RESUMO
OBJECTIVES: To explore the expression of xeroderma pigmentosum complementation group G ï¼XPGï¼ gene in healthy Han population of different ages and to analysis the relationship between the mRNA and protein expression levels of XPG and age, which may provide a new molecular-biological indicator for forensic age determination. METHODS: Total 150 samples of peripheral blood were collected from healthy Han population of different ages. Total RNA of peripheral blood mononuclear cell ï¼PBMCï¼ were extracted by TRIzol method, and the relative expression of XPG mRNA in PBMC was detected by quantitative real-time PCR, and the protein expression levels of XPG in plasma were detected by ELISA. RESULTS: The mRNA and protein expression levels of XPG in ≤18 years old group were significantly different from 19-45 years old group and ≥46 years old group ï¼P<0.05ï¼, while there was no significant difference between 19-45 years old group and ≥46 years old group ï¼P>0.05ï¼. No significant sex differences were observed in mRNA and protein expression levels of XPG ï¼P>0.05ï¼. CONCLUSIONS: The relative expression level of XPG mRNA in PBMC declines with the increase of age in younger age, while the protein expression level in plasma increases with age, and XPG gene can be used as one of new markers for forensic age estimation.
Assuntos
Fatores Etários , Proteínas de Ligação a DNA/genética , Endonucleases/genética , Proteínas Nucleares/genética , Fatores de Transcrição/genética , Adulto , Povo Asiático , Genética Forense , Humanos , Leucócitos Mononucleares , Pessoa de Meia-Idade , RNA Mensageiro , Reação em Cadeia da Polimerase em Tempo Real , Adulto JovemRESUMO
We early show that glutamate (Glu) mediate hyperoxia-induced newborn rat lung injury through N-methyl-D-aspartate receptor (NMDAR). In this study, we search for evidence of NMDAR expression on newborn rat alveolar macrophages (AMs) and the difference between newborn and adult rat AMs, and the possible effect on nitric oxide (NO) production of AMs by exogenous NMDA. The protein of NMDAR was showed by immunocytochemistry, and the mRNA was examined by RT-PCR and real-time PCR. The results show that: (i) both newborn and adult rat AMs express NMDAR1 and the four NMDAR2 subtypes and newborn rat AMs are higher expression. (ii) NMDA administration increase NO production, inducible nitric oxide synthase (iNOS) activity and iNOS mRNA expression of AMs. (iii) NMDAR activation elevates NO secretion of AMs, which suggests that AM may be one of the key cellular origin of the elevated NO secretion in hyperoxia-induced lung injury.