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1.
Klin Med (Mosk) ; 86(6): 58-61, 2008.
Artigo em Russo | MEDLINE | ID: mdl-18720714

RESUMO

Levels of lactoferrin (LF), antithrombin-III (AT-III) and beta2-macroglobulin (MG) in blood of patients with rheumatoid arthritis (RA), reactive arthritis (ReA) and systemic lupus erythematosus (SLE) were examined for assessment their importance in differential diagnostics of the diseases. It was shown that on the average, LF and AT-III levels were increased at RA, while MG level was practically unchanged. At the same time LF level was stable high regardless of RA activity and duration degree, but its concentration was significantly increased also in ReA patients (33% patients). AT-III, on the contrary, depended on process activity and duration, was more specific to RA, than LF, but its sensitivity at RA was not enough high (from 25 to 44% patients with increased levels subject to disease duration and activity). Coefficient LFE/AT-III, obtained by multiplication of these proteins concentration, had the greatest diagnostic value. Its sensitivity at RA is on the average 85%, and specificity at differential diagnostics of RA--80% vs. ReA and 92% vs. SLE. We consider that coefficient LFbetaAT-III can be used as an additional criterion at differential diagnostics at RA early stages, while other specific antibodies cannot be detected yet.


Assuntos
Proteínas de Fase Aguda/metabolismo , Artrite Reumatoide/diagnóstico , Biomarcadores/sangue , Inflamação/sangue , Antitrombina III/metabolismo , Artrite Reumatoide/sangue , Diagnóstico Diferencial , Feminino , Humanos , Técnicas Imunoenzimáticas , Lactoferrina/sangue , Macroglobulinas/metabolismo , Masculino , Pessoa de Meia-Idade , Prognóstico , Proibitinas
2.
Int J Exp Pathol ; 80(2): 105-8, 1999 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-10469265

RESUMO

Rat macroglobulins were determined in blood sera and extracts of tissues of intact rats and rats with Walker carcinoma by rocket immunoelectrophoresis. The serum levels of alpha1-macroglobulin (alpha1MG) alpha2-macroglobulin (alpha2MG) and pregnancy-associated alpha1-glycoprotein (alpha1PAG) were 1.86 +/- 0.07 mg/ml, 0.12 +/- 0. 02 mg/ml and 18.32 +/- 4.07 AU/ml respectively in control rats. Maximum concentrations of alpha1MG were found in heart, lung and spleen and lesser quantities were in liver and thymus, while alpha2MG and alpha1PAG were not found at all in tissue extracts from control rats. Serum alpha2MG and alpha1PAG concentrations increased more than 30-fold in tumour-bearing rats compared to control animals, while alpha1MG serum concentration was little changed. Increases in all three macroglobulins occurred in the tissues of tumour-bearing rats, particularly alpha1PAG. The tissue concentrations of alpha1MG and alpha2MG were similar and the tissue distribution was also similar with highest concentrations in heart and lung. Considerable quantities of the proteins were found in the tumour and part of peritoneum which made contact with the tumour. Changes in the protein concentrations in serum and tissue extracts of tumour-bearing rats suggest that all members of rat macroglobulin family are disturbed during the development of the Walker carcinoma, though only alpha2MG and alpha1PAG were substantially elevated.


Assuntos
Carcinoma 256 de Walker/metabolismo , Macroglobulinas/metabolismo , Proteínas de Neoplasias/metabolismo , Animais , Carcinoma 256 de Walker/sangue , Proteínas de Transporte/metabolismo , Imunoeletroforese , Masculino , Proteínas de Neoplasias/sangue , Proteínas da Gravidez/metabolismo , Ratos , Distribuição Tecidual
3.
Russ J Immunol ; 1(1): 55-60, 1996 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-12687043

RESUMO

Native and modified with ammonium ions alpha(2)-macroglobulin forms exhibit similar abilities to influence proliferative activity and secretion of immunoglobulins in cultures of human blood mononuclear cells in vitro. Effects of modified with ammonium ions alpha(2)-macroglobulin were more significant than those of native alpha(2)-macroglobulin. Revealed properties of native alpha(2)-macroglobulin could be conditioned by a partial modification of its structure with endogenous proteinases or cytokines, some of which were shown to be common affinants for both forms.

4.
Ontogenez ; 26(2): 119-24, 1995.
Artigo em Russo | MEDLINE | ID: mdl-7539904

RESUMO

Glycoproteins similar in composition, structure, and functions were isolated from the blood serum of pregnant women, cows, dogs, and rats. They consist of four identical subunits, which form dimers due to covalent bonds, while dimers form tetramers due to hydrogen bonds. All these proteins are non-specific inhibitors of proteases and bind these enzymes in the same way as alpha-macroglobulins do, by capture in a "trap". Furthermore, they are capable of binding and transporting various cytokines. The results obtained indicate that these proteins belong to the family of macroglobulins.


Assuntos
Proteína Plasmática A Associada à Gravidez/análise , Marcadores de Afinidade , Animais , Bovinos , Fenômenos Químicos , Físico-Química , Cães , Eletroforese em Gel de Poliacrilamida , Feminino , Humanos , Imunoeletroforese , Focalização Isoelétrica , Gravidez , Proteína Plasmática A Associada à Gravidez/química , Proteína Plasmática A Associada à Gravidez/isolamento & purificação , Ratos , Especificidade da Espécie
5.
Int J Exp Pathol ; 75(6): 425-31, 1994 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-7537521

RESUMO

Antiserum against rat alpha 1-macroglobulin (alpha 1MG) was produced in rabbits. Antiserum against rat pregnancy-associated alpha 1-glycoprotein (PAG) was obtained by immunization with a partly purified PAG preparation and absorption of the serum with male rat serum. Acute inflammation was produced in non-pregnant female rats by a single intramuscular injection of turpentine. The concentrations of both macroglobulins in the serum and in tissue extracts were measured by rocket immunoelectrophoresis at various times up to 7 days after injury. Inflammation produced in the rats resulted in moderately elevated serum levels of these proteins soon after injury. At first, alpha 1MG levels in a number of tissues (heart, lung, kidney, spleen, pancreas, uterus and ovary) were depressed markedly; they then stabilized. The elevated serum concentrations of alpha 1MG remained unchanged during inflammation. The store of PAG in the tissues was rapidly depleted and its serum level decreased to a normal value 7 days after injury. Our findings indicate that alpha 1MG plays a more important role in maintenance of the proteinase inhibitory potential in the rat than does PAG.


Assuntos
Reação de Fase Aguda/metabolismo , Proteínas de Transporte/metabolismo , Proteínas da Gravidez/metabolismo , alfa-Macroglobulinas/metabolismo , Reação de Fase Aguda/sangue , Reação de Fase Aguda/induzido quimicamente , Animais , Proteínas de Transporte/sangue , Cromatografia de Afinidade , Feminino , Soros Imunes , Imunoeletroforese Bidimensional , Masculino , Proteínas da Gravidez/sangue , Ratos , Ratos Wistar , Distribuição Tecidual , Terebintina , alfa-Macroglobulinas/isolamento & purificação
6.
Zh Evol Biokhim Fiziol ; 30(4): 505-10, 1994.
Artigo em Russo | MEDLINE | ID: mdl-7863742

RESUMO

Studies have been made on physico-chemical and antigenic properties of albumins from man, mammals, birds, insects, amphibians, worms, fishes and crustaceans. It was found that all the animals contain structurally identical proteins with similar physico-chemical properties. At the same time, the level of antigenic identity of the proteins decreases in the following order: type, genus, family, species.


Assuntos
Antígenos/sangue , Albumina Sérica/química , Albumina Sérica/imunologia , Anfíbios , Animais , Aves , Fenômenos Químicos , Físico-Química , Crustáceos , Peixes , Helmintos , Hemolinfa/química , Hemolinfa/imunologia , Humanos , Insetos , Mamíferos , Peso Molecular
7.
Zh Evol Biokhim Fiziol ; 28(4): 441-6, 1992.
Artigo em Russo | MEDLINE | ID: mdl-1455950

RESUMO

Studies have been made on physicochemical and immunochemical properties of macroglobulins, as well as of associated with pregnancy glycoproteins, from human subjects, mammals, birds, fishes and invertebrates. It was shown that these proteins exhibit similar composition, structure and capacity to bind proteinases inhibiting the latter. Using immunochemical methods, reactions of antigenic identity of these proteins were investigated. A hypothesis of evolutionary formation of macroglobulin family is discussed.


Assuntos
Evolução Biológica , Macroglobulinas/análise , Animais , Western Blotting , Cromatografia em Gel , Feminino , Humanos , Imunodifusão , Imunoeletroforese Bidimensional , Focalização Isoelétrica , Macroglobulinas/isolamento & purificação , Peso Molecular , Gravidez , Especificidade da Espécie
8.
Patol Fiziol Eksp Ter ; (3): 21-2, 1992.
Artigo em Russo | MEDLINE | ID: mdl-1282697

RESUMO

The authors studied the distribution of proteinase inhibitors in inflammation induced by intramuscular turpentine injection. It was found that it reduces the concentration of the studied proteins in the tissues, which is attended by an increase of their plasma levels. The store of pregnancy-associated alpha 1-glycoprotein in the tissues is rapidly depleted, while the concentration of alpha 1-microglobulin reduces to a relatively stable level. The role of the both proteins in the inflammatory process is discussed.


Assuntos
Proteínas de Transporte/metabolismo , Inflamação/metabolismo , Proteínas da Gravidez/metabolismo , alfa-Macroglobulinas/metabolismo , Animais , Feminino , Inflamação/induzido quimicamente , Ratos
9.
Zh Evol Biokhim Fiziol ; 26(3): 289-93, 1990.
Artigo em Russo | MEDLINE | ID: mdl-1699369

RESUMO

It has been demonstrated that antigenic properties of alpha-macroglobulins from 6 species of vertebrate and invertebrate animals are rather similar. All proteins consist of identical subunits which are associated by covalent and noncovalent bonds. All of them are presented by glycoproteins and exhibit the capacity to bind proteinases, as well as the capacity to inhibit the latter with respect to protein and low-molecular synthetic substrates.


Assuntos
Evolução Biológica , alfa-Macroglobulinas/análise , Animais , Carpas , Chironomidae , Cromatografia em Gel , Contraimunoeletroforese , Crustáceos , Eletroforese em Gel de Poliacrilamida , Humanos , Imunodifusão , Peso Molecular , Oligoquetos , Ratos , Especificidade da Espécie
10.
Zh Evol Biokhim Fiziol ; 26(1): 3-8, 1990.
Artigo em Russo | MEDLINE | ID: mdl-1694379

RESUMO

Biochemical and immunochemical properties of human alpha 2-macroglobulin and its analogues from cattle, horse, rabbit, guinea pig, rat, mouse, hen and perch have been investigated. It was found that all analogues have the identical molecular mass and structure, being different in their isoelectric point and carbohydrate composition. It was shown that some antigenic properties of macroglobulins remained constant during evolution, whereas other ones were strictly differentiated at the level of families and species. These data allow to classify macroglobulins as proteins which appeared in vertebrates at early stages of evolution revealing only relatively slight changes during the latter.


Assuntos
alfa-Macroglobulinas/análise , Animais , Bovinos , Galinhas , Cromatografia de Afinidade , Contraimunoeletroforese , Cyprinidae , Proteínas do Ovo/análise , Eletroforese em Gel de Poliacrilamida , Cobaias , Cavalos , Humanos , Imunodifusão , Focalização Isoelétrica , Camundongos , Peso Molecular , Percas , Coelhos , Ratos , Especificidade da Espécie
12.
Zh Evol Biokhim Fiziol ; 21(2): 130-3, 1985.
Artigo em Russo | MEDLINE | ID: mdl-3993277

RESUMO

Using disc-electrophoresis in polyacrylamide gel and immunochemical methods, studies have been made on proteins from the vitreous body of mammals (albino mouse, rat, guinea pig, pig, dog, cat), birds (hen), amphibians (the frog Rana ridibunda) and fish (the perch Perca fluviatilis). It was found that vitreous body proteins in man and animals include both the specific proteins and those of the blood serum. During evolution, specific antigens of the vitreous body attained strict species specificity, although some of them preserved the initial properties.


Assuntos
Proteínas do Olho/análise , Vertebrados/metabolismo , Corpo Vítreo/análise , Animais , Gatos , Bovinos , Galinhas , Contraimunoeletroforese , Cães , Eletroforese em Gel de Poliacrilamida , Feminino , Peixes , Cobaias , Cavalos , Humanos , Camundongos , Coelhos , Rana ridibunda , Ratos , Suínos
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