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1.
Appl Opt ; 63(4): 1039-1047, 2024 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-38437402

RESUMO

A carefully designed daylight collector for a tubular skylight is necessary to serve the occupants' illumination needs under the dynamic trajectory of the sun. This work simulated an improved configuration of a passive daylight collector comprising parabolic and conical reflectors in a modeled room using the lighting software tool TracePro. Results indicated that the lighting performance of the proposed design configuration was significantly enhanced under low altitude sun in comparison with conventional tubular skylights (with revolved parabolic and cylindrical reflectors) [Light. Res. Technol.52, 495 (2020)10.1177/1477153519872794] and hemispherical transparent dome as daylight collectors by more than ∼30%-40% and ∼110%-130%, respectively.

2.
J Food Sci Technol ; 58(12): 4608-4615, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34629525

RESUMO

Loop-mediated isothermal amplification (LAMP) is a diagnostic method for meat speciation with rapid and minimal equipment requirements. In this study, we developed cattle-specific tube-based LAMP assays targeting mitochondrial Cyt b gene sequence, compared with conventional PCR assay for specificity, sensitivity, and validation of the assay was made. The LAMP reaction was carried at 64 °C for 45 min, and results were confirmed by SYBR Green I dye and agarose gel-electrophoresis. The specificity of the assays was cross-tested with DNA of buffalo, goat, sheep, and pork. The amplification was observed with samples from cattle only without cross-reactivity with other meat species. The analytical sensitivity of LAMP and PCR method for cattle DNA detection was 0.0001 ng and 1 ng, respectively. Repeatability of the assay was achieved on samples from known/blind and admixture meat with other than cattle at the relative percentage of 20%, 10%, 5%, and 1%. The study concluded that the developed assay can be easily employed for the rapid identification of tissue of cattle origin in meat and meat products in low resource areas.

3.
J Food Sci Technol ; 57(2): 537-548, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32116363

RESUMO

Natural plant pigment, anthocyanins have the capability to change its color with the change of its structure influenced by changing pH. This feature of anthocyanin has been harnessed to design a meat products quality indicator. In the present experiment anthocyanin rich Jamun fruit (Syzgium cumini) skin extract was used to develop quality indicator by immobilizing on filter paper strips with the purpose of application in chicken patties packets stored at refrigeration temperature (4 ± 1 °C). The indicator changed its color from violet to yellow due to changed pH in it when it was attached inside packet of chicken patties during storage, due to reaction with volatile basic compounds generated from meat. During storage for 21 days, various changes in quality attributes of chicken patties viz., pH, Total volatile basic nitrogen (TVBN), ammonia level, color value, sensory attributes and microbial evaluation were estimated. The pH decreased (P < 0.5) from 6.22 to 6.04. TVBN and ammonia and level increased significantly (P < 0.5) throughout storage. Redness, yellowness, hue and chroma value gradually changed during storage. Sensory scores also decreased significantly (P < 0.5). Microbial count also increased (P < 0.5) during this time. The experiment showed that, during storage, the color changing pattern of quality indicator was well correlated with the changes in quality attributes of chicken meat patties. Therefore, it is expected that the developed quality indicator can provide a convenient, non destructive, visual mean to monitor the meat products quality during refrigerated storage.

4.
Lupus ; 28(6): 731-739, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31023131

RESUMO

BACKGROUND: Acute pancreatitis is an uncommon complication that occurs in 0.85% to 4% of patients with systemic lupus erythematosus (SLE). In some patients, it occurs within days to weeks of starting medium-to-high dose corticosteroids. The authors have used the term 'corticosteroid-associated lupus pancreatitis' for these patients, and they report a case series and perform a systematic review of previously published reports. METHODS: For the purpose of this study, corticosteroid-associated lupus pancreatitis was defined as occurrence of acute pancreatitis in patients with SLE (fulfilling the 1997 ACR), within 3 weeks of starting therapy with medium-to-high dose corticosteroids - either newly initiated or escalated from a lower dose. All patients with SLE admitted in the last 2.5 years in a North Indian university hospital were reviewed, and those with pancreatitis who fulfilled the above criteria were included in the case series. For the systematic review, a PUBMED search using the keywords 'lupus' and 'pancreatitis' was performed, and reports in English were reviewed for an association with corticosteroids. RESULTS: Among 420 admissions of SLE patients, six patients (1.4%) fulfilled criteria for corticosteroid-associated lupus pancreatitis. All were female, with mean age and disease duration of 19.7 ± 3.3 and 3.8 ± 2.5 years respectively. All had active disease and developed acute pancreatitis within 48-72 hours of newly initiating medium-to-high dose corticosteroids (in three patients) or escalating them to medium-high dose (in three patients). After the development of pancreatitis, corticosteroids were continued in all except one patient. In addition, two patients received pulse methylprednisolone, two received pulse cyclophosphamide and one was started on azathioprine. Three patients died during hospitalization, all with severe pancreatitis. On systematic review, among 451 cases of lupus pancreatitis reported, 23 (5%) fulfilled criteria for 'corticosteroid-associated lupus pancreatitis'. A majority of them had pancreatitis within 3 days of starting treatment with medium-to-high dose corticosteroids. The mortality in these patients was 37.5%. CONCLUSION: In a small but substantial proportion of patients with lupus who develop pancreatitis, it occurs within days to weeks of starting medium-to-high dose corticosteroids. Many of these patients continue to receive corticosteroids, and some receive more aggressive immunosuppression. However, they have significant mortality, and further studies are required to identify appropriate treatment in this subgroup of patients.


Assuntos
Corticosteroides/uso terapêutico , Lúpus Eritematoso Sistêmico/complicações , Lúpus Eritematoso Sistêmico/tratamento farmacológico , Pancreatite/induzido quimicamente , Doença Aguda , Adolescente , Corticosteroides/efeitos adversos , Azatioprina/uso terapêutico , Ciclofosfamida/uso terapêutico , Relação Dose-Resposta a Droga , Feminino , Humanos , Pancreatite/mortalidade , Pancreatite/patologia , Índice de Gravidade de Doença , Adulto Jovem
5.
J Appl Microbiol ; 119(4): 991-8, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-26218659

RESUMO

AIMS: To report fungal and aflatoxin contamination in stored tobacco leaves and the potential of Foeniculum vulgare (fennel) seed essential oil (EO) as a plant-based preservative in protection of tobacco during storage. METHODS AND RESULTS: Mycological analysis of tobacco samples was done by surface sterilization and serial dilution tests. The Aspergillus flavus isolates were screened for their toxigenicity. Both in vivo and in vitro tests were done to evaluate antifungal and antiaflatoxigenic efficacy of chemically characterized EO. The mycoflora analysis revealed 108 fungal colonies belonging to five genera and nine species. All A. flavus isolates were found aflatoxigenic during screening. Gas chromatography and mass spectrometry analysis of EO identified 19 components (99·66%); estragole being the major component (47·49%). The EO showed broad fungitoxicity at 1·25 µl ml(-1) and 100% inhibition to AFB1 production as well as ergosterol synthesis at 1·0 µl ml(-1) concentration. EO showed 100% protection of stored tobacco samples from aflatoxin B1 contamination. CONCLUSIONS: The fennel EO can thus be formulated as a plant-based preservative for food items. SIGNIFICANCE AND IMPACT OF THE STUDY: The present investigation comprises the first report on antiaflatoxin efficacy of fennel oil and its potency in the protection of tobacco leaves from fungal and aflatoxin contamination during storage.


Assuntos
Aflatoxina B1/biossíntese , Antifúngicos/farmacologia , Aspergillus flavus/efeitos dos fármacos , Foeniculum/química , Nicotiana/microbiologia , Óleos Voláteis/farmacologia , Doenças das Plantas/prevenção & controle , Extratos Vegetais/farmacologia , Aflatoxina B1/análise , Aspergillus flavus/genética , Aspergillus flavus/metabolismo , Óleos Voláteis/química , Doenças das Plantas/microbiologia , Extratos Vegetais/química , Folhas de Planta/química , Folhas de Planta/microbiologia , Sementes/química , Nicotiana/química
6.
Artigo em Inglês | MEDLINE | ID: mdl-24427063

RESUMO

In the title compound, C30H28N4O, the central pyrrolidine ring adopts an envelope conformation with the CH2 C atom as the flap. The quinoxaline and indene ring systems are planar, with r.m.s. deviations of 0.0165 and 0.0181 Å, respectively. The pyrrolidine ring mean plane forms dihedral angles of 88.84 (1) and 86.14 (1)° with the quinoxaline and indene ring systems, respectively. A weak intra-molecular C-H⋯N inter-action is observed. In the crystal, C-H⋯O inter-actions lead to helical supra-molecular chains along the b axis having a C(9) motif.

7.
Asian-Australas J Anim Sci ; 25(5): 733-7, 2012 May.
Artigo em Inglês | MEDLINE | ID: mdl-25049620

RESUMO

Determination of sex origin of cattle meat by fast and reliable molecular methods is an important measure to ensure correct allocation of export refunds particularly in European countries and also female cattle (cow) slaughter is legally banned in India because of religious beliefs. Based on the DEAD box protein gene located on the X and Y chromosomes, 2 pair of primers were designed and the system of PCR was optimized. Upon PCR amplification, male tissue showed 2 bands, while female tissue resulted in only one band. The accuracy and specificity of the primers was assessed using DNA template extracted from cattle meat of known sex. The protocol was subjected to a blind test and showed 100% concordance, proving its accuracy and reliability.

8.
J Food Sci Technol ; 49(2): 246-51, 2012 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-23572849

RESUMO

Study was performed to determine the effect of post-mortem handling conditions on the physico-chemical and sensory attributes of spent hen meat curry. Breast cuts of spent hens were subjected to different postmortem handling conditions before cooking viz; made into small cuts and cooked within 1-2 h of slaughter (condition 1), made into small cuts and cooked after 4-5 h of slaughter (condition 2), made into small cuts immediately after slaughter, stored at 4 ± 1 °C for 12 h and then cooked (condition 3), stored at 4 ± 1 °C for 12 h, made into small cuts and cooked (condition 4). The pH of meat just before cooking due to different stages of rigor development under various conditions differed accordingly. Observed differences in temperature of meat just before cooking were because of different postmortem handling condition variations viz:1,2,3,&4. The associated post mortem changes under different postmortem handling conditions before cooking led to significant variation in Water holding capacity, Water Soluble Protein, Salt Soluble Protein, cooking yield, moisture percentage before cooking and after cooking and also WB shear force value. In general, sensory scores were higher for conditions 4 and 1 as compared to conditions 2 and 3. Results revealed that quality attributes of spent hen meat curry can be improved by following proper post-slaughter handling and processing conditions. To get meat curry of good sensory quality, meat should be cooked preferably within 1-2 h of slaughter or after 10-12 h of storage of intact carcass at 4 ± 1 °C. Cuts should be made just before cooking but cooking after 4-5 h of slaughter should be avoided.

9.
Biomed Res Int ; 2022: 2003184, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35958813

RESUMO

Prenatal heart disease, generally known as cardiac problems (CHDs), is a group of ailments that damage the heartbeat and has recently now become top deaths worldwide. It connects a plethora of cardiovascular diseases risks to the urgent in need of accurate, trustworthy, and effective approaches for early recognition. Data preprocessing is a common method for evaluating big quantities of information in the medical business. To help clinicians forecast heart problems, investigators utilize a range of data mining algorithms to examine enormous volumes of intricate medical information. The system is predicated on classification models such as NB, KNN, DT, and RF algorithms, so it includes a variety of cardiac disease-related variables. It takes do with an entire dataset from the medical research database of patients with heart disease. The set has 300 instances and 75 attributes. Considering their relevance in establishing the usefulness of alternate approaches, only 15 of the 75 criteria are examined. The purpose of this research is to predict whether or not a person will develop cardiovascular disease. According to the statistics, naïve Bayes classifier has the highest overall accuracy.


Assuntos
Mineração de Dados , Cardiopatias , Algoritmos , Teorema de Bayes , Mineração de Dados/métodos , Cardiopatias/diagnóstico por imagem , Humanos , Processamento de Imagem Assistida por Computador
10.
N Z Dent J ; 106(3): 113-4, 2010 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-20882741

RESUMO

AIM: The objective of this study was to describe the demographic characteristics of a case series of patients with a clinical and histopathological diagnosis of oral lichen planus who presented to the Oral Health Unit (Auckland District Health Board) between the years 1999 and 2006 (an 8-year period). METHOD: The records of patients who had a clinical and histopathological diagnosis of oral lichen planus were audited. Demographic (age, gender, ethnicity), clinical and histopathological data were collated. Patients whose ethnicity was not recorded were noted but not included in this part of the analysis. RESULTS: Between 1999 and 2006, 267 patients (82 males, 185 females; age range 21 to 93 years) had a clinical and histopathological diagnosis of oral lichen planus. Ethnicity data were available for 207 of those, of whom 108 (52.2%) were European; 65 (31.4%) of Indian subcontinent origin (India, Pakistan, Sri Lanka); 20 (9.7%) were Asian; 14 (6.8%) were Pacific Islanders; 50 (24.2%) were 'Other'; and 10 (4.8%) were 'Unknown'. None was Maori. CONCLUSION: The age and gender distribution in this case series were similar to that observed in a comparable UK study. The major findings were in the ethnic distribution of oral lichen planus in the sample, with patients of Indian subcontinent origin being over-represented, and Maori being under-represented.


Assuntos
Líquen Plano Bucal/epidemiologia , Adulto , Fatores Etários , Idoso , Idoso de 80 Anos ou mais , Povo Asiático/estatística & dados numéricos , Auditoria Odontológica , Etnicidade/estatística & dados numéricos , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Havaiano Nativo ou Outro Ilhéu do Pacífico/estatística & dados numéricos , Nova Zelândia/epidemiologia , Fatores Sexuais , População Branca/estatística & dados numéricos , Adulto Jovem
11.
Trop Anim Health Prod ; 41(8): 1695-700, 2009 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-19455400

RESUMO

Epidemiological studies were conducted on Fasciola giagntica infection in different geo-climatic zones (hills, tarai and plains) in six states of north India during January 2001 to December 2004. Prevalence rates of 10.79, 13.90, 2.78 and 2.35 percent were recorded in cattle, buffaloes, sheep and goats, respectively. Animals in the tarai had the highest prevalence of fasciolosis followed by those in the hills and plains, respectively. The prevalence of infection in cattle and buffaloes was highest during the winters (11.84% cattle, 15.57% buffaloes) followed by summers and rains, respectively. However, the seasonal trends in sheep and goats were the reverse, with the peak prevalence during the rains (4.60% sheep, 2.71% goats). Abattoir studies revealed a higher prevalence in buffaloes (31.14%) than in sheep and goats. Screening of Lymnaea auricularia snails revealed that 5.48% of the snails harboured larval stages of F. gigantica. Also, the snails in the tarai had a higher prevalence (7.28%) of infection compared to those in the plains (1.57%). The results of the present study may help to devise an effective control strategy against fasciolosis in north India.


Assuntos
Fasciolíase/veterinária , Matadouros , Animais , Meio Ambiente , Fasciolíase/epidemiologia , Índia/epidemiologia , Prevalência , Ruminantes , Estações do Ano
12.
Meat Sci ; 80(2): 418-22, 2008 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-22063348

RESUMO

To assess microbiological quality of buffalo meat trimmings (TT=114) and silver sides (SS=41), samples were collected from four different Indian meat packing plants. The aim of this study was: (i) to evaluate standard plate count (SPC), psychrotrophic count (PTC), Enterococcus feacalis count (EFC), Staphylococcus aureus count (SAC) and Escherichia coli count (ECC) and the presence of Salmonella spp. and Listeria monocytogenes; and (ii) also to determine vero toxic E. coli (VTEC) by polymerase chain reaction (PCR). TT samples had significantly higher (P<0.001) SPC, PTC, EFC, and SAC than SS, while across the meat types there was no difference (P>0.05) in ECC. E. coli was recovered from 32.4% TT and 19.5% SS samples. The prevalence rate of Salmonella spp. and L. monocytogenes in TT was 1.75% and 0.87%, respectively. But no SS sample was found to be positive for any of these two pathogens. VTEC was found in 2.58% of all the tested samples. This finding suggests that TT contain higher microbes but only small numbers of pathogens of latent zoonotic importance. The present study confirmed the importance of maintaining good process hygiene at meat plants for microbiological status of buffalo meat.

13.
Indian J Physiol Pharmacol ; 52(3): 307-10, 2008.
Artigo em Inglês | MEDLINE | ID: mdl-19552065

RESUMO

Obesity by itself is not an immediate lethal disease, however, it is a significant risk factor associated with a range of serious non-communicable diseases. Our aim is to find out the prevalence of metabolic syndrome in interns coming from higher socioeconomic class. Sixty one interns participated in the study. BMI, WHR and blood chemistry were performed. EGIR criteria were considered to identify the metabolic syndrome. According to EGIR criteria, insulin resistance was found in 24.5% cases and metabolic syndrome exists in 14.7% of overweight/obese interns. Insulin resistance as per HOMA-IR was found in 6% of those having metabolic syndrome. High diastolic blood pressure, low HDLc are prone to develop metabolic syndrome by six times where as an increase in WHR (central obesity) particularly in male are eleven times more prone to develop metabolic syndrome.


Assuntos
Síndrome Metabólica/epidemiologia , Obesidade/complicações , Adulto , Estudos de Coortes , Feminino , Humanos , Índia/epidemiologia , Resistência à Insulina , Masculino , Síndrome Metabólica/complicações , Síndrome Metabólica/diagnóstico , Sobrepeso/complicações , Prevalência , Classe Social
14.
Vet Res Commun ; 31(6): 749-55, 2007 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-17237985

RESUMO

We report the results of investigations that were conducted in a sheep flock in Uttaranchal, India where repeated failure of anthelmintic medication was noted. The study revealed that Haemonchus contortus in sheep had developed resistance to benzimidazoles (fenbendazole, mebendazole and albendazole), imidazothiazole (levamisole) and salicylanide (rafoxanide), while it was fully susceptible to avermectins (ivermectin). Further, the suppression of nematode egg output in faeces of sheep naturally infected with multiple anthelmintic-resistant H. contortus following treatment with ivermectin tablet (0.4 mg/kg body weight (bw), orally), ivermectin injection (1% w/v, 0.2 mg/kg bw, subcutaneously) and ivermectin pour-on (0.5 w/v, 0.5 mg/kg bw) was also studied over a period of 10 weeks post treatment. It was noted that ivermectin tablet after initial clearance of infection (faecal egg count reduction 100%), could not prevent establishment of new patent natural infection for even a single day, while ivermectin pour-on and injection prevented the establishment of new infection for 7 and 14 days post treatment, respectively. Maximum protection period (duration for which mean faecal egg count of sheep reaches 500 eggs per gram of faeces or more) of 68 days was recorded in sheep treated with injectable ivermectin, followed by pour-on (60 days) and oral (53 days) preparations.


Assuntos
Anti-Helmínticos/administração & dosagem , Resistência a Múltiplos Medicamentos , Gastroenteropatias/veterinária , Hemoncose/veterinária , Haemonchus/crescimento & desenvolvimento , Ivermectina/administração & dosagem , Doenças dos Ovinos/tratamento farmacológico , Doenças dos Ovinos/parasitologia , Administração Oral , Administração Tópica , Albendazol/farmacologia , Animais , Fezes/parasitologia , Feminino , Fenbendazol/farmacologia , Gastroenteropatias/tratamento farmacológico , Gastroenteropatias/parasitologia , Hemoncose/tratamento farmacológico , Hemoncose/parasitologia , Injeções Subcutâneas/veterinária , Masculino , Mebendazol/farmacologia , Contagem de Ovos de Parasitas/veterinária , Ovinos
15.
Circulation ; 104(12 Suppl 1): I265-9, 2001 Sep 18.
Artigo em Inglês | MEDLINE | ID: mdl-11568067

RESUMO

BACKGROUND: Extracorporeal circulation induces a systemic inflammatory response, which may adversely affect organ function. One manifestation of this response is increased fibrinolysis. Antifibrinolytic drugs such as aprotinin and epsilon-aminocaproic acid have been effective in reducing fibrinolysis and blood loss after extracorporeal circulation; however, the effects of antifibrinolytic drugs on proinflammatory and anti-inflammatory mediators are not known. This study examined the effects of aprotinin and epsilon-aminocaproic acid on plasma levels of proinflammatory [interleukin-6 (IL-6)] and anti-inflammatory [interleukin-10 (IL-10)] cytokines during and after extracorporeal circulation. METHODS AND RESULTS: Seventy-two patients undergoing coronary artery bypass grafting with extracorporeal circulation were randomly assigned in a double-blind study to receive high-dose aprotinin, epsilon-aminocaproic acid, or saline placebo. Plasma levels of IL-6 and IL-10 were measured at 5 time points before, during, and after extracorporeal circulation. In all 3 groups, both IL-6 and IL-10 rose significantly after institution of extracorporeal circulation and remained elevated through the first postoperative day. Compared with saline, aprotinin significantly reduced IL-10 (P=0.02) and peak IL-6 (P=0.02) after extracorporeal circulation. In contrast, none of the reductions in IL-6 and IL-10 by epsilon-aminocaproic acid achieved statistical significance. Both aprotinin and epsilon-aminocaproic acid decreased blood loss compared with saline, but there was no significant difference in the number of patients receiving blood products among the treatment groups. CONCLUSIONS: These data suggest that aprotinin and epsilon-aminocaproic acid differ in their effects on the inflammatory response to extracorporeal circulation. Aprotinin but not epsilon-aminocaproic acid appears to attenuate the rise in the proinflammatory and anti-inflammatory cytokines IL-6 and IL-10. Further studies will be required to determine if these cytokine alterations translate to changes in clinical outcomes.


Assuntos
Ácido Aminocaproico/administração & dosagem , Antifibrinolíticos/administração & dosagem , Aprotinina/administração & dosagem , Interleucina-10/sangue , Inibidores de Serina Proteinase/administração & dosagem , Síndrome de Resposta Inflamatória Sistêmica/prevenção & controle , Ponte de Artéria Coronária , Método Duplo-Cego , Circulação Extracorpórea/efeitos adversos , Fibrinólise/efeitos dos fármacos , Humanos , Interleucina-6/sangue , Pessoa de Meia-Idade , Período Pós-Operatório , Síndrome de Resposta Inflamatória Sistêmica/sangue , Síndrome de Resposta Inflamatória Sistêmica/etiologia , Resultado do Tratamento
16.
Hum Exp Toxicol ; 34(7): 780-3, 2015 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-25352650

RESUMO

This study evaluated the protective potential of curcumin on the possible side effects of bortezomib (Bt) therapy on normal cells in mice. The mice were segregated into three groups (n = 10) that included normal control, Bt-treated, and Bt + curcumin-treated groups. The Bt treatment resulted in significant decrease in the enzyme activity of erythrocyte δ-aminolevulinic acid dehydratase (ALAD). Also a significant decrease in the hemoglobin (Hb) was also noticed. On the other hand, curcumin co-treatment improvised enzyme activity of erythrocyte ALAD as well as Hb values. The study, therefore, concludes that curcumin co-treatment with Bt has a potential to take care of possible side effects of Bt therapy on normal cells.


Assuntos
Antineoplásicos/efeitos adversos , Bortezomib/efeitos adversos , Curcumina/farmacologia , Substâncias Protetoras/farmacologia , Animais , Antineoplásicos/uso terapêutico , Bortezomib/uso terapêutico , Eritrócitos/efeitos dos fármacos , Eritrócitos/metabolismo , Hemoglobinas/análise , Masculino , Camundongos , Sintase do Porfobilinogênio/sangue
17.
Endocrinology ; 131(1): 166-72, 1992 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-1319317

RESUMO

Histamine-secreting enterochromaffin-like (ECL) cells of the gastric fundus of the Mastomys can develop into solid ECL cell tumors, either spontaneously or after induction by acid inhibition. We used this tumor tissue to perform in vitro receptor autoradiography for somatostatin (SS), gastrin, and substance-P, using, respectively, [125I]Tyr3-octreotide, [125I]gastrin-17, and [125I]Bolton-Hunter-labeled substance-P as radioligands. A high density of SS receptors was found in the nontumor fundic mucosa, where gastrin receptors were only barely detectable. However, in the group of spontaneously developing ECL cell tumors, a high density of SS and gastrin receptors was observed, homogeneously distributed in the tumor tissue. In addition, the loxtidine-induced ECL cell tumors expressed a high density of SS and gastrin receptors. The receptors were specific for the respective peptide and of high affinity, with a dissociation constant (Kd) of 0.90 nM for SS receptor and 0.87 nM for gastrin receptors. No substance-P receptors were detected on the ECL cell tumors, although they were present in the muscle layers of the Mastomys gastric fundus. These results demonstrate that ECL-derived tumors express receptors for both SS and gastrin. This observation is consistent with the proposal that there is substantial regulation of the histamine-producing ECL cell by SS and gastrin. The presence of gastrin receptors is compatible with a role for gastrin as a trophic factor in ECL cell hyperplasia and neoplasia. The expression of SS receptors may be of diagnostic and therapeutic relevance in the regulation of ECL function and neoplastic transformation.


Assuntos
Células Enterocromafins/metabolismo , Muridae , Receptores da Colecistocinina/metabolismo , Receptores de Neurotransmissores/metabolismo , Neoplasias Gástricas/veterinária , Animais , Autorradiografia , Mucosa Gástrica/metabolismo , Receptores da Neurocinina-1 , Receptores de Somatostatina , Neoplasias Gástricas/metabolismo
18.
Int J Cardiol ; 31(2): 245-7, 1991 May.
Artigo em Inglês | MEDLINE | ID: mdl-1869335

RESUMO

A 24-year-old male presented with exertional dyspnoea of one year's duration. Echocardiography revealed an unruptured aneurysm of the left sinus of Valsalva distorting the mitral valvar apparatus and producing severe regurgitation in the absence of myocardial ischemia or infarction. We propose an alternate mechanism for the mitral regurgitation.


Assuntos
Aneurisma Aórtico/complicações , Insuficiência da Valva Mitral/etiologia , Seio Aórtico , Adulto , Aneurisma Aórtico/congênito , Aneurisma Aórtico/diagnóstico , Dispneia/diagnóstico , Dispneia/etiologia , Ecocardiografia , Humanos , Masculino
19.
Int J Cardiol ; 41(3): 191-9, 1993 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-8288408

RESUMO

We conducted a randomised controlled trial to study the effects of metoprolol and diltiazem on the total ischaemic burden--sum of symptomatic and silent myocardial ischaemia, in 146 patients with stable angina pectoris. One-hundred thirty-four completed the study protocol. Sixty-eight patients received metoprolol (100 mg twice daily, n = 52, 50 mg twice daily, n = 16) while 66 received diltiazem (90 mg three times daily, n = 50, 60 mg three times daily, n = 16). The drugs were given for 4 weeks. The primary outcome variables were frequency and duration of total ischaemic burden, silent and symptomatic myocardial ischaemia. These were measured on 48 h of Holter monitoring. The reductions in duration and frequency of total ischaemic burden by metoprolol, 76% and 40%, respectively, were significantly higher than by diltiazem, 43% and 24%, respectively (P < 0.01 and P < 0.02). The frequency and duration of silent myocardial ischaemia, which constituted more than 80% of the total ischaemic burden in the two groups showed similar results. However, the reduction in frequency of symptomatic myocardial ischaemia only was significantly greater by metoprolol (63% than diltiazem (24%) as the difference in reduction of duration of symptomatic ischaemia was insignificant (85% vs. 75%; P > 0.05). Whether a greater reduction of total ischaemic burden by metoprolol as compared to diltiazem has any implications for prognosis in patients with chronic stable angina remains to be established.


Assuntos
Diltiazem/uso terapêutico , Metoprolol/uso terapêutico , Isquemia Miocárdica/prevenção & controle , Angina Pectoris/tratamento farmacológico , Eletrocardiografia Ambulatorial , Teste de Esforço , Feminino , Frequência Cardíaca/efeitos dos fármacos , Humanos , Masculino , Metoprolol/efeitos adversos , Pessoa de Meia-Idade
20.
Am Surg ; 65(5): 453-5, 1999 May.
Artigo em Inglês | MEDLINE | ID: mdl-10231216

RESUMO

Appendicitis is a common surgical disease that often presents with vague, inconclusive symptoms. Despite the development of technologically advanced diagnostic modalities, perforation has usually occurred before the surgeon sees the patient. Morbidity, length of stay, and hospital costs associated with appendiceal perforation have not changed markedly in the past 50 years. To evaluate prognostic markers for severe appendicitis, we reviewed 18 years' worth of records of patients with appendicitis who were admitted to St. Elizabeth's Medical Center of Boston and found that the combination of history and physical examination has withstood the test of time. We found that advancing age, duration of symptoms for 36 or more hours, white blood cell count, shift left in white blood cell count, and fever were significantly related to severe appendicitis. An abnormal plain film was a marker if fever coexisted.


Assuntos
Apendicite/diagnóstico , Adolescente , Adulto , Idoso , Apendicite/complicações , Apendicite/patologia , Boston , Feminino , Gangrena , Humanos , Perfuração Intestinal/etiologia , Masculino , Prontuários Médicos , Pessoa de Meia-Idade , Estudos Retrospectivos , Fatores de Risco , Índice de Gravidade de Doença
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