidemiologiaRESUMO
Usher syndrome (USH) refers to genetically and clinically heterogeneous autosomal recessive disorders with combined visual and hearing loss. Type I (USH1) is characterized by a congenital, severe to profound hearing loss and absent vestibular function; in type II (USH2) the hearing loss is congenital and moderate to severe, and the vestibular function is normal. Progressive pigmentary retinopathy (PPR) is present in both types. A third type (USH3) differing from USH2 by the progressive nature of its hearing loss has been suggested. USH3 has previously been estimated to comprise 2% of all USH. However, based on clinical criteria, in Finland 42% of USH patients have progressive hearing loss suggesting enrichment of an USH3 gene. We excluded the four previously mapped USH regions as the site of the USH3 disease locus. Systematic search for USH3 by genetic linkage analyses in 10 multiple affected families using polymorphic microsatellite markers revealed significant linkage with markers mapping to chromosome 3q. Pairwise lod scores at zero recombination distance were 7.87 for D3S1308, and 11.29 for D3S1299, incorporating the observed linkage disequilibrium. Conventional multipoint linkage analysis gave a maximum lod score of 9.88 at D3S1299 assigning USH3 to the 5 cM interval between markers D3S1555 and D3S1279 in 3q21-25.(ABSTRACT TRUNCATED AT 250 WORDS)