Relationship among antimutagenic, antioxidant and enzymatic activities of methanolic extract from common beans (Phaseolus vulgaris L).

Common beans are rich in phenolic compounds, which can provide health benefits to the consumer. The objective of this work was to study the relationship among antimutagenicity, antioxidant and enzymatic activities of methanolic extract and trolox by principal components multivariate analysis. Antimutagenicity of phenolic compounds present in methanolic extract from the seed coat of common beans (P. vulgaris, Flor de Mayo Bajío cultivar) and trolox against AFB1 mutagenicity were evaluated in the Salmonella typhimurium microsuspension assay. Antioxidant capacity of methanolic extract and trolox were evaluated using beta-carotene and 1,1-diphenyl-2-picryhydrazyl (DPPH) in vitro model assays. Cythrome P450 activity was measured by fluorometric assay. For phenolic extract, trolox and phenolic extract+trolox, the inhibition on AFB1 mutagenicity in tester strain TA100 was 47, 59 and 69%, respectively. While in TA98 was 39, 48 and 68%. The inhibition of phenolic compounds, trolox and phenolic compounds+trolox on cytochrome P450 (CYP450) activity was 48, 59 and 88%, respectively. Correlation analysis showed that phenolic extract and trolox have high antimutagenic and antioxidant activity and also inhibited enzymatic activity. The results suggest that the primary mechanism of action of phenolic compounds in beans against AFB1 mutagenicity may be extra-cellular in the microsuspension assay.

Causas del color amarillo de la grasa de canales de bovinos finalizados en pastoreo / Causes of yellow fat deposited in carcasses of bovines finished on pasture

En México la mayor parte de los bovinos productores de carne son finalizados en pastoreo. Se ha observado que el tejido adiposo de estos animales puede presentar una coloración cuasi amarilla, que provoca su rechazo por parte del consumidor y la consecuente pérdida económica para el productor. El color casi amarillo de la grasa en bovinos se debe a los carotenoides provenientes de su ingesta. En esta revisión se aborda el metabolismo de estos compuestos en los ámbitos digestivo y tisular. Aunque se conocen algunas soluciones prácticas al problema, éstas son económicamente inviables; en este contexto, es necesario estudiar más sobre las causas fisiológicas de la deposición del pigmento con el propósito de encontrar mejores soluciones.