Comparison with others influences encoding and recognition of their faces: Behavioural and ERP evidence.

In daily life, faces are often memorized within contexts involving interpersonal interactions. However, little is known about whether interpersonal interaction-related contexts influence face memory. The present study aimed to understand this question by investigating how social comparison-related context affects face encoding and recognition. To address this issue, 40 participants were informed that they and another player each played a monetary game and were then presented with both of their outcomes (either monetary gain or loss). Subsequently, participants were shown the face of the player whom they were just paired with. After all the faces had been encoded, participants were asked to perform a sudden old/new recognition task involving these faces. The results showed that, during the encoding phase, another player's monetary gain, compared to loss, resulted in more negative responses in the N170 and early posterior negativity (EPN)/N250 to relevant players' faces when participants encountered monetary loss and a smaller late positive potential (LPP) response irrespective of self-related outcomes. In the subsequent recognition phase, preceding another player's monetary gain as compared to loss led to better recognition performance and stronger EPN/N250 and LPP responses to the faces of relevant players when participants had lost some amount of money. These findings suggest that the social comparison-related context, particularly self-disadvantageous outcomes in the context, influences the memory of comparators' faces.

Competition influences outcome processing involving social comparison: An ERP study.

In a complicated social context, outcome evaluation involves not only oneself but also others in relation to the self (i.e., social comparison). Previous event-related potential (ERP) studies have investigated the processing of social comparison-related outcomes when one's interests are independent of the interests of others (i.e., noncompetition circumstances). However, it is unclear how social comparison-related outcomes are processed in the brain when there are conflicts of interest between oneself and others (i.e., competition circumstances). To address this issue, participants in the current study were asked to perform an attentional task with several peers and were subsequently presented with self-related outcomes (i.e., the performance difference between the current trial and several preceding trials) and social comparison-related outcomes (i.e., the performance difference between oneself and their peer). Importantly, rewards and punishments were based on social comparison-related outcomes in the competition condition and on self-related outcomes in the noncompetition condition. ERP results revealed that in the competition condition, positive outcomes involving social comparison elicited a greater P300 response than negative outcomes, whereas this effect was not observed in the noncompetition condition. Additionally, there was generally a larger late positive potential (LPP) response to negative outcomes involving social comparison than to positive outcomes only when one obtained a self-related positive outcome in the competition condition. These findings suggest that competition might strengthen outcome processing involving social comparison at late time ranges relying on self-related outcomes to some extent.

The influence of Tai Chi exercise on the subjective well-being in the aged: the mediating role of physical fitness and cognitive function.

This study investigated the effects of Tai Chi exercise on subjective well-being in the aged. The participants were randomly assigned to an experimental group or a control group. The experimental group received 12-week Tai Chi exercise while the control group maintain their original living habits. The participants' subjective well-being, physical fitness, self-control, and executive function were measured at baseline and after 12 weeks of Tai Chi exercise. Results: (1) Tai Chi exercise can positively affect the subjective well-being of the aged (F(1,78) = 37.699, p < 0.001); (2) Tai Chi exercise could affect the subjective well-being in the aged through the independent intermediary of physical fitness (95% CI=[0.115, 0.485]) and self-control (95% CI=[0.109, 0.433]); (3) Tai Chi exercise could indirectly affect the subjective well-being in the aged through the chain mediation of executive function and self-control (95% CI=[0.009, 0.104]). This study provides valuable insights into the potential benefits of Tai Chi exercise for subjective well-being in the aged.

Behavioral and ERP effects of encoded facial expressions on facial identity recognition depend on recognized facial expressions.

Behavioral and event-related potential (ERP) studies have shown that the expressions of a face displayed in the encoding phase (encoded facial expressions) influences identity recognition of this face in a later recognition phase. As facial expressions displayed in the recognition phase (recognized facial expressions) might also influence facial identity recognition, the current study investigated whether the effect of encoded facial expressions on facial identity recognition changed depending on recognized facial expressions. Therefore, participants were asked to learn facial identities displaying angry, happy or neutral expressions during the encoding phase. In the subsequent recognition phase, participants were presented with the learned identities and several novel identities and asked to judge whether the prompted identities had been learned. Each identity displayed angry, happy and neutral expressions. The results showed that d' scores were smaller for happy-recognized identities (i.e., learned identities displaying happy expressions during the recognition phase) in the angry-encoded (i.e., learned identities that had displayed angry expressions during the preceding encoding phase) condition than in the neutral-encoded condition and for neutral-recognized identities in the angry-encoded condition than in the happy-encoded condition. ERP results showed that angry-encoded identities elicited less negative responses in the P200 and early posterior negativity (EPN)/N250 components and less positive responses in the late positive potential (LPP) component than happy-encoded identities. For neutral-recognized identities, LPP responses were reduced in the angry-encoded condition compared with those in the neutral-encoded condition. These findings might contribute to understanding the influence of previous and current facial expressions on facial identity recognition.

Thoracic radiotherapy and concurrent almonertinib for unresectable stage III EGFR-mutated non-small-cell lung cancer: a phase 2 study.

BACKGROUND: Concurrent chemo-radiotherapy remains the standard treatment in unresectable stage III non-small-cell lung cancer (NSCLC) patients. Several studies have shown a potential value of concurrent epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI) with thoracic radiotherapy in EGFR-mutated population, but a high risk of radiation pneumonitis raised a major concern. This study intends to explore the safety and efficacy of concurrent almonertinib, a new third-generation EGFR-TKI, with radiotherapy in locally advanced EGFR-mutated NSCLC patients. METHODS: Locally advanced NSCLC patients harboring sensitive EGFR mutation will be included in this study. A radiotherapy plan will be made for each patient before treatment, and the lung V20 will be calculated. Patients with lung V20 ≥ 28% were enrolled in induction group (arm A), which almonertinib was given for 2 months followed by concurrent radiotherapy; patients with lung V20 < 28% were enrolled in concurrent group (arm B), which almonertinib was given concurrent with thoracic radiotherapy. The primary endpoint is the incidence of grade ≥ 3 radiation pneumonitis within 6 months post-radiotherapy, and the secondary endpoints are local control rate, progression-free survival, and overall survival. DISCUSSION: The safety and efficacy of third-generation EGFR-TKI concurrent with thoracic radiotherapy in locally advanced EGFR-mutated NSCLC is still unknown. We propose to conduct this phase 2 study evaluating the safety especially the radiation pneumonitis within 6 months post-radiotherapy. This trial protocol has been approved by the Ethics committee of Hangzhou cancer hospital. The ethics number is HZCH-2020-030. TRIAL REGISTRATION: clinicaltrials.gov, NCT04636593 . Registered 19 November 2020 - Retrospectively registered.

Inhibition of histamine receptor H3 suppresses the growth and metastasis of human non-small cell lung cancer cells via inhibiting PI3K/Akt/mTOR and MEK/ERK signaling pathways and blocking EMT.

Recent evidence shows that the expression levels of histamine receptor H3 (Hrh3) are upregulated in several types of cancer. However, the role of Hrh3 in non-small cell lung cancer (NSCLC) has not been elucidated. In the present study, we showed that the expression levels of Hrh3 were significantly increased in NSCLC samples, and high levels of Hrh3 were associated with poor overall survival (OS) in NSCLC patients. In five human NSCLC cell lines tested, Hrh3 was significantly upregulated. In NSCLC cell lines H1975, H460, and A549, Hrh3 antagonist ciproxifan (CPX, 10-80 µM) exerted moderate and concentration-dependent inhibition on the cell growth and induced apoptosis, whereas its agonist RAMH (80 µM) reversed these effects. Furthermore, inhibition of Hrh3 by CPX or siRNA retarded the migration and invasion of NSCLC cells through inhibiting epithelial-mesenchymal transition (EMT) progression via reducing the phosphorylation of PI3K/Akt/mTOR and MEK/ERK signaling pathways. In nude mice bearing H1975 cell xenograft or A549 cell xenograft, administration of CPX (3 mg/kg every other day, intraperitoneal) significantly inhibited the tumor growth with increased E-cadherin and ZO-1 expression and decreased Fibronectin expression in tumor tissue. In conclusion, this study reveals that Hrh3 plays an important role in the growth and metastasis of NSCLC; it might be a potential therapeutic target against the lung cancer.

Effects of experienced regret on risky decision making are dependent on risky degree.

We investigated whether experienced regret influences risky decision making in future dissimilar situations and whether this effect is affected by risky degree. Therefore, participants (N = 39 and 54 in Experiment 1 and 2, respectively) were asked to select one of the two options. In the experienced regret condition, the selected option was worse than the unselected option; in the control condition, the information about the unselected option was unknown to the participants. Subsequently, participants were required to either keep the selected option or to gamble. Additionally, Experiment 2 varied in risky degree of the gamble from low to high. Results showed that experienced regret enhanced possibilities of gamble under low risk (Experiment 1 and 2). Under high risk, however, the effect of experienced regret was reversed (Experiment 2). The findings might suggest that experienced regret affects risky decision making in future dissimilar situations dependently on risky degree.

Chalcomoracin inhibits cell proliferation and increases sensitivity to radiotherapy in human non-small cell lung cancer cells via inducing endoplasmic reticulum stress-mediated paraptosis.

Chalcomoracin (CMR) is a kind of Diels-Alder adduct extracted from the mulberry leaves. Recent studies showed that CMR has a broad spectrum of anticancer activities and induces paraptosis in breast cancer and prostate cancer cells. In this study, we investigated the effects of CMR against human non-small cell lung cancer cells and the underlying mechanisms. We found that CMR dose-dependently inhibited the proliferation of human lung cancer H460, A549 and PC-9 cells. Furthermore, exposure to low and median doses of CMR induced paraptosis but not apoptosis, which was presented as the formation of extensive cytoplasmic vacuolation with increased expression of endoplasmic reticulum stress markers, Bip and Chop, as well as activation of MAPK pathway in the lung cancer cells. Knockdown of Bip with siRNA not only reduced the cell-killing effect of CMR, but also decreased the percentage of cytoplasmic vacuoles in H460 cells. Moreover, CMR also increased the sensitivity of lung cancer cells to radiotherapy through enhanced endoplasmic reticulum stress. In lung cancer H460 cell xenograft nude mice, combined treatment of CMR and radiation caused greatly enhanced tumor growth inhibition with upregulation of endoplasmic reticulum stress proteins and activation of pErk in xenograft tumor tissue. These data demonstrate that the anticancer activity and radiosensitization effect of CMR result from inducing paraptosis, suggesting that CMR could be considered as a potential anticancer agent and radiation sensitizer in the future cancer therapeutics.

Genome-wide profiling reveals alternative polyadenylation of mRNA in human non-small cell lung cancer.

BACKGROUND: Lung cancer is the second most common cancer with an extremely poor overall survival rate. Post-transcriptional regulation of gene expression play many important roles in human cancer, and one of the potential mechanisms underlying this is alternative mRNA maturation at its 3' untranslated regions (3'-UTRs). METHODS: Cancer tissues and paired adjacent normal lung tissues from 26 patients diagnosed with non-small cell lung cancer (NSCLC) were analyzed by in vitro transcription-sequencing alternative polyadenylation sites (IVT-SAPAS). 41,773,101 reads in average were obtained from each paired sample. A potential regulation of Cleavage Stimulation Factor Subunit 2 (CSTF2) on 3'UTR length of genes was tested in H460 cells. RESULTS: 1439 (10.26%) genes showed up-regulated expression and 1364 (9.72%) genes showed down-regulated expression in lung cancer tissue versus normal lung tissue, and shorten 3'UTR in cancer tissue was detected in cancer tissues collected from 96.2% (25/26) patients, indicating lung cancer tend to have shortened 3'UTRs of these identified genes. KEGG analysis showed 1855 genes with shorten 3'UTR were enriched in mTOR signaling, ubiquitin mediated proteolysis and RNA degradation. Knocking down CSTF2 expression in H460 cells results in 3'UTR elongation of genes that was identified to be with shortened length in cancer tissues. CONCLUSION: Alternative polyadenylation (APA) site-switching of 3'UTRs is prevalent in NSCLC, and CSTF2 may serve as an oncogene regulates the 3'UTR length of cancer related genes in NSCLC.

Generalized inverse matrix-exterior penalty function (GIM-EPF) algorithm for data processing of multi-wavelength pyrometer (MWP).

The unknown emissivity of materials is a huge obstacle in multi-wavelength pyrometry (MWP). It leads to a set of ill-posed equations that cannot be directly inverted to obtain the true temperature from a set of multi-wavelength measurements. Constraint optimization algorithms such as the gradient projection (GP) and internal penalty function (IPF) algorithms provide solutions without any emissivity model assumptions but require a narrow fixed emissivity range and an appropriate initial emissivity input value, otherwise, accuracy and computational efficiency are greatly affected. Here, we propose a generalized inverse matrix-exterior penalty function (GIM-EPF) algorithm to realize an efficient and accurate inversion without limiting the emissivity range in advance. First, a set of emissivities is obtained by the generalized inverse matrix method; these emissivities are used as initial values in the exterior penalty function iteration algorithm, from which temperature and spectral emissivity are obtained. Simulation results show that the GIM-EPF algorithm provides results superior to IPF, especially in computational efficiency. The proposed GIM-EPF method is 8 times faster than the IPF method with a 0.56% relative error at the 1800 K true temperature. The GIM-EPF method also allows near real-time diagnosis of rocket exhaust flame temperature. Rocket nozzle temperature experiment results show that the temperatures derived by the GIM-EPF algorithm agree well with the theoretical design temperature and the IPF inversion temperature.

Counterfactual comparison modulates fairness consideration in the mini-ultimatum game: an event-related potentials study.

Existing literature on the mini-ultimatum game indicates that counterfactual comparison between chosen and unchosen alternatives is of great importance for individual's fairness consideration. However, it is still unclear how counterfactual comparison influences the electrophysiological responses to unfair chosen offers. In conjunction with event-related potentials' (ERPs) technique, the current study aimed to explore the issue by employing a modified version of the mini-ultimatum game where a fixed set of two alternatives (unfair offer vs. fair alternative, unfair vs. hyperfair alternative, unfair offer vs. hyperunfair alternative) was presented before the chosen offer. The behavioral results showed that participants were more likely to accept unfair chosen offers when the unchosen alternative was hyperunfair than when the unchosen alternative was fair or hyperfair. The ERPs results showed that the feedback-related negativity (FRN) elicited by unfair chosen offers was insensitive to the type of unchosen alternative when correcting for possible overlap with other components. In contrast, unfair chosen offers elicited larger P300 amplitudes when the unchosen alternative was hyperunfair than when the unchosen alternative was fair or hyperfair. These findings suggest that counterfactual comparison may take effect at later stages of fairness consideration as reflected by the P300.

The priming effects of emotional vocal expressions on face encoding and recognition: An ERP study.

Previous studies have suggested that emotional primes, presented as visual stimuli, influence face memory (e.g., encoding and recognition). However, due to stimulus-associated issues, whether emotional primes affect face encoding when the priming stimuli are presented in an auditory modality remains controversial. Moreover, no studies have investigated whether the effects of emotional auditory primes are maintained in later stages of face memory, such as face recognition. To address these issues, participants in the present study were asked to memorize angry and neutral faces. The faces were presented after a simple nonlinguistic interjection expressed with angry or neutral prosodies. Subsequently, participants completed an old/new recognition task in which only faces were presented. Event-related potential (ERP) results showed that during the encoding phase, all faces preceded by an angry vocal expression elicited larger N170 responses than faces preceded by a neutral vocal expression. Angry vocal expression also enhanced the late positive potential (LPP) responses specifically to angry faces. In the subsequent recognition phase, preceding angry vocal primes reduced early LPP responses to both angry and neutral faces and late LPP responses specifically to neutral faces. These findings suggest that the negative emotion of auditory primes influenced face encoding and recognition.

Current and lasting effects of affect labeling on late positive potential (LPP) amplitudes elicited by negative events.

INTRODUCTION: Labeling the emotional aspect of self-unrelated stimuli (i.e., affect labeling) is a crucial strategy for implicit emotion regulation. However, it is uncertain whether affect labeling influences event-related potential (ERP) responses (e.g., the late positive potential, LPP) to negative stimuli in comparison with control conditions in which attention is shifted to the emotional content of the stimuli (e.g., affect matching). Additionally, it is unknown whether affect labeling has a lasting effect on the processing of negative stimuli. METHODS: Participants were required to label the emotion (negative or neutral) of target pictures with two words, to match the emotion with alternative pictures or to merely view the target pictures. Target pictures were presented again immediately after the regulation task. After all the target pictures had been labeled, matched and viewed, the pictures were re-exposed for the third time. RESULTS: The results showed that negative pictures elicited larger late LPP responses during the affect labeling task than during other tasks. However, the LPP responses were smaller for negative pictures in the affect labeling condition than in the other conditions when target pictures were re-exposed immediately after the task. When target pictures were re-presented again long after the regulation tasks, the LPP responses were smaller for negative stimuli with a history of affect labeling than viewing, whereas this effect did not differ between the affect labeling and matching conditions. CONCLUSION: The current findings suggest that affect labeling has current effects and, to some extent, has lasting effects on negative stimulus processing.

CISD2 promotes lung squamous carcinoma cell migration and invasion via the TGF-ß1-induced Smad2/3 signaling pathway.

BACKGROUND: Although aberrant expression of CDGSH iron sulfur domain 2 (CISD2) contributes to the tumorigenesis and progression of numerous human cancers, the biological function of CISD2 and its specific prognostic value in lung squamous cell carcinoma (LUSC) have yet to be comprehensively explored. The current study aimed to elucidate the role of CISD2 in LUSC as well as the underlying molecular mechanisms. METHODS: Immunohistochemistry was conducted to detect the protein expression of CISD2 and analyze whether high expression of CISD2 affects the overall survival (OS) of LUSC patients. Cell proliferation, colony formation, wound healing and Transwell invasion assays were performed to clarify whether CISD2 contributes to LUSC cell proliferation and disease progression. Quantitative real-time reverse transcription-PCR and western blot assays were used to detect the levels of transcription factors and key epithelial-mesenchymal transition (EMT)-related markers in LUSC cells after CISD2 knockdown and overexpression to determine whether CISD2 regulates transforming growth factor-beta (TGF-ß)-induced EMT in LUSC. RESULTS: Immunohistochemistry of human tissue microarrays containing 90 pairs of adjacent and cancerous tissues revealed that CISD2 is considerably overexpressed in LUSC and strongly linked to poor OS. Functional experiments suggested that silencing endogenous CISD2 inhibited the growth, colony formation, migration, and invasion of H2170 and H226 cell lines. Exogenous overexpression of CISD2 facilitated these phenotypes in SK-MES-1 and H2170 cells. Furthermore, CISD2 promoted EMT progression by increasing the expression of mesenchymal markers (N-cadherin, vimentin, Snail, and Slug) as well as SMAD2/3 and reducing the expression of the epithelial marker E-cadherin. Mechanistically, our studies provide the first evidence that CISD2 can promote EMT by enhancing TGF-ß1-induced Smad2/3 expression in LUSC cells. CONCLUSION: In conclusion, our research illustrates that CISD2 is highly expressed in LUSC and may facilitate LUSC proliferation and metastasis. Thus, CISD2 may serve as an independent prognostic marker and possible treatment target for LUSC.

The second-generation tyrosine kinase inhibitor afatinib inhibits IL-1ß secretion via blocking assembly of NLRP3 inflammasome independent of epidermal growth factor receptor signaling in macrophage.

Chronic inflammation might lead to many malignancies, and inadequate resolution could play a crucial role in tumor invasion, progression and metastases. Afatinib is a second-generation tyrosine kinase inhibitor targeting epidermal growth factor receptor in non-small cell lung cancer. Few studies showed the correlation of afatinib and the innate immune system especially macrophage. Our study showed that afatinib could block the activation of NLRP3 inflammasome in a dose-dependent manner in macrophage, and that afatinib could prevent the assembly of NLRP3 inflammasome. Besides, afatinib could inhibit NLRP3 inflammasome activation independent of EGFR signaling. Moreover, afatinib was able to alleviate the LPS-induced sepsis in vivo. These investigations provide significant experimental evidence in afatinib as therapeutic drug for non-small cell lung cancer or other tumors and NLRP3-related diseases, and explore new target for afatinib in macrophage.

ERP Effects of Malicious Envy on Schadenfreude in Gain and Loss Frames.

Previous behavioral and neural studies have shown the effects of malicious envy on schadenfreude. However, it is unclear whether these effects are modulated by contextual frames (e.g., gain and loss frames). Thus, the present study aimed to investigate whether behavioral and event-related potential (ERP) effects of malicious envy on schadenfreude were different in gain and loss frames. To address this issue, the participants in the present study believed they were playing a monetary game with several other players. In the malicious envy condition, the participants won less money than the player in the gain frame and lost more money in the loss frame; in the control condition, both participants and the player gained little money in the gain frame and lost much in the loss frame. Subsequently, the participants were informed that the player encountered a misfortune, i.e., gained little in the gain frame and lost much in the loss frame. Results showed that malicious envy increased feelings of schadenfreude and ERP responses when the player encountered a misfortune. Moreover, increased ERP responses by malicious envy occurred at the feedback-related negativity (FRN), and early late positive potential (LPP) time ranges in the gain frame but at the late LPP time range in the loss frame. The findings might suggest that malicious envy affects schadenfreude and corresponding neural activity, whereas the neural effects occur at comparatively early time ranges in the gain frame but at a later time range in the loss frame.

The Effect of Malicious Envy on Schadenfreude When Schadenfreude Is Elicited Through Social Comparisons.

Previous studies have investigated whether envy, particularly malicious envy, increases feelings of schadenfreude and whether this effect is evident in both gain and loss frames. However, as a social-comparison-based emotion, schadenfreude was not investigated through social comparisons in these previous studies. Thus, the present study aimed to investigate whether malicious envy influences schadenfreude when schadenfreude is elicited in the context of precise and ambiguous social comparisons. To address this issue, participants in the present study were asked to play a monetary game with several other players. In the experimental condition, participants gained less or lost more than the other player; in the control condition, both the participants and the player gained little or lost much. Subsequently, the participants observed that the player encountered a misfortune, that is, gained less or lost more money than the participant. The results showed that when participants knew the exact amount of monetary gained and lost by themselves and the other player (i.e., precise social comparisons), malicious envy increased feelings of schadenfreude only in the loss frame rather than in the gain frame. More importantly, malicious envy turned out to reduce feelings of schadenfreude in both gain and loss frames, when participants did not know the exact amount (i.e., ambiguous social comparisons). The findings provide novel evidence that malicious envy does not always increase schadenfreude particularly when schadenfreude is elicited through social comparisons.

Working memory load reduces the processing of outcome evaluation involving others but not oneself: Event-related potential evidence.

Previous event-related potential (ERP) studies have suggested that self-related and non-self-related outcomes are processed simultaneously. However, the studies investigated situations in which individuals had sufficient attentional/cognitive resources to process both of the outcomes. It is unknown whether self-related and non-self-unrelated outcomes could still be processed simultaneously when resources are limited. To address this issue, 32 female participants in the present study were asked to perform a working memory task. To manipulate the amount of available attentional/cognitive resources, participants were asked to memorize a letter in the low load condition and five letters in the high load condition. During letter consolidation, participants were informed that they and another player each performed a gambling task and were subsequently presented with both of the outcomes. ERP results showed that others' monetary loss elicited larger P200 and late positive potential amplitudes than others' monetary gain under a low working memory load, whereas a high load reduced these effects. However, working memory load did not influence the effect of self-outcome on ERP responses. Therefore, the findings suggest that the amount of available attentional/cognitive resources alters the evaluation of non-self-related but not self-related outcomes.

Integrated Analysis of Genomic and Immunological Features in Lung Adenocarcinoma With Micropapillary Component.

BACKGROUND: Micropapillary adenocarcinoma is one of the most aggressive histologic subtypes of lung adenocarcinoma (LADC), and even a minor proportion of micropapillary component (MPC) within the LADC could contribute to poor prognosis. Comprehensive analysis of genetic and immunological features of LADC with different percentages of MPC would help better understand cancer biology of this LADC subtype and direct future treatments. METHODS: We performed next-generation sequencing (NGS) for a discovery cohort of 43 LADC patients whose tumors were micro-dissected to separate MPC and non-MPC lesions and a reference cohort of 113 LADC patients. MPC-enriched genetic alterations that were detected in the discovery cohort were then confirmed using a validation cohort of 183 LADC patients. Immunological staining was also conducted on the MPC-containing samples in the discovery cohort. RESULTS: Tumors with a higher percentage of MPC tended to harbor more tumor mutation burdens (TMBs) and chromosome instability (CIN). Some rare genetic events may serve as the genetic landscape to drive micropapillary tumor progression. Specifically, alterations in transcription termination factor 1 (TTF1), brain-specific angiogenesis inhibitor 3 (BAI3), mammalian target of rapamycin (MTOR), and cyclin-dependent kinase inhibitor 2A (CDKN2A) were cross-validated to be enriched in MPC-contained LADC. Additionally, tumors with a higher percentage of MPC were associated with a higher percentage of CD4+, CD8+, and PD-L1+ staining, and some genetic changes that were enriched in MPC, including MET amplification and MTOR mutation, were correlated with increased PD-L1 expression. CONCLUSION: We identified multiple novel MPC-enriched genetic changes that could help us understand the nature of this aggressive cancer subtype. High MPC tumors tended to have elevated levels of TMBs, T cell infiltration, and immunosuppression than low MPC tumors, implying the potential link between MPC content and sensitivity to immunotherapy.

Negative expectations influence behavioral and ERP responses in the subsequent recognition of expectancy-incongruent neutral events.

Previous studies have shown that expectancy incongruence in emotional stimuli influences the encoding (i.e., the first stage of memory processing) of the stimuli. However, it is unknown about whether expectancy incongruence influences later stages of memory processing, such as recognition. To this end, expectancy cues were presented prior to emotional pictures. Most often, the cues accurately indicated the emotional consequences of the pictures, but in some cases the consequence was incongruent with the expectations, and a picture from another emotional category was presented. Afterward, participants completed an unexpected recognition task in which old and novel pictures were not preceded by expectancy cues. The results showed that, in the encoding phase, expectancy incongruence reduced response accuracy when categorizing pictorial emotions, and the effect was smaller for neutral pictures than for negative pictures. ERP results showed stronger and weaker responses to expectancy incongruent pictures compared to congruent pictures in time ranges related to the encoding-related early and middle late positive potential (LPP), respectively. In the subsequent recognition phase, d' scores were higher for incongruent neutral pictures than for congruent ones. Expectancy incongruence enlarged the P2 response but reduced the recognition-related early LPP response for neutral pictures. However, effects of expectancy incongruence were not seen for negative pictures. Therefore, the findings in the present study indicate that negative expectations influence the later recognition of expectancy incongruent neutral events, whereas negative events are more resistant to the effects of expectation incongruence.