Ionic liquid-based carbon dots as highly biocompatible and sensitive fluorescent probe for the determination of vitamin P in fruit samples.

Vitamin P (VP) known as rutin is one of the common flavonoids, which widely exists in fruits and vegetables and often used as a dietary additive. The rapid and accurate detection of VP in food matrices is critical for evaluating food quality and guiding diet. Herein, a rapid, accurate, and selective detection scheme for VP in fruit samples was proposed for the first time using ionic liquid-based carbon dots (IL-CDs). The synthesized IL-CDs exhibited great biocompatibility and excellent optical properties including high fluorescence intensity, high quantum yield, and good fluorescence stability. Through an internal filtering effect (IFE), VP could greatly reduce the fluorescence of these CDs. In the present study, this probe demonstrated good sensitivity and excellent selectivity toward VP with a low detection limit of 60.0 nmol/L. Moreover, this approach was effectively applied to detect VP in food samples with a recovery range of 97 % to 119 %. More interestingly, the results of cell imaging suggested that IL-CDs were expected to be promising material for bioimaging.

Targeting Nrf2 with 3 H-1,2-dithiole-3-thione to moderate OXPHOS-driven oxidative stress attenuates IL-17A-induced psoriasis.

Psoriasis, a chronic autoimmune disease characterized by the hyperproliferation of keratinocytes in the epidermis and parakeratosis, significantly impacts quality of life. Interleukin (IL)- 17A dominates the pathogenesis of psoriasis and facilitates reactive oxygen species (ROS) accumulation, which exacerbates local psoriatic lesions. Biologic treatment provides remarkable clinical efficacy, but its high cost and unignorable side effects limit its applications. 3 H-1,2-Dithiole-3-thione (D3T) possesses compelling antioxidative capacities against several diseases through the nuclear factor erythroid 2-related factor 2 (Nrf2) cascade. Hence, we aimed to evaluate the effect and mechanism of D3T in psoriasis. We found that D3T attenuates skin thickening and scaling by inhibiting IL-17A-secreting γδT cells in imiquimod (IMQ)-induced psoriatic mice. Interleukin-17A markedly enhanced IL-6 and IL-8 expression, lipid peroxidation, the contents of nitric oxide and hydrogen peroxide, oxidative phosphorylation and the MAPK/NF-κB pathways in keratinocytes. IL-17A also inhibited the Nrf2-NQO1-HO-1 axis and the activities of superoxide dismutase and glutathione peroxidase. D3T significantly reversed these parameters in IL-17A-treated keratinocytes. ML-385, a Nrf2 neutralizer, failed to improve D3T-induced anti-inflammatory and antioxidative effects in IL-17A-treated keratinocytes. We conclude that targeting Nrf2 with D3T to diminish oxidative and inflammatory damage in keratinocytes may attenuate psoriasis.