Determinants and constraints of carrot (Daucus carota L.) production and marketing in Cameroon.

The market gardening sector in Cameroonian agriculture is facing a challenge in producing vegetables to meet consumer needs. Among these vegetables, carrot is known to play an important role in the livelihood of millions of people around the world. It is therefore important to understand its technical itinerary in the different agricultural basins in order to provide further information on carrot production. This study aimed to identify the different production constraints faced by carrot growers by providing information on applied growing systems. Two surveys were conducted using a questionnaire simultaneously with carrot traders and producers. A total of 218 carrot traders and 62 producers were interviewed. Nine basins were identified as main carrot production zones in Cameroon. In the production basins, five main varieties namely New Kuroda, Pamela+, Madona, Amazonia and Vanessa F1 were identified. The agricultural yield of carrots is related to the growing area, fertilization method, size of the field and ploughing. Farmers use an integrated fertilization approach based on chemical fertilizer (N-P-K: 20-10-10) and chicken manure at various doses, from 250 to 500 Kg.ha-1 and 2.5 to 9 t.ha-1 respectively. Factors such as farmland area, number of cultivated plots, experience in cultivation, family size and amounts of organic and chemical fertilizers used have been found to have significant impact on carrot production. However, among the many technical problems faced by producers and which result in low carrots yields, is the lack of knowledge of soil properties. This in turn contributes to inappropriate fertilization and poor choice of the appropriate variety to be cultivated. Low germination, the lack of efficient irrigation systems and the high costs of agricultural inputs are the main constraints that affect carrot production. Although valued by market gardeners, the benefit in a production season is not always enough to encourage more farmers to grow carrots. Thus, there is a need to develop a follow-up policy for the quality and high yield production of the carrot sector.

Contribution of community-based organizations in the improvement of Joint United Nations Program on HIV and AIDS 90-90-90: case of the Yaoundé Central Hospital.

Community-based organizations (CBOs) are one of the initiatives implemented in Cameroon to improve access to antiretroviral treatment and influence retention in treatment centers. Despite its importance in the decongestion of patients in health facilities, we do not have data to evaluate the overall impact of these organizations. We conducted a two-part observational study. The first part was a descriptive cross-sectional study, where we included patients screened and initiated on anti-retroviral treatment (ART) either by the approved Treatment center (ATC) of Yaoundé Central Hospital (YCH) or by any of our CBOs in 2020. Then, the second part was a retrospective cohort-type study including patients from the 2015 cohort followed up from 2018 to 2020 in order to assess viral load suppression. As regards the first "90", 7,234 screening tests were performed by CBOs in 2020 out of the 28,302 screening tests registered at the YCH, giving a contribution of 25.6%. From the 7,234 screening tests performed by CBOs, 314 people had an HIV-positive result and 230 (73.34%) were linked to ART through CBOs. From the 28,302 screening tests performed at YCH, 1,089 people had an HIV-positive test, and only 354 (32.50%) were linked to ART, giving a significant difference in the link to ART (P-value < 0.00). Concerning the 3rd ''90'', the viral load suppression rates were respectively in CBOs and at YCH of (95.12% vs 90.54%, RR= 0.51; P-value= 0.27 at 12 months); (95.96% vs 95.34%, relative risk (RR)= 0.85; P-value= 0.81 at 24 months); and (96.91% vs 94.15%, RR= 0.52; P-value = 0.24 at 36 months). In conclusion, we say that the follow-up of patients living with HIV in the community does not negatively affect the evolution of the disease as one might think.

Optimization of the Processing Conditions for the Production of a Gluten-Free Bread from Sour Cassava Starch (Manihot esculenta) and Some Legumes (Arachis hypogaea, Vigna unguiculata, and Glycine max).

BACKGROUND: Sour cassava starch is used as an alternative to wheat flour in breadmaking. However, its nutritional and technological properties are limited. To remedy this, the use of legumes has proved to be very successful. Thus, the present study aimed to determine the optimal condition for the production of bread made from sour cassava starch, peanut, cowpea and soybean flour. METHODS: The I-optimal design was employed to obtain an optimal proportion of the mixture with the variables sour cassava starch, cowpea, soy and peanut flour. The responses evaluated were overall acceptability, specific volume and protein content. RESULTS: It resulted that the incorporation of sour cassava starch positively influenced the volume but negatively influenced the protein content and overall acceptability. While the addition of legumes increased protein content and overall consumer acceptability, the specific volume was reduced. The optimal proportions of sour cassava starch, cowpea, soybean and peanut flour were 64.11%, 18.92%, 0% and 16.96%, respectively. Under this condition, it led to a desirability of 1, specific volume of 1.35, overall acceptability of 6.13, protein content of 9.72%, carbohydrate content of 67.89%, fat content of 9.39%, fiber content of 2.10% and ash content of 1.04%. CONCLUSIONS: The findings suggest that cowpea and peanut can be used for the improvement of the technological, nutritional and sensory properties of sour cassava starch bread and thus increase its consumption and application in the food processing industry.

Physicochemical properties, nutritional value, and antioxidant potential of jackfruit (Artocarpus heterophyllus) pulp and seeds from Cameroon eastern forests.

This work aimed to study the physicochemical, nutritional, and antioxidant characteristics of the pulp and seeds of the jackfruit collected in the East Cameroon region to reduce the malnutrition problems encountered in the said region. To achieve this work, we first administered a survey. We observed that 50 people from Bertoua knew the fruit among the 200 people surveyed. After that, the physicochemical characteristics and nutritional value of Artocarpus heterophyllus pulp and seeds from Bertoua were evaluated. Finally, the antioxidant compounds (total phenolic and flavonoid contents) and the antioxidant activities were evaluated through the DPPH free radical scavenging capacity, the ability to reduce ferric ions, and the hydroxyl radical scavenging ability using the aqueous, ethanolic, and hydro-ethanolic extracts of these samples (pulp and seeds). Results (means ± standard deviation) were obtained in triplicate and analyzed statistically by the analysis of variance (ANOVA) at the 0.05 probability level. The results revealed that the jackfruit pulp has a water content (89.85% ± 0.49) significantly higher than that of seeds (60.075% ± 0.12). The pH of jackfruit pulp (5 ± 0) is significantly lower than that of seeds (6 ± 0). Jackfruit pulp had a carbohydrate content (54.39% ± 0.47) significantly higher than that of seeds (49.01% ± 0.43). The protein content of jackfruit pulp (18.35% ± 0.04) is lower than that of the seeds (21.66% ± 0.31). For mineral content, the maximum contents of K (848 mg/100 g ± 10.34) and Na (69.53 mg/100 g ± 0) were identified in the jackfruit pulp while those of Ca (132 mg/100 g ± 9.42), Mg (43.73 mg/100 g ± 9.12), and P (101.51 mg/100 g ± 4.02) were found in the seeds. However, all extracts possessed both antioxidant compounds (phenols and flavonoids) and antioxidant activities. In conclusion, the jackfruit appears to be a fruit that can improve the nutritional status of the populations of eastern Cameroon.

In Vitro Evaluation of the Probiotic and Safety Properties of Bacteriocinogenic and Non-Bacteriocinogenic Lactic Acid Bacteria from the Intestines of Nile Tilapia and Common Carp for Their Use as Probiotics in Aquaculture.

In this study, seven bacteriocinogenic and non-bacteriocinogenic LAB strains previously isolated from the intestines of Nile tilapia and common carp and that showed potent antibacterial activity against host-derived and non-host-derived fish pathogens were assayed for their probiotic and safety properties so as to select promising candidates for in vivo application as probiotic in aquaculture. All the strains were investigated for acid and bile tolerances, transit tolerance in simulated gastrointestinal conditions, for cell surface characteristics including hydrophobicity, co-aggregation and auto-aggregation, and for bile salt hydrolase activity. Moreover, haemolytic, gelatinase and biogenic amine-producing abilities were investigated for safety assessment. The strains were found to be tolerant at low pH (two strains at pH 2.0 and all the strains at pH 3.0). All of them could also survive in the presence of bile salts (0.3% oxgall) and in simulated gastric and intestinal juices conditions. Besides, three of them were found to harbour the gtf gene involved in pH and bile salt survival. The strains also showed remarkable cell surface characteristics, and 57.14% exhibited the ability to deconjugate bile salts. When assayed for their safety properties, the strains prove to be free from haemolytic activity, gelatinase activity and they could neither produce biogenic amines nor harbour the hdc gene. They did not also show antibiotic resistance, thus confirming to be safe for application as probiotics. Among them, Lactobacillus brevis 1BT and Lactobacillus plantarum 1KMT exhibited the best probiotic potentials, making them the most promising candidates.

Quantitative analyses of the bacterial microbiota of rearing environment, tilapia and common carp cultured in earthen ponds and inhibitory activity of its lactic acid bacteria on fish spoilage and pathogenic bacteria.

The present study aimed to evaluate the bacterial load of water, Nile Tilapia and common Carp intestines from earthen ponds, isolate lactic acid bacteria (LAB) and assess their antimicrobial activity against fish spoilage and pathogenic bacteria. Following enumeration and isolation of microorganisms the antimicrobial activity of the LAB isolates was evaluated. Taxonomic identification of selected antagonistic LAB strains was assessed, followed by partial characterisation of their antimicrobial metabolites. Results showed that high counts (>4 log c.f.u ml-1 or 8 log c.f.u g-1) of total aerobic bacteria were recorded in pond waters and fish intestines. The microbiota were also found to be dominated by Salmonella spp., Vibrio spp., Staphylococcus spp. and Escherichia coli. LAB isolates (5.60%) exhibited potent direct and extracellular antimicrobial activity against the host-derived and non host-derived spoilage and pathogenic bacteria. These antagonistic isolates were identified and Lactococcus lactis subsp. lactis was found as the predominant (42.85%) specie. The strains displayed the ability to produce lactic, acetic, butyric, propionic and valeric acids. Bacteriocin-like inhibitory substances with activity against Gram-positive and Gram-negative (Vibrio spp. and Pseudomonas aeruginosa) bacteria were produced by three L. lactis subsp. lactis strains. In this study, the LAB from the microbiota of fish and pond water showed potent antimicrobial activity against fish spoilage or pathogenic bacteria from the same host or ecological niche. The studied Cameroonian aquatic niche is an ideal source of antagonistic LAB that could be appropriate as new fish biopreservatives or disease control agents in aquaculture under tropical conditions in particular or worldwide in general.

Evaluation of the nutritional status of infants from mothers tested positive to HIV/AIDS in the health district of Dschang, Cameroon.

INTRODUCTION: Poor infant feeding practices are common in Africa, resulting in physical and intellectual developmental impairments. Good feeding practices are crucial, especially in the first year of growth. HIV/AIDS has worsened the clinical and nutritional status of both mothers and their children, exacerbating high rates of malnutrition. The aim of this study was to assess by participative approach, the nutritional status of infants from mothers tested positive to HIV in the health district of Dschang. METHODS: This is a cross sectional study with a period of recruitment of 2 years (2010-2012). Data Collection was done by the aim of a personal slip followed by training to strengthen the nutritional and hygienic capacity of targeted parents. Height and weight of infants were measured and body mass index (BMI) calculated. RESULTS: Significant difference (p ≤ 0.05) was noticed in height-for-age z-score (HAZ) of girls aged between 1 to 2 years compared to 1-year old girls as well as to boys of all ages, defining them as stunted. Furthermore, the weight-for-age z-score (WAZ) results indicate that both girls and boys of all age are in moderate state of malnutrition. The results of BMI thinness classified according to gender and age groups, indicates that most infants (68/130, 52.3%) showed grade 2 thinness predominantly in 2-years old both boys and girls. However, no participants fall within the normal category for age and sex, as well as overweight and obesity categories. CONCLUSION: Undernutrition exists among infants from mothers tested positive to HIV residing in Dschang, as most of the infants are underweight, and malnourished.