Examining thermally induced movement of the fatal fire victim.

Investigating a fatal fire scene comprises analysis not only of the fire's development to identify the point of fire origin and ignition source, but analysis of a victim's position and their relationship within the scene. This work presents both qualitative and quantitative results from experimentation investigating the effect of a real fire environment on the human body, and how the position of a victim at the post burn investigation stage may be significantly different to the position at fire ignition. Qualitative observations were undertaken on the burning of 39 compartment and vehicle scenes from ignition through to suppression, each containing a human cadaver. The results of analysis question the validity of previous work based on cremation observations. Quantitative results were produced by recording 13 points on the body on the X, Y and Z axis, both pre and post burn on a smaller dataset of ten compartment burns. Results have enabled a more robust assessment of thermally induced movement of the body within the scene along each axis, evidencing that pugilism is not the universal reaction of the fatal victim to thermal exposure, with extension of the upper limbs far more common than has been previously reported.

Examination of misconceptions surrounding fatal fire victims.

Fire deaths are not unusual in forensic investigative practice but due to the destructive nature of fire they are often very difficult to investigate. With the need to identify the deceased and the events surrounding the manner and cause of death, knowledge of thermally induced alteration to the human body is important. Within the fire investigation community, a number of misconceptions have been present for years regarding the protrusion of the tongue as an indicator of life during the fire, and fractured skulls as the result of brains boiling and skulls exploding. This work presents qualitative analysis on the experimental burning of 42 unembalmed human donated cadavers by the San Luis Obispo Strike Team (SLOFIST) on their annual Forensic Fire Death Investigation Course (FFDIC) between 2017 and 2019. Prior to burning, the position of the tongue within the dental arch was confirmed and sharp, blunt, surgical and gunshot trauma to the cranium documented. Temperature was recorded from ignition through to suppression with thermocouples present both within the scene and the body. Post burn analysis on the position of the tongue, observation of cranial fractures and presence of brain tissue were recorded and analysed in conjunction with thermocouple data, fire scene dynamics and body demographics. The results provide a more comprehensive understanding of the thermal environmental factors involved in producing the phenomena that facilitate these misconceptions, identifying that a more thorough understanding of individual fire scenes and their development is essential when interpreting alteration and injury to the body of the fatal fire victim.

Quantification of the variability and penetration of per- and poly-fluoroalkyl substances through a concrete pad.

Historical use of aqueous film forming foams (AFFF) containing per- and poly-fluoroalkyl substances (PFAS) for fire-fighting activities has contributed to widespread contamination of infrastructure which can represent an ongoing source of PFAS to the surrounding environment. A concrete fire training pad with historical use of Ansulite and Lightwater AFFF formulations had PFAS concentrations measured to quantify spatial variability of PFAS within the pad. Surface chips and whole cores of concrete through to the underlying aggregate base were collected over the 24 × 9 m concrete pad and depth profiles of PFAS concentrations in nine cores were analysed. PFOS and PFHxS dominated the PFAS for surface samples, along the depth profile of cores and in the underlying plastic and aggregate material, with substantial variability in the concentrations of PFAS in the samples. Although there was variability of individual PFAS along the depth profile, higher surface concentrations of PFAS generally followed the designed movement of water across the pad. Total oxidisable precursor (TOP) assessments of one core indicated additional PFAS were present along the entire length of the core. This study highlights concentrations of PFAS (up to low µg/kg) from historical use of AFFF can occur throughout concrete, with the variable concentrations throughout the profile.

Correction: Method for extraction and analysis of per- and poly-fluoroalkyl substances in contaminated asphalt.

Correction for 'Method for extraction and analysis of per- and poly-fluoroalkyl substances in contaminated asphalt' by Prashant Srivastava et al., Anal. Methods, 2022, 14, 1678-1689, https://doi.org/10.1039/D2AY00221C.

Sorption, degradation and microbial toxicity of chemicals associated with hydraulic fracturing fluid and produced water in soils.

Spills of hydraulic fracturing (HF) fluids and of produced water during unconventional gas extraction operations may cause soil contamination. We studied the degradation and microbial toxicity of selected HF chemical components including two biocides (methylisothiozolinone- MIT, chloromethylisothiozolinone- CMIT), a gel-breaker aid (triethanolamine -TEA), and three geogenic chemicals (phenol, m-cresol and p-cresol) in ultrapure water, HF fluid and produced water in five different soil types (surface and subsurface soils). The degradation of the two biocides (in soils treated with HF fluid or ultrapure water) and of the three geogenic chemicals (in soils treated with produced water) was rapid (in all cases DT50 values < 2 days in surface soils). In contrast, the loss of TEA was much slower in soils, especially in those treated with HF fluid (DT50 > 30 days). Sorption coefficients (Koc in L/Kg) in these soils ranged from 71 to 733 for TEA, 64-408 for MIT and 11-72 for CMIT. In terms of soil microbial toxicity, exposure to HF fluid and produced water reduced microbial respiration, albeit temporarily. The overall microbial activities in surface soils contaminated with produced water had fully recovered in most soils. In contrast, the HF fluid addition to soils completely inhibited the nitrification in all soils, with little recovery over the 60 day experimental period. In the case of produced water exposure, three out of five surface soils showed complete recovery in nitrification during the study period. The functional genes for nitrogen fixation (nifH) and carbon cycling (GA1) and microbial community composition (16 S rRNA) were significantly affected by HF fluid in some soils. Overall, the study shows that the HF fluid can have significant detrimental impact on soil microbial functions, especially on nitrogen cycling. More work is needed to identify the exact cause of microbial toxicity in soils contaminated with HF fluid.

Method for extraction and analysis of per- and poly-fluoroalkyl substances in contaminated asphalt.

The legacy use of aqueous film-forming foam (AFFF) has led to the generation of large volumes of per- and poly-fluoroalkyl substances (PFAS)-contaminated asphalt materials, especially at airports and fire training areas. The management of such PFAS-contaminated asphalt materials requires an understanding of PFAS concentrations in these materials. This study, therefore, aimed to develop a suitable extraction methodology for the analysis of 22 target PFAS (i.e., carboxylic acids, sulfonic acids and fluorotelomers) in asphalt materials. A series of experiments was conducted to optimise extraction solvent composition, as well as to assess the performance of the chosen method under various conditions (i.e., sonication temperature, PFAS contamination level, asphalt core composition and timing of stable isotope addition used as internal standard). The methanol-based extractants performed best due to their accuracy and precision, which were within the acceptable range (extraction efficiency between 70 and 130% and RSD < 20%). The method which involved three successive extractions with methanol/1% NH3 by ultrasonication at 25 °C was selected due to its performance and ease of operation. The mean recovery of a vast majority of PFAS was found to be in the acceptable range. Tests on the timing of addition of stable isotope (SI)-labelled PFAS internal standards indicate that the recoveries obtained, regardless of when the stable isotopes were added, were within the acceptable range for PFAS. The accuracy and precision of PFAS recoveries were not affected by PFAS spike level (2 µg kg-1 and 200 µg kg-1), as well as sample composition (based on the location of asphalt material in the field). Low RSDs were achieved for asphalt cores collected from a contaminated site covering a wide range of concentrations (from LOQ to 2135 mg kg-1), demonstrating the suitability of the sample preparation method for real-world samples. The results from the interlaboratory testing were also in good agreement and validated the proposed PFAS extraction and analytical approach.

Osteochondral Lesions of the Ankle Treated with Bone Marrow Concentrate with Hyaluronan and Fibrin: A Single-Centre Study.

Osteochondral defects of the ankle (OCD) are being increasingly identified as a clinically significant consequence of injury to the ankle, with the potential to lead to osteoarthritis if left untreated. The aim of this retrospective cohort study was to evaluate a single-stage treatment of OCD, based on bone marrow aspirate (BMA) centrifuged to produce bone marrow concentrate (BMC). In a dual syringe, the concentrate was mixed with thrombin in one syringe, whereas hyaluronan and fibrinogen were mixed in a second syringe. The two mixtures were then injected and combined into the prepared defect. Clinical outcome and quality of life scores (MOXFQ and EQ-5D) were collected at baseline and yearly thereafter. Multilevel models were used to analyse the pattern of scores over time. Ninety-four patients were treated between 2015 and 2020. The means of each of the three components of the MOXFQ significantly improved between baseline and 1 year (p < 0.001 for each component), with no further change from year 1 to year 3. The EQ-5D index also improved significantly from baseline to 1 year, with no evidence for further change. Our results strongly indicate that this BMC treatment is safe for, and well tolerated by, patients with OCD of the ankle as both primary treatment and those who have failed primary treatment. This technique provides a safe, efficacious alternative to currently employed cartilage repair techniques, with favourable outcomes and a low complication rate at 36 months.

Application of native plants in constructed floating wetlands as a passive remediation approach for PFAS-impacted surface water.

Strategies for remediation of per- and polyfluoroalkyl substances (PFAS) generally prioritise highly contaminated source areas. However, the mobility of PFAS in the environment often results in extensive low-level contamination of surface waters across broad areas. Constructed Floating Wetlands (CFWs) promote the growth of plants in buoyant structures where pollutants are assimilated into plant biomass. This study examined the hydroponic growth of Juncus krausii, Baumea articulata and Phragmites australis over a 28-day period for remediation of perfluorooctanoic acid (PFOA) and perfluorooctane sulfonic acid (PFOS) contaminated (0.2 µg/L to 30 µg/L) urban stormwater. With increasing PFOA and PFOS concentrations, accumulation in plant species increased although root and shoot distribution varied depending on PFAS functional group. Less PFOA than PFOS accumulated in plant roots (0.006-0.16 versus 0.008-0.68 µg/g), while more PFOA accumulated in the plant shoots (0.02-0.55 versus 0.01-0.16 µg/g) indicating translocation to upper plant portions. Phragmites australis accumulated the highest overall plant tissue concentrations of PFOA and PFOS. The NanoSIMS data demonstrated that PFAS associated with roots and shoots was absorbed and not just surface bound. These results illustrate that CFWs have the potential to be used to reduce PFAS contaminants in surface waters.

Modelling the attenuation of flowback chemicals for a soil-groundwater pathway from a hypothetical spill accident.

Flowback water from shale gas operations contains formation-derived compounds, including trace metals, radionuclides, and organics. While accidental releases from storage tanks with flowback water are low-probability events if multiple containment barriers are put in place, they cannot be entirely excluded. Here the natural attenuation potential of deep unsaturated zones and groundwater was explored using predictive modelling involving a hypothetical leak from a storage tank. Actual chemical concentrations from flowback water at two shale gas wells with contrasting salinity (12,300 and 105,000 ppm TDS) in the Beetaloo Sub-basin (Northern Territory, Australia) served as input to the one-dimensional HYDRUS model for simulating chemical transport through the unsaturated zone, with groundwater at 50 and 100 m depth, respectively. Subsequent chemical transport in groundwater involved the use of a three-dimensional analytical transport model. For a total of 63 chemicals the long-term attenuation from dilution and dispersion in unsaturated sediments and groundwater was calculated. Predicted environmental concentrations for aquatic receptors were compared with no-effect levels of individual chemicals to derive risk quotients (RQ) and identify chemicals of no concern to ecosystem health (i.e. RQ <1). Except for salinity and radium-228 in one of the two wells, RQ < 1 for all other chemicals. The initial approach considered testing of toxicity to individual chemicals only. When direct toxicity assessments (DTAs) were used to account for effects of chemical mixtures, the required DTA-derived safe dilution factor for 95% species protection was 1.8 to 2.5 times higher than the dilution factor accounting for dispersion and dilution only. Accounting for biodegradation, sorption and radioactive decay decreased chemical concentrations in unsaturated sediments to safe levels using the DTA for all chemicals. The study highlighted the importance of incorporating DTA in chemical risk assessments involving complex chemical mixtures. Improved understanding of fate and transport of flowback chemicals will help effectively manage water-quality risks associated with shale gas extraction.

Sensory organization for postural control in children with strabismus-A systematic review and meta-analysis.

BACKGROUND: Postural control requires the interaction of somatosensory, vestibular and visual systems to prevent disequilibrium. Children with strabismus have an impaired visual input which can lead to postural control deficits. RESEARCH QUESTION: Does strabismus affect sensory organization for postural control in children? METHODS: A systematic comprehensive search of multiple electronic databases for relevant articles was performed using a predetermined search strategy. Peer-reviewed journal publications that assessed sensory organization and postural performance in children with strabismus were included in this review. Methodological quality of the articles was evaluated using the modified Downs and Black quality assessment tool. A total of 10 articles were included in this review. RESULTS: Eight of the 10 articles reviewed were included in the meta-analysis. Meta-analysis for Centre of Pressure CoP velocity (n = 3) (eyes open, stable support), was statistically significant (P = 0.01) in favor of controls over children with strabismus (MD, 3.08; 95 % CI -0.66, 5.51). Meta-analysis for CoP surface area (n = 5) (eyes open, stable support) was also statistically significant (P < 0.001) in favor of the control group (MD, 130.14; 95 % CI 70.01, 190.260). Meta-analysis with standardized mean difference (n = 6) for eyes open vs closed, stable support was statistically significant (P = 0.02) in favor of eyes open (MD, -0.94; 95 % CI, -1.74, -0.15). Overall, there was a high level of heterogeneity throughout the studies. SIGNIFICANCE: This is the first systematic review of its kind to collate and synthesize evidence on the effect of strabismus on postural control in children. This review suggests that postural control performance in children with strabismus is inferior to that of age-matched control children. Further investigation is needed to understand the influence of the three sensory systems.

Effect of dietary coarse corn inclusion on broiler live performance, litter characteristics, and ammonia emission.

Ammonia (NH3) emission from nondigested nutrients in poultry creates additional adverse environmental impacts on soil, water, air, and health. Mitigating NH3 emission has become vital for the poultry industry to remain sustainable. As the presence of large particles in the feed stimulates the broiler gizzard to retain ingesta in the gastrointestinal tract longer and improve digestive efficiency, the inclusion of large particles in feed may lead to less nitrogen (N) and moisture content (MC) in feces such that lower NH3 production would be expected. This chamber study investigated the effects of dietary coarse corn (CC) inclusion on broiler live performance, litter characteristics, and NH3 emission. One hundred eighty female broilers (Ross 344 × 708 strains) at day 21 were randomly placed in 6 chambers with 2 dietary treatments (0% CC and 50% CC), with 3 chambers per treatment and 30 birds per chamber for 3 wks. The results showed that the 50% CC inclusion (1) decreased broiler feed intake and BW without affecting mortality-adjusted feed conversion ratio from day 21 to 42; (2) increased gizzard weight and decreased proventriculus weight; (3) decreased N content and MC in litter; and (4) decreased NH3 concentrations in the chambers, as well as NH3 emission from the chambers. Dietary CC inclusion could be an effective way to mitigate broiler litter N content and MC as well as NH3 emission.

Sources, presence and potential effects of contaminants of emerging concern in the marine environments of the Great Barrier Reef and Torres Strait, Australia.

Current policy and management for marine water quality in the Great Barrier Reef (GBR) in north-eastern Australia primarily focusses on sediment, nutrients and pesticides derived from diffuse source pollution related to agricultural land uses. In addition, contaminants of emerging concern (CECs) are known to be present in the marine environments of the GBR and the adjacent Torres Strait (TS). Current and projected agricultural, urban and industrial developments are likely to increase the sources and diversity of CECs being released into these marine ecosystems. In this review, we evaluate the sources, presence and potential effects of six different categories of CECs known to be present, or likely to be present, in the GBR and TS marine ecosystems. Specifically, we summarize available monitoring, source and effect information for antifouling paints; coal dust and particles; heavy/trace metals and metalloids; marine debris and microplastics; pharmaceuticals and personal care products (PPCPs); and petroleum hydrocarbons. Our study highlights the lack of (available) monitoring data for most of these CECs, and recommends: (i) the inclusion of all relevant environmental data into integrated databases for building marine baselines for the GBR and TS regions, and (ii) the implementation of local, targeted monitoring programs informed by predictive methods for risk prioritization. Further, our spatial representation of the known and likely sources of these CECs will contribute to future ecological risk assessments of CECs to the GBR and TS marine environments, including risks relative to those identified for sediment, nutrients and pesticides.

Translocation of pharmaceuticals from wastewater into beehives.

There has been a substantial research focus on the presence of pesticides in flowers and the subsequent exposure to honeybees. Here we demonstrate for the first time that honeybees can also be exposed to pharmaceuticals, commonly present in wastewater. Residues of carbamazepine (an anti-epileptic drug) up to 371 ng/mL and 30 µg/g were detected in nectar and pollen sampled from zucchini flowers (Cucurbita pepo) grown in carbamazepine spiked soil (0.5-20 µg/g). Under realistic exposure conditions from the use of recycled wastewater, carbamazepine concentrations were estimated to be 0.37 ng/L and 30 ng/kg in nectar and pollen, respectively. Incorporation of environmentally relevant carbamazepine residues in nectar and pollen into a modelling framework able to simulate beehive dynamics including the honeybee foraging activity at the landscape scale (BEEHAVE and BEESCOUT) enabled the simulation of carbamazepine translocation from zucchini fields into honeybee hives. Carbamazepine accumulation was modelled in 11 beehives across a 25 km2 landscape over three years chosen to represent distinct climatic conditions. During a single flowering period, carbamazepine concentrations were simulated to range between 0 and 2478 ng per beehive. The amount of carbamazepine gathered not only varied across the simulated years but there were also differences in accumulation of carbamazepine between beehives within the same year. This work illustrates a fundamental first step in assessing the risk of pharmaceuticals to bees through realistic scenarios by demonstrating a method to quantify potential exposure of honeybees at the landscape scale. Pharmaceuticals are being inadvertently but increasingly applied to agricultural lands globally via the use of wastewater for agricultural irrigation in response to water scarcity problems. We have demonstrated a route of pharmaceutical exposure to honeybees via contaminated nectar and pollen. Given the biological potency of pharmaceuticals, accumulation of these chemicals in nectar and pollen suggest potential implications for honeybee health, with unknown ecosystem consequences.

Photoproduction of Axionlike Particles.

We explore the sensitivity of photon-beam experiments to axionlike particles (ALPs) with QCD-scale masses whose dominant coupling to the standard model is either to photons or gluons. We introduce a novel data-driven method that eliminates the need for knowledge of nuclear form factors or the photon-beam flux when considering coherent Primakoff production off a nuclear target, and show that data collected by the PrimEx experiment in 2004 could improve the sensitivity to ALPs with 0.03≲m_{a}≲0.3 GeV by an order of magnitude. Furthermore, we explore the potential sensitivity of running the GlueX experiment with a nuclear target and its planned PrimEx -like calorimeter. For the case where the dominant coupling is to gluons, we study photoproduction for the first time, and predict the future sensitivity of the GlueX experiment using its nominal proton target. Finally, we set world-leading limits for both the ALP-gluon coupling and the ALP-photon coupling based on public mass plots.

Coupling QCD-Scale Axionlike Particles to Gluons.

We present a novel data-driven method for determining the hadronic interaction strengths of axionlike particles (ALPs) with QCD-scale masses. Using our method, it is possible to calculate the hadronic production and decay rates of ALPs, along with many of the largest ALP decay rates to exclusive final states. To illustrate the impact on QCD-scale ALP phenomenology, we consider the scenario where the ALP-gluon coupling is dominant over the ALP coupling to photons, electroweak bosons, and all fermions for m_{π}≲m_{a}≲3 GeV. We emphasize, however, that our method can easily be generalized to any set of ALP couplings to standard model particles. Finally, using the approach developed here, we provide calculations for the branching fractions of η_{c}→VV decays; i.e., η_{c} decays into two vector mesons, which are consistent with the known experimental values.

Microplastics in municipal mixed-waste organic outputs induce minimal short to long-term toxicity in key terrestrial biota.

Sustainable alternatives to landfill disposal for municipal mixed wastes represents a major challenge to governments and waste management industries. In the state of New South Wales (NSW) Australia, mechanical biological treatment (MBT) is being used to reduce the volume and pathogen content of organic matter isolated from municipal waste. The product of this treatment, a compost-like output (CLO) referred to as mixed waste organic output (MWOO), is being recycled and applied as a soil amendment. However, the presence of contaminants in MWOO including trace organics, trace metals and physical contaminants such as microplastic fragments has raised concerns about potential negative effects on soil health and agriculture following land application. Here, we used multiple lines of evidence to examine the effects of land application of MWOO containing microplastics in three soils to a variety of terrestrial biota. Treatments included unamended soil, MWOO-amended soil and MWOO-amended soil into which additional high-density polyethylene (HDPE), polyethylene terephthalate (PET), or polyvinyl chloride (PVC) microplastics were added. Tests were conducted in soil media that had been incubated for 0, 3 or 9 months. Addition of microplastics had no significant negative effect on wheat seedling emergence, wheat biomass production, earthworm growth, mortality or avoidance behaviour and nematode mortality or reproduction compared to controls. There was also little evidence the microplastics affected microbial community diversity, although measurements of microbial community structure were highly variable with no clear trends.

Emerging contaminants in a river receiving untreated wastewater from an Indian urban centre.

Research over the last decade on emerging trace organic contaminants in aquatic systems has largely focused on sources such as treated wastewaters in high income countries, with relatively few studies relating to wastewater sources of these contaminants in low and middle income countries. We undertook a longitudinal survey of the Ahar River for a number of emerging organic contaminants (including pharmaceuticals, hormones, personal care products and industrial chemicals) which flows through the city of Udaipur, India. Udaipur is a city of approximately 450,000 people with no wastewater treatment occurring at the time of this survey. We found the concentrations of many of the contaminants within the river water were similar to those commonly reported in untreated wastewater in high income countries. For example, concentrations of pharmaceuticals, such as carbamazepine, antibiotics and non-steroidal anti-inflammatory drugs, ranged up to 1900 ng/L. Other organic contaminants, such as steroid estrogens (up to 124 ng/L), steroid androgens (up to 1560 ng/L), benzotriazoles (up to 11 µg/L), DEET (up to 390 ng/L), BPA (up to 300 ng/L) and caffeine (up to 37.5 µg/L), were all similar to previously reported concentrations in wastewaters in high income countries. An assessment of the population densities in the watersheds feeding into the river showed increasing population density of a watershed led to a corresponding downstream increase in the concentrations of the organic contaminants, with quantifiable concentrations still present up to 10 km downstream of the areas directly adjacent to the highest population densities. Overall, this study highlights how a relatively clean river can be contaminated by untreated wastewater released from an urban centre.