RESUMO
To address the reality that LGBTQ+ (lesbian, gay, bisexual, transgender, queer, intersex, asexual, and others) students remain more likely to experience harm, harassment, and violence at school as well as miss school due to feeling unsafe and the fact that students identifying as transgender, nonbinary, and gender-nonconforming (TNBGNC) are at even greater risk of bullying, harassment, and significant mental health concerns, Chicago Public Schools' (CPS) Office of Student Health and Wellness (OSHW) created a novel professional development (PD) requirement in 2019, entitled "Supporting Transgender, Nonbinary, and Gender Nonconforming Students." The PD, a recorded webinar encouraging independent time for reflection and planning, takes an intersectional approach and is required of all CPS staff members across the entire district. A pre- and postevaluation of the PD, guided by the Kirkpatrick model, was completed by 19,503 staff members. The findings from this evaluation show that staff members significantly increased their knowledge, showed statistically significant gain in self-reported skills, and articulated key actions they could take toward sustaining an environment that fosters skill implementation and culture change more broadly. Findings reveal that a culture that supports staff members in learning from their mistakes can help to encourage staff members to employ gender-inclusive behaviors such as asking individuals for their pronouns and using gender-neutral pronouns. This districtwide mandatory PD approach shows value in influencing staff members' thinking and behaviors known to be supportive of TNBGNC students and may serve as a model for other school districts looking to build capacity to support TNBGNC students.
RESUMO
The objectives of this study were to activate human colonic intraepithelial lymphocytes at the transcriptional level with HLA-DR+ human colonic epithelial cell line (HT29) in synergy with CD3 monoclonal antibody and to investigate the molecular mechanism for the therapeutic effects of 5-aminosalicylic acid. Lymphocytes were isolated by a mechanical method from resected colon of 22 cases and then cocultured on 10 ng/ml CD3mAb immobilized plates with HT29 which had been induced to express MHC class II molecules by interferon gamma. Flow cytometry analysis suggested that the lymphocyte population had a CD4/CD8 ratio similar to that observed in intact tissue sections and that there was no HT29 contamination of the lymphocytes isolated again from cocultured cells. The activation of intraepithelial lymphocytes showed the gene transcription of interferon gamma and tumor necrosis factor alpha, as measured by means of the reverse-transcriptase polymerase chain reaction, and this activation was antagonized by 5-aminosalicylic acid. Thus, epithelial cells bearing HLA-DR are capable of enhancing CD3-induced activation of human colonic intraepithelial lymphocytes and subject to inhibition by 5-aminosalicylic acid, the active moiety of salicylates used in inflammatory bowel disease.
Assuntos
Ácidos Aminossalicílicos/farmacologia , Citocinas/efeitos dos fármacos , Antígenos HLA-DR/metabolismo , Linfócitos/metabolismo , Actinas/análise , Adulto , Idoso , Idoso de 80 Anos ou mais , Anti-Inflamatórios não Esteroides/farmacologia , Sequência de Bases , Técnicas de Cocultura , Colite Ulcerativa/imunologia , Colo/citologia , Doença de Crohn/imunologia , Citocinas/genética , Epitélio/fisiologia , Feminino , Regulação da Expressão Gênica , Antígenos HLA-DR/efeitos dos fármacos , Humanos , Interferon gama/análise , Interferon gama/farmacologia , Linfócitos/efeitos dos fármacos , Masculino , Mesalamina , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Células Tumorais Cultivadas , Fator de Necrose Tumoral alfa/análiseRESUMO
Human colonic intraepithelial lymphocytes from control subjects down-regulate the proliferative responses of primed allogeneic peripheral blood mononuclear cells on rechallenge with antigens or phytohaemagglutinin (PHA). In contrast, human colonic intraepithelial lymphocytes from patients with inflammatory bowel disease fail to down-regulate the proliferative responses of primed allogeneic peripheral blood mononuclear cells on rechallenge with antigens. These findings may be important in the development and maintenance of the mucosal immunological activation of inflammatory bowel disease.
Assuntos
Antígenos de Bactérias/imunologia , Colo/imunologia , Doenças Inflamatórias Intestinais/imunologia , Ativação Linfocitária/imunologia , Linfócitos T/imunologia , Toxoide Tetânico/imunologia , Idoso , Idoso de 80 Anos ou mais , Células Cultivadas , Colite Ulcerativa/imunologia , Colo/patologia , Doença de Crohn/imunologia , Regulação para Baixo/imunologia , Epitélio/imunologia , Feminino , Humanos , Mucosa Intestinal/imunologia , Masculino , Pessoa de Meia-Idade , Fito-Hemaglutininas/imunologia , Linfócitos T Reguladores/imunologiaRESUMO
Human colonic intraepithelial lymphocytes have been shown to suppress the proliferation of autologous lamina propria lymphocytes and allogeneic peripheral blood mononuclear cells. This study has shown that, in vitro, intraepithelial lymphocytes suppress IgA and total immunoglobulin synthesis (but not IgG or IgM production) by autologous peripheral blood and lamina propria lymphocytes. This down regulation of IgA production is mediated by a soluble factor secreted by the intraepithelial lymphocytes. There is no difference in immunoglobulin down regulation by intraepithelial lymphocytes of control subjects and patients with inflammatory bowel disease.
Assuntos
Colo/imunologia , Tolerância Imunológica/imunologia , Imunoglobulinas/biossíntese , Doenças Inflamatórias Intestinais/imunologia , Linfócitos T/imunologia , Adolescente , Adulto , Idoso , Divisão Celular/imunologia , Células Cultivadas , Regulação para Baixo , Epitélio/imunologia , Feminino , Humanos , Imunoglobulina A/biossíntese , Imunoglobulina G/biossíntese , Imunoglobulina M/biossíntese , Masculino , Pessoa de Meia-Idade , Linfócitos T/metabolismoRESUMO
Emotional intelligence involves the accurate appraisal and expression of emotions in oneself and others and the regulation of emotion in a way that enhances living. One aspect of emotional intelligence is the ability to recognize the consensually agreed upon emotional qualities of objects in the environment. One hundred thirty-nine adults viewed 18 reproductions of faces, color swatches, and abstract designs and rated the emotional content of these visual stimuli. Three scores were extracted, including consensual accuracy, amount, and range of emotion perceived. These scores were compared with other aspects of emotional intelligence such as empathy and related to constructs such as alexithymia and neuroticism. A general ability to perceive consensual emotional content in visual stimuli was found, and it was most strongly associated with the ability to respond empathically to others.
Assuntos
Afeto , Emoções , Percepção de Forma , Imaginação , Inteligência , Adolescente , Adulto , Percepção de Cores , Expressão Facial , Fantasia , Feminino , Humanos , Masculino , Pessoa de Meia-IdadeAssuntos
Infecções Tumorais por Vírus/cirurgia , Neoplasias do Colo do Útero/cirurgia , Biópsia , Colo do Útero/patologia , Feminino , Seguimentos , Humanos , Recidiva Local de Neoplasia/cirurgia , Estadiamento de Neoplasias , Papillomaviridae/ultraestrutura , Infecções Tumorais por Vírus/patologia , Neoplasias do Colo do Útero/patologiaRESUMO
An extensive taxonomic investigation identified strain SK&F-AAJ-271 as a new species, designated Actinomadura parvosata. Fermentations of this organism produce a complex of acidic, lipophilic glycopeptide antibiotics, the parvodicins. Structures for seven of the isolated components were derived from a combination of mass spectral, high-field NMR and chemical techniques. The O-acetyl functionality present in two of the isolated components is a structural feature unique among the known members of this class of antibiotics. The parvodicins are active in vitro against a range of Gram-positive bacteria. The most active parvodicin, C1, produces high serum levels in vivo and has the potential for a long duration of action.