Safe and just Earth system boundaries.

The stability and resilience of the Earth system and human well-being are inseparably linked1-3, yet their interdependencies are generally under-recognized; consequently, they are often treated independently4,5. Here, we use modelling and literature assessment to quantify safe and just Earth system boundaries (ESBs) for climate, the biosphere, water and nutrient cycles, and aerosols at global and subglobal scales. We propose ESBs for maintaining the resilience and stability of the Earth system (safe ESBs) and minimizing exposure to significant harm to humans from Earth system change (a necessary but not sufficient condition for justice)4. The stricter of the safe or just boundaries sets the integrated safe and just ESB. Our findings show that justice considerations constrain the integrated ESBs more than safety considerations for climate and atmospheric aerosol loading. Seven of eight globally quantified safe and just ESBs and at least two regional safe and just ESBs in over half of global land area are already exceeded. We propose that our assessment provides a quantitative foundation for safeguarding the global commons for all people now and into the future.

Dietary shifts may underpin the recovery of a large carnivore population.

Supporting the recovery of large carnivores is a popular yet challenging endeavour. Estuarine crocodiles in Australia are a large carnivore conservation success story, with the population having extensively recovered from past heavy exploitation. Here, we explored if dietary changes had accompanied this large population recovery by comparing the isotopes δ13C and δ15N in bones of crocodiles sampled 40 to 55 years ago (small population) with bones from contemporary individuals (large population). We found that δ13C and δ15N values were significantly lower in contemporary crocodiles than in the historical cohort, inferring a shift in prey preference away from marine and into terrestrial food webs. We propose that an increase in intraspecific competition within the recovering crocodile population, alongside an increased abundance of feral ungulates occupying the floodplains, may have resulted in the crocodile population shifting to feed predominantly upon terrestrial food sources. The number of feral pigs consumed to sustain and grow crocodile biomass may help suppress pig population growth and increase the flow of terrestrially derived nutrients into aquatic ecosystems. The study highlights the significance of prey availability in contributing to large carnivore population recovery.

Longitudinal variation in the nutritional quality of basal food sources and its effect on invertebrates and fish in subalpine rivers.

There is growing recognition of the importance of food quality over quantity for aquatic consumers. In streams and rivers, most previous studies considered this primarily in terms of the quality of terrestrial leaf litter and importance of microbial conditioning. However, many recent studies suggest that algae are a more nutritional food source for riverine consumers than leaf litter. To date, few studies have quantified longitudinal shifts in the nutritional quality of basal food resources in river ecosystems and how these may affect consumers. We conducted a field investigation in a subalpine river ecosystem in Austria to investigate longitudinal variations in diet quality of basal food sources (submerged leaves and periphyton) and diet source dependence of stream consumers (invertebrate grazers, shredders, filterers and predators, and fish). Fatty acid (FA) profiles of basal food sources and their consumers were measured. Our results indicate systematic differences between the FA profiles of terrestrial leaves and aquatic biota, that is periphyton, invertebrates and fish. Submerged leaves contained very low proportions of long-chain polyunsaturated fatty acids (LC-PUFAs), which were conversely rich in aquatic biota. While the FA composition of submerged leaves remained similar among sites, the LC-PUFAs of periphyton increased longitudinally, which was associated with increasing nutrients from upstream to downstream. Longitudinal variations in periphyton LC-PUFAs were reflected in the LC-PUFAs of invertebrate grazers and shredders, and further tracked by invertebrate predators and fish. However, brown trout Salmo trutta contained a large proportion of docosahexaenoic acid (DHA, 22:6ω3), a LC-PUFA almost entirely missing in basal sources and invertebrates. The fish accumulated eicosapentaenoic acid (EPA, 20:5ω3) from invertebrate prey and may use this FA to synthesize DHA. Our results provide a nutritional perspective for river food web studies, emphasizing the importance of algal resources to consumer somatic growth and the need to account for the longitudinal shifts in the quality of these basal resources.

Bending the Curve of Global Freshwater Biodiversity Loss: An Emergency Recovery Plan.

Despite their limited spatial extent, freshwater ecosystems host remarkable biodiversity, including one-third of all vertebrate species. This biodiversity is declining dramatically: Globally, wetlands are vanishing three times faster than forests, and freshwater vertebrate populations have fallen more than twice as steeply as terrestrial or marine populations. Threats to freshwater biodiversity are well documented but coordinated action to reverse the decline is lacking. We present an Emergency Recovery Plan to bend the curve of freshwater biodiversity loss. Priority actions include accelerating implementation of environmental flows; improving water quality; protecting and restoring critical habitats; managing the exploitation of freshwater ecosystem resources, especially species and riverine aggregates; preventing and controlling nonnative species invasions; and safeguarding and restoring river connectivity. We recommend adjustments to targets and indicators for the Convention on Biological Diversity and the Sustainable Development Goals and roles for national and international state and nonstate actors.

Strong evidence for changing fish reproductive phenology under climate warming on the Tibetan Plateau.

Phenological responses to climate change have been widely observed and have profound and lasting effects on ecosystems and biodiversity. However, compared to terrestrial ecosystems, the long-term effects of climate change on species' phenology are poorly understood in aquatic ecosystems. Understanding the long-term changes in fish reproductive phenology is essential for predicting population dynamics and for informing management strategies, but is currently hampered by the requirement for intensive field observations and larval identification. In this study, a very low-frequency sampling of juveniles and adults combined with otolith measurements (long axis length of the first annulus; LAFA) of an endemic Tibetan Plateau fish (Gymnocypris selincuoensis) was used to examine changes in reproductive phenology associated with climate changes from the 1970s to 2000s. Assigning individual fish to their appropriate calendar year class was assisted by dendrochronological methods (crossdating). The results demonstrated that LAFA was significantly and positively associated with temperature and growing season length. To separate the effects of temperature and the growing season length on LAFA growth, measurements of larval otoliths from different sites were conducted and revealed that daily increment additions were the main contributor (46.3%), while temperature contributed less (12.0%). Using constructed water-air temperature relationships and historical air temperature records, we found that the reproductive phenology of G. selincuoensis was strongly advanced in the spring during the 1970s and 1990s, while the increased growing season length in the 2000s was mainly due to a delayed onset of winter. The reproductive phenology of G. selincuoensis advanced 2.9 days per decade on average from the 1970s to 2000s, and may have effects on recruitment success and population dynamics of this species and other biota in the ecosystem via the food web. The methods used in this study are applicable for studying reproductive phenological changes across a wide range of species and ecosystems.

Feeding strategies for the acquisition of high-quality food sources in stream macroinvertebrates: Collecting, integrating, and mixed feeding.

Aquatic macroinvertebrates play an important functional role in energy transfer in food webs, linking basal food sources to upper trophic levels that include fish, birds, and humans. However, the trophic coupling of nutritional quality between macroinvertebrates and their food sources is still poorly understood. We conducted a field study in subalpine streams in Austria to investigate how the nutritional quality (measured by long-chain polyunsaturated fatty acids, LC-PUFAs) in macroinvertebrates changes relative to their basal food sources. Samples of macroinvertebrates, periphyton, and leaves were collected from 17 streams in July and October 2016 and their fatty acid (FA) composition was analyzed. Periphyton FA varied strongly with time and space, and their trophic effect on macroinvertebrate FA differed among functional feeding groups. The match between periphyton FA and macroinvertebrate FA decreased with increasing trophic levels, but LC-PUFA content increased with each trophic step from periphyton to grazers and finally predators. Macroinvertebrates fed selectively on, assimilated, and/or actively controlled their LC-PUFA, especially eicosapentaenoic acid (EPA, 20 : 5ω3) relative to their basal food sources in the face of spatial and temporal changes. Grazer FA profiles reflected periphyton FA with relatively good fidelity, and especially their EPA feeding strategy was primarily linked to periphyton FA variation across seasons. In contrast, shredders appeared to preferentially assimilate more EPA over other FA, which was determined by the availability of high-quality food over seasons. Predators may more actively control their LC-PUFA distribution with respect to different quality foods and showed less fidelity to the basal FA profiles in plants and prey. Overall, grazers and shredders showed relatively good fidelity to food FA profiles and performed as both "collectors" and "integrators" for LC-PUFA requirements across seasons, while predators at higher trophic levels were more "integrators" with added metabolic complexity leading to somewhat more divergent FA profiles. These results are potentially applicable for other aquatic consumers in freshwater and marine ecosystems.

Quantifying learning in biotracer studies.

Mixing models have become requisite tools for analyzing biotracer data, most commonly stable isotope ratios, to infer dietary contributions of multiple sources to a consumer. However, Bayesian mixing models will always return a result that defaults to their priors if the data poorly resolve the source contributions, and thus, their interpretation requires caution. We describe an application of information theory to quantify how much has been learned about a consumer's diet from new biotracer data. We apply the approach to two example data sets. We find that variation in the isotope ratios of sources limits the precision of estimates for the consumer's diet, even with a large number of consumer samples. Thus, the approach which we describe is a type of power analysis that uses a priori simulations to find an optimal sample size. Biotracer data are fundamentally limited in their ability to discriminate consumer diets. We suggest that other types of data, such as gut content analysis, must be used as prior information in model fitting, to improve model learning about the consumer's diet. Information theory may also be used to identify optimal sampling protocols in situations where sampling of consumers is limited due to expense or ethical concerns.

Body size drives allochthony in food webs of tropical rivers.

Food web subsidies from external sources ("allochthony") can support rich biological diversity and high secondary and tertiary production in aquatic systems, even those with low rates of primary production. However, animals vary in their degree of dependence on these subsidies. We examined dietary sources for aquatic animals restricted to refugial habitats (waterholes) during the dry season in Australia's wet-dry tropics, and show that allochthony is strongly size dependent. While small-bodied fishes and invertebrates derived a large proportion of their diet from autochthonous sources within the waterhole (phytoplankton, periphyton, or macrophytes), larger animals, including predatory fishes and crocodiles, demonstrated allochthony from seasonally inundated floodplains, coastal zones or the surrounding savanna. Autochthony declined roughly 10% for each order of magnitude increase in body size. The largest animals in the food web, estuarine crocodiles (Crocodylus porosus), derived ~80% of their diet from allochthonous sources. Allochthony enables crocodiles and large predatory fish to achieve high biomass, countering empirically derived expectations for negative density vs. body size relationships. These results highlight the strong degree of connectivity that exists between rivers and their floodplains in systems largely unaffected by river regulation or dams and levees, and how large iconic predators could be disproportionately affected by these human activities.

Effects of light and nutrients on periphyton and the fatty acid composition and somatic growth of invertebrate grazers in subtropical streams.

Algal polyunsaturated fatty acids (PUFA), essential for somatic growth and reproduction of aquatic animals, are influenced by ambient environmental conditions, including light and nutrients. Few studies have addressed the extent to which changes in algal PUFA can influence stream herbivore PUFA profiles and the implications for stream food webs. We manipulated subtropical stream periphyton by applying two light levels (open and shaded canopy) and two nutrient regimes (ambient and enriched) to investigate the response of PUFA and somatic growth in stream herbivores. After 6 weeks, the relative content of periphyton PUFA (%) changed distinctly and differed among treatments. Periphyton in the control treatment with open canopy showed a decline in eicosapentaenoic acid (EPA) relative to initial conditions, whereas shading increased EPA and total highly unsaturated FA (HUFA), but decreased α-linolenic acid (ALA), linoleic acid and total C18 PUFA. The interaction of open canopy and added nutrients increased periphyton ALA compared with initial conditions, while the combined effects of shading and added nutrients led to greater total HUFA. FA similarity between stream grazers (the mayfly Austrophlebioides and caddisfly Helicopsyche) and periphyton increased with periphyton HUFA content. In addition, the growth of large instars of both grazers also increased in response to increased periphyton HUFA %. Our findings show that environmental changes, associated with riparian canopy and nutrients, can lead to changes in periphyton PUFA composition that in turn affect growth and PUFA composition in stream grazers.

Does flood rhythm drive ecosystem responses in tropical riverscapes?

Biotic communities are shaped by adaptations from generations of exposure to selective pressures by recurrent and often infrequent events. In large rivers, floods can act as significant agents of change, causing considerable physical and biotic disturbance while often enhancing productivity and diversity. We show that the relative balance between these seemingly divergent outcomes can be explained by the rhythmicity, or predictability of the timing and magnitude, of flood events. By analyzing biological data for large rivers that span a gradient of rhythmicity in the Neotropics and tropical Australia, we find that systems with rhythmic annual floods have higher-fish species richness, more stable avian populations, and elevated rates of riparian forest production compared with those with arrhythmic flood pulses. Intensification of the hydrological cycle driven by climate change, coupled with reductions in runoff due to water extractions for human use and altered discharge from impoundments, is expected to alter the hydrologic rhythmicity of floodplain rivers with significant consequences for both biodiversity and productivity.

Development of a benthic diatom index of biotic integrity (BD-IBI) for ecosystem health assessment of human dominant subtropical rivers, China.

As efforts intensify to address the issues of declining water quality and biodiversity losses in freshwater ecosystems, there have been great demands for effective methods of evaluating aquatic ecosystem health. In this study, benthic algae assemblages and water quality variables were analyzed to develop a benthic diatom-based index of biotic integrity (BD-IBI) for assessment of the aquatic environment in the upper Han River (China). Through the use of multivariate and multimetric approaches, four metrics - % prostrate individuals, % Amphora individuals, % polysaprob species, and diatom-based eutrophication/pollution index (EPI-D) - were identified from 98 candidate metrics to develop a BD-IBI. Application of the index revealed that water quality in 11% of the 31 sampled sites could be described as excellent condition, in 43% of the sites it could be described as good condition, in 25% as moderate condition, and in 21% as poor condition. The assessment further revealed that the main reason for degradation of the Han river ecosystem was nutrient enrichment through agricultural land use.

Cost-effective river rehabilitation planning: optimizing for morphological benefits at large spatial scales.

River rehabilitation aims to protect biodiversity or restore key ecosystem services but the success rate is often low. This is seldom because of insufficient funding for rehabilitation works but because trade-offs between costs and ecological benefits of management actions are rarely incorporated in the planning, and because monitoring is often inadequate for managers to learn by doing. In this study, we demonstrate a new approach to plan cost-effective river rehabilitation at large scales. The framework is based on the use of cost functions (relationship between costs of rehabilitation and the expected ecological benefit) to optimize the spatial allocation of rehabilitation actions needed to achieve given rehabilitation goals (in our case established by the Swiss water act). To demonstrate the approach with a simple example, we link costs of the three types of management actions that are most commonly used in Switzerland (culvert removal, widening of one riverside buffer and widening of both riversides) to the improvement in riparian zone quality. We then use Marxan, a widely applied conservation planning software, to identify priority areas to implement these rehabilitation measures in two neighbouring Swiss cantons (Aargau, AG and Zürich, ZH). The best rehabilitation plans identified for the two cantons met all the targets (i.e. restoring different types of morphological deficits with different actions) rehabilitating 80,786 m (AG) and 106,036 m (ZH) of the river network at a total cost of 106.1 Million CHF (AG) and 129.3 Million CH (ZH). The best rehabilitation plan for the canton of AG consisted of more and better connected sub-catchments that were generally less expensive, compared to its neighbouring canton. The framework developed in this study can be used to inform river managers how and where best to spend their rehabilitation budget for a given set of actions, ensures the cost-effective achievement of desired rehabilitation outcomes, and helps towards estimating total costs of long-term rehabilitation activities. Rehabilitation plans ready to be implemented may be based on additional aspects to the ones considered here, e.g., specific cost functions for rural and urban areas and/or for large and small rivers, which can simply be added to our approach. Optimizing investments in this way will ultimately increase the likelihood of on-ground success of rehabilitation activities.

The importance of omega-3 polyunsaturated fatty acids as high-quality food in freshwater ecosystems with implications of global change.

Traditionally, trophic ecology research on aquatic ecosystems has focused more on the quantity of dietary energy flow within food webs rather than food quality and its effects on organisms at various trophic levels. Recent studies emphasize that food quality is central to consumer growth and reproduction, and the importance of food quality for aquatic ecosystems has become increasingly well recognized. It is timely to synthesise these findings and identify potential future research directions. We conducted a systematic review of omega-3 polyunsaturated fatty acids (ω3-PUFAs) as a crucial component of high-quality food sources in freshwater ecosystems to evaluate their impact on a variety of consumers, and explore the effects of global change on these high-quality food sources and their transfer to higher trophic consumers within and across ecosystems. In freshwater ecosystems, algae rich in ω3 long-chain PUFAs, such as diatoms, dinoflagellates and cryptophytes, represent important high-quality food sources for consumers, whereas cyanobacteria, green algae, terrestrial vascular plants and macrophytes low in ω3 long-chain PUFAs are low-quality food sources. High-quality ω3-PUFA-containing food sources usually lead to increased growth and reproduction of aquatic consumers, e.g. benthic invertebrates, zooplankton and fish, and also provide ω3 long-chain PUFAs to riparian terrestrial consumers via emergent aquatic insects. Consumers feeding on high-quality ω3-PUFA-containing foods in turn represent high-quality food for their own predators. However, the ω3-PUFA content of food sources is sensitive to global environmental changes. Warming, eutrophication, increased light intensity (e.g. from loss of riparian shading), and pollutants potentially inhibit the synthesis of algal ω3-PUFAs while at the same time promoting the growth of lower-quality foods, such as cyanobacteria and green algae. These factors combined could lead to a significant reduction in the availability of ω3-PUFAs for consumers and constrain their overall fitness. Although the effect of individual environmental factors on high-quality ω3-PUFA-containing food sources has been investigated, multiple environmental factors (e.g. climate change, human activities, pollution) will act in combination and any synergistic effects on aquatic food webs remain unclear. Identifying the sources and fate of ω3-PUFAs within and across ecosystems could represent an important approach to understand the impact of multiple environmental factors on trophic relationships and the implications for populations of freshwater and riparian consumers. Maintaining the availability of high-quality ω3-PUFA-containing food sources may also be key to mitigating freshwater biodiversity loss due to global change.

Increasing water nutrient reduces the availability of high-quality food resources for aquatic consumers and consequently simplifies river food webs.

While eutrophication has led to serious habitat degradation and biotic shifts in freshwater ecosystems, most current studies have focused on changes in community assemblages, with few considering the effect of eutrophication on food webs. We conducted a field study in subtropical headwater streams with a gradient of water nutrient levels to examine the effect of increasing water nutrients on food webs by using the long-chain polyunsaturated fatty acid eicosapentaenoic acid (EPA) as a measure of the nutritional quality of food. Basal food resources (macrophytes, submerged leaf litter, and periphyton), and aquatic consumers (macroinvertebrates and fish) were collected, and their fatty acid (FA) profiles were analyzed. Our results showed that periphyton was the dominant source of EPA for macroinvertebrates and fish, and a high-quality resource for consumers. As water nutrient concentrations increased, nutritional quality of periphyton significantly decreased and, in turn, the correlation between FA profiles of periphyton and macroinvertebrates declined. However, periphyton FA profiles did not account for the variability of fish FA, which may be induced by the increasing proportions of omnivorous fish in eutrophic streams that derived EPA from other sources. Further, the reduced periphyton EPA was associated with decreased trophic links and simplified stream food webs. Our study highlights the importance of high-quality food resources for aquatic food webs as water nutrients increased in stream ecosystems and provides a nutritional perspective to understand the mechanisms how eutrophication affects aquatic ecosystems.

Body size predicts ontogenetic nitrogen stable-isotope (δ15N) variation, but has little relationship with trophic level in ectotherm vertebrate predators.

Large predators have disproportionate effects on their underlying food webs. Thus, appropriately assigning trophic positions has important conservation implications both for the predators themselves and for their prey. Large-bodied predators are often referred to as apex predators, implying that they are many trophic levels above primary producers. However, theoretical considerations predict both higher and lower trophic position with increasing body size. Nitrogen stable isotope values (δ15N) are increasingly replacing stomach contents or behavioral observations to assess trophic position and it is often assumed that ontogenetic dietary shifts result in higher trophic positions. Intraspecific studies based on δ15N values found a positive relationship between size and inferred trophic position. Here, we use datasets of predatory vertebrate ectotherms (crocodilians, turtles, lizards and fishes) to show that, although there are positive intraspecific relationships between size and δ15N values, relationships between stomach-content-based trophic level (TPdiet) and size are undetectable or negative. As there is usually no single value for 15N trophic discrimination factor (TDF) applicable to a predator species or its prey, estimates of trophic position based on δ15N in ectotherm vertebrates with large size ranges, may be inaccurate and biased. We urge a reconsideration of the sole use of δ15N values to assess trophic position and encourage the combined use of isotopes and stomach contents to assess diet and trophic level.

Assessment of the impact of dams on aquatic food webs using stable isotopes: Current progress and future challenges.

Dams have disrupted natural river systems worldwide and although population and community level effects on aquatic biota have been well documented, food web responses remain poorly understood and difficult to characterize. The application of stable isotope analysis (SIA) provides a means to assess the effect of dams on food webs. Here we review the effect of dams on aquatic food webs using SIA, aiming to detect knowledge gaps in the field of dam impacts on aquatic food webs and propose a conceptual framework to help formulate hypotheses about dam impacts on food webs guided by food web theory. Dams can affect aquatic food webs via two pathways: a bottom-up pathway with altered basal food sources and their transfer to consumers through changes in flow, nutrients, temperature and sediment, and a top-down pathway with consumer species composition altered mainly through habitat fragmentation and related physiochemical changes. Taking these mechanisms into consideration, the impact of dams on food web attributes derived from SIA was evaluated. These studies generally apply mixing models to determine how dams alter the dominant carbon sources supporting food webs, use δ15N to examine how dams alter food-chain length, or use Layman metrics of isotope variability to assess niche changes for invertebrate and fish assemblages. Most studies compare the patterns of SIA metrics spatially (e.g. upstream vs reservoir vs downstream of dams; regulated vs unregulated rivers) and temporally (before vs after dam construction), without explicit hypotheses and/or links to theoretical concepts of food webs. We propose several steps to make SIA studies of dam impacts more rigorous and enhance their potential for producing novel insights. Future studies should quantify the shape and strength of the effect of dams on SIA-measured food web response, be conducted at larger temporal and spatial scales (particularly along the river longitudinal continuum and the lateral connected ecosystems (e.g., floodplains)), and consider effects of dams on food web resilience and tipping points.

Identifying the spatial scale of land use that most strongly influences overall river ecosystem health score.

Catchment and riparian degradation has resulted in declining ecosystem health of streams worldwide. With restoration a priority in many regions, there is an increasing interest in the scale at which land use influences stream ecosystem health. Our goal was to use a substantial data set collected as part of a monitoring program (the Southeast Queensland, Australia, Ecological Health Monitoring Program data set, collected at 116 sites over six years) to identify the spatial scale of land use, or the combination of spatial scales, that most strongly influences overall ecosystem health. In addition, we aimed to determine whether the most influential scale differed for different aspects of ecosystem health. We used linear-mixed models and a Bayesian model-averaging approach to generate models for the overall aggregated ecosystem health score and for each of the five component indicators (fish, macroinvertebrates, water quality, nutrients, and ecosystem processes) that make up the score. Dense forest close to the survey site, mid-dense forest in the hydrologically active near-stream areas of the catchment, urbanization in the riparian buffer, and tree cover at the reach scale were all significant in explaining ecosystem health, suggesting an overriding influence of forest cover, particularly close to the stream. Season and antecedent rainfall were also important explanatory variables, with some land-use variables showing significant seasonal interactions. There were also differential influences of land use for each of the component indicators. Our approach is useful given that restoring general ecosystem health is the focus of many stream restoration projects; it allowed us to predict the scale and catchment position of restoration that would result in the greatest improvement of ecosystem health in the regions streams and rivers. The models we generated suggested that good ecosystem health can be maintained in catchments where 80% of hydrologically active areas in close proximity to the stream have mid-dense forest cover and moderate health can be obtained with 60% cover.

Consumer-resource coupling in wet-dry tropical rivers.

1. Despite implications for top-down and bottom-up control and the stability of food webs, understanding the links between consumers and their diets remains difficult, particularly in remote tropical locations where food resources are usually abundant and variable and seasonal hydrology produces alternating patterns of connectivity and isolation. 2. We used a large scale survey of freshwater biota from 67 sites in three catchments (Daly River, Northern Territory; Fitzroy River, Western Australia; and the Mitchell River, Queensland) in Australia's wet-dry tropics and analysed stable isotopes of carbon (δ(13) C) to search for broad patterns in resource use by consumers in conjunction with known and measured indices of connectivity, the duration of floodplain inundation, and dietary choices (i.e. stomach contents of fish). 3. Regression analysis of biofilm δ(13) C against consumer δ(13) C, as an indicator of reliance on local food sources (periphyton and detritus), varied depending on taxa and catchment. 4. The carbon isotope ratios of benthic invertebrates were tightly coupled to those of biofilm in all three catchments, suggesting assimilation of local resources by these largely nonmobile taxa. 5. Stable C isotope ratios of fish, however, were less well-linked to those of biofilm and varied by catchment according to hydrological connectivity; the perennially flowing Daly River with a long duration of floodplain inundation showed the least degree of coupling, the seasonally flowing Fitzroy River with an extremely short flood period showed the strongest coupling, and the Mitchell River was intermediate in connectivity, flood duration and consumer-resource coupling. 6. These findings highlight the high mobility of the fish community in these rivers, and how hydrological connectivity between habitats drives patterns of consumer-resource coupling.

Fish mediate high food web connectivity in the lower reaches of a tropical floodplain river.

High levels of hydrological connectivity during seasonal flooding provide significant opportunities for movements of fish between rivers and their floodplains, estuaries and the sea, possibly mediating food web subsidies among habitats. To determine the degree of utilisation of food sources from different habitats in a tropical river with a short floodplain inundation duration (~2 months), stable isotope ratios in fishes and their available food were measured from three habitats (inundated floodplain, dry season freshwater, coastal marine) in the lower reaches of the Mitchell River, Queensland (Australia). Floodplain food sources constituted the majority of the diet of large-bodied fishes (barramundi Lates calcarifer, catfish Neoarius graeffei) captured on the floodplain in the wet season and for gonadal tissues of a common herbivorous fish (gizzard shad Nematalosa come), the latter suggesting that critical reproductive phases are fuelled by floodplain production. Floodplain food sources also subsidised barramundi from the recreational fishery in adjacent coastal and estuarine areas, and the broader fish community from a freshwater lagoon. These findings highlight the importance of the floodplain in supporting the production of large fishes in spite of the episodic nature and relatively short duration of inundation compared to large river floodplains of humid tropical regions. They also illustrate the high degree of food web connectivity mediated by mobile fish in this system in the absence of human modification, and point to the potential consequences of water resource development that may reduce or eliminate hydrological connectivity between the river and its floodplain.

Polyunsaturated fatty acids in fish tissues more closely resemble algal than terrestrial diet sources.

The River Continuum Concept implies that consumers in headwater streams have greater dietary access to terrestrial basal resources, but recent studies have highlighted the dietary importance of high-quality algae. Algae provide consumers with physiologically important omega-3 (n-3) polyunsaturated fatty acids (PUFA), particularly eicosapentaenoic acid (EPA). However, terrestrial plants and most benthic stream algae lack the long-chain (LC) n-3 PUFA docosahexaenoic acid (DHA, 22:6n-3), which is essential for neural development in fish and other vertebrates. We sampled subalpine streams to investigate how the PUFA composition of neural (brain and eyes), muscle, and liver tissues of freshwater fish is related to their potential diets (macroinvertebrates, epilithon, fresh and conditioned terrestrial leaves). The PUFA composition of consumers was more similar to epilithon than to terrestrial leaves. Storage lipids of eyes most closely resembled dietary PUFA (aquatic invertebrates and algae). However, DHA and arachidonic acid (ARA, 20:4n-6) were not directly available in the diet but abundant in organs. This implies that algal PUFA were selectively retained or were produced internally via enzymatic PUFA conversion by aquatic consumers. This field study demonstrates the nutritional importance of algal PUFA for neural organs in aquatic consumers of headwater regions.