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1.
Sensors (Basel) ; 24(5)2024 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-38475148

RESUMEN

Ensuring precise angle measurement during surgical correction of orientation-related deformities is crucial for optimal postoperative outcomes, yet there is a lack of an ideal commercial solution. Current measurement sensors and instrumentation have limitations that make their use context-specific, demanding a methodical evaluation of the field. A systematic review was carried out in March 2023. Studies reporting technologies and validation methods for intraoperative angular measurement of anatomical structures were analyzed. A total of 32 studies were included, 17 focused on image-based technologies (6 fluoroscopy, 4 camera-based tracking, and 7 CT-based), while 15 explored non-image-based technologies (6 manual instruments and 9 inertial sensor-based instruments). Image-based technologies offer better accuracy and 3D capabilities but pose challenges like additional equipment, increased radiation exposure, time, and cost. Non-image-based technologies are cost-effective but may be influenced by the surgeon's perception and require careful calibration. Nevertheless, the choice of the proper technology should take into consideration the influence of the expected error in the surgery, surgery type, and radiation dose limit. This comprehensive review serves as a valuable guide for surgeons seeking precise angle measurements intraoperatively. It not only explores the performance and application of existing technologies but also aids in the future development of innovative solutions.


Asunto(s)
Cirugía Asistida por Computador , Cirugía Asistida por Computador/métodos , Fluoroscopía/métodos
2.
An Acad Bras Cienc ; 94(suppl 1): e20210805, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-35508022

RESUMEN

Carbonaceous aerosols can affect climate, especially particles containing black carbon (BC). BC originated from the incomplete combustion of fossil fuel and biomass, which can heat the atmosphere and increase ice melting, but little is known about BC sources to Antarctica. We quantified the contribution of distant origin (biomass burning) and local emissions (fossil fuel) to atmospheric BC concentration in the King George Island (Antarctic Peninsula) and the Southern Ocean. We examine the BC concentrations using a multi-wavelength Aethalometer AE-33 and AE-42 aboard the Brazilian Oceanographic Research Ship Almirante Maximiano. The results indicate that the region is influenced by local sources and air masses coming from surrounding continents. Fossil fuel combustion was the major source of carbonaceous aerosols in the region, whereas the total average concentration was 41.8 ± 22.8 ng m-3. The findings indicate a contribution of biomass burning coming from low and mid-latitudes of South America over the Antarctic Peninsula and the Southern Ocean around 62ºS latitude. We demonstrated that fossil fuel is the main contributor to atmospheric BC concentration for the Austral summer and autumn. Scientific stations, local tourism, and traffic are possible local BC sources. Our work invokes the urgency of questionable sustainability issues about Antarctica exploration.


Asunto(s)
Contaminantes Atmosféricos , Combustibles Fósiles , Aerosoles/análisis , Contaminantes Atmosféricos/análisis , Regiones Antárticas , Océano Atlántico , Biomasa , Brasil , Carbono/análisis , Monitoreo del Ambiente/métodos , Combustibles Fósiles/análisis , Estaciones del Año
3.
Odontology ; 110(1): 62-69, 2022 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-34213683

RESUMEN

The aim of this study was to evaluate the effect of SPP with either fetal bovine serum (FBS) or deionized water (DW) on the bond strength (µTBS) of a Universal adhesive to dentin, in both etch-and-rinse (ER) and self-etch (SE) modes. The kinematic viscosity (cSt) of FBS and DW was measured at 25 °C ± 0.1 ºC. Seventy-two sound human molars were sectioned and randomly divided into three groups according to the SPP conditions: (1) Control (0 cm H2O), (2) SPP (15 cm H2O) with FBS, (3) SPP (15 cm H2O) with DW. Each group was subdivided (n = 10) based on the bonding modes: ER (37% phosphoric acid + ScothBond Universal Adhesive) or SE (ScothBond Universal Adhesive). Samples were then submitted to µTBS. Data were analyzed by Student's t test, two-way ANOVA and Tukey tests (p < 0.05). The cSt results showed that DW (23.59 ± 0.39) had significantly higher values than FBS (22.33 ± 0.06). With regard to SPP, the control group (36.1 MPa) had significantly higher values of µTBS when compared to the SPP using FBS (31.06 MPa) and SPP with DW (26.55 MPa). According to ANOVA, the bonding modes and the interaction of simulated pulpal pressure (SPP) did not statistically influence the results (p < 0.05). The presence of SPP reduced the bond strength of Universal adhesive to dentin. DW during SPP had significantly reduced bonding values when compared to FBS. Bonding strategies were not affected by SPP when evaluated in a short period of time (24 h).


Asunto(s)
Recubrimiento Dental Adhesivo , Recubrimientos Dentinarios , Resinas Compuestas , Cementos Dentales , Dentina , Humanos , Ensayo de Materiales , Cementos de Resina , Resistencia a la Tracción
4.
Nucleic Acids Res ; 47(5): 2681-2698, 2019 03 18.
Artículo en Inglés | MEDLINE | ID: mdl-30726994

RESUMEN

Most eukaryotic expression systems make use of host-cell nuclear transcriptional and post-transcriptional machineries. Here, we present the first generation of the chimeric cytoplasmic capping-prone phage polymerase (C3P3-G1) expression system developed by biological engineering, which generates capped and polyadenylated transcripts in host-cell cytoplasm by means of two components. First, an artificial single-unit chimeric enzyme made by fusing an mRNA capping enzyme and a DNA-dependent RNA polymerase. Second, specific DNA templates designed to operate with the C3P3-G1 enzyme, which encode for the transcripts and their artificial polyadenylation. This system, which can potentially be adapted to any in cellulo or in vivo eukaryotic expression applications, was optimized for transient expression in mammalian cells. C3P3-G1 shows promising results for protein production in Chinese Hamster Ovary (CHO-K1) cells. This work also provides avenues for enhancing the performances for next generation C3P3 systems.


Asunto(s)
Núcleo Celular/genética , Citoplasma/genética , ARN Polimerasas Dirigidas por ADN/genética , Transcripción Genética , Animales , Células CHO , Cricetulus , Citoplasma/química , ARN Polimerasas Dirigidas por ADN/química , Células Eucariotas/química , Células Eucariotas/metabolismo , Humanos , Poli A/genética , Poliadenilación/genética
5.
Knee Surg Sports Traumatol Arthrosc ; 29(5): 1453-1460, 2021 May.
Artículo en Inglés | MEDLINE | ID: mdl-33386879

RESUMEN

PURPOSE: This study was designed to evaluate the clinical and radiographic results of arthroscopic treatment of femoroacetabular impingement (FAI) using the technique of initial access to the peripheral compartment. It is based on a single surgeon large case series with a minimum of 2 years follow-up. METHODS: Prospective longitudinal study with consecutive patients. Inclusion criteria were the presence of FAI syndrome that had failed non-operative treatment and had a hip arthroscopy with initial access to the peripheral compartment. Exclusion criteria were previous hip surgery, patients younger than 16 or older than 60 years, Tönnis grade ≥ 2 osteoarthritis, hip dysplasia based on radiographic evidence of LCEA less than 25° and workers compensation cases. One hundred and sixty hips met the inclusion criteria, 84 were female and 70 were male patients (six bilateral cases), with a median age of 36 years (range 16-59). RESULTS: The median alpha angle correction was 22.6º (range 5.9-46.7) (p < 0.01) and the average LCEA correction when acetabuloplasty was undertaken was 6.5º (range - 1.4-20.8) (p < 0.01). The mean NAHS at baseline was 56.1 (range 16-96) and improved to 83.2 at the last follow up (range 44-100) for the patients that had no additional procedure (p < 0.01). The mean average improvement was 27.7º points (range - 16-73). No iatrogenic labral perforation and no full-thickness chondral damage were recorded during the arthroscopic procedures. CONCLUSIONS: Favourable outcomes are reported for the arthroscopic treatment of FAI with initial access to the peripheral compartment. The technique is protective against iatrogenic chondral and labral damage, more conservative to the joint capsule, but the mean traction time was relatively long when suture anchors were used. The results are comparable to the classic initial central compartment approach. LEVEL OF EVIDENCE: Level IV.


Asunto(s)
Artroscopía/métodos , Pinzamiento Femoroacetabular/cirugía , Articulación de la Cadera/cirugía , Acetabuloplastia/métodos , Adolescente , Adulto , Femenino , Estudios de Seguimiento , Cadera/cirugía , Luxación de la Cadera/epidemiología , Humanos , Estudios Longitudinales , Masculino , Persona de Mediana Edad , Osteoartritis/epidemiología , Estudios Prospectivos , Resultado del Tratamiento , Adulto Joven
6.
Clin Oral Investig ; 24(3): 1321-1331, 2020 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-31297659

RESUMEN

OBJECTIVES: The purpose of this study was to evaluate the 2-year success of resin composite restorations in non-carious cervical lesions (NCCLs) using the direct or semi-direct techniques. MATERIALS AND METHODS: Thirty volunteers presenting with at least two NCCLs were included. Each participant received one restoration using the direct technique and the other using the semi-direct technique, totaling 60 restorations. Time for completing the treatment was computed. Assessments at baseline, 7 days, and 6, 12, and 24 months were performed using the modified United States Public Health Service criteria. Descriptive analysis was reported as a percentage of successful treatments. For inferential analysis, the Student t test was used to evaluate the differences between extension, depth, and time. The chi-square/Fisher tests were used to compare treatment success after each period (α = 0.05). The results were evaluated by using the Kaplan-Meier survival analysis. RESULTS: Differences were detected regarding mean ± standard deviation time, in which direct and semi-direct procedures were accomplished in 21.8 (± 14.5) and 35.3 (± 19.9) min, respectively. Of the 60 restorations placed, 7 failed in the direct group while 8 failed in the semi-direct group up to 2 years. No differences were detected between restorative protocols. The cumulative survival was 88.5% and 88.4% for the direct technique and semi-direct techniques after 24 months, respectively. CONCLUSION: The tested restorative protocols present similar results for NCCLs within the studied periods. CLINICAL RELEVANCE: The semi-direct technique exhibited clinical performance similar to direct technique for NCCL, demonstrating an alternative for restorations of these lesions.


Asunto(s)
Resinas Compuestas , Restauración Dental Permanente/métodos , Cuello del Diente/patología , Anciano , Adaptación Marginal Dental , Femenino , Humanos , Masculino , Persona de Mediana Edad
7.
Am J Dent ; 32(6): 299-305, 2019 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-31920056

RESUMEN

PURPOSE: To evaluate the effect of a zinc chloride (ZnCl2) solution (2% wt), incorporated into a two-step etch-and-rinse adhesive system (AS), on microtensile bond strength (µTBS) to caries-affected dentin (CAD). METHODS: First, the degree of conversion (DC) of the AS with and without the ZnCl2 solution was evaluated by Fourier transform infrared spectroscopy (FTIR). Second, beam-shaped samples (7 mm × 2 mm × 1 mm) were prepared with the AS containing the ZnCl2 solution to perform flexural strength (FS) tests. For µTBS testing purposes, CAD surfaces were etched with phosphoric acid and randomly divided into three groups (n=10) : ZnCl2- AS with ZnCl2; NT (no treatment)- AS without ZnCl2; and CHX - pretreatment with 2% chlorhexidine solution. AS (Adper Single Bond 2) was applied according to the manufacturer's instructions, and resin composite restorations were built up. After 24 hours, the resin-dentin blocks were sectioned into specimens (0.8 mm2), and then subjected to micro;TBS testing immediately following, or after 6 or 12 months of water storage (WS). The adhesive interface was evaluated by scanning electron microscope. Both µTBS and FS tests were performed using a universal testing machine ( 0.5 mm/minute). FS and DC data were submitted to the Student t-test, and µTBS data were subjected to two-way ANOVA and Tukey's test (α= 0.05). RESULTS: DC was not affected by ZnCl2 incorporation into AS (P= 0.2527). Higher FS values were obtained in the group with ZnCl2 added to AS. Regarding µTBS, the mean of the NT group was statistically higher than that of the ZnCl2 or the CHX groups (P< 0.001(, regardless of WS, but the latter groups did not differ from each other. µTBS at 24 hours was statistically superior to that of 6 and 12 months of WS (P< 0.001).


Asunto(s)
Recubrimiento Dental Adhesivo , Recubrimientos Dentinarios , Grabado Ácido Dental , Bisfenol A Glicidil Metacrilato , Cloruros , Resinas Compuestas , Análisis del Estrés Dental , Dentina , Resistencia Flexional , Humanos , Ensayo de Materiales , Cementos de Resina , Resistencia a la Tracción , Compuestos de Zinc
8.
Am J Orthod Dentofacial Orthop ; 155(2): 243-253, 2019 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-30712696

RESUMEN

INTRODUCTION: This study evaluated the efficacy of photodynamic inactivation (PDI) with hematoporphyrin IX (H) and modified hematoporphyrin IX (MH) at 10 µmol/L, using a blue light-emitting diode (LED), fluence of 75 J/cm,2 over planktonic cultures and biofilm of Streptococcus mutans (UA 159). METHODS: Suspensions containing 107 cells/mL were tested under different experimental conditions: a) H and LED (H+L+), b) MH and LED (MH+L+), c) only LED (P-L+), d) only H (H+L-), e) only MH (MH+L-), and f) control group, no LED or photosensitizer treatment (P-L-). The study also evaluated the effect of PDI on S mutans biofilm on metallic or ceramic brackets bonded on specimens of human teeth. The strains were seeded onto Mitis salivarius-bacitracin-sacarose agar to determine the number of colony-forming units. RESULTS: H and MH under LED irradiation were effective on planktonic cultures (P <0.0001). H and MH (H+L+ and MH+L+) caused a reduction of 3.80 and 6.78 log10 CFU/mL. PDI with the use of H or MH and LED exerted a strong antimicrobial effect over S mutans showing 54% and 100% reduction, respectively. PDI on S mutans biofilm on metallic and ceramic brackets with the use of H was not effective (P = 0.0162, P = 0.1669), however, MH caused a significant reduction of 44% and 53% of the cell count on metallic and ceramic brackets, respectively (P = 0.0020, P = 0.004). CONCLUSIONS: In vitro planktonic cultures with the use of H or MH and LED exerted significant antimicrobial activity. No effect was observed on S mutans biofilm on either bracket type with the use of H, MH showed better results, suggesting a promising use against dental caries and white spot lesions.


Asunto(s)
Biopelículas/efectos de los fármacos , Biopelículas/efectos de la radiación , Caries Dental/etiología , Caries Dental/prevención & control , Hematoporfirinas/farmacología , Ortodoncia Correctiva/efectos adversos , Fotoquimioterapia , Fármacos Fotosensibilizantes/farmacología , Plancton/efectos de los fármacos , Plancton/efectos de la radiación , Streptococcus mutans/efectos de los fármacos , Streptococcus mutans/efectos de la radiación , Humanos , Técnicas In Vitro , Streptococcus mutans/fisiología
9.
J Biol Chem ; 291(14): 7583-93, 2016 Apr 01.
Artículo en Inglés | MEDLINE | ID: mdl-26887944

RESUMEN

Human mitoNEET (mNT) is the first identified Fe-S protein of the mammalian outer mitochondrial membrane. Recently, mNT has been implicated in cytosolic Fe-S repair of a key regulator of cellular iron homeostasis. Here, we aimed to decipher the mechanism by which mNT triggers its Fe-S repair capacity. By using tightly controlled reactions combined with complementary spectroscopic approaches, we have determined the differential roles played by both the redox state of the mNT cluster and dioxygen in cluster transfer and protein stability. We unambiguously demonstrated that only the oxidized state of the mNT cluster triggers cluster transfer to a generic acceptor protein and that dioxygen is neither required for the cluster transfer reaction nor does it affect the transfer rate. In the absence of apo-acceptors, a large fraction of the oxidized holo-mNT form is converted back to reduced holo-mNT under low oxygen tension. Reduced holo-mNT, which holds a [2Fe-2S](+)with a global protein fold similar to that of the oxidized form is, by contrast, resistant in losing its cluster or in transferring it. Our findings thus demonstrate that mNT uses an iron-based redox switch mechanism to regulate the transfer of its cluster. The oxidized state is the "active state," which reacts promptly to initiate Fe-S transfer independently of dioxygen, whereas the reduced state is a "dormant form." Finally, we propose that the redox-sensing function of mNT is a key component of the cellular adaptive response to help stress-sensitive Fe-S proteins recover from oxidative injury.


Asunto(s)
Proteínas Hierro-Azufre/metabolismo , Proteínas Mitocondriales/metabolismo , Estrés Oxidativo/fisiología , Humanos , Proteínas Hierro-Azufre/química , Proteínas Hierro-Azufre/genética , Proteínas Mitocondriales/química , Proteínas Mitocondriales/genética , Oxidación-Reducción
10.
Sensors (Basel) ; 17(12)2017 Dec 01.
Artículo en Inglés | MEDLINE | ID: mdl-29194414

RESUMEN

The market for interactive platforms is rapidly growing, and touchscreens have been incorporated in an increasing number of devices. Thus, the area of smart objects and devices is strongly increasing by adding interactive touch and multimedia content, leading to new uses and capabilities. In this work, a flexible screen printed sensor matrix is fabricated based on silver ink in a polyethylene terephthalate (PET) substrate. Diamond shaped capacitive electrodes coupled with conventional capacitive reading electronics enables fabrication of a highly functional capacitive touchpad, and also allows for the identification of marked objects. For the latter, the capacitive signatures are identified by intersecting points and distances between them. Thus, this work demonstrates the applicability of a low cost method using royalty-free geometries and technologies for the development of flexible multitouch touchpads for the implementation of interactive and object recognition applications.

11.
J Contemp Dent Pract ; 18(5): 352-357, 2017 May 01.
Artículo en Inglés | MEDLINE | ID: mdl-28512271

RESUMEN

INTRODUCTION: This study evaluated the fluorescence intensity (FI) of different brands of composite resins (CRs) and compare those values with the FI of human tooth, under the action of cigarette smoke (CI), coffee (CA), and soft drink (CO), measured by direct spectrometry. MATERIALS AND METHODS: A total of 30 specimens of each brand (Filtek Z350, Esthet-X, Amelogen, Durafill) were made. Others 30 tooth specimens (3 mm/diameter) were obtained from human molars using a trephine bur. The specimens were randomly divided into three groups (n = 10), according to substances: CI, CA, CO. The FI was directly measured using an optic fiber associated with a spectrometer and was measured at baseline and after staining. Data were submitted to Kruskal-Wallis, Dunnett, and Dunn tests. RESULTS: Staining influenced FI mean values among CRs and between those with human tooth. Z showed the closest FI mean values of tooth after staining. CONCLUSION: Staining beverages and cigarette smoke negatively influenced on FI of CR and human tooth. CLINICAL SIGNIFICANCE: The study shows darkening treatments influenced on the fluorescent property of the dental tissues and restorative materials according to the direct spectrometry analysis.


Asunto(s)
Bebidas Gaseosas/efectos adversos , Café/efectos adversos , Resinas Compuestas/química , Nicotiana , Humo/efectos adversos , Decoloración de Dientes/diagnóstico por imagen , Fumar Cigarrillos/efectos adversos , Fluorescencia , Humanos , Espectrometría de Fluorescencia , Análisis Espectral , Decoloración de Dientes/etiología
12.
J Biol Chem ; 289(41): 28070-86, 2014 Oct 10.
Artículo en Inglés | MEDLINE | ID: mdl-25012650

RESUMEN

In eukaryotes, mitochondrial iron-sulfur cluster (ISC), export and cytosolic iron-sulfur cluster assembly (CIA) machineries carry out biogenesis of iron-sulfur (Fe-S) clusters, which are critical for multiple essential cellular pathways. However, little is known about their export out of mitochondria. Here we show that Fe-S assembly of mitoNEET, the first identified Fe-S protein anchored in the mitochondrial outer membrane, strictly depends on ISC machineries and not on the CIA or CIAPIN1. We identify a dedicated ISC/export pathway in which augmenter of liver regeneration, a mitochondrial Mia40-dependent protein, is specific to mitoNEET maturation. When inserted, the Fe-S cluster confers mitoNEET folding and stability in vitro and in vivo. The holo-form of mitoNEET is resistant to NO and H2O2 and is capable of repairing oxidatively damaged Fe-S of iron regulatory protein 1 (IRP1), a master regulator of cellular iron that has recently been involved in the mitochondrial iron supply. Therefore, our findings point to IRP1 as the missing link to explain the function of mitoNEET in the control of mitochondrial iron homeostasis.


Asunto(s)
Proteína 1 Reguladora de Hierro/química , Hierro/metabolismo , Mitocondrias/metabolismo , Proteínas de Transporte de Membrana Mitocondrial/química , Membranas Mitocondriales/metabolismo , Proteínas Mitocondriales/química , Animales , Escherichia coli/genética , Escherichia coli/metabolismo , Regulación de la Expresión Génica , Células HeLa , Células Hep G2 , Homeostasis , Humanos , Peróxido de Hidrógeno/química , Proteína 1 Reguladora de Hierro/genética , Proteína 1 Reguladora de Hierro/metabolismo , Ratones , Ratones Transgénicos , Mitocondrias/química , Proteínas de Transporte de Membrana Mitocondrial/genética , Proteínas de Transporte de Membrana Mitocondrial/metabolismo , Membranas Mitocondriales/química , Proteínas del Complejo de Importación de Proteínas Precursoras Mitocondriales , Proteínas Mitocondriales/genética , Proteínas Mitocondriales/metabolismo , Óxido Nítrico/química , Oxidación-Reducción , Pliegue de Proteína , Estabilidad Proteica , Estructura Terciaria de Proteína , Transporte de Proteínas , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Transducción de Señal
13.
J Toxicol Environ Health A ; 78(11): 685-96, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-26039746

RESUMEN

In recent years, efforts have been undertaken to reduce the volume of residual waste through sorting and recycling. The waste management and recycling sector is thriving and the number of workers there is increasing. In this context, prior knowledge of the risks to which workers may be exposed is of crucial importance, and preventive measures need to be put in place to accurately identify and quantify those risks. This study aimed to assess occupational risk of exposure to biological agents (viable bacteria and fungi) in a Portuguese waste packaging glass sorting plant. Air samples were collected from selected locations in waste sorting cabins (critical area, CA), administrative services (noncritical area, NCA) and outdoors (control point, CP). Duplicate air samples were collected through an impaction method. The investigation was carried out over an 8-mo period with two collection periods, autumn/winter (AW) and spring/summer (SS), in order to access the influence of any seasonal variation. In the 36 air samples collected, 319 bacterial and 196 mold identifications were performed. Air samples revealed existence of high environmental contamination by bacteria (1.6 × 10(4) colony forming units [cfu]/m(3)) and fungi (1.5 × 10(4) cfu/m(3)). The predominant bacterial genus was Staphylococcus (coagulase negative) with values ranging from 29.6 to 60% of the total count of bacteria. Genera Bacillus, Micrococcus, and Staphylococcus (coagulase negative) were also present at all sampling sites, regardless of the season. However, the counts of these genera, in the CA, were higher in warmer seasons. The genus Penicillium was the most frequent genus present with an approximate value of 95% of total fungal count in the CA. Seasonal variation was a significant factor for total bacteria and fungi, except for NCA versus CP. Overall, the highest levels of bacterial and fungal species (10(4) cfu/m(3)) were found in the waste sorting cabin (CA). These results highlight the importance of proper design and risk evaluation when planning a new waste facility, such that working conditions minimize proliferation of biological agents in the workplace.


Asunto(s)
Microbiología del Aire , Contaminantes Ocupacionales del Aire/análisis , Bacterias/aislamiento & purificación , Monitoreo del Ambiente , Hongos/aislamiento & purificación , Exposición Profesional , Eliminación de Residuos , Recuento de Colonia Microbiana , Humanos , Portugal , Medición de Riesgo , Estaciones del Año
14.
Acta Odontol Scand ; 72(1): 31-5, 2014 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-24255965

RESUMEN

BACKGROUND: Reduced surface tension of liquids results in higher surface wetting ability and diffusivity by the substrate. OBJECTIVES: The objective of this study was to evaluate the influence of the Grander Technology in reducing the surface tension of adhesive systems. METHODS: Two adhesive systems (self-etch and total-etch) were modified by physical contact with the Grander system Flexible unit to revitalize water, for 48 h. Surface tension of adhesive systems and water in normal and grander-modified conditions was measured with a goniometer. RESULTS: The results showed a reduction of surface tension for all conditions grander-modified between 3-15%. CONCLUSIONS: Grander Technology was effective in reducing the surface tension of the Single Bond and Clearfil SE Bond adhesive systems. Clinical significance. Grander technology was employed to restructure the molecular structure of water-based adhesive systems, which can increase their wetness capacity and therefore ensure a greater diffusibility.


Asunto(s)
Cementos Dentales , Odontología , Tensión Superficial , Humanos
15.
Int J Periodontics Restorative Dent ; 0(0): 1-17, 2024 05 31.
Artículo en Inglés | MEDLINE | ID: mdl-38820272

RESUMEN

The objective of this work is to report a restorative approach with composite resin applied in reverse in the aesthetic resolution of darkened anterior teeth. A 27-year-old female patient presented with the main complaint of dissatisfaction with the aesthetics of her smile due to dental darkening, a result of childhood trauma, without painful symptoms, pathological clinical signs or change in care. In the intraoral examination, a darkening of teeth 11 and 21 was observed, which radiographically showed thickening of the periodontal ligament, with disharmonious configuration of the marginal gingiva. From the clinical and radiographic characteristics, we opted for periodontal intervention for recovery and gingival levelling, followed by endodontic intervention and aesthetic restorative restoration. The restorative step was carried out sequentially by internal tooth bleaching, removal of dentin still pigmented after bleaching and enamel preservation. Soon after, it was restored in an alternative way and layered using a technique called reverse, using strips of polyethylene fiber over the dentin resin. The technique preserved the remaining enamel with the maintenance of the original remaining guides, proving to be a viable conservative alternative since of the diagnosis to the restorative conclusion in view of the longitudinal confirmation of the restorative efficacy after 1 year.

16.
J Appl Oral Sci ; 32: e20230359, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-38537030

RESUMEN

OBJECTIVE: This study aimed to analyze the longitudinal bond strength of a universal adhesive and chemically characterize the dentin substrate under different acid etching protocols. METHODOLOGY: Dentin samples were etched with polyacrylic acid 25% (PAA) for 10 seconds (n=3) and phosphoric acid 32% (PA) for 15 seconds (n=3) and analyzed by Fourier transform infrared spectroscopy - attenuated total reflectance (FTIR-ATR) before and after treatment. For collagen degradation, samples (n=12) were divided into 3 groups: PAA, PA, and Deionized water (control), and analyzed by the quantity of solubilized type I collagen C-terminal cross-linked telopeptides and solubilized C-terminal peptide in relation to total protein concentration (ICTPtp and CTXtp) and by their ultimate tensile strength (UTS). For the adhesive interface analysis, dentin samples (n=72) were divided into 3 groups: PAA, PA, and Self-etch (SE), and subdivided into 2 groups: 24 h (baseline) and 1 year. The following tests were performed: microtensile bond strength (µTBS) (n=48), scanning electron microscopy (SEM) (n=12), and nanoleakage (n=12). RESULTS: The FTIR of PAA showed lower reduction of the peaks in the phosphate group when compared to PA. For ICTPtp, PA showed a significantly higher value. For CTXtp, PA and PAA groups failed to statically differ from each other. UTS was significantly lower for PA. For µTBS, storage time significantly affected bond strength. The results were unaffected by the etching protocol. For SEM, after 1 year, PA had little evidence of degradation in the upper third of the adhesive interface in comparison to the other groups. Nanoleakage showed no considerable silver impregnation after 1 year in the SE group. CONCLUSION: The use of PAA prior to a universal adhesive (when compared to PA) represents a less aggressive type of etching to dentin. However, self-etching still seems to be the best option for universal adhesive systems that have functional monomers in their composition.


Asunto(s)
Recubrimiento Dental Adhesivo , Cementos Dentales , Dentina , Ácidos Fosfóricos , Resistencia a la Tracción , Microscopía Electrónica de Rastreo , Recubrimientos Dentinarios/química , Ensayo de Materiales , Cementos de Resina/química
17.
Prosthet Orthot Int ; 2024 Jan 04.
Artículo en Inglés | MEDLINE | ID: mdl-38175034

RESUMEN

This systematic review aims to assess and summarize the current landscape in exoskeletons and orthotic solutions developed for upper limb medical assistance, which are partly or fully produced using 3-dimensional printing technologies and contain at least the elbow or the shoulder joints. The initial search was conducted on Web of Science, PubMed, and IEEEXplore, resulting in 92 papers, which were reduced to 72 after removal of duplicates. From the application of the inclusion and exclusion criteria and selection questionnaire, 33 papers were included in the review, being divided according to the analyzed joints. The analysis of the selected papers allowed for the identification of different solutions that vary in terms of their target application, actuation type, 3-dimensional printing techniques, and material selection, among others. The results show that there has been far more research on the elbow joint than on the shoulder joint, which can be explained by the relative complexity of the latter. Moreover, the findings of this study also indicate that there is still a gap between the research conducted on these devices and their practical use in real-world conditions. Based on current trends, it is anticipated that the future of 3-dimensional printed exoskeletons will revolve around the use of flexible and high-performance materials, coupled with actuated devices. These advances have the potential to replace the conventional fabrication methods of exoskeletons with technologies based on additive manufacturing.

18.
Head Neck ; 2024 May 21.
Artículo en Inglés | MEDLINE | ID: mdl-38770972

RESUMEN

BACKGROUND: The current study presents the effort of a global collaborative group to review the management and outcomes of malignant tumors of the skull base worldwide. PATIENTS AND METHODS: A total of 28 institutions contributed data on 3061 patients. Analysis evaluated clinical variables, survival outcomes, and multivariable factors associated with outcomes. RESULTS: The median age was 56 years (IQR 44-67). The open surgical approach was used in 55% (n = 1680) of cases, endoscopic resection was performed in 36% (n = 1087), and the combined approach in 9.6% (n = 294). With a median follow-up of 7.1 years, the 5-year OS DSS and RFS were 65%, 71.7% and 53%, respectively. On multivariable analysis, older age, comorbidities, histology, dural/intracranial involvement, positive margins, advanced stage, and primary site were independent prognostic factors for OS, DSS, and RFS. Adjuvant RT was a protective prognostic factor. CONCLUSION: The progress across various disciplines may have contributed to improved OS and DSS in this study compared to previous reports.

19.
Bone Joint Res ; 12(12): 712-721, 2023 Dec 04.
Artículo en Inglés | MEDLINE | ID: mdl-38043570

RESUMEN

Aims: Research on hip biomechanics has analyzed femoroacetabular contact pressures and forces in distinct hip conditions, with different procedures, and used diverse loading and testing conditions. The aim of this scoping review was to identify and summarize the available evidence in the literature for hip contact pressures and force in cadaver and in vivo studies, and how joint loading, labral status, and femoral and acetabular morphology can affect these biomechanical parameters. Methods: We used the PRISMA extension for scoping reviews for this literature search in three databases. After screening, 16 studies were included for the final analysis. Results: The studies assessed different hip conditions like labrum status, the biomechanical effect of the cam, femoral version, acetabular coverage, and the effect of rim trimming. The testing and loading conditions were also quite diverse, and this disparity limits direct comparisons between the different researches. With normal anatomy the mean contact pressures ranged from 1.54 to 4.4 MPa, and the average peak contact pressures ranged from 2 to 9.3 MPa. Labral tear or resection showed an increase in contact pressures that diminished after repair or reconstruction of the labrum. Complete cam resection also decreased the contact pressure, and acetabular rim resection of 6 mm increased the contact pressure at the acetabular base. Conclusion: To date there is no standardized methodology to access hip contact biomechanics in hip arthroscopy, or with the preservation of the periarticular soft-tissues. A tendency towards improved biomechanics (lower contact pressures) was seen with labral repair and reconstruction techniques as well as with cam correction.

20.
Trauma Case Rep ; 43: 100769, 2023 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-36686410

RESUMEN

Bilateral sacroiliac joint dislocation is a rare injury associated with rotational and vertical instability of the pelvic ring. The ideal strategy for the reduction and fixation of this injury is poorly described in the current literature. Triangular osteosynthesis provides multiplanar stability to the posterior pelvic ring allowing early weight bearing. We present the case of a young female with a bilateral sacroiliac joint dislocation who underwent a modified bilateral triangular osteosynthesis, using S1 pedicle screws to improve the reduction of the sacroiliac joint.

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