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1.
Int J Dent Hyg ; 22(1): 187-193, 2024 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-37722074

RESUMO

OBJECTIVE: This study aimed to assess the knowledge of monkeypox infection among dental hygiene professionals and students in Saudi Arabia. METHODS: A cross-sectional study was conducted among a convenience sample of dental hygiene professionals (n = 259). The questionnaire was developed based on previous literature, and descriptive analysis and a Chi-square test were performed. RESULTS: A total of 159 dental hygienists responded to the questionnaire. Results indicated that only 1.7% had good knowledge, 7.7% had moderate knowledge and 90.6% had low knowledge of the monkeypox outbreak. The mean knowledge scores varied among dental hygiene students, practitioners and faculty members. Significant group differences were observed for some questions (p < 0.05). CONCLUSION: Our study concludes that dental hygienists had moderate to low level of knowledge of the monkeypox infection and its implications for oral health and patient care, indicating a need for more education on the subject.


Assuntos
Higienistas Dentários , Mpox , Humanos , Estudos Transversais , Arábia Saudita/epidemiologia , Estudantes de Odontologia
2.
PLoS One ; 17(7): e0271664, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35862403

RESUMO

OBJECTIVES: Multiple studies show orthopedic health problems for medical staff due to wearing radiation protection aprons. The aim of this study was to evaluate the weight pressure on the shoulder as a marker of physical strain caused by different radiation-protection devices. METHODS: For the weight pressure measurement, a pressure sensor (OMD-30-SE-100N, OptoForce, Budapest, Hungary) placed on the left and right shoulder was used. Wearing different radiation protection systems the force measurement system was used to quantify the weight pressure. Measurements were acquired in still standing position and during various movements. RESULTS: A mean significant decreasing weight pressure on the shoulder between 74% and 84% (p<0.001) was measured, when the free-hanging radiation protection system was used in comparison to one-piece and two-piece radiation protection aprons and coats. Using two-piece radiation protection aprons, the weight pressure was significantly lower than that of one-piece radiation protection coats. If a belt was used for the one-piece radiation protection coat, the weight pressure on the shoulder was reduced by 32.5% (p = 0.003). For a two-piece radiation protection apron and a one-piece radiation protection coat (with and without belt) a significant different weight pressure distribution between the right and left shoulder could be measured. CONCLUSIONS: The free-hanging radiation protection system showed a significant lower weight pressure in comparison to the other radiation protection devices. Apart from this, use of a two-piece radiation protection apron or addition of a belt to a radiation protection coat proved to be further effective options to reduce weight pressure.


Assuntos
Exposição Ocupacional , Proteção Radiológica , Humanos , Hungria , Corpo Clínico , Exposição Ocupacional/análise , Exposição Ocupacional/prevenção & controle , Roupa de Proteção , Doses de Radiação , Radiologia Intervencionista
3.
J Vasc Interv Radiol ; 33(2): 104-112, 2022 02.
Artigo em Inglês | MEDLINE | ID: mdl-34653607

RESUMO

PURPOSE: To evaluate the potential benefits of digital variance angiography (DVA) in selective lower limb angiography and to compare the performance of 2 DVA algorithms (conventional DVA1 and the recently developed DVA2) to that of digital subtraction angiography (DSA). MATERIALS AND METHODS: From November 2019 to May 2020, 112 iodinated contrast media (ICM) and 40 carbon dioxide (CO2) angiograms were collected from 15 and 13 peripheral artery disease patients, respectively. The DVA files were retrospectively generated from the same unsubtracted source file as DSA. The objectively calculated contrast-to-noise ratio (CNR) and the subjective visual image quality of DSA, DVA1, and DVA2 images were statistically compared using the Wilcoxon signed-rank test. The images were evaluated by 6 radiologists (R.P.T., S.V., A.M.K., S.S.A., O.E., and J.S.) from 2 centers using a 5-grade Likert scale. RESULTS: Both DVA algorithms produced similar increase (at least 2-fold) in CNR values (P < .001) and significantly higher image quality scores than DSA, independent of the contrast agent used. The overall scores with ICM were 3.61 ± 0.05 for DSA, 4.30 ± 0.04 for DVA1, and 4.33 ± 0.04 for DVA2 (each P < .001 vs DSA). The scores for CO2 were 3.10 ± 0.14 for DSA, 3.63 ± 0.13 for DVA1 (P < .001 vs DSA), and 3.38 ± 0.13 for DVA2 (P < .05 vs DSA). CONCLUSIONS: DVA provides higher CNR and significantly better image quality in selective lower limb interventions irrespective of the contrast agent used. Between DVA algorithms, DVA1 is preferred because of its identical or better image quality than DVA2. DVA can potentially help the interventional decision process and its quality reserve might allow dose management (radiation/ICM reduction) in the future.


Assuntos
Extremidade Inferior , Doença Arterial Periférica , Angiografia Digital/métodos , Meios de Contraste , Humanos , Extremidade Inferior/irrigação sanguínea , Doença Arterial Periférica/diagnóstico por imagem , Estudos Retrospectivos
4.
Health Phys ; 120(6): 641-647, 2021 06 01.
Artigo em Inglês | MEDLINE | ID: mdl-33879646

RESUMO

ABSTRACT: The maximum annual radiation ocular dose limit for medical staff has been reduced to 20 mSv in the current European directive 2013/59/Euratom. This multi-centric study aims at reporting the protected and unprotected eye lens doses in different fluoroscopically guided interventions and to evaluate any other factors that could influence the ocular dose. From July 2018 to July 2019, ocular radiation doses of six interventionists of four departments during complex interventions were recorded with a thermoluminescent dosimeter in front of and behind radiation protection glasses to measure the protected and unprotected doses. The position of personnel, intervention type, fluoroscopy time, total body dose and use of pre-installed protection devices like lead acrylic shields were also systematically recorded. Linear regression analysis was used to estimate the doses at 2 y and 5 y. The annual unprotected/protected ocular doses of six interventionists were 67/21, 32.7/3.3, 27.4/5.1, 7/0, 21.8/2.2, and 0/0 mSv, respectively. The unprotected dose crossed the 20-mSv annual limits for four interventionists and protected dose for one less experienced interventionist. The estimated 5-y protected ocular dose of this interventionist was 101.318 mSv (95%CI 96.066-106.57), also crossing the 5-y limit. The use of a lead acrylic shield was observed to have a significant effect in reducing ocular doses. The annual unprotected and protected ocular doses for interventionists dealing with complex interventions could cross the present permitted yearly limit. The measurement of significant protected ocular dose behind the radiation protection glasses emphasizes the additional indispensable role of pre-installed radiation protection devices and training in reducing radiation doses for complex procedures.


Assuntos
Cristalino , Exposição Ocupacional , Exposição à Radiação , Proteção Radiológica , Dispositivos de Proteção dos Olhos , Humanos , Cristalino/efeitos da radiação , Exposição Ocupacional/análise , Exposição Ocupacional/prevenção & controle , Doses de Radiação , Exposição à Radiação/análise , Exposição à Radiação/prevenção & controle , Proteção Radiológica/métodos , Raios X
5.
Open Med (Wars) ; 16(1): 87-94, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33392391

RESUMO

Foci of splenic tissue separated from the spleen can occur as a congenital anomaly. Isolated nodules of splenic tissue are called accessory spleens or spleniculli. However, nodules of splenic tissue can merge with other organs during embryonic development, in which case we speak of spleno-visceral fusions: most often, they merge with the tail of the pancreas (thus forming spleno-pancreatic fusion or an intrapancreatic accessory spleen), with the reproductive gland (i.e., spleno-gonadal fusion), or with the kidney (i.e., spleno-renal fusion). Our case report describes the fusion of heterotopic splenic tissue with the right adrenal gland, which was misinterpreted as a metastasis of a renal cell carcinoma. To the best of our knowledge, this is the first reported case of spleno-adrenal fusion. Spleno-visceral fusions usually represent asymptomatic conditions; their main clinical significance lies in the confusion they cause and its misinterpretation as tumors of other organs. We believe that the cause of retroperitoneal spleno-visceral fusions is the anomalous migration of splenic cells along the dorsal mesentery to the urogenital ridge, together with primitive germ cells, at the end of the fifth week and during the sixth week of embryonic age. This theory explains the possible origin of spleno-visceral fusions, their different frequency of occurrence, and the predominance of findings on the left side.

6.
Medicine (Baltimore) ; 100(2): e24254, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-33466210

RESUMO

ABSTRACT: Carbon dioxide (CO2) gas is an established alternative to iodine contrast during angiography in patients with risk of postcontrast acute kidney injury and in those with history of iodine contrast allergy. Different CO2 delivery systems during angiography are reported in literature, with automated delivery system being the latest. The aim of this study is to evaluate the safety, efficacy, and learning curve of an automated CO2 injection system with controlled pressures in peripheral arterial interventions and also to study the patients' tolerance to the system.From January 2018 to October 2019 peripheral arterial interventions were performed in 40 patients (median age-78 years, interquartile range: 69-84 years) using an automated CO2 injection system with customized protocols, with conventional iodine contrast agent used only as a bailout option. The pain and tolerance during the CO2 angiography were evaluated with a visual analog scale at the end of each procedure. The amount of CO2, iodine contrast used, and radiation dose area product for the interventions were also systematically recorded for all procedures. These values were statistically compared in 2 groups, viz first 20 patients where a learning curve was expected vs the rest 20 patients.All procedures were successfully completed without complications. All patients tolerated the CO2 angiography with a median total pain score of 3 (interquartile range: 3-4), with no statistical difference between the groups (P = .529). The 2 groups were statistically comparable in terms of comorbidities and the type of procedures performed (P = .807). The amount of iodine contrast agent used (24.60 ±â€Š6.44 ml vs 32.70 ±â€Š8.70 ml, P = .006) and the radiation dose area product associated were significantly lower in the second group (2160.74 ±â€Š1181.52 µGym2 vs 1531.62 ±â€Š536.47 µGym2, P = .043).Automated CO2 angiography is technically feasible and safe for peripheral arterial interventions and is well tolerated by the patients. With the interventionalist becoming familiar with the technique, better diagnostic accuracy could be obtained using lower volumes of conventional iodine contrast agents and reduction of the radiation dose involved.


Assuntos
Angiografia/métodos , Dióxido de Carbono/administração & dosagem , Procedimentos Endovasculares/métodos , Doença Arterial Periférica/cirurgia , Idoso , Idoso de 80 Anos ou mais , Meios de Contraste , Estudos de Viabilidade , Feminino , Humanos , Compostos de Iodo , Masculino , Estudos Retrospectivos , Resultado do Tratamento
7.
Artigo em Inglês | MEDLINE | ID: mdl-32116311

RESUMO

AIM: The aim of this retrospective study was to analyse the preoperative ultrasound findings in patients with minimal or almost no morphological changes of axillary lymph nodes (LN) and to correlate these findings with the results of sentinel node (SN) biopsy. MATERIALS AND METHODS: Between January 2014 and September 2018, 289 female patients with newly diagnosed breast cancer and negative preoperative axillary staging were examined with preoperative ultrasound evaluation of axillary LNs. Patients with no evidence of LN metastases underwent primary surgical treatment with SN biopsy. Negative predictive value (NPV) of preoperative ultrasound was evaluated and the histopathological findings in positive SN biopsies were correlated with tumour type and preoperative ultrasound LN imaging. RESULTS: Of 289 patients with negative preoperative axillary staging who had primary surgical treatment, 268 patients had negative SN biopsy while SN metastases were detected in 21 patients. Of patients with positive SN biopsies, 2 patients had negative core biopsy of axillary LN before surgery. The preoperative ultrasound examination was negative in the remaining 19 patients with SN metastases. CONCLUSIONS: Preoperative ultrasonography is very accurate in the detecting of axillary LN metastases. Patients with primary tumour size ≥ 1 cm, with grade ≥ 2 no special type carcinomas (NST - no special type, also known as invasive ductal carcinoma) or multicentric lobular invasive cancer should undergo a more thorough ultrasound evaluation.


Assuntos
Neoplasias da Mama/patologia , Biópsia de Linfonodo Sentinela , Axila , Neoplasias da Mama/cirurgia , Feminino , Humanos , Estadiamento de Neoplasias , Período Pré-Operatório , Estudos Retrospectivos
8.
Artigo em Inglês | MEDLINE | ID: mdl-32116312

RESUMO

AIMS: The coverage / occlusion of internal iliac artery (IIA) during endovascular treatment of aorto-iliac aneurysms (AIA) can be associated with risk of ischemic complications. To reduce these complications, unilateral or bilateral iliac branch device implantation (IBDI) has been reported. This study aims at evaluating the efficacy of simultaneous unilateral IBDI in the treatment of AIAs and comparing our results with literature. MATERIALS AND METHODS: From March 2010 to December 2019, 27 patients (25 men, 2 women, range 54-84 years) were treated for aorto-iliac/isolated common iliac aneurysms with simultaneous unilateral revascularization of IIA and surgical / endovascular occlusion of contralateral IIA. 27 iliac-branched devices were implanted in 27 patients. The results including ischemic complications were evaluated and compared with literature. RESULTS: The technical success was 100% with no perioperative mortality and morbidity of 3.7%. Primary internal iliac branch patency at a median follow-up of 52 months (range 1-118 months) was 96.42%. Secondary endoleak was observed in 6 patients (Type 1a [1], Type 1b [1], Type II [4]) and inflammatory complication in 1 patient. The incidence of buttock claudication one year after the procedure was 11.1%. Except for buttock claudication no other ischemic complications occurred. CONCLUSION: Unilateral flow preservation in the IIA territory using IBDI is associated with a lesser, but a certain risk of ischemic complications. Bilateral IBDI with bilateral flow preservation of IIAs increases the complexity, procedure -/ fluoroscopy times, contrast agent volume and cost, however, may further reduce these ischemic complications.


Assuntos
Implante de Prótese Vascular , Procedimentos Endovasculares , Aneurisma Ilíaco , Prótese Vascular , Feminino , Humanos , Aneurisma Ilíaco/diagnóstico por imagem , Aneurisma Ilíaco/cirurgia , Artéria Ilíaca/cirurgia , Claudicação Intermitente , Isquemia/etiologia , Isquemia/cirurgia , Masculino , Desenho de Prótese , Estudos Retrospectivos , Stents , Resultado do Tratamento
9.
Cardiovasc Intervent Radiol ; 43(12): 1798-1807, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33000316

RESUMO

Twenty-five years ago, optimal medical management was the mainstay of treatment in acute type B aortic dissection (TBAD) and intramural haematoma (IMH), with surgery being reserved for cases with rupture or critical branch vessel ischaemia. Less invasive endoluminal management of TBAD and IMH has developed rapidly over the past two and a half decades, thus changing the treatment algorithm in these patients. Today the focus has shifted to primary management with a combination of endoluminal intervention and optimal medical treatment. The purpose of this article is to describe the various interventional techniques, discuss the indications for intervention, and present the results in the current literature regarding clinical (rupture, branch vessel ischaemia) as well as morphological response (aortic remodelling), complications, and morbidity/mortality associated with endoluminal intervention.


Assuntos
Aneurisma Aórtico/cirurgia , Dissecção Aórtica/cirurgia , Implante de Prótese Vascular , Procedimentos Endovasculares , Hematoma/cirurgia , Stents , Aneurisma Aórtico/diagnóstico por imagem , Angiografia por Tomografia Computadorizada , Procedimentos Endovasculares/efeitos adversos , Hematoma/diagnóstico por imagem , Humanos , Isquemia/cirurgia
10.
Braz J Cardiovasc Surg ; 35(4): 411-419, 2020 08 01.
Artigo em Inglês | MEDLINE | ID: mdl-32864918

RESUMO

INTRODUCTION: People with aortic/prosthetic valve endocarditis are a high-risk cohort of patients who present a challenge for all medically involved disciplines and who can be treated by various surgical techniques. METHODS: We analyzed the results of treatment of root endocarditis with Medtronic Freestyle® in full-root technique over 19 years (1999-2018) and compared them against treatment with other tissue valves. Comparison was made with propensity score matching, using the nearest neighbor method. Various tests were performed as suited for adequate analyses. RESULTS: Fifty-four patients in the Medtronic Freestyle group (FS group) were matched against 54 complex root endocarditis patients treated with other tissue valves (Tissue group). Hospital mortality was 9/54 (16.7%) in the FS group vs. 14/54 (25.6%) in the Tissue group (P=0.24). Cox regression performed for early results demonstrated coronary heart disease (P=0.004, odds ratio 2.3), among others, influencing early mortality. Recurrent infection was low (1.8% for FS and Tissue patients) and freedom from reoperation was 97.2% at a total of 367 patient-years of follow-up (median of 2.7 years). CONCLUSION: The stentless xenograft is a viable alternative for treatment of valve/root/prosthetic endocarditis, demonstrating a low rate of reinfection. The design of the bioroot allows for complex reconstructive procedures at the outflow tract and the annular level with at an acceptable operative risk. Endocarditis patients can be treated excluding infective tissue from the bloodstream, possibly with benefits, concerning bacteremia and recurrent infection. Furthermore, the use of the stentless bioroot offers varying treatment options in case of future valve degeneration.


Assuntos
Bioprótese , Endocardite Bacteriana , Implante de Prótese de Valva Cardíaca , Próteses Valvulares Cardíacas , Idoso , Valva Aórtica/cirurgia , Bioprótese/efeitos adversos , Endocardite Bacteriana/cirurgia , Feminino , Próteses Valvulares Cardíacas/efeitos adversos , Implante de Prótese de Valva Cardíaca/efeitos adversos , Humanos , Masculino , Pessoa de Meia-Idade , Pontuação de Propensão , Desenho de Prótese , Reoperação , Resultado do Tratamento
11.
Rev. bras. cir. cardiovasc ; 35(4): 411-419, July-Aug. 2020. tab, graf
Artigo em Inglês | LILACS, Sec. Est. Saúde SP | ID: biblio-1137311

RESUMO

Abstract Introduction: People with aortic/prosthetic valve endocarditis are a high-risk cohort of patients who present a challenge for all medically involved disciplines and who can be treated by various surgical techniques. Methods: We analyzed the results of treatment of root endocarditis with Medtronic Freestyle® in full-root technique over 19 years (1999-2018) and compared them against treatment with other tissue valves. Comparison was made with propensity score matching, using the nearest neighbor method. Various tests were performed as suited for adequate analyses. Results: Fifty-four patients in the Medtronic Freestyle group (FS group) were matched against 54 complex root endocarditis patients treated with other tissue valves (Tissue group). Hospital mortality was 9/54 (16.7%) in the FS group vs. 14/54 (25.6%) in the Tissue group (P=0.24). Cox regression performed for early results demonstrated coronary heart disease (P=0.004, odds ratio 2.3), among others, influencing early mortality. Recurrent infection was low (1.8% for FS and Tissue patients) and freedom from reoperation was 97.2% at a total of 367 patient-years of follow-up (median of 2.7 years). Conclusion: The stentless xenograft is a viable alternative for treatment of valve/root/prosthetic endocarditis, demonstrating a low rate of reinfection. The design of the bioroot allows for complex reconstructive procedures at the outflow tract and the annular level with at an acceptable operative risk. Endocarditis patients can be treated excluding infective tissue from the bloodstream, possibly with benefits, concerning bacteremia and recurrent infection. Furthermore, the use of the stentless bioroot offers varying treatment options in case of future valve degeneration.


Assuntos
Humanos , Masculino , Feminino , Pessoa de Meia-Idade , Idoso , Bioprótese/efeitos adversos , Próteses Valvulares Cardíacas/efeitos adversos , Implante de Prótese de Valva Cardíaca/efeitos adversos , Endocardite Bacteriana/cirurgia , Valva Aórtica/cirurgia , Desenho de Prótese , Reoperação , Resultado do Tratamento , Pontuação de Propensão
12.
Sci Rep ; 9(1): 4371, 2019 03 13.
Artigo em Inglês | MEDLINE | ID: mdl-30867492

RESUMO

Our aim was to analyse the hospital outcome for the worldwide largest series of stentless bioroot xenografts (Medtronic Freestyle) as full root replacement in a single centre over a period of 18 years. Retrospective data analysis was performed for the entire cohort of patients undergoing aortic root surgery with the Medtronic Freestyle valve prosthesis. Logistic regression analysis was performed to analyse predictors of in-hospital mortality. 971 patients underwent aortic full root replacement with the Medtronic Freestyle valve in the period from 1999-2017, with an average age of 68.8 ± 10.3y and gender distribution of 608:363 (male:female). Concomitant surgery was performed in 693 patients (71.4%). In-hospital all-comers mortality was 9.8% (95 patients), with the respective highest risk profiles including dissections (6.4%), endocarditis (5.6%) and re-do procedures (12.5%). In-hospital mortality for elective patients was 7.6% while isolated aortic root replacement demonstrated a mortality of 3.6%. Logistic regression analysis demonstrated age (OR 1.05, p = 0.005), dissection (OR 5.78, p < 0.001) and concomitant bypass surgery (OR 2.68, p < 0.001) as preoperative risk factors for the entire cohort. Postoperative analysis demonstrated myocardial infarction (OR 48.6, p < 0.001) and acute kidney injury (OR 20.2, p < 0.001) to be independent risk factors influencing mortality. This analysis presents a work-through of all patients with stentless bioroot treatment without positive selection in a high-volume clinical center with the largest experience world-wide for this form of complex surgery. Isolated aortic root replacement could be performed at acceptable operative risk for this technically-challenging procedure.


Assuntos
Aorta/cirurgia , Próteses Valvulares Cardíacas , Xenoenxertos , Idoso , Anticoagulantes/farmacologia , Valva Aórtica/cirurgia , Comorbidade , Feminino , Doenças das Valvas Cardíacas/cirurgia , Próteses Valvulares Cardíacas/efeitos adversos , Humanos , Masculino , Pessoa de Meia-Idade , Razão de Chances , Estudos Retrospectivos , Resultado do Tratamento
13.
Rofo ; 191(6): 512-521, 2019 Jun.
Artigo em Inglês, Alemão | MEDLINE | ID: mdl-30703826

RESUMO

BACKGROUND: The increasing number of minimally invasive fluoroscopy-guided interventions is likely to result in higher radiation exposure for interventional radiologists and medical staff. Not only the number of procedures but also the complexity of these procedures and therefore the exposure time as well are growing. There are various radiation protection means for protecting medical staff against scatter radiation. This article will provide an overview of the different protection devices, their efficacy in terms of radiation protection and the corresponding dosimetry. METHOD: The following key words were used to search the literature: radiation protection, eye lens dose, radiation exposure in interventional radiology, cataract, cancer risk, dosimetry in interventional radiology, radiation dosimetry. RESULTS AND CONCLUSION: Optimal radiation protection always requires a combination of different radiation protection devices. Radiation protection and monitoring of the head and neck, especially of the eye lenses, is not yet sufficiently accepted and further development is needed in this field. To reduce the risk of cataract, new protection glasses with an integrated dosimeter are to be introduced in clinical routine practice. KEY POINTS: · A combination of personal radiation protection devices and optimized dosimetry improves the safety of medical staff.. CITATION FORMAT: · König AM, Etzel R, Thomas RP et al. Personal Radiation Protection and Corresponding Dosimetry in Interventional Radiology: An Overview and Future Developments. Fortschr Röntgenstr 2019; 191: 512 - 521.


Assuntos
Lesões por Radiação/prevenção & controle , Proteção Radiológica/métodos , Radiologia Intervencionista , Radiometria/métodos , Fluoroscopia/tendências , Previsões , Alemanha , Humanos , Lesões por Radiação/etiologia , Radiologia Intervencionista/tendências , Radiometria/tendências , Espalhamento de Radiação
14.
Methods Mol Biol ; 1855: 491-494, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30426443

RESUMO

We describe here an ultrafast method for electrophoresing proteins on SDS-PAGE. Previously we reported a method to complete SDS-PAGE and immunoblotting in an hour, including electrophoresing proteins at 70°C in 10 min. Here we show that we can electrophorese molecular weight standards and bovine serum albumin on a 4-20% gradient gel in well under 10 min using heated (44 °C) Laemmli running buffer and high voltage.


Assuntos
Eletroforese em Gel de Poliacrilamida/métodos , Proteínas/análise , Animais , Bovinos , Eletroforese em Gel de Poliacrilamida/economia , Eletroforese em Gel de Poliacrilamida/instrumentação , Calefação , Peso Molecular , Proteínas/isolamento & purificação , Soroalbumina Bovina/análise , Soroalbumina Bovina/isolamento & purificação , Dodecilsulfato de Sódio/química , Fatores de Tempo , Trometamina/química
15.
Cardiovasc Intervent Radiol ; 42(2): 313-316, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30382298

RESUMO

Internal iliac artery aneurysms (IIAAs), although rare, are associated with a significant risk of mortality, if they rupture. Endovascular approach with exclusion of the aneurysm sac from antegrade and retrograde perfusion is proved to be a feasible treatment option. However, this option is not always technically possible with a preexisting endovascular aneurysm repair (EVAR) or surgical aortoiliac reconstruction with ligation of internal iliac artery origin. We report another safe treatment option of an enlarging IIAA associated with a type II endoleak after EVAR and a standard endovascular treatment was not possible. The access to the aneurysm sac was achieved retrograde via percutaneous access to the superior gluteal artery under fluoroscopy followed by treatment with embolization of the inflow and outflow vessels.


Assuntos
Aneurisma da Aorta Abdominal/cirurgia , Embolização Terapêutica/métodos , Endoleak/complicações , Procedimentos Endovasculares/métodos , Aneurisma Ilíaco/complicações , Aneurisma Ilíaco/terapia , Idoso de 80 Anos ou mais , Aorta Abdominal/diagnóstico por imagem , Aorta Abdominal/cirurgia , Aneurisma da Aorta Abdominal/diagnóstico por imagem , Nádegas/irrigação sanguínea , Nádegas/diagnóstico por imagem , Endoleak/diagnóstico por imagem , Endoleak/terapia , Fluoroscopia , Humanos , Aneurisma Ilíaco/diagnóstico por imagem , Artéria Ilíaca/diagnóstico por imagem , Masculino , Radiografia Intervencionista/métodos , Tomografia Computadorizada por Raios X
16.
Methods Mol Biol ; 1853: 237-246, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30097949

RESUMO

Curcumin, the principal curcuminoid in the food spice turmeric, is insoluble in water at room temperature. We have previously solubilized curcumin in water with the application of heat (100 °C) and found that this solubilized curcumin could be used as a gel protein stain. However, heat solubilization in water solubilized only a small fraction of the curcuminoid (1.5%), making it relatively expensive to stain one gel ($1.5-2 per gel). Heat/pressure solubilization (121 °C/15 psi) helps increase the solubility of curcumin in water to only 5%. Therefore, we ventured into increasing curcumin solubility further by using detergents (Tween 20, Tween 80, or Triton X-100) combined with heat/pressure treatment. Curcumin solubilized with Tween 20, Tween 80 or Triton X-100 stained proteins efficiently as before. We could dramatically increase the solubility of curcumin by solubilizing in detergents such that we had to dilute the solubilized curcumin 1:100 with water to stain proteins. However, we had to use the soluble curcumin (extracted with heat/pressure alone) straight without dilution. Thus, the amount of curcumin solubilized with detergents was at least 100-fold, with 0.5% Tween 80 being the most efficient. Staining can be carried out in 30 min, and sensitivity of staining is similar or better than that obtained with Coomassie Brilliant Blue (CBB). It is unnecessary to destain the gel, and excess curcumin can be discarded into the sink, unlike CBB. Curcumin solubilized in Tween 20 has been found to be stable for a period of over 4 years. Therefore, curcumin solubilized in Tween 80 or Triton X-100 can serve as an efficient nontoxic and environment-friendly protein stain. Tween 80 has been used in cough syrups to make a colloidal suspension, and also in foods. Solubilizing curcumin with Tween 80 would be a useful alternative to increase curcumin bioavailability in clinical studies.


Assuntos
Curcumina , Detergentes , Temperatura Alta , Pressão , Proteínas , Animais , Curcumina/química , Detergentes/química , Eletroforese em Gel de Poliacrilamida , Octoxinol , Polissorbatos , Estabilidade Proteica , Proteínas/análise , Proteínas/química , Corantes de Rosanilina , Solubilidade , Coloração e Rotulagem/métodos
17.
Cardiovasc Intervent Radiol ; 41(9): 1318-1323, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-29948006

RESUMO

PURPOSE: To evaluate the efficacy of endovascular repair of non-traumatic rupture of the descending thoracic aorta. MATERIALS AND METHODS: From January 2011 to August 2017, 22 consecutive patients (16 men, mean age 73.7 years, range 63-92 years) with non-traumatic rupture of the descending thoracic aorta were treated endovascularly. Thirty-day mortality as well as procedure-related morbidity including stroke, renal, pulmonary and spinal complications were retrospectively assessed. Median follow-up was 29 months (range 6-65 months). RESULTS: Nine patients had type B dissections, and 13 had atherosclerotic aortic aneurysms. The technical success was 100%; 86.3% of patients (19/22) were treated percutaneously. Thirteen patients (59%) received a single endograft, 8 (36%) received 2 endografts, and 1 received 3 endografts. The endograft diameters ranged between 28 and 46 mm. Two dissection patients received additional distal bare metal true lumen stents. Eleven patients required additional drainage of thoracic hematoma. Three primary percutaneous sites (15.7%) required surgical revision. No spinal cord ischemic or renal complications were observed. Stroke was observed in 2 patients (9%), both with full coverage of left subclavian artery origin. Four patients (18%) required tracheostomy with prolonged respiratory support (range 10-15 days). The median intensive care unit stay was 5 days (range 1-27 days). The 30-day mortality was 18.1% (4/22). CONCLUSION: Endoluminal repair of non-traumatic rupture of the descending thoracic aorta can be performed percutaneously. It showed a 30-day mortality of 18.1%, and no spinal cord injury or renal complications were observed in surviving patients at follow-up. LEVEL OF EVIDENCE: Level 4, case series.


Assuntos
Aorta Torácica/cirurgia , Ruptura Aórtica/cirurgia , Implante de Prótese Vascular , Procedimentos Endovasculares/métodos , Idoso , Idoso de 80 Anos ou mais , Aorta Torácica/diagnóstico por imagem , Ruptura Aórtica/diagnóstico por imagem , Prótese Vascular , Feminino , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Ruptura Espontânea , Stents , Fatores de Tempo , Tomografia Computadorizada por Raios X , Resultado do Tratamento
18.
J Cardiothorac Surg ; 13(1): 36, 2018 May 02.
Artigo em Inglês | MEDLINE | ID: mdl-29720205

RESUMO

BACKGROUND: Type A Dissection in pregnancy is a devastating medical condition with 2 lives at stake and unclear strategy at early gestational stages. We describe a successful outcome, clearly dependent on the coordination of all involved disciplines. CASE PRESENTATION: This case history describes a 28 year old female with a 24th week pregnancy gravida 2 para 0 with a DeBakey Type I aortic dissection, diagnosed via ultrasound. Surgery was perfomed on the day of diagnosis. After conferral with the mother, caesarean section was performed and a 690 g fetus could be delivered and was immediately transferred to the neonatal unit. Subsequent aortic repair was performed after hysterectomy, with replacement of the ascending aorta and hemiarch treatment. Intraoperatively no entry in the ascending aorta or transverse arch could be demonstrated, so that a retrograde Type A with entry distal to the left subclavian had to be postulated. We decided to perform subsequent computer tomography, demonstrating multiple entry sites in the descending aorta distal to the left subclavian artery. Successful endovascular treatment could be performed with a Medtronic Valiant Stent via a transfemoral approach. The further hospital stay was uneventful and the patient could be discharged on the 18th postoperative day. The baby demonstrated fighter qualities and could be discharged home after a 3 month hospital stay to be reunited with his mother. CONCLUSION: Prompt diagnosis, precise coordination between all involved subspecialties and ultimately, as in this case, definitive treatment in consensus with operative and interventional departments have led to a successful outcome and encourages us in our daily struggle in this often demanding surgery.


Assuntos
Aorta , Aneurisma Aórtico/cirurgia , Dissecção Aórtica/cirurgia , Complicações Cardiovasculares na Gravidez/cirurgia , Adulto , Dissecção Aórtica/diagnóstico por imagem , Aorta Torácica , Aneurisma Aórtico/diagnóstico por imagem , Prótese Vascular , Implante de Prótese Vascular , Cesárea , Angiografia por Tomografia Computadorizada , Procedimentos Endovasculares , Feminino , Idade Gestacional , Humanos , Histerectomia , Comunicação Interdisciplinar , Equipe de Assistência ao Paciente , Gravidez , Complicações Cardiovasculares na Gravidez/diagnóstico por imagem , Segundo Trimestre da Gravidez , Stents , Resultado do Tratamento
19.
Cardiovasc Intervent Radiol ; 41(7): 1074-1080, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-29687260

RESUMO

PURPOSE: The annual permissible radiation ocular lens dose has been reduced to 20 millisieverts (mSv) in the current European directive 2013/59/Euratom. The aim of this study was to evaluate the personal radiation dose for vascular interventions with special focus on ocular lens dose. MATERIALS AND METHODS: From May 2016 to October 2016, the personal radiation doses of two interventionists and four technicians were prospectively recorded during 206 vascular interventions. The position of personnel, intervention type and fluoroscopy time were recorded. Parameters evaluated were total body dose measured by film dosimeter, hand dose measured by ring thermoluminescent dosimeter (TLD) and ocular lens dose measured by TLD placed in front of the safety glasses. Linear regression analysis was used to estimate the dose at 2 and 5 years. RESULTS: The ocular lens dose, hand and total body dose of the two interventionists were 11/5, 56/47 and 0.6 mSv each, respectively. The estimated 5-year ocular dose was 113.08 mSv (95% CI 38.2-187.97)/40.95 (95% CI 16.9-64.7). Similarly, hand dose was 608.4 mSv (95% CI 442.78-774.38)/514.47 (95% CI 329.83-699.10) and body dose 6.07 mSv (95% CI 4.70-8.22)/5.12 (95% CI 3.65-6.59), respectively. Amongst four technicians, only the first assistant showed recordings of 0.3 mSv body dose, 2 mSv ocular lens dose and 5 mSv hand dose. CONCLUSION: The yearly ocular lens dose, particularly for interventionists dealing with complex interventions, could cross the permitted yearly limit set by the new Euratom directive. Therefore, X-ray safety glasses would become mandatory for complex radiological vascular interventions. LEVEL OF EVIDENCE: Level III, non-randomized controlled cohort/follow-up study.


Assuntos
Dispositivos de Proteção dos Olhos , Exposição Ocupacional/prevenção & controle , Lesões por Radiação/prevenção & controle , Proteção Radiológica/instrumentação , Radiografia Intervencionista/efeitos adversos , Doenças Vasculares/terapia , Europa (Continente) , Feminino , Fluoroscopia/efeitos adversos , Seguimentos , Guias como Assunto , Humanos , Estudos Prospectivos , Dosímetros de Radiação , Fatores de Tempo
20.
J Thorac Dis ; 10(11): 6192-6200, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30622791

RESUMO

BACKGROUND: Surgical management of aortic arch diseases is one of the most challenging issues in cardiovascular surgery. The aim of this study was to compare the outcome after frozen elephant trunk (FET) with conventional elephant trunk (ET) technique. METHODS: Out of a total of 551 patients after thoracic aortic surgery, we analyzed 70 consecutive patients, who underwent aortic arch replacement with ET or FET technique between 2001 and 2017 in our institution. The patients were case-control matched in regard to such variables as age, sex, presence of an acute aortic dissection and necessity for concomitant procedures. The analysis included 25 patient pairs. RESULTS: Among the 25 FET patients, eleven patients were female, the median age was 69, 15 (60%) patients had an aortic dissection and thirteen needed various concomitant procedures. In the second group, treated with conventional ET technique, 10 (40%) patients were female, the median age was 66 years, thirteen presented with an aortic dissection and 16 (64%) underwent concomitant procedures. These and other characteristics did not differ significantly between the groups. In-hospital mortality was statistically similar: 5 (20%) in the FET group vs. 8 (32%) for ET group (P=0.52). The incidence of stroke, acute renal failure and postoperative bleeding was comparable. The length of stay in the intensive care unit did not differ between the cohorts (P=0.258). Predictors of in-hospital mortality were length of the operation, bleeding postoperatively, and acute renal failure. The one-year survival rates were higher in the FET cohort compared to the conventional approach (60% vs. 38%), however without statistical significance. CONCLUSION: In regard to the short- and mid-term outcome, there were only slight differences between both techniques. In patients with extensive aneurysmal aortic disease, conventional ET and FET procedures seem to be associated with acceptable satisfactory mid-term outcome.

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