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1.
J Dairy Res ; 91(1): 108-115, 2024 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-38494756

RESUMO

This research paper addresses the hypotheses that Kluyveromyces marxianus can be cultured with good alcohol production on different whey-derived matrices, and that the fermented product can be used in order to develop alcoholic beverages with acceptable sensory characteristics by mixtures with yeast-fermented fruit-based matrices. Growth and fermentative characteristics of Kluyveromyces marxianus LFIQK1 in different whey-derived matrices were explored by culturing (24 h, 30°C) on reconstituted whey, demineralized whey, heat-treated whey and milk permeate media. High lactose consumption, ethanol production and yield were observed. Reconstituted whey matrix was selected for mixing with orange or strawberry juices fermented using Saccharomyces cerevisiae to obtain alcoholic beverages (W-OR and W-ST, respectively). Consumer evaluation of beverages was performed using acceptability and Check-All-That-Apply (CATA) questions. Good acceptance was observed, significantly higher for W-ST than for W-OR. CATA questions gave information about organoleptic characteristics of beverages. Penalty analysis showed W-R and W-ST were positively associated with smooth/refreshing and fruity/natural, respectively. Liking was represented, accordingly with penalty analysis, by natural/refreshing. A novel alternative for utilization of whey and whey-related matrices by alcoholic beverages production with natural ingredients is presented.


Assuntos
Bebidas Alcoólicas , Fermentação , Sucos de Frutas e Vegetais , Kluyveromyces , Soro do Leite , Kluyveromyces/metabolismo , Soro do Leite/química , Bebidas Alcoólicas/análise , Sucos de Frutas e Vegetais/análise , Etanol/metabolismo , Saccharomyces cerevisiae/metabolismo , Paladar , Humanos
2.
Small ; 19(33): e2301247, 2023 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-37086132

RESUMO

Producing hydrogen via electrochemical water splitting with minimum environmental harm can help resolve the energy crisis in a sustainable way. Here, this work fabricates the pure nickel nanopyramid arrays (NNAs) with dense high-index crystalline steps as the cata electrode via a screw dislocation-dominated growth kinetic for long-term durable and large current density hydrogen evolution reaction. Such a monolithic NNAs electrode offers an ultralow overpotential of 469 mV at a current density of 5000 mA cm-2 in 1.0 m KOH electrolyte and shows a high stability up to 7000 h at a current density of 1000 mA cm-2 , which outperforms the reported catas and even the commercial platinum cata for long-term services under high current densities. Its unique structure can substantially stabilize the high-density surface crystalline steps on the catalytic electrode, which significantly elevates the catalytic activity and durability of nickel in an alkaline medium. In a typical commercial hydrogen gas generator, the total energy conversion rate of NNAs reaches 84.5% of that of a commercial Pt/Ti cata during a 60-day test of hydrogen production. This work approach can provide insights into the development of industry-compatible long-term durable, and high-performance non-noble metal catas for various applications.

3.
J Food Sci Technol ; 60(7): 1981-1991, 2023 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-37206424

RESUMO

The objective was to optimize the phenolic compounds extraction from cocoa shells using the simplex-centroid design with a mixture of solvents (water, methanol, and acetone) as its components, to prove the presence of these compounds and antioxidant activity. Also, the development of dairy products, such as milk beverages and dairy desserts, with bioactive compounds, through the replacement of cocoa powder by cocoa shell was studied and evaluated sensorially. The extraction optimization indicated that a solvent with 56.44% water, 23.77% methanol, and 19.80% acetone are ideal for maximizing the phenolic compounds. In addition, the cocoa shell showed a high antioxidant activity by the methods ß-carotene/linoleic acid, FRAP, and phosphomolybdenum complex. The Check-All-That-Apply, Cochran's Q test, contingency analysis, and hierarchical cluster analysis allowed description characteristics of the dairy products and showed sensory differences between formulations with 100% cocoa shell and others. Both dairy products had good sensory acceptance in all attributes evaluated (appearance, flavor, texture, and overall impression), and their scores did not differ statistically by Tukey's test (p > 0.05). Thus, the cocoa shell is shown as an alternative substitute ingredient to be used in the dairy industry.

4.
Appetite ; 170: 105887, 2022 03 01.
Artigo em Inglês | MEDLINE | ID: mdl-34953971

RESUMO

Consumption of pulses plays a key role in achieving a healthier and more sustainable diet. Despite the increased availability of pulse-based foods on the French market, pulse consumption in France remains below recommended levels. The objective of this study was to capture French consumers' mental representations of various pulse-based foods with different levels of processing, using a Check-All-That-Apply (CATA) questionnaire with food images. We used CATA questionnaires, in two separate studies, to capture two aspects: consumers' beliefs about the products presented in the study, and the characteristics of those products, according to consumers. In Study 1, twelve images of pulses were presented (unprocessed, processed, and ready-to-eat) to investigate whether and how consumers differentiate between pulse-based products with different recipes and levels of processing. In Study 2, parallel CATA questionnaires were used: one with images of pulse-based products, and one with images of vegetables. We wanted to discover whether the factors underlying different representations for pulse-based products were specific to pulses, or were also observed with another family of food products. Results showed that products were differentiated more by their level of processing than by their main ingredient. Participants had more positive attitudes toward pulse products that were unprocessed, processed, or "new ready-to-eat", than toward other ready-to-eat pulse products (whether canned or frozen). Our results also highlighted that, in consumers' mental representations of pulse-based products, packaging format is a negligible factor in comparison with the nature of the product itself.


Assuntos
Preferências Alimentares , Verduras , Comportamento do Consumidor , Dieta , França , Humanos , Inquéritos e Questionários
5.
Food Qual Prefer ; 96: 104437, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-34720454

RESUMO

Restrictions adopted by many countries in 2020 due to Covid-19 pandemic had severe consequences on the management of sensory and consumer testing that strengthened the tendency to move data collection out of the laboratory. Remote sensory testing, organized at the assessor's home or workplace and carried out under the live online supervision of the panel leader, represents a trade-off between adequate control and the convenience of conducting testing out of the lab. The Italian Sensory Science Society developed the "Remote sensory testing" research project aimed at testing the effectiveness and validity of the sensory tests conducted remotely through a comparison with evaluations in a classical laboratory setting. Guidelines were developed to assist panel leaders in setting up and controlling the evaluation sessions in remote testing conditions. Different methods were considered: triangle and tetrad tests, Descriptive Analysis and Temporal Dominance of Sensations tests, all of which involved trained panels, and Check-All-That-Apply and hedonic tests with consumers. Remote sensory testing provided similar results to the lab testing in all the cases, with the exception of the tetrad test run at work. Findings suggest that remote sensory testing, if conducted in strict compliance with specifically developed sensory protocols, is a promising alternative to laboratory tests that can be applied with both trained assessors and consumers even beyond the global pandemic.

6.
Molecules ; 27(24)2022 Dec 12.
Artigo em Inglês | MEDLINE | ID: mdl-36557951

RESUMO

To elucidate the effects of the different terroir on wine aroma in six sub-regions of Eastern Foothills of Helan Mountain in Ningxia, a premium wine-producing region in China, 71 Cabernet Sauvignon wines were investigated by gas chromatography-mass spectrometry (GC-MS), check-all-that-apply (CATA), and quantitative descriptive analysis (QDA). The bidirectional orthogonal partial least squares-discriminant analysis (O2PLS-DA) results showed that the Cabernet Sauvignon dry red wines from Xixia (XX) and Yongning (YN) had similar volatile profiles due to their geographical proximity and were characterized by higher concentrations of esters, higher alcohols, and volatile phenols because the similar aromatic profiles were detected in their dry red wines. Shizuishan (SZS) and Hongsipu (HSP) wines showed clear differences compared to the wines of the other four sub-regions, being mainly characterized by relatively higher phenolic aldehydes and volatile phenols. The concentrations of methoxypyrazines and norisoprenoids varied mainly depending on the climate diversity of the sub-regions. The highest 3-isobutyl-2-methoxypyrazine (IBMP) concentration was presented in the Helan (HL) wines. The Qingtongxia (QTX) wines have the highest ß-damascenone, which might be influenced by the fact that QTX has the lowest effective accumulated temperature and the highest sunshine duration among the five sub-regions. Esters including ethyl octanoate, ethyl decanoate, ethyl butanoate, ethyl hexanoate, and isoamyl acetate were the highest in HL. Additionally, the herbaceous, black berry, and red berry notes in HL and QTX were the most outstanding.


Assuntos
Vitis , Compostos Orgânicos Voláteis , Cromatografia Gasosa-Espectrometria de Massas/métodos , Odorantes/análise , Bebidas Alcoólicas/análise , Fenóis/análise , Ésteres/análise , China , Vitis/química , Compostos Orgânicos Voláteis/análise
7.
Antimicrob Agents Chemother ; 65(9): e0060221, 2021 08 17.
Artigo em Inglês | MEDLINE | ID: mdl-34125594

RESUMO

Remdesivir (RDV; GS-5734, Veklury), the first FDA-approved antiviral to treat COVID-19, is a single-diastereomer monophosphoramidate prodrug of an adenosine analogue. RDV is taken up in the target cells and metabolized in multiple steps to form the active nucleoside triphosphate (TP) (GS-443902), which, in turn, acts as a potent and selective inhibitor of multiple viral RNA polymerases. In this report, we profiled the key enzymes involved in the RDV metabolic pathway with multiple parallel approaches: (i) bioinformatic analysis of nucleoside/nucleotide metabolic enzyme mRNA expression using public human tissue and lung single-cell bulk mRNA sequence (RNA-seq) data sets, (ii) protein and mRNA quantification of enzymes in human lung tissue and primary lung cells, (iii) biochemical studies on the catalytic rate of key enzymes, (iv) effects of specific enzyme inhibitors on the GS-443902 formation, and (v) the effects of these inhibitors on RDV antiviral activity against SARS-CoV-2 in cell culture. Our data collectively demonstrated that carboxylesterase 1 (CES1) and cathepsin A (CatA) are enzymes involved in hydrolyzing RDV to its alanine intermediate MetX, which is further hydrolyzed to the monophosphate form by histidine triad nucleotide-binding protein 1 (HINT1). The monophosphate is then consecutively phosphorylated to diphosphate and triphosphate by cellular phosphotransferases. Our data support the hypothesis that the unique properties of RDV prodrug not only allow lung-specific accumulation critical for the treatment of respiratory viral infection such as COVID-19 but also enable efficient intracellular metabolism of RDV and its MetX to monophosphate and successive phosphorylation to form the active TP in disease-relevant cells.


Assuntos
Tratamento Farmacológico da COVID-19 , SARS-CoV-2 , Monofosfato de Adenosina/análogos & derivados , Alanina/análogos & derivados , Antivirais/farmacologia , Humanos , Pulmão , Proteínas do Tecido Nervoso
8.
J Sci Food Agric ; 101(8): 3298-3307, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33222200

RESUMO

BACKGROUND: External preference mapping is a powerful tool to explain consumer preference or rejection. Combining the technique for order of preference by similarity to ideal solution (TOPSIS) multicriteria analysis with rapid descriptive techniques can improve preference map (PREFMAP) results. This study was conducted to compare the PREFMAPs generated with rapid descriptive flash profile (FP), check-all-that-apply (CATA), and Napping® versus PREFMAPs constructed with FP-TOPSIS, CATA-TOPSIS, and Napping-TOPSIS. RESULTS: Only 38.46%, 63.66%, and 42% of sensory attributes initially generated by FP, CATA, and Napping techniques respectively were considered for the determination of their weight W and allocation as positive or negative in the TOPSIS technique. The PREFMAPs constructed with FP-TOPSIS, CATA-TOPSIS, and Napping-TOPSIS presented a better explanation of the preference and rejection than the PREFMAPs directly generated with rapid sensory techniques. The results of the multiple factor analysis and coefficient Rv indicated similarities in the sensory vocabularies used after the TOPSIS technique. CONCLUSION: The combination of the TOPSIS technique with rapid sensory techniques is a reliable alternative for the construction of PREFMAPs in order to identify the sensory attributes responsible for preference and rejection of food products. © 2020 Society of Chemical Industry.


Assuntos
Comportamento do Consumidor , Paladar , Coffea/química , Coffea/metabolismo , Café/química , Café/metabolismo , Humanos , Projetos de Pesquisa , Sementes/química , Sementes/metabolismo
9.
J Sci Food Agric ; 101(15): 6586-6594, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33969494

RESUMO

BACKGROUND: Under certain climate conditions, citrus fruit may reach optimum internal maturity while the rind is still green. It is therefore customary to enhance skin colour changes by submitting early citrus varieties to ethylene degreening treatment, which implies a cost and may result in external disorders. In this study, the effect on consumer expectations (sensory and hedonic) of providing them with information about the internal maturity of non-degreened mandarins (greenish-coloured rind) was evaluated. RESULTS: Mandarins with three different external colour indices (CI), CI = -6, CI = +1 and CI = +5, and three ways of conveying information, were investigated: (i) No information (NoInfo), (ii) The text 'Mandarins ready to eat' (Text) and (iii) Text + Visualisation of mandarin flesh (orange-coloured in all cases) (Text+Flesh). Consumer expected liking, purchase intention and sensory expectations were markedly affected after receiving the different information types. The greatest effect was detected when Text+Flesh information was provided. Rind mandarin colour was also a determinant factor of how consumer expectations were modified by the received information. Moreover, our results revealed some differences among Spanish and Mexican consumers, since maturity information had a stronger effect on hedonic expectations of Mexican consumers. CONCLUSIONS: When mandarins reach CI = +5 (pale orange-coloured rind with small areas still green), letting consumers know that mandarins are ready to eat by means of Text+Flesh information may be a non-cost strategy to avoid degreening treatment or to shorten its length. © 2021 Society of Chemical Industry.


Assuntos
Citrus/metabolismo , Comportamento do Consumidor , Citrus/química , Citrus/economia , Cor , Comparação Transcultural , Frutas/química , Frutas/economia , Frutas/metabolismo , Humanos , México , Motivação , Espanha , Paladar
10.
J Food Sci Technol ; 58(2): 586-594, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33568852

RESUMO

Due to their practicality and convenience, consumption of snack foods has grown among the Brazilian population. At the same time, the demand for healthier products continues to grow, as today's consumers are increasingly concerned about their health and well-being. To meet this demand, traditional products have been reformulated to achieve a healthier nutritional profile. In this context, the aim of this study was to understand the sensory and hedonic perception of Brazilian consumers toward salty snacks. Sixty-one consumers answered a Check-all-that-apply (CATA) questionnaire, followed by an acceptance test and an attitudinal questionnaire. Four traditional salty snacks and one fit snack were sampled. The results showed that both the fit snack and the traditional snacks were well accepted by consumers. According to CATA questionnaire, the samples presented a completely different sensory profile, describing their ideal snack as: crispy, homogeneous, little cheese aroma, corn flavor, sweet residual taste and little salty taste. In addition, the attitudinal questionnaire indicated that consumers want products that bring health benefits, while maintaining their pleasant flavor and affordable price. These findings highlight that understanding consumers' desires can help new products succeed in the marketplace and also aid in marketing strategies to be used.

11.
J Food Sci Technol ; 58(11): 4167-4177, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34538901

RESUMO

Botanical origin is one of the principal factors influencing the composition and quality of honey. This study aimed to evaluate different single-flower (assa-peixe, coffee, eucalyptus, laranjeira, and vassourinha), polyfloral (silvestre), extrafloral (sugarcane), and honeydew (bracatinga) honeys with regard to their chemical, physicochemical, and physical properties; rheological behavior; bioactive compounds; and antioxidant activity. In addition, we assessed their sensory characteristics using the acceptance test and the check-all-that-apply test (CATA). All honeys were compliant with current legislation and presented Newtonian behavior. The honeys of assa-peixe, laranjeira, and coffee presented the highest viscosity, sugarcane honey showed the highest antioxidant activity, and the bracatinga honey had the highest phenolic compound content. With respect to sensory characteristics, floral honeys presented higher acceptability than did honeydew and extrafloral honeys, because honey from honeydew was negatively influenced by its bitter, alcoholic, and astringent taste and extrafloral honey by its burnt smell. These findings indicate that the botanical origin directly influences the characteristics of honeys and can be considered a factor for their differentiation.

12.
Prep Biochem Biotechnol ; 50(5): 486-493, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31900038

RESUMO

Catechol 1,2-dioxygenases catalyze catechol ring-opening, a critical step in the degradation of aromatic compounds. Cupriavidus campinensis BJ71, an efficient 2,4-dichlorophenoxyacetic acid (2,4-D)-degrading bacterial strain, was previously isolated from an environment contaminated with 2,4-D. In this study, catA encoding a catechol 1,2-dioxygenase was cloned from the BJ71 strain. The gene was 939 bp long and encoded a polypeptide of 312 amino acids with a molecular weight of 34 kDa. To investigate its enzymatic characteristics, CatA was heterologously expressed in Escherichia coli. Optimal reaction conditions for the pure enzyme were 35 °C and pH 8.0. The enzyme remained stable within a range of 25 °C-45 °C and pH 6.0-9.0, thus indicating that CatA has wide temperature and pH adaptability. After incubation at 45 °C, the enzyme activity of CatA decreased to 37.12%, but its activity was not affected by incubation at pH 9.0. The pure enzyme was able to use catechol, 4-methyl-catechol and 4-chlorocatechol as substrates. Enzyme kinetic parameters Km and Vmax were 39.97 µM and 10.68 U/mg, respectively. This is the first report of the cloning of a gene encoding a catechol 1,2-dioxygenase from a 2,4-D-degrading bacterial strain.


Assuntos
Proteínas de Bactérias/química , Catecol 1,2-Dioxigenase/química , Cupriavidus/enzimologia , Sequência de Aminoácidos , Proteínas de Bactérias/genética , Proteínas de Bactérias/isolamento & purificação , Catecol 1,2-Dioxigenase/genética , Catecol 1,2-Dioxigenase/isolamento & purificação , Clonagem Molecular , Ensaios Enzimáticos , Escherichia coli/genética , Concentração de Íons de Hidrogênio , Filogenia , Alinhamento de Sequência , Temperatura
13.
J Sci Food Agric ; 100(9): 3748-3754, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32253753

RESUMO

BACKGROUND: Honey from Tiúba stingless bees (Melipona fasciculata) produced in three different ecosystems in Brazil (Cerrado, Litoral and Baixada), as well as a sample of honey from Apis mellifera bees from the Baixada region for comparison, were evaluated regarding viscosity, color, sensory profile and sensory acceptance. RESULTS: The honey from Tiúba was less viscous, with a darker color and lower purity of the color (chroma) in relation to honey from Apis mellifera. The sensory profile firmly differentiates the honeys. The Apis honey was mainly described as opaque, viscous and adhesive. Tiúba honey from the Cerrado region stood out as semi-bright, acid taste, alcoholic, propolis, bitter taste and spicy. Tiúba honey from the Litoral region was characterized by its yellow color, woody odor and medicine flavor. The Tiúba honey from the Baixada region was described as gold colored, fluid and citric. The Tiúba honey from the Cerrado region had similar sensory acceptance to the Apis honey, probably because it was specifically characterized through attributes of odor and flavor. On the contrary, the Tiúba honey from the Litoral and from the Baixada regions was less well accepted. CONCLUSION: The honeys are different regarding their physical and sensory properties, differences that result from the bee species and from the geographical origin of the honey. This study could contribute to the creation of a standard identity and specific quality for Melipona fasciculata honey, also contributing to its production and commercialization not only in Brazil, but also internationally. © 2020 Society of Chemical Industry.


Assuntos
Abelhas/metabolismo , Mel/análise , Animais , Abelhas/classificação , Brasil , Cor , Ecossistema , Humanos , Odorantes/análise , Paladar
14.
Asian-Australas J Anim Sci ; 33(2): 339-348, 2020 02 01.
Artigo em Inglês | MEDLINE | ID: mdl-31208172

RESUMO

OBJECTIVE: This study investigates the technological and sensory profile of boneless dry-cured ham added with different contents of lactulose as a prebiotic ingredient. METHODS: In addition to the control samples (without the addition of lactulose), three treatments were formulated to contain 2, 4 or 6% lactulose. Technological (lactulose content, CIE color and texture profile analysis) and sensory (acceptance and check-all-that-applies tests) analyses were performed on the final product. RESULTS: The lactulose content in the finished product (1.86±0.23%, 3.16±0.18% and 2.51±1.35%) was lower than the lactulose originally added (2, 4 and 6% respectively). The addition of 4% and 6% lactulose made (P < 0.05) the products darker (lower L*) and redder (lower h) with higher hardness and chewiness values, when compared to control samples. The additions of 2% and 4% lactulose reduce the appearance acceptability of the products, but overall the treatments were well accepted. CONCLUSION: The use of up to 4% lactulose as a prebiotic in the production of boneless dry-cured hams provides an alternative to improving its nutritional value with little alteration in the technological characteristics and still meeting the sensory characteristics desired by consumers.

15.
J Food Sci Technol ; 57(2): 617-627, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32116371

RESUMO

This study aimed to evaluate the sensory and hedonic perceptions of chicken burger manufactured with pink pepper residue extract (PPE) added to the meat and to the chitosan film. Five samples were manufactured: CT: control, without antioxidant; DP and FP: addition of PPE to the meat batter and to the film, respectively; C1 and C2: commercial brands of chicken burgers. Consumers characterized the samples using the overall liking test and Check-all-that-apply questions. The samples showed a medium-high level of acceptance and no significant differences were found between them. DP was the farthest sample from the ideal and FP showed positive results, since its characteristics were like the commercial samples. The direct extract application may lead to a reduction in the liking of chicken burgers, demonstrating that the technology of active films is a viable alternative to the use of natural antioxidant extracts in meat products.

16.
Appetite ; 142: 104346, 2019 11 01.
Artigo em Inglês | MEDLINE | ID: mdl-31278955

RESUMO

The successful promotion of vegetable consumption by children requires a deep understanding of children's vegetable preferences as well as the factors shaping them throughout childhood. This study analyzed children vegetable liking in four different age ranges (5-6, 7-8, 9-10 and 11-12 years old) in Chile, China and the United States. Three hundred and eighty-four children completed this study. All participants tasted and rated 14 different vegetables for liking and described the samples using Check-All-That-Apply (CATA). We found significant differences in degree of overall liking among children from the three countries (p < 0.001). Specifically, children in China gave higher overall liking scores than children in the US, and in the US higher than in Chile. Child age and gender did not influence children's vegetable overall liking across the three countries. Across all countries and age groups, liking of taste and texture were the best predictors of children overall liking. The penalty analysis of CATA selections by children showed that the mean impact of the attributes that children used to describe the samples on their liking varied among countries, with the descriptors having the least impact on liking for Chinese children.


Assuntos
Comparação Transcultural , Preferências Alimentares/etnologia , Verduras , Criança , Pré-Escolar , Chile , China , Escolaridade , Feminino , Humanos , Masculino , Sensação , Olfato , Paladar , Estados Unidos
17.
J Food Sci Technol ; 56(6): 3126-3135, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31205367

RESUMO

The study aimed to investigate the effect of introducing texturized soy protein (TSP) at different levels (15% and 30%) with and without nutritional yeast as flavour enhancer on the sensory and instrumental quality of beef meatballs, compared to a soy and yeast-free control. Proximate analysis, colour, instrumental texture, cook loss, and sensory quality were investigated. Sixty participants assessed the samples using Check-all-that-apply (CATA) questions and hedonic scales. Overall, the texture of all TSP-containing samples received significantly higher acceptability scores than control, while 15% TSP with yeast received the highest flavour and overall acceptability scores. Penalty-lift analysis of CATA terms identified the main drivers for liking as "moist looking", "juicy", "soft" and "crumbly and easy to cut". Control samples were significantly more often associated than the other recipes to the term "hard", a key driver for dislike and the least associated to "soft" and "crumbly and easy to cut". Adding 15-30% TSP with or without yeast inclusion could be beneficial for the development of future meat hybrids with acceptable sensory quality.

18.
Antonie Van Leeuwenhoek ; 111(12): 2293-2301, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29959655

RESUMO

The objective was to understand the roles of multiple catechol dioxygenases in the type strain Sphingobium scionense WP01T (Liang and Lloyd-Jones in Int J Syst Evol Microbiol 60:413-416, 2010a) that was isolated from severely contaminated sawmill soil. The dioxygenases were identified by sequencing, examined by determining the substrate specificities of the recombinant enzymes, and by quantifying gene expression following exposure to model priority pollutants. Catechol dioxygenase genes encoding an extradiol xylE and two intradiol dioxygenases catA and clcA that are highly similar to sequences described in other sphingomonads are described in S. scionense WP01T. The distinct substrate specificities determined for the recombinant enzymes confirm the annotated gene functions and suggest different catabolic roles for each enzyme. The role of the three enzymes was evaluated by analysis of enzyme activity in crude cell extracts from cells grown on meta-toluate, benzoate, biphenyl, naphthalene and phenanthrene which revealed the co-induction of each enzyme by different substrates. This was corroborated by quantifying gene expression when cells were induced by biphenyl, naphthalene and pentachlorophenol. It is concluded that the ClcA and XylE enzymes are recruited in pathways that are involved in the degradation of chlorinated aromatic compounds such as pentachlorophenol, the XylE and ClcA enzymes will also play a role in degradation pathways that produce alkylcatechols, while the three enzymes ClcA, XylE and CatA will be simultaneously involved in pathways that generate catechol as a degradation pathway intermediate.


Assuntos
Proteínas de Bactérias/metabolismo , Catecol 1,2-Dioxigenase/metabolismo , Catecol 2,3-Dioxigenase/metabolismo , Dioxigenases/metabolismo , Sphingomonadaceae/enzimologia , Proteínas de Bactérias/genética , Benzoatos/metabolismo , Compostos de Bifenilo/metabolismo , Catecol 1,2-Dioxigenase/genética , Catecol 2,3-Dioxigenase/genética , Catecóis/metabolismo , Clonagem Molecular , Dioxigenases/genética , Ensaios Enzimáticos , Escherichia coli/genética , Escherichia coli/metabolismo , Expressão Gênica , Vetores Genéticos/química , Vetores Genéticos/metabolismo , Cinética , Naftalenos/metabolismo , Pentaclorofenol/metabolismo , Fenantrenos/metabolismo , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Microbiologia do Solo , Sphingomonadaceae/genética , Sphingomonadaceae/isolamento & purificação , Especificidade por Substrato , Tolueno/metabolismo , Xilose/metabolismo
19.
J Sci Food Agric ; 98(3): 905-913, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-28692754

RESUMO

BACKGROUND: The challenges of malnutrition and urbanization in Africa demand the development of acceptable, affordable, nutritious complementary-type foods. Biscuits (i.e. cookies; a popular snack) from whole grain staples are an option. The present study aimed to relate check-all-that-applies (CATA) sensory profiles of sorghum-cowpea composite biscuits compared to economic commercial refined wheat biscuits with hedonic ratings by low income consumers. In addition, the nutritional composition and protein quality, L* a* b* colour and texture of the biscuits were determined. RESULTS: The CATA method is suitable for rapidly determining which attributes consumers perceive in food products and relating these to acceptability. Consumers preferred the lighter, more yellow wheat biscuits with ginger, vanilla, sweet and cinnamon flavours compared to the stronger flavours (sorghum, beany and nutty) and harder but brittle, grittier, dry and rough textured sorghum or sorghum-cowpea biscuits. However, a substantial proportion of consumers also liked the latter biscuits. The composite biscuits had higher dietary fibre content and a similar protein quality to the standards. CONCLUSIONS: Whole grain sorghum-cowpea biscuits could serve as acceptable value-added nutritious complementary snacks for consumers in sub-Saharan Africa. The biscuits are simple to produce for the creation of viable small enterprises. © 2017 Society of Chemical Industry.


Assuntos
Pão/análise , Sorghum/química , Vigna/química , África , Preferências Alimentares , Humanos , Valor Nutritivo , Pobreza , Sorghum/metabolismo , Paladar , Vigna/metabolismo
20.
J Sci Food Agric ; 98(3): 1179-1187, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-28737277

RESUMO

BACKGROUND: The aquaculture sector needs to increase the diversity fish species and their processed products to cover rising consumer demands. Candidates for this diversification have been identified to be meagre, greater amberjack, pikeperch and wreckfish. Yet scientific knowledge on their sensory profiles and consumer hedonic responses is scarce. The aim of the current study was to investigate these aspects, since they are essential for product development and market targeting. RESULTS: Species exhibited different sensory profiles with the exception of the odor/flavor profiles of meagre and greater amberjack, which were similar. Texture was more important than odor/flavor in explaining interspecies differences. Yet the hedonic responses were equally related to texture and odor/flavor. None of the species received negative hedonic scores. Both positive and negative hedonic drivers were identified within the odor/flavor and texture modalities. CONCLUSION: The distinct profiles of meagre, greater amberjack, pikeperch and wreckfish make these fish species valuable first materials for new product development and for covering markets with different sensory preferences. Differences in fish texture are more easily perceivable, yet small variations in fish odor/flavor can have a great impact on consumers' hedonic responses. © 2017 Society of Chemical Industry.


Assuntos
Produtos Pesqueiros/análise , Peixes/classificação , Adolescente , Adulto , Animais , Aquicultura , Feminino , Produtos Pesqueiros/classificação , Peixes/crescimento & desenvolvimento , Aromatizantes/análise , Preferências Alimentares , Humanos , Masculino , Pessoa de Meia-Idade , Odorantes/análise , Paladar , Adulto Jovem
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