Non-infectious immune complexes downregulate the production of interferons and tumor necrosis factor-α in primary porcine alveolar macrophages in vitro.

Porcine reproductive and respiratory syndrome (PRRS) caused by the PRRS virus (PRRSV) has been harming the pig industry worldwide for nearly 40 years. Although scientific researchers have made substantial efforts to explore PRRSV pathogenesis, the immune factors influencing PRRSV infection still need to be better understood. Infectious virus-antibody immune complexes (ICs) formed by PRRSV and sub-or non-neutralizing antibodies specific for PRRSV may significantly promote the development of PRRS by enhancing PRRSV replication through antibody-dependent enhancement. However, nothing is known about whether PRRSV infection is affected by non-infectious ICs (NICs) formed by non-pathogenic/infectious antigens and corresponding specific antibodies. Here, we found that PRRSV significantly induced the transcripts and proteins of interferon-α (IFN-α), IFN-ß, IFN-γ, IFN-λ1, and tumor necrosis factor-α (TNF-α) in vitro primary porcine alveolar macrophages (PAMs) in the early stage of infection. Our results showed that NICs formed by rabbit-negative IgG (RNI) and pig anti-RNI specific IgG significantly reduced the transcripts and proteins of IFN-α, IFN-ß, IFN-γ, IFN-λ1, and TNF-α in vitro PAMs and significantly elevated the transcripts and proteins of interleukine-10 (IL-10) and transforming growth factor-ß1 (TGF-ß1) in vitro PAMs. NICs-mediated PRRSV infection showed that NICs not only significantly decreased the induction of IFN-α, IFN-ß, IFN-γ, IFN-λ1, and TNF-α by PRRSV but also significantly increased the induction of IL-10 and TGF-ß1 by PRRSV and considerably enhanced PRRSV replication in vitro PAMs. Our data suggested that NICs could downregulate the production of antiviral cytokines (IFN-α/ß/γ/λ1 and TNF-α) during PRRSV infection in vitro and facilitated PRRSV proliferation in its host cells by inhibiting innate antiviral immune response. This study elucidated one novel immune response to PRRSV infection, which would enhance our understanding of the pathogenesis of PRRSV.

Investigation of arsenic contamination in soil and plants along the river of Xinzhou abandoned gold mine in Qingyuan, China.

The exploitation of mineral resources is very important for economic development, but disorderly exploitation poses a serious threat to the ecological environment. However, investigations on the advantages of plant species and environmental pollution in polluted mining areas are limited. Thus, a survey was conducted to evaluate the impacts of abandoned mines on the surrounding ecological environment along rivers in polluted areas and to determine the Arsenic (As) pollution status in soil and plants. The results showed that the soil and vegetation along the river in the survey area were seriously polluted by As. The total As content of the 15 samples was significantly greater than the national soil background value (GB 15618-2018), and degree of pollution was nonlinearly related to the distance from the mine source, R2 = 0.9844. B. bipinnata, P. vittata and B. nivea were predominant with degrees of dominance of 0.01-0.33, 0.05-0.11, and 0.06-0.14 respectively. The As enrichment capacities of Juncus and P. vittata were significantly greater than those of the other plants, while the bioaccumulation factors (BCFs) were 21.81 and 7.04, respectively.

Effect of early cannulation with metal needles on arteriovenous fistula patency in patients undergoing hemodialysis.

BACKGROUND: Autogenous arteriovenous fistula (AVF) is the preferred vascular access mode. However, the earliest possible time for AVF puncture and whether premature puncture affects the AVF patency rate remain unclear. METHODS: In this multicenter retrospective cohort study, adult uremic patients who underwent AVF surgery for the first time at Taizhou Hospital or Enze Hospital of Zhejiang Province between September 1, 2018 and August 31, 2021 were enrolled. All patients were followed up for 1 year after puncture, and the status of fistula establishment and puncture, subsequent patency, loss to follow-up, renal transplantation, conversion to peritoneal dialysis, abandonment of the fistula, and death, were recorded. RESULTS: A total of 465 patients with AVFs were included in this study, including 59 (12.7%) patients with fistulas that were cannulated within 30 days. In the early puncture group, the levels of serum creatinine and urea nitrogen were higher, while the levels of hemoglobin and albumin were lower, suggesting that these patients needed urgent dialysis. Furthermore, the rate of non-cuffed catheter use was higher, while the rate of cuffed catheter use was lower, and femoral vein puncture was preferred over internal jugular vein puncture. The mean duration of catheter indwelling was shorter in the early puncture group (19 vs 70 days, p < 0.001). The estimated AVF primary and cumulative functional patency at 12 months was 81.1% versus 82.3% and 98.3% versus 98.7% in the early puncture and control groups, respectively. Kaplan-Meier analysis revealed no significant difference in AVF primary and cumulative functional patency between the two groups. CONCLUSIONS: In patients with an established fistula in urgent need of hemodialysis, to avoid new catheterization, a puncture can be performed within 30 days in those with well-developed blood vessels after adequate ultrasound and clinical evaluation without affecting the patency of the fistula.

Boosting anaerobic digestion of long chain fatty acid with microbial electrolysis cell combining metal organic framework as cathode: Biofilm construction and metabolic pathways.

The role of metal organic framework (MOF) modified cathode in promoting long chain fatty acid (LCFA) methanation was identified in microbial electrolysis cell coupled anaerobic digestion (MEC-AD) system. The maximum methane production rate of MEC-AD-MOF achieved 49.8 ± 3.4 mL/d, which increased by 41 % compared to MEC-AD-C. The analysis of bio-cathode biofilm revealed that microbial activity, distribution, population, and protein secretion prompted by MOF cathode, which in turn led to an acceleration of electron transfer between the cathode and microbes. Specifically, the relative abundance of acetate-oxidizing bacterium (Mesotoga) in MEC-AD-MOF was 1.5-3.6 times higher than that in MEC-AD-C, with a co-metabolized enrichment of Methanobacterium. Moreover, MOF cathode reinforced LCFA methanation by raising the relative abundance of genes coded key enzymes involved in CO2-reducing pathway, and elevating the tolerance of microbes to LCFA inhibition. These results indicate that MOF can enhance biofilm construction in MEC-AD, thereby improving the treatment performance of lipid wastewater.

Self-Assembled Monolayer and Nanoparticles Coenhanced Fragmented Silver Nanowire Network Memristor.

Silver nanowire (AgNW) networks with self-assembled structures and synaptic connectivity have been recently reported for constructing neuromorphic memristors. However, resistive switching at the cross-point junctions of the network is unstable due to locally enhanced Joule heating and the Gibbs-Thomson effect, which poses an obstacle to the integration of threshold switching and memory function in the same AgNW memristor. Here, fragmented AgNW networks combined with Ag nanoparticles (AgNPs) and mercapto self-assembled monolayers (SAMs) are devised to construct memristors with stable threshold switching and memory behavior. In the above design, the planar gaps between NW segments are for resistive switching, the AgNPs act as metal islands in the gaps to reduce threshold voltage (Vth) and holding voltage (Vhold), and the SAMs suppress surface atom diffusion to avoid Oswald ripening of the AgNPs, which improves switching stability. The fragmented NW-NP/SAM memristors not only circumvent the side effects of conventional NW-stacked junctions to provide durable threshold switching at >Vth but also exhibit synaptic characteristics such as long-term potentiation at ultralow voltage (≪Vth). The combination of NW segments, nanoparticles, and SAMs blazes a new trail for integrating artificial neurons and synapses in AgNW network memristors.

ddPCR provides a sensitive test compared with GeneXpert MTB/RIF and mNGS for suspected Mycobacterium tuberculosis infection.

Introduction: The Metagenomics next-generation sequencing (mNGS) and GeneXpert MTB/RIF assay (Xpert) exhibited a sensitivity for tuberculosis (TB) diagnostic performance. Research that directly compared the clinical performance of ddPCR analysis, mNGS, and Xpert in mycobacterium tuberculosis complex (MTB) infection has not been conducted. Methods: The study aimed to evaluate the diagnostic performance of ddPCR compared to mNGS and Xpert for the detection of MTB in multiple types of clinical samples. The final clinical diagnosis was used as the reference standard. Results: Out of 236 patients with suspected active TB infection, 217 underwent synchronous testing for tuberculosis using ddPCR, Xpert, and mNGS on direct clinical samples. During follow-up, 100 out of 217 participants were diagnosed with MTB infection. Compared to the clinical final diagnosis, ddPCR produced the highest sensitivity of 99% compared with mNGS (86%) and Xpert (64%) for all active MTB cases. Discussion: Twenty-two Xpert-negative samples were positive in mNGS tests, which confirmed the clinical diagnosis results from ddPCR and clinical manifestation, radiologic findings. Thirteen mNGS-negative samples were positive in ddPCR assays, which confirmed the clinical final diagnosis.ddPCR provides a higher sensitive compared to Xpert and mNGS for MTB diagnosis, as defined by the high concordance between ddPCR assay and clinical final diagnosis.

Breaking Surface Atomic Monogeneity of Rh2 P Nanocatalysts by Defect-Derived Phosphorus Vacancies for Efficient Alkaline Hydrogen Oxidation.

Breaking atomic monogeneity of catalyst surfaces is promising for constructing synergistic active centers to cope with complex multi-step catalytic reactions. Here, we report a defect-derived strategy for creating surface phosphorous vacancies (P-vacancies) on nanometric Rh2 P electrocatalysts toward drastically boosted electrocatalysis for alkaline hydrogen oxidation reaction (HOR). This strategy disrupts the monogeneity and atomic regularity of the thermodynamically stable P-terminated surfaces. Density functional theory calculations initially verify that the competitive adsorption behavior of Had and OHad on perfect P-terminated Rh2 P{200} facets (p-Rh2 P) can be bypassed on defective Rh2 P{200} surfaces (d-Rh2 P). The P-vacancies enable the exposure of sub-surface Rh atoms to act as exclusive H adsorption sites. Therein, the Had cooperates with the OHad on the peripheral P-sites to effectively accelerate the alkaline HOR. Defective Rh2 P nanowires (d-Rh2 P NWs) and perfect Rh2 P nanocubes (p-Rh2 P NCs) are then elaborately synthesized to experimentally represent the d-Rh2 P and p-Rh2 P catalytic surfaces. As expected, the P-vacancy-enriched d-Rh2 P NWs catalyst exhibits extremely high catalytic activity and outstanding CO tolerance for alkaline HOR electrocatalysis, attaining 5.7 and 14.3 times mass activity that of p-Rh2 P NCs and commercial Pt/C, respectively. This work sheds light on breaking the surface atomic monogeneity for the development of efficient heterogeneous catalysts.

The application of mixed stabilizing materials promotes the feasibility of the intercropping system of Gynostemma pentaphyllum/Helianthus annuus L. on arsenic contaminated soil.

Intercropping technology and stabilizing materials are common remediation techniques for soils contaminated with heavy metals. This study investigated the feasibility of the Gynostemma pentaphyllum (G. pentaphyllum)/Helianthus annuus L. (H. annuus) intercropping system on arsenic (As) contaminated farmland through field and pot experiments and the regulation of plant As absorption by the application of mixed stabilizing materials in this intercropping system. Field experiments demonstrated that intercropping with H. annuus increased the As concentration in G. pentaphyllum leaves to 1.79 mg kg-1 but still met the requirements of the national food standard of China (2 mg kg-1) (GB2762-2017). Meanwhile, G. pentaphyllum yield in the intercropping system decreased by 15.09%, but the difference was insignificant (P > 0.05). Additionally, the As bioconcentration (BCA) per H. annuus plant in the intercropping system was significantly higher than that in the monoculture system, increasing by 76.37% (P < 0.05). The pot experiment demonstrated that when granite powder, iron sulfate mineral, and "Weidikang" soil conditioner were applied to the soil collectively, G. pentaphyllum leaf As concentration in the intercropping system could be significantly reduced by 42.17%. Rhizosphere pH is the most crucial factor affecting As absorption by G. pentaphyllum in intercropping systems. When these three stabilizing materials were applied simultaneously, the As bioaccumulation (BCA) per H. annuus plant was significantly higher than that of normal intercropping treatment, which increased by 71.12% (P < 0.05), indicating that the application of these stabilizing materials significantly improved the As removal efficiency of the intercropping system. Dissolved organic carbon (DOC) concentration in the rhizosphere soil is the most pivotal factor affecting As absorption by H. annuus. In summary, the G. pentaphyllum-H. annuus intercropping model is worthy of being promoted in moderately As polluted farmland. The application of granite powder, iron sulfate mineral, and "Weidikang" soil conditioner collectively to the soil can effectively enhance the potential of this intercropping model to achieve "production while repairing" in the As polluted farmland.

Antioxidative and antibacterial gallium (III)-phenolic coating for enhanced osseointegration of titanium implants via pro-osteogenesis and inhibiting osteoclastogenesis.

Improving the ability of implants to integrate with natural bone tissue at the initial stage of implantation remains a huge challenge because bone-to-implant interfaces are often accompanied by abnormal microenvironments with infection, reactive oxygen species (ROS) and unbalanced bone homeostasis. In this study, a multifunctional coating was fabricated on the basis of gallium (III)-phenolic networks. It is easily obtained by immersing the implants into a mixed solution of tannic acids (TAs) and gallium ions. The thickness of the coating can be precisely controlled by adjusting the number and time of immersion experiments. The resulting coating displays excellent near-infrared photothermal property. As the coating degrades, TAs and gallium ions with low concentration are released from the coating, which is more rapid in acidic and oxidative stress microenvironments. Photothermal performance as well as released TAs and gallium ions give the coating outstanding broad-spectrum antibacterial ability. Furthermore, the coating effectively reduces intracellular ROS of osteoblasts. In vitro and in vivo experiments demonstrate the capability of the coating enhancing implants' osseointegration via pro-osteogenesis and inhibiting osteoclastogenesis. The findings imply that gallium (III)-phenolic coating holds great promise to promote implant osseointegration by rescuing abnormal microenvironments of infection, oxidative stress and unbalanced bone homeostasis.

Platelet Rich Plasma Loaded Multifunctional Hydrogel Accelerates Diabetic Wound Healing via Regulating the Continuously Abnormal Microenvironments.

Continuous oxidative stress and cellular dysfunction caused by hyperglycemia are distinguishing features of diabetic wounds. It has been a great challenge to develop a smart dressing that can accelerate diabetic wound healing through regulating abnormal microenvironments. In this study, a platelet rich plasma (PRP) loaded multifunctional hydrogel with reactive oxygen species (ROS) and glucose dual-responsive property is reported. It can be conveniently prepared with PRP, dopamine (DA) grafted alginate (Alg-DA), and 6-aminobenzo[c][1,2]oxaborol-1(3H)-ol (ABO) conjugated hyaluronic acid (HA-ABO) through ionic crosslinks, hydrogen-bond interactions, and boronate ester bonds. The hydrogel possesses injectability, moldability, tissue adhesion, self-healing, low hemolysis, and hemostasis performances. Its excellent antioxidant property can create a low oxidative stress microenvironment for other biological events. Under an oxidative stress and/or hyperglycemia state, the hydrogel can degrade at an accelerated rate to release a variety of cytokines derived from activated blood platelets. The result is a series of positive changes that are favorable for diabetic wound healing, including fast anti-inflammation, activated macrophage polarization toward M2 phenotype, promoted migration and proliferation of fibroblasts, as well as expedited angiogenesis. This work provides an efficient strategy for chronic diabetic wound management and offers an alternative for developing a new-type PRP-based bioactive wound dressing.

Dynamic associations between the respiratory tract and gut antibiotic resistome of patients with COVID-19 and its prediction power for disease severity.

The antibiotic resistome is the collection of all antibiotic resistance genes (ARGs) present in an individual. Whether an individual's susceptibility to infection and the eventual severity of coronavirus disease 2019 (COVID-19) is influenced by their respiratory tract antibiotic resistome is unknown. Additionally, whether a relationship exists between the respiratory tract and gut ARGs composition has not been fully explored. We recruited 66 patients with COVID-19 at three disease stages (admission, progression, and recovery) and conducted a metagenome sequencing analysis of 143 sputum and 97 fecal samples obtained from them. Respiratory tract, gut metagenomes, and peripheral blood mononuclear cell (PBMC) transcriptomes are analyzed to compare the gut and respiratory tract ARGs of intensive care unit (ICU) and non-ICU (nICU) patients and determine relationships between ARGs and immune response. Among the respiratory tract ARGs, we found that Aminoglycoside, Multidrug, and Vancomycin are increased in ICU patients compared with nICU patients. In the gut, we found that Multidrug, Vancomycin, and Fosmidomycin were increased in ICU patients. We discovered that the relative abundances of Multidrug were significantly correlated with clinical indices, and there was a significantly positive correlation between ARGs and microbiota in the respiratory tract and gut. We found that immune-related pathways in PBMC were enhanced, and they were correlated with Multidrug, Vancomycin, and Tetracycline ARGs. Based on the ARG types, we built a respiratory tract-gut ARG combined random-forest classifier to distinguish ICU COVID-19 patients from nICU patients with an AUC of 0.969. Cumulatively, our findings provide some of the first insights into the dynamic alterations of respiratory tract and gut antibiotic resistome in the progression of COVID-19 and disease severity. They also provide a better understanding of how this disease affects different cohorts of patients. As such, these findings should contribute to better diagnosis and treatment scenarios.

Experimental Study on Bond Performance of NC-UHPC Interfaces with Different Roughness and Substrate Strength.

This paper investigates the bond failure performance between precast normal concrete (NC) and cast-in situ ultra-high performance concrete (UHPC), emphasizing the influence of interfacial roughness. The interfacial bonding behavior under tension and under shear was investigated based on 72 groups of pull-off tests and 36 groups of bi-shear tests, considering six different interface treatment methods and two different NC strength levels. The results demonstrate that certain interfacial roughness is essential to gain a reliable bond connection between NC and UHPC. Its enhancement on the tensile bond performance could be described by the positive linear relationship between the mean roughness and the pull-off strength. However, further research is required to determine the characterization method of its influence on the shear bond performance. The higher strength of the base concrete is beneficial to the bond performance. Though this effect is evident in the pull-off tests under tension, the influence on the shear interfacial performance could be offset by that of the interface morphology in the case with high interfacial roughness.

Effects of different planting distances and fertilizer use on the remediation of farmland contaminated with Cd by intercropping Cucurbita moschata and Amaranthus hypochondriacus L.

Selecting suitable agronomic measures can strengthen the application of intercropping in the remediation of cadmium (Cd)-contaminated soil. In this study, the effects of different planting densities and fertilizer applications on the crop growth and Cd absorption of a pumpkin (Cucurbita moschata)-Amaranthus hypochondriacus L. intercropping system was determined. The goal was to provide enhanced means and a scientific basis for the promotion and application of this intercropping system. The Cd content of pumpkin in different planting systems was lower than the national food safety standard (0.05 mg kg-1). In the IN-1 (4 pumpkin plants intercropped with 200 A. hypochondriacus plants) and IN-2 (4 pumpkin plants intercropped with 400 A. hypochondriacus plants) intercropping systems, the bioconcentration amount (BCA) per plant of Cd in A. hypochondriacus increased by 32.43% and 25.25%, respectively, compared with that of the monocropping system (P < 0.05). The IN-2 system had the highest equivalent ratio of heavy metal removal (3.08), indicating that this model had a substantial advantage for removing Cd. The land equivalent ratio of IN-1 (2.89) and IN-2 (2.60) was significantly higher than that of other intercropping systems, indicating that these two models had obvious yield advantages. In our study, chicken manure was the best at promoting the growth and yield of the two plants and sludge treatment significantly enhance Cd absorption of A. hypochondriacus. In general, intercropping four pumpkin plants with 400 A. hypochondriacus plants and applying chicken manure fertilizer can strengthen the application of this intercropping system in Cd-contaminated soil.

1D group V-VI-VII ternary nanowires: moderate band gaps, easy to exfoliate from bulk, and unexpected ferroelectricity.

One-dimensional nanowires have emerged as compelling ideal materials due to their characteristic structure, properties, and applications in nanodevices. Herein, based on experimental vdW-chain bulk crystals, a series of one-dimensional (1D) XVYVIZVII (X = As, Sb, Bi; Y = S, Se, Te; Z = Cl, Br, I) ternary nanowires are theoretically investigated. Such exfoliated 1D nanowires possess excellent stability and moderate band gaps (1.76-3.16 eV). The calculated electron mobilities were found to reach a magnitude of 102 cm2 V-1 s-1 and even up to 322.95 cm2 V-1 s-1 for 1D BiSeI nanowires, which are much larger than those of the previously reported 1D materials. Furthermore, the appropriate band edge alignments and considerable optical absorption endow 1D XVYVIZVII nanowires with prospective photocatalytic properties for water splitting. Notably, AsSI and AsSeI nanowires possess a unique non-centrosymmetric structure and exhibit promising 1D ferroelectricity. Large spontaneous polarization values, Ps, of 11.31 × 10-10 and 6.92 × 10-10 C m-1 are obtained for 1D AsSI and AsSeI nanowires, respectively, and such 1D ferroelectricity can be regulated by intra-chain strains. Our calculations not only broaden the family of 1D materials but also reveal their great potential applications in electronic, optoelectronic, and ferroelectric devices.

Fast Determination of Rutin on a Biosensor Made Using a Layered Double Hydroxide Nanocomposite Modified Electrode.

In this study, a nanocomposite of LDH/graphene/polyaniline/gold (LDH/rGO/PANI/Au) was synthesized and characterized. The results of characterization showed that the composite material preserved the layered structure of LDH. The composite was dropped onto the glassy carbon electrode and laccase was then immobilized. Electrochemical tests showed that the composite could accelerate the electron transfer between the enzyme and the electrode. The composite/laccase showed an obvious response to rutin and the optimal detection conditions were discussed. The oxidative peak current of the biosensor constructed using the modified electrode was negatively correlated with rutin in the range of 0.05-4 µg/mL. The detection limit was 0.0017 µg/mL at a signal-to-noise ratio of 3. This biosensor of rutin also possessed high sensitivity, excellent anti-interference ability, and stability. The contents of rutin in tablets, first determined using HPLC, were also detected using the sensor constructed in this research as an application, and the results were acceptable. This research here provides a facile way for the fast detection of rutin in real samples.

Weighted gene co-expression network reveals driver genes contributing to phenotypes of anaplastic thyroid carcinoma and immune checkpoint identification for therapeutic targets.

Background: Anaplastic thyroid carcinoma (ATC) is a rare but extremely malignant tumor, with a rapid growth rate and early metastasis thus leading to poor survival of patients. The molecular mechanisms underlying these aggressive traits of ATC remain unknown, which impedes the substantial progress in treatment to prolong ATC patient survival. Methods: We applied weighted gene co-expression network analysis (WGCNA) to identify ATC-specific modules. The Metascape web and R package clusterProfiler were employed to perform enrichment analysis. Combined with differentially expressed gene analysis, we screened out the most potential driver genes and validated them using receiver operator characteristic (ROC) analysis, quantitative reverse transcription polymerase chain reaction (qRT-PCR), western blotting, immunohistochemistry (IHC), and triple immunofluorescence staining. Results: A gene expression matrix covering 75 normal samples, 83 papillary thyroid carcinoma (PTC), 26 follicular thyroid carcinoma (FTC), 19 poor-differentiated thyroid carcinoma (PDTC), and 41 ATC tissue samples were integrated, based on which we detected three most potential ATC-specific modules and found that hub genes of these modules were enriched in distinct biological signals. Hub genes in the turquoise module were mainly enriched in mitotic cell cycle, tube morphogenesis, and cell differentiation, hub genes in the magenta module were mainly clustered in the extracellular matrix organization, positive regulation of cell motility, and regulation of Wnt signaling pathway, while hub genes in the blue module primarily participated in the inflammatory response, innate immune response, and adaptive immune response. We showed that 9 top genes, 8 transcription factors (TFs), and 4 immune checkpoint genes (ICGs) were differentially expressed in ATC compared to other thyroid samples and had high diagnostic values for ATC, among which, 9 novel ATC-specific genes (ADAM12, RNASE2, CASP5, KIAA1524, E2F7, MYBL1, SRPX2, HAVCR2, and TDO2) were validated with our clinical samples. Furthermore, we illustrated that ADAM12, RNASE2, and HAVCR2 were predominantly present in the cytoplasm. Conclusion: Our study identified a set of novel ATC-specific genes that were mainly related to cell proliferation, invasion, metastasis, and immunosuppression, which might throw light on molecular mechanisms underlying aggressive phenotypes of ATC and provide promisingly diagnostic biomarkers and therapeutic targets.

Luffa cylindrica Intercropping with Semen cassiae-A Production Practice of Improving Land Use in Soil Contaminated with Arsenic.

In recent years, research on the safe utilization and green remediation of contaminated soil by intercropping has become common. In this study, the growth of an intercropping system of Luffa cylindrica-Semen cassiae in soil contaminated with medium amounts of arsenic (As) was studied using field (91.60 mg kg-1) and pot (83.34 mg kg-1) experiments. The field experiments showed that intercropping significantly increased the yield per plant of L. cylindrica by 27.36%, while the yield per plant of S. cassiae decreased by 21.66%; however, this difference was not significant. Intercropping reduced the concentration of As in all organs of L. cylindrica but increased the concentration of As in all parts of S. cassiae. The accumulation of As per plant of L. cylindrica was reduced by 20.72%, while that in a single plant of S. cassiae was increased by 201.93%. In addition, the concentration of As in the fruit of these two crops in these two planting modes was low enough to meet the National Food Safety Standard of China (GB2762-2017). In addition, the land equivalent ratio and As metal removal equivalent ratio of the intercropping mode was 1.03 and 2.34, indicating that the intercropping mode had advantages in land use and As removal. In the pot experiment, the biomass and As concentration of L. cylindrica and S. cassiae were roughly consistent with those in the field experiment. During the sampling period, intercropping reduced the concentration of As in the rhizosphere soil solution of L. cylindrica by 3.1-23.77%, while it increased the concentration of As in the rhizosphere soil solution of S. cassiae by 13.30-59.40%. The changes in pH and redox potential were also closely related to the content of water-soluble As in the rhizosphere environment, which affects the absorption of As by plants. In general, the L. cylindrica-S. cassiae intercropping system is a planting mode that can effectively treat soil that is moderately contaminated with As and remove it from the soil to an extent.

Is technetium-99m dimercaptosuccinic acid renal scintigraphy available for predicting vesicoureteral reflux in children with first febrile urinary tract infection under the age of 24 months?

OBJECTIVE: Vesicoureteral reflux (VUR) is a common complication after urinary tract infection (UTI) and can lead to irreversible renal scar. Voiding cystourethrogram is the most reliable technology to detect VUR and its severity, but it is restricted in children's examinations for various shortcomings. This study aimed to evaluate and compare the efficiency of Tc-99m DMSA renal scintigraphy and conventional ultrasonography (USG) in predicting VUR with the gold standard of cystourethrogram results. METHODS: This retrospective study consisted of 285 first febrile UTI children under the age of 24 months who completed inflammatory indicator examinations, USG, Tc-99m DMSA renal scintigraphy and underwent cystourethrography after controlling infection with prophylactic antibiotics. The efficiency of Tc-99m DMSA renal scintigraphy and USG in predicting VUR was calculated and compared. RESULTS: Abnormal USG (40.23% vs. 21.72%, P = 0.001) and Tc-99m DMSA renal scintigraphy results (87.36% vs. 71.72%, P = 0.004) were more common in VUR children. The sensitivity of USG in predicting VUR was only 40.23%, whereas the sensitivity and negative predictive value of Tc-99m DMSA renal scintigraphy reached 87.63 and 83.58%, respectively. Tc-99m DMSA renal scintigraphy had a higher efficacy than USG in predicting high-grade reflux kidneys (73.87% vs. 33.33%; P < 0.001), but there was no significant difference in predicting low-grade reflux kidneys ( P = 0.703). CONCLUSION: Tc-99m DMSA renal scintigraphy had a significant higher efficiency in predicting VUR (a common cause of renal scarring, detected on DMSA) in first febrile urinary tract infection children under the age of 24 months as compared with USG, especially in high-grade reflux.

Microbial cooperation promotes humification to reduce antibiotic resistance genes abundance in food waste composting.

Antibiotic resistance genes (ARGs) fate in a full-scale Food waste (FW) facility was investigated. Results showed that with the changes in ARGs, microbial networks could be naturally divided into two clusters, named as the ARGs increasing group (AI group) and the ARGs decreasing group (AD group). The significant difference between two groups (i.e. stronger microbial competition in the AI group and stronger microbial cooperation in the AD group) implied that the variation in ARGs over time were caused by a switch between competition and cooperation. These results indicated that microbial competition might increase ARGs abundance, while cooperation might reduce it. Meanwhile, structural-equation-model (SEM model) showed that humification indexes (e.g. GI value) was an indicator for characterizing microbial interactions and ARGs. The results of the linear model further confirmed that mature compost (GI values > 92.6 %) could reduce the risk of ARGs.

Interplanting of rice cultivars with high and low Cd accumulation can achieve the goal of "repairing while producing" in Cd-contaminated soil.

Interplanting has been highlighted as a promising, cost-effective, and environmentally friendly solution for the remediation of contaminated soil. In this study, field experiments were conducted to study growth and cadmium (Cd) uptake in monoculture and interplanting systems with rice varieties Changliangyou 772 (C-772) and Changxianggu (Cho-ko-koku). And a pot culture experiment was conducted to investigate the response of the rhizosphere microecology of these two rice varieties. In the interplanting system of the field study, the Cd concentration in the grains of C-772 was significantly reduced (P < 0.05) from 0.30 mg kg-1 to 0.16 mg kg-1 and reached the national food safety standard of China (GB2762-2017, 0.20 mg kg-1), whereas the bioconcentration amount (BCA) per plant of Cd in Cho-ko-koku was significantly increased by 68.18 % (P < 0.05). The land equivalent ratio (LER) and Cd metal removal equivalent ratio (MRER) of the interplanting system were 1.03 and 1.05, illustrating that the interplanting system was superior in terms of yield and Cd elimination. In the pot experiment, the interplanting system significantly (P < 0.05) reduced the iron plaque content on the root surface and organic acids content in the rhizosphere environment of C-772 while markedly increasing those levels in Cho-ko-koku. At ripening stage, the interplanting system significantly decreased the rhizosphere available Cd concentration of C-772 from 0.38 mg kg-1 to 0.22 mg kg-1 (P < 0.05), while significantly increased the rhizosphere available Cd concentration of Cho-ko-koku from 0.27 mg kg-1 to 0.32 mg kg-1 (P < 0.05). Thus, Cd uptake of C-772 and Cho-ko-koku showed apparent differences. Oxalic and tartaric acid were identified as the most crucial factors affecting Cd uptake by C-772 and Cho-ko-koku in the interplanting system, respectively. In summary, this interplanting system is a promising planting pattern that can simultaneously improve land use efficiency and alleviate Cd pollution.