Genome-wide association study identifies novel susceptibilities to adult moyamoya disease.

Genome-wide association study has limited to discover single-nucleotide polymorphisms (SNPs) in several ethnicities. Here, we investigated an initial GWAS to identify genetic modifiers predicting with adult moyamoya disease (MMD) in Koreans. GWAS was performed in 216 patients with MMD and 296 controls using the large-scale Asian-specific Axiom Precision Medicine Research Array. A subsequent fine-mapping analysis was conducted to assess the causal variants associated with adult MMD. A total of 489,966 out of 802,688 SNPs were subjected to quality control analysis. Twenty-one SNPs reached a genome-wide significance threshold (p = 5 × 10-8) after pruning linkage disequilibrium (r2 < 0.8) and mis-clustered SNPs. Among these variants, the 17q25.3 region including TBC1D16, CCDC40, GAA, RNF213, and ENDOV genes was broadly associated with MMD (p = 3.1 × 10-20 to 4.2 × 10-8). Mutations in RNF213 including rs8082521 (Q1133K), rs10782008 (V1195M), rs9913636 (E1272Q), rs8074015 (D1331G), and rs9674961 (S2334N) showed a genome-wide significance (1.9 × 10-8 < p < 4.3 × 10-12) and were also replicated in the East-Asian populations. In subsequent analysis, RNF213 mutations were validated in a fine-mapping outcome (log10BF > 7). Most of the loci associated with MMD including 17q25.3 regions were detected with a statistical power greater than 80%. This study identifies several novel and known variations predicting adult MMD in Koreans. These findings may good biomarkers to evaluate MMD susceptibility and its clinical outcomes.

Patient experience and prognostic factors of compensatory hyperhidrosis and recurrence after endoscopic thoracic sympathicotomy.

OBJECTIVE: We aimed to investigate compensatory hyperhidrosis (CH) and recurrence based on an online survey of patients who underwent endoscopic thoracic sympathicotomy(ETS) for palmar and/or axillary hyperhidrosis. METHODS: We enrolled 231 patients who underwent ETS for palmar and/or axillary hyperhidrosis from January 2008 to April 2021. Patients responded to an online questionnaire regarding CH and recurrence, their electronic medical records were reviewed. Logistic regression was performed to find the risk factors related to CH and recurrence. RESULTS: The median time of survey from surgery was 20 months. Although 94% of patients were satisfied with the surgery, 86.1% experienced CH; of them, it was severe in 30.7%. Three months after surgery, there was no long-term change in the severity of CH. The development of CH showed a close relationship with age of 20 years or more (OR: 2.73). Recurrence occurred in 44(19.0%) patients, and the use of anti-adhesive agents was a significant preventive factor against recurrence after ETS (OR: 0.42). CONCLUSIONS: We observed that CH and recurrence after ETS for palmar and/or axillary hyperhidrosis were relatively common. Age at the time of surgery was associated with CH, and the use of anti-adhesive agents showed to lower the risk of recurrence after ETS.

Dual-Mode Radar Sensor for Indoor Environment Mapping.

In this paper, we introduce mapping results in an indoor environment based on our own developed dual-mode radar sensor. Our radar system uses a frequency-modulated continuous wave (FMCW) with a center frequency of 62 GHz and a multiple-input multiple-output antenna system. In addition, the FMCW radar sensor we designed is capable of dual-mode detection, which alternately transmits two waveforms using different bandwidths within one frame. The first waveform is for long-range detection, and the second waveform is for short-range detection. This radar system is mounted on a small robot that moves in indoor environments such as rooms or hallways, and the radar and the robot send and receive necessary information to each other. The radar estimates the distance, velocity, and angle information of targets around the radar-equipped robot. Then, the radar receives information about the robot's motion from the robot, such as its speed and rotation angle. Finally, by combining the motion information and the detection results, the radar-equipped robot maps the indoor environment while finding its own position. Compared to the actual map data, the radar-based mapping is effectively achieved through the radar system we developed.

Extracellular Mitochondrial Dysfunction in Cerebrospinal Fluid of Patients with Delayed Cerebral Ischemia after Aneurysmal Subarachnoid Hemorrhage.

BACKGROUND: Mitochondrial dysfunction is related to brain ischemic injury and neural cell death. However, little is known about the association between mitochondrial dysfunction of cerebrospinal fluid (CSF) and delayed cerebral ischemia (DCI) following subarachnoid hemorrhage (SAH). The objective of this study was to investigate whether extracellular CSF mitochondria might serve as a potential biomarker for DCI. METHODS: CSF samples were serially collected at 1, 3, and 5 days following SAH in 33 patients (DCI, n = 12; and non-DCI, n = 21) who underwent coil embolization. To monitor mitochondrial membrane potentials, JC-1 dye was used. The ratio (red/green) of JC-1 was considered as an indicator of intact mitochondrial membrane potential. Flow cytometry was done to analyze extracellular mitochondria particles and their possible cellular origins. RESULTS: DCI patients had lower JC-1 red/green ratios than non-DCI patients at 1 day (3.35 [3.20-3.75] vs. 3.70 [3.40-3.95] in non-DCI) and 3 days (4.65 [4.45-5.00] vs. 5.10 [4.65-5.30] in non-DCI) after SAH. At 5 days after SAH, JC-1 red/green ratio was significantly lower in DCI than that in non-DCI (3.05 [2.90-3.35] vs. 4.20 [4.10-4.50]; p < 0.01) patients. DCI patients had a higher percentage of vWF-positive mitochondria (40.10% [38.25%-44.90%] vs. 30.20% [25.70%-36.68%]) and a lower percentage of GLAST-positive mitochondria particles (26.85% [17.10%-30.00%] vs. 31.60% [26.70%-35.00%]) than non-DCI patients. However, there was no significant difference in CD45-positive (p = 0.369) or CD41/61-positive mitochondrial particles (p = 0.155) between the two groups of patients. CONCLUSIONS: Mitochondrial membrane potential could be a marker of DCI. JC-1 ratios seemed to be able to predict future DCI onset. Further studies are needed to determine detailed mechanisms of extracellular mitochondria-mediated cell-to-cell signals in DCI.

Characterization of the TCR ß Chain CDR3 Repertoire in Subarachnoid Hemorrhage Patients with Delayed Cerebral Ischemia.

Little is known of the adaptive immune response to subarachnoid hemorrhage (SAH). This study was the first to investigate whether T cell receptor (TCR) immune repertoire may provide a better understanding of T cell immunology in delayed cerebral ischemia (DCI). We serially collected peripheral blood in five SAH patients with DCI. High-throughput sequencing was used to analyze the TCR ß chain (TCRB) complimentary determining regions (CDR) 3 repertoire. We evaluated the compositions and variations of the repertoire between admission and the DCI period, for severe DCI and non-severe DCI patients. Clonality did not differ significantly between admission and DCI. Severe DCI patients had significantly lower clonality than non-severe DCI patients (p value = 0.019). A read frequency of 0.005% ≤ - < 0.05% dominated the clonal expansion in non-severe DCI patients. Regarding repertoire diversity, severe DCI had a higher diversity score on admission than non-severe DCI. The CDR3 lengths were similar between admission and DCI. Among 728 annotated V-J gene pairs, we found that the relative frequencies of two V-J pairs were different at the occurrence of DCI than at admission, with T cells increasing by over 15%. TCRB CDR3 repertoires may serve as biomarkers to identify severe DCI patients.

Synthesis and gas sensing properties of WS2 nanocrystallites assembled hierarchical WS2 fibers by electrospinning.

A gas sensor based on a hierarchical WS2 structure embedded with vertically aligned WS2 nanocrystallites was demonstrated. The three-dimensional (3D) hierarchical structure provides many edge sites of nanocrystallites and an extremely large gas contact volume, resulting in a high gas response. The decreased contact resistance between the 3D hierarchical WS2 fibers and sensor electrode resulted in improved NO2 response. We fabricated a one-dimensional (1D) conductive WS2 fiber using a two-step annealing process under sulfur flow (sulfurization). It delivers a continuous and conductive carrier path and lowers the potential barrier at the interface of the WS2 nanocrystallites (top) and electrospun WS2 fiber (bottom), resulting in an improved gas response. We developed 3D hierarchical WS2 fibers embedded with vertically aligned WS2 nanocrystallites to increase the gas adsorption site in comparison with that of 1D WS2 fibers without WS2 flakes. Vertically aligned WS2 nanocrystallites were formed after a two-step annealing treatment. Sensors based on the 3D hierarchical WS2 fibers embedded with WS2 flakes, showed higher response to NO2 gas in comparison to that of pure WS2 fibers without WS2 flakes.

60 mA Bidirectional Current Gating in Vanadium-Dioxide-Based Planar Device Using CO2 Laser.

Here we show 60 mA bidirectional current gating in a two-terminal planar device based on a highly resistive vanadium dioxide (VO2) thin film by harnessing photothermally induced phase transition occurred in VO2 when irradiating the VO2 film with a CO2 laser oscillating at 10.6 µm. The VO2 thin film was grown by a pulsed laser deposition method, and the two-terminal planar device was fabricated using the VO2 film isolated with sub-millimeter dimensions. The bidirectional current gating between 0 and 60 mA was accomplished by irradiating the VO2-based device with repetitive pulses of the CO2 laser. In terms of laser modulation parameters such as the pulse width and repetition rate, their effect on the transient responses of laser-gated currents was also investigated. With a minimum energy per pulse of ~766 mJ, a stable bidirectional current gating of up to 60 mA could be successfully implemented for the repetition rates of 0.5-3.0 Hz in a VO2 device biased at ~5.4 V, showing a switching contrast between off- and on-state currents of ~11089. This maximum onstate current (60 mA) and switching contrast are the highest values among previous gating results attained in VO2 devices with a CO2 laser.

Laser-Regulated 60 mA Current Switching in VO2-Based Two-Terminal Device Using 976 nm Laser Diode.

By incorporating a high-power 976 nm laser diode (LD), we demonstrated laser-regulated current switching in a two-terminal planar device based on a vanadium dioxide (VO2) thin film. The VO2 thin film was grown by pulsed laser deposition method and etched to sub-millimeter dimension for the fabrication of a two-terminal device. The reversible current switching was implemented by controlling the on/off state of the LD, which illuminates the VO2-based device. The transient responses of the device currents were analyzed when the device was excited with laser pulses of various repetition rates of up to 5.0 Hz with a pulse width fixed as 75 ms. A switching contrast between off- and on-state currents was calculated as ~9530, and average rising and falling times were measured as ~31 and ~21 ms, respectively.

Osteoconductivity of Binary Titanium Alloys with Different Micro/Nanoporous Surfaces.

The surface characteristics and osteoconductivity were evaluated for the micro/nanoporous surfaces of titanium (Ti) alloys prepared by micro-arc oxidation (MAO) and hydrothermal treatment (HT) of binary Ti-5 wt% A alloys (A = Au, Mn, Nb, and Pd). Surface properties were analyzed using X-ray diffractometry, scanning electron microscopy, and energy dispersive X-ray spectroscopy. The osteoconductivity was evaluated by measuring the total protein, ALPase activity, and osteocalcin production. The surface morphologies of MAO/HT specimens mainly affected on their osteoconductivity. Total proteins on Ti alloys (MAO/HT) were slightly lower than that on commercially pure Ti (MAO/HT) after incubation of MG-63 osteoblast-like cells for 14 days. However, better ALPase activity and osteocalcin production were observed on MAO/HT-treated Ti­5Mn, Ti­5Nb, and Ti­5Pd than that on cp-Ti (MAO/HT) after 14 days. Especially, Ti­5Mn (MAO/HT) showed a significant increase of ALPase activity due to its well grown micro/nano structure. Meanwhile, very small nanorods on Ti­5Au (MAO/HT) affected negatively to ALPase activity and osteocalcin production.

Bidirectional current triggering in planar devices based on serially connected VO2 thin films using 965 nm laser diode.

By incorporating a 965 nm laser diode, the bidirectional current triggering of up to 30 mA was demonstrated in a two-terminal planar device based on serially connected vanadium dioxide (VO2) thin films grown by pulsed laser deposition. The bidirectional current triggering was realized by using the focused beams of laser pulses through the photo-thermally induced phase transition of VO2. The transient responses of laser-triggered currents were also investigated when laser pulses excited the device at a variety of pulse widths and repetition rates of up to 4.0 Hz. A switching contrast between off- and on-state currents was obtained as ~8333, and rising and falling times were measured as ~39 and ~29 ms, respectively, for 50 ms laser pulses.

Strong Optical Dipole Force Exerted on Molecules Having Low Rotational Temperature.

The optical dipole force acting on molecules is enhanced by decreasing the rotational temperature of the molecule and aligning the molecular axis with a linearly polarized nonresonant laser beam. The rotational temperature is decreased by increasing the source pressure from 2 to 81 bar. By using the effective polarizability directly pertaining to the optical dipole force, the force and the resulting change in the velocity of the molecules can be evaluated. Theoretical calculations are compared with measurements based on velocity map imaging techniques. If the rotational temperature is reduced from 295 to 1 K, the maximum alignment is increased from =0.33 to 0.92, and the average optical force is enhanced by 74 %.

Universal Diffraction of Atomic and Molecular Matter-Waves: A Comparison of He and D2 Quantum Reflected from a Grating.

Molecular beams of He and D2 are scattered from a ruled diffraction grating in conical-mount geometry under grazing-incidence conditions. Fully resolved diffraction patterns as a function of detection angle are recorded for different grating azimuth angles and for two different kinetic energies of the particle beams. Variations in diffraction peak widths are traced back to different velocity spreads of He and D2 determined by time-of-flight measurements. A comprehensive analysis of diffraction intensities confirms universal diffraction, that is, for identical de Broglie wavelengths, the relative diffraction intensities for He and D2 are the same. Universal diffraction results from peculiarities of quantum reflection of the atoms and molecules from the diffraction grating. In quantum reflection particles scatter many nanometers in front of the surface from the long-range attractive branch of the particle-surface interaction potential without probing the potential well and the short-range repulsive branch of the potential.

A Vanadium Dioxide Metamaterial Disengaged from Insulator-to-Metal Transition.

We report that vanadium dioxide films patterned with λ/100000 nanogaps exhibit an anomalous transition behavior at millimeter wavelengths. Most of the hybrid structure's switching actions occur well below the insulator to metal transition temperature, starting from 25 °C, so that the hysteresis curves completely separate themselves from their bare film counterparts. It is found that thermally excited intrinsic carriers are responsible for this behavior by introducing enough loss in the context of the radically modified electromagnetic environment in the vicinity of the nanogaps. This phenomenon newly extends the versatility of insulator to metal transition devices to encompass their semiconductor properties.

20 mA bidirectional laser triggering in planar devices based on vanadium dioxide thin films using CO(2) laser.

By utilizing a CO2 laser centered at ~10.6 µm as an optical stimulus, we demonstrated bidirectional laser triggering in a two-terminal planar device based on a highly resistive vanadium dioxide (VO2) thin film. The break-over voltage of the VO2-based device was measured as large as ~294.8 V, which resulted from the high resistivity of insulating VO2 grains comprising the thin film and the large electrode separation of the device. The bidirectional current switching of up to 20 mA was achieved by harnessing the dramatic resistance variation of the device photo-thermally induced by the laser illumination. The transient responses of laser-triggered currents were also analyzed when laser pulses excited the device at a variety of pulse widths and repetition rates. In the transient responses, a maximum switching contrast between off- and on-state currents was measured as ~7067 with an off-state current of ~2.83 µA, and rising and falling times were measured as ~30 and ~16 ms, respectively, for 100 ms laser pulses.

Bidirectional laser triggering of planar device based on vanadium dioxide thin film.

By incorporating a 1550 nm laser diode, bidirectional laser triggering was investigated in a two-terminal planar device based on vanadium dioxide (VO2) thin film grown by sol-gel method. A specific bias voltage range enabling the bidirectional laser triggering was experimentally found from the current-voltage characteristics of the VO2-based device, which was measured in a current-controlled mode. At a bias voltage selected within the range, 10 mA bidirectional triggering was implemented with a maximum amplitude switching ratio of ~68.2, and the transient responses of light-triggered currents were also analyzed.

Shore hardness and tensile bond strength of long-term soft denture lining materials.

STATEMENT OF PROBLEM: Reduced softness and separation from the denture base are the most significant problems of long-term soft lining materials. PURPOSE: The purpose of this study was to evaluate the durometer Shore A hardness and tensile bond strength of long-term soft denture lining materials and to investigate the correlation between these 2 properties. MATERIAL AND METHODS: A group of 7 soft lining materials, 6 silicone based (Dentusil, GC Reline Soft, GC Reline Ultrasoft, Mucopren Soft, Mucosoft, Sofreliner Tough) and 1 acrylic resin based (Durabase), were evaluated for durometer Shore A hardness and tensile bond strength to heat-polymerized denture base resin (Lucitone 199). A specially designed split mold and loading assembly with a swivel connector were used for the durometer Shore A hardness test and tensile bond strength test to improve accuracy and facilitate measurement. Three specimens of each product were stored in a 37°C water bath, and durometer Shore A hardness tests were carried out after 24 hours and 28 days. A tensile bond strength test was carried out for 10 specimens of each product, which were stored in a 37°C water bath for 24 hours before the test. Repeated-measures ANOVA, the Kruskal-Wallis and Duncan multiple range tests, and the Spearman correlation were used for statistical analyses. RESULTS: The repeated-measures ANOVA found significant durometer Shore A hardness differences for the materials (P<.001) and the interaction effect (aging×materials) (P<.001). GC Reline Ultrasoft showed the lowest mean durometer Shore A hardness (21.30 ±0.29 for 24 hours, 34.73 ±0.47 for 28 days), and GC Reline Soft showed the highest mean durometer Shore A hardness (50.13 ±0.48 for 24 hours, 57.20 ±0.28 for 28 days). The Kruskal-Wallis test found a significant difference in the mean tensile bond strength values (P<.001). GC Reline Ultrasoft (0.82 ±0.32 MPa) and Mucopren Soft (0.96 ±0.46 MPa) had a significantly lower mean tensile bond strength (P<.05). GC Reline Soft had the highest mean tensile bond strength (2.99 ±0.43 MPa) (P<.05), and acrylic resin-based Durabase showed a significantly different tensile bond strength (1.32 ±0.16 MPa), except for Mucopren Soft, among the materials (P<.05). The tensile bond strength and Shore A hardness showed a statistically insignificant moderate positive correlation (r=0.571, P=.180 for Shore A hardness 24 hours versus tensile bond strength; r=0.607, P=.148 for Shore A hardness 28 days versus tensile bond strength). CONCLUSIONS: Within the limitations of this study, significant differences were found in durometer Shore A hardness (with aging time) and tensile bond strength among the materials. Adhesive failure was moderately correlated with durometer Shore A hardness, especially after 28 days, but was not significant.

Machine learning approach for carbon disclosure in the Korean market: The role of environmental performance.

Over the past few decades, scholars have employed a wide range of methodologies to determine the factors influencing firms' voluntary carbon disclosure. Most of these studies have been conducted in advanced markets. This article aims to examine the trend of voluntary carbon disclosure in the Korean financial market by utilizing machine learning models such as Random Forest and Gradient Boosted Decision Tree. Based on a set of hand-collected carbon disclosure data, we initially demonstrated significantly better performance of machine learning models compared to the traditional logistic model. Regarding the factors influencing disclosure, we consistently find the importance of environmental scores, emphasizing the role of the emerging mega-trend of ESG management practices in disclosure decisions. However, in contrast to recent studies, we do not find that the unique Korean governance structure, chaebol, has any significantly different implications in terms of prediction performance and variable importance in carbon disclosure decisions.

reliable Synthesis of Monodisperse Microparticles: Prevention of Oxygen Diffusion and Organic Solvents Using Conformal Polymeric Coating onto Poly(dimethylsiloxane) Micromold.

An effective polymeric thin film deposited by initiated chemical vapor deposition (iCVD) process was presented and its application as a barrier film on the PDMS micromold blocking the penetration of oxygen and organic solvents was investigated. With this barrier film, we were able to synthesize monodisperse polymeric particles of sizes down to 3 µm, which has been reported to be extremely challenging with bare PDMS micromold. The polymeric barrier film on the PDMS micromold enabled this successful synthesis of microparticles by effectively blocking the diffusion of oxygen, which is a well-known radical quencher in radical polymerization, through the PDMS micromold. Furthermore, the iCVD barrier film substantially decreased the penetration of various organic solvents such as acetone, tert-butanol, PDMS oil, and decane as well as organic substances including fluorescent molecules like rhodamine B and fluorescein isothiocyanate (FITC). Therefore, the polymeric barrier film coated on PDMS micromold via iCVD process will broaden the application of PDMS to microfluidic area for the synthesis of smaller microparticles with various organic substances.

Oxidized Regenerated Cellulose versus Polyglycolic Acid for Pleural Coverage in Pneumothorax Surgery.

Objectives: Although surgical intervention for spontaneous pneumothorax (SP) reduces the recurrence rate, thoracoscopic surgery is associated with greater postoperative recurrence rates than open thoracotomy. A polyglycolic acid (PGA) sheet or oxidized regenerated cellulose (ORC) mesh can therefore be used for additional coverage after thoracoscopic surgery, and this study compared the clinical impacts of these two materials. Methods: From 2018 to 2020, 262 thoracoscopic surgeries for primary SP were performed, of which 125 patients were enrolled in this study, and 48 and 77 patients received ORC and PGA coverage, respectively. The clinical characteristics and surgical procedures were reviewed, and the recurrence rates were compared. To obtain more comprehensive evidence, we performed a literature review and meta-analysis comparing ORC and PGA coverage. Results: There were no significant differences in patient characteristics between the two groups. Operating time was slightly shorter in the ORC group than in the PGA group (p = 0.008). The pneumothorax recurrence rate was similar in both groups (PGA: 10.4%, ORC: 6.2%, p = 0.529), but the recurrence-free interval was significantly longer (p = 0.036) in the ORC (262 days) than in the PGA (48.5 days) group. The literature review identified three relevant studies, and the meta-analysis revealed no difference in pneumothorax recurrence rate between the two coverage materials. Conclusions: The two visceral pleural coverage materials, PGA and ORC, did not show significant differences in postoperative pneumothorax recurrence. Therefore, if applied appropriately, the choice of material between ORC and PGA for thoracoscopic pneumothorax surgery does not have a significant impact on the clinical outcome.

Proposal of a revised International Association for the Study of Lung Cancer grading system in pulmonary non-mucinous adenocarcinoma: The importance of the lepidic proportion.

OBJECTIVES: We aimed to measure the validity of the International Association for the Study of Lung Cancer (IASLC) grading system in Korean patients and propose a modification for an increase of its predictability, especially in grade 2 patients. MATERIALS AND METHODS: From 2012 to 2017, histopathologic characteristics of 1358 patients with invasive pulmonary adenocarcinoma (stage I-III) from two institutions were retrospectively reviewed and re-classified according to the IASLC grading system. Considering the amount of the lepidic proportion, the validity of the revised model (Lepidic-10), derived from the training cohort (hospital A), was measured using the validation cohort (hospital B). Its predictability was compared to that of the IASLC system. RESULTS: Of the 1358 patients, 259 had a recurrence, and 189 died during follow-up. The Harrell's concordance index and area under the curve of the IASLC system were 0.685 and 0.699 for recurrence-free survival (RFS) and 0.669 and 0.679 for death, respectively. From the training cohort, the IASLC grade 2 patients were divided into grades 2a and 2b (Lepidic-10 model) with a 10 % lepidic pattern. This new model further distinguished patients in both institutions that had better performance than the IASLC grading (Hospital A, p < 0.001 for RFS and death; Hospital B, p = 0.0215 for RFS, p = 0.0429 for death). CONCLUSION: The IASLC grading system was easily applicable; its clinical use in predicting the prognosis of Korean patients with pulmonary adenocarcinoma was validated. Furthermore, the introduction of the lepidic proportion as an additional criterion to differentiate grade 2 patients improved its predictability.